http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 김서우(Kim, Seowoo) (검색결과 28 건)
- 무료
- 기관 내 무료
- 유료
-
Streptavidin이 융합된 DR4 항원에 특이적인 single-chain Fv 항체의 개발
김서우,우상욱,김진규,Kim, Seo Woo,Wu, Sangwook,Kim, Jin-Kyoo 한국미생물학회 2018 미생물학회지 Vol.54 No.4
Streptavidin (STR)과 Biotin system은 Biotin의 Streptavidin에 대한 높은 비공유 친화력(non-covalent affinity; $K_D=10^{-14}M$)과 4 Biotin 결합부위를 갖는 Streptavidin의 tetramer 구조로 인해 복수의 항원결합부위 및 복수의 항원특이성을 갖는 항체를 제조할 수 있기 때문에 가장 활발하게 연구되고 있다. 이 system을 활용하기 위해 우리는 Streptomyces avidinii 염색체 DNA로부터 PCR을 통해 Streptavidin (STR) 유전자를 증폭하고 이를 TRAIL (tumor necrosis factor ${\alpha}$ related apoptosis induced ligand) receptor인 death receptor 4 (DR4)에 특이적으로 결합하는 hAY4 single-chain Fv 항체유전자에 융합시켰다. 대장균에서 발현시킨 STR에 융합된 hAY4 ScFv (hAY4-STR) 항체는 가열시킨 SDS-PAGE에서 43 kDa monomer를 나타내었다. 그러나 가열하지 않은 SDS-PAGE와 Size-exclusion chromatography에서는 tetramer인 172 kDa을 나타내었는데 이는 hAY4 ScFv-STR 항체가 STR의 자연적인 비공유결합에 의해 유도된 tetramer를 형성하고 있음을 나타내고 있다. 본 융합 단백질은 Ouchterlony assay와 ELISA에서 보여주는 것처럼 자연 Streptavidin과 유사한 Biotin 결합력을 유지하고 있었다. ELISA와 Westernblot을 이용하여 정제된 hAY4-STR 융합항체의 DR4 항원결합력 또한 확인하였다. 게다가 표면 플라즈몬 공명(surface plasmon resonance) 분석에서 hAY4 ScFv-STR tetramer는 tetramerization에 의해 hAY4 ScFv monomer보다 60배 더 높은 항원결합력을 나타내었다. 요약하면 hAY4 ScFv-STR 융합단백질은 E. coli에서 soluble tetramer로 성공적으로 발현 및 정제되었으며 Biotin과 DR4 항원에 동시에 결합함을 보여 주었다. 이는 bifunctional and tetrameric ScFv 항체를 제조 할 수 있음을 제시해 주고 있다. The Streptavidin and Biotin system has been studied most extensively as the high affinity non-covalent binding of Biotin to STR ($K_D=10^{-14}M$) and four Biotin binding sites in tetrameric Streptavidin makes this system useful for the production of multivalent antibody. For the application of this system, we cloned Streptavidin amplified from Streptomyces avidinii chromosome by PCR and fused to gene of hAY4 single-chain Fv antibody specific to death receptor 4 (DR4) which is a receptor for tumor necrosis factor ${\alpha}$ related apoptosis induced ligand. The hAY4 single-chain Fv antibody fused to Streptavidin expressed in Escherichia coli showed 43 kDa monomer in heated SDS-PAGE. However, this fusion protein shown in both non-heated SDS-PAGE and Size-exclusion chromatography exhibited 172 kDa as a tetramer suggesting that natural tetramerization of Streptavidin by non-covalent association induced hAY4 single-chain Fv tetramerization. This fusion protein retained a Biotin binding activity similar to natural Streptavidin as shown in Ouchterlony assay and ELISA. Death receptor 4 antigen binding activity of purified hAY4 single-chain Fv fused to Streptavidin was also confirmed by ELISA and Westernblot. In addition, surface plasmon resonance analysis showed 60-fold higher antigen binding affinity of the hAY4-STR than monomeric hAY4 ScFv due to tetramerization. In summary, hAY4 single-chain Fv fused to Streptavidin fusion protein was successfully expressed and purified as a soluble tetramer in E. coli and showed both Biotin and DR4 antigen binding activity suggesting possible production of bifunctional and tetrameric ScFv antibody.
