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노랑초파리 ecdysone 결핍 돌연변이(ecd1ts)에서 기능적 ecdysone수용체 구성분의 발현
김세제,박지권,윤원식,이영기,고영상 제주대학교 생명과학연구소 1998 제주생명과학연구 Vol.1 No.-
In Drosophila, the steroid hormone ecdysone triggers the key regulatory cascades controlling the coordinated changes in the developmental pathway of molting and metamorphosis. Ecdysone action is mediated by heterodimer consisting of the ecdysone receptor(EcR) and ultraspiracle proteins(Usp). Heterodimers of these proteins bind to ecdysone response element and ecdsone to modulate gene transcription. In this study, the transcripts of functional ecdsone receptors and ecdysone response genes(Fbp1 and Uro) in ecdysoneless(ecd-1ts) mutation were analyzed by using a RT-PCR and competitive RT-PCR method. The expression of functional ecdysone receptor components were not correlated with tissue-specific ecdysone response genes(Fbp1 and Uro), Suggesting that several other factors involved in the complexity of various ecdysone responses in Drosphila melanogaster. But we showed that the transcripts of EcR-B1 isoforms were expressed with higher level than those of EcR-A in the late third instar larvae of ecdysoneless(ecd-1ts) mutation.