-
김서우 ( Seowoo Kim ),남윤자 ( Yun Ja Nam ),김경선 ( Kyoung Sun Kim ) 한국의류학회 2020 한국의류학회지 Vol.44 No.2
In this study, the 4th (1997) and 6th (2013) direct measurements of Size Korea compared the changing shape of women between the ages of 12 and 18. Comparing the items of height and length, the height and shoulder height were significantly reduced, the waist height was not significantly different, and the height of the upper hip was significantly increased, resulting in a smaller upper torso ratio and a higher lower torso rate. The width and thickness associated with human obesity, the circumference items and obesity levels often increased significantly with the change of the times, indicating that the overall body size was increased and that the chest area was changed to a cylindrical shape with changes in the breast equilibrium. Comparing agespecific measurements with graphs analyzing the trend of change in growth, the results showed that the change in 1997 was minimal since age 14; however, a continuous increase was achieved in 2013 that corresponded to the age at which growth is complete. The results of these studies are expected to be used as basic data to predict pattern design, body implementation, and trends in shape changes for young women.
-
CPTED 관점에서 본 공공청사의 현황 및 개선방안 연구
김서우(Seou Kim) 한국셉테드학회 2022 한국셉테드학회지 Vol.13 No.2
본 연구에서는 국가 및 지방정부의 공적인 업무를 수행하는 공공청사가 침입 등의 범죄예방 측면에서 안전한지, 또 빈번한 집회 및 시위로 인한 피해를 최소화할 수 있도록 계획되고 있는지를 확인하고 개선방안 도출하고자 하였다. 연구성과로서 첫째, 정부청사의 경우 2016년 12월에 「정부청사관리 규정 및 시행규칙」에 ‘청사의 출입 관리’ 및 ‘방호 진단’을 신설하였으나 지방 정부청사의 경우 아직 제도적으로 반영되지 못한 상태이다. 또한 여기에 반영된 내용은 「건축법」에 따른 아파트 등에 적용되는 범죄예방 건축기준에 한참 미치지 못하는 수준임을 확인할 수 있었다. 둘째, 공공청사를 대상으로 한 집회 및 시위가 증가하고 있고, 소음 등으로 인해 공공청사에서 업무수행에 어려움을 겪고 있는 것으로 설문조사 결과 분석되었다. 셋째, 공공청사가 범죄로부터 안전하기 위해서는 공공청사에도 「건축법」에 따른 범죄예방 설계 기준이 적용되어야 하며, 시위로 인한 업무의 피해를 최소화하기 위해서는 차음 성능이 강화된 창호 설치 등의 조치가 필요하다. The purpose of this study is to investigate government office buildings and the environmental factors that contribute to crime preventative designs of those buildings. It has been found that: first, in the case of central government buildings, “access management of government buildings” and “protection diagnosis” were newly established in the 「government building management regulations and enforcement regulations」 in December, 2016. However, this change has yet to be established in local government buildings. In addition, it was confirmed that the contents of regulations reflected here were far below par compared to the crime prevention building standards applied to personal apartments under the 「Building Act」. Second, as rallies and protests that take place near government office buildings increase every year, surveys confirm that exposure to noise has a negative impact on work performance. Finally, it is crucial that crime preventative designs should be strictly controlled by regulation (ex. building codes), so that civil servants are protected from crimes. It is necessary to improve sound insulation performance of windows to minimize the noise generated by protests to not hinder the work performance of civil servants. These findings help understand the current environmental conditions of government office buildings associated with crime prevention facilities. There is room for improvement in the principles and process to make the government office building better, safer and more pleasant for both citizens and officials.
-
김서우 ( Seo Woo Kim ),김현경 ( Hyun Kyung Kim ),전승정 ( Sung Joung Jeun ),박혜성 ( Hye Sung Park ),장중현 ( Jung Hyun Jang ),이진화 ( Jin Hwa Lee ),류연주 ( Yon Ju Ryu ),심성신 ( Sung Shin Sim ),천은미 ( Eun Mi Chun ) 대한결핵 및 호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.4
Lymphadenopathy in the thoracic cavity is frequently caused by inflammatory diseases. In very rare cases, the node-bronchial fistula has been reported to be the cause of complications of pulmonary tuberculosis. A male patient with necrotizing pneumonia and mediastinal lymph node enlargements identified by chest computed tomography was also found to have a node-bronchial fistula caused by lung cancer. The patient was treated for tuberculosis with pneumonia for one week before a definitive diagnosis was made. A further investigation revealed him to have non-small cell lung cancer (NSCLC, adenocarcinoma) and multiple mediastinal lymphadenopathies accompanied with the node-bronchial fistula. We report this specific case that had been previously treated for tuberculosis but was later revealed to be NSCLC accompanied with a node-bronchial fistula.
-
-
-
-
저가의 머신 비전 카메라를 이용한 2차원 진동의 측정 및 교정
김서우,이정권,Kim, Seo Woo,Ih, Jeong-Guon 한국음향학회 2018 韓國音響學會誌 Vol.37 No.2
현존하는 진동 측정 센서는 정밀도 면에서는 대부분의 진동을 측정하기에 충분하나, 센서 한 개로 하나의 지점이나 방향에 한정하여 측정할 수밖에 없다는 단점을 갖고 있다. 반면 카메라의 경우, 정밀도나 측정 가능한 주파수 영역의 면에서는 다소 불리하지만, 한 번에 광범위한 영역의 진동을 측정할 수 있고 가격 면에서 유리하며 다자유도의 진동을 동시에 측정할 수 있다는 큰 장점을 갖고 있다. 본 연구에서는 저가의 머신 비전 카메라가 진동 측정 센서로서 어느 정도의 오차 범위 내에서 진동을 측정할 수 있는지 알아본 후, 실제 외팔보의 진동을 측정하였다. 카메라의 2차원 평면 이미지는 두 방향의 직선 운동과 한 방향의 회전 운동을 나타낼 수 있다. 먼저 단일 점의 진동을 카메라로 측정하고, LDV(Laser Doppler Vibrometer) 측정을 기준으로 한 카메라 측정의 오차를 실험적으로 교정하였다. 다음으로 다중점의 진동을 한 번에 측정하여 회전 진동과 외팔보 전체 형상의 진동을 측정하였다. 외팔보 전체 형상 진동은 주파수와 시간 영역 모두에 대하여 분석하였다. The precision of the vibration-sensors, contact or non-contact types, is usually satisfactory for the practical measurement applications, but a sensor is confined to the measurement of a point or a direction. Although the precision and frequency span of the low-cost camera are inferior to these sensors, it has the merits in the cost and in the capability of simultaneous measurement of a large vibrating area. Furthermore, a camera can measure multi-degrees-of-freedom of a vibrating object simultaneously. In this study, the calibration method and the dynamic characteristics of the low-cost machine vision camera as a sensor are studied with a demonstrating example of the two-dimensional vibration of a cantilever beam. The planar image of the camera shot reveals two rectilinear and one rotational motion. The rectilinear vibration motion of a single point is first measured using a camera and the camera is experimentally calibrated by calculating error referencing the LDV (Laser Doppler Vibrometer) measurement. Then, by measuring the motion of multiple points at once, the rotational vibration motion and the whole vibration motion of the cantilever beam are measured. The whole vibration motion of the cantilever beam is analyzed both in time and frequency domain.
-
김한솔(Han-Sol Kim),문우성(Woo-Sung Moon),서우진(Woo-Jin Seo),백광렬(Kwang-Ryul Baek) 제어로봇시스템학회 2013 제어·로봇·시스템학회 논문지 Vol.19 No.4
Geomagnetic is refracted by building’s wall and pillar. Therefore refracted geomagnetic is able to be used as feature point. In a specific space, a mobile device that is equipped with magnetic sensor array measures 3-axis magnetic field for each point. Magnetic field map is acquired by collecting the every sample point in the magnetic field. The measured magnetic field must be calibrated, because each magnetic sensor has a distortion. For this reason, sensor distortion model and sensor calibration method are proposed in this paper. Magnetic field that is measured by mobile device matches magnetic field map. Result of the matching is used for position estimation. This paper implements hardware system for position estimation method using magnetic field map.
-
KISS연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
