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관류 흰쥐 간에서 1,2-Dioctanoyl-sn-Glycerol에 의한 글루코오스의 유리작용
황영은(Young Eun Hwang),문은순(Eun Soon Moon),김미영(Mie Young Kim) 대한약학회 1991 약학회지 Vol.35 No.6
The effect of diacylglycerol on glucose release was studied by using 1,2-dioctanoyl- sn-glycerol (diC8), a cell permeable diacylglycerol, in perfused rat liver. The glucose release was increased by diC8(50 mcM), and the effect was depended on calcium ions. The increment of glucose release by diC8(50 mcM) was inhibited by indomethacin (50 mcM); the amount of glucose release was almost the same with that of control group. Arachidonic acid(200 mcM) also increased glucose release and the release was inhibited by indomethacin. There was no synergistic effect on glucose release by the combination of diC8(50 mcM) and phenylephrine(10 mcM).
흰쥐 hepatocyte에서 알파 및 베타 아드레날린 수용체의 자극에 의한 글리코겐분해에 있어서 칼슘과 니페디핀의 작용
이영희(Young Hee Lee),김준겸(Joon Kyum Kim),김미영(Mie Young Kim) 대한약학회 1988 약학회지 Vol.32 No.6
The effects of calcium and calcium antagonist, nifedipine on the adrenergic receptor- stimulated glycogenolysis were investigated in isolated rat hepatocytes. The hepatic glycogenolysis induced by alpha-adrenergic receptor stimulation depended on calcium ions, and beta-adrenergic activation was unrelated to calcium ions. Nifedipine decreased the alpha-adrenergic agonist-induced glucose release significantly and the decrease was depended on calcium ions. The glucose release induced by beta-adrenergic agonist was not inhibited by nifedipine.
U-937 세포에 있어서 세라마이드에 의한 c-jun 유전자 발현의 조절
김원호,김미영,최경희,Kim, Won-Ho,Kim, Mie-Young,Choi, Kyung-Hee 대한약학회 1997 약학회지 Vol.41 No.1
Ceramide has been suggested as an important mediator of the effects of extracellular agonists on cell growth inhibition, differentiation, apoptosis. However the biochemical sign aling mechanism involved in transducing the effects of ceramide on leukemia cell differentiation is still unclear. In these respects, we examined the regulatory effects of ceramide on c-jun gene expression during differentiation. In U-937 cells. ceramide increased c-jun mRNA levels in a time-dependent manner. The half life, of c-jun mRNA was 30 min. In contrast, inhibition of protein synthesis with cycloheximide in the absence, of transcription with actinomycin D increased the half-life of c-jun mRNA in ceramide-treated U-937 cells to more than 90 min. In order to examine whether ceramide-inhibited c-jun gene expression is regulated through ceramide-activated protein phosphatase (CAPP), a direct target for the action of ceramide, okadaic acid were treated to the cells. Okadaic acid inhibited enhancement of c-jun mRNA induced by C2-ceramide in a dose-dependent manner. These results suggested that ceramide increases c-jun mRNA level during differentiation in U-937 cells and regulates the gene expression on posttranscriptional level. In addition, we provide the evidence that CAPP is involved in ceramide-induced c-jun gene expression in U-937 cells.
선천성 고혈압 쥐에서 고혈압 지속현상과 중추신경계 노르아드레날린성 신경활성과의 상관성
고광호(Kwang Ho Ko),신재수(Jae Soo Shin),김미영(Mie Young Kim) 대한약학회 1986 약학회지 Vol.30 No.6
The relationship between the maintenance of hypertension and the central noradrenergic nervous system activity in spontaneously hypertensive rats (SHR) was studied. The norepinephrine turnover rates in 5 brain areas; telencephalon, hypothalamus/thalamus, midbrain, pons/medulla, cerebellum as a measure of noradrenergic neuronal activity were measured at the ages of 14 weeks in SHR and normotensive Wistar rats. In 14-week old SHR, blood pressure was significantly higher than in normotensive rat, and central norepinephrine turnover rates were significantly greater in telencephalon, hypothalamus/thalamus, midbrain. There were no differences between norepinephrine turnover rates in pons/medulla, cerebellum of SHR and those of normotensive rats.
IM-9 세포에 있어서 세라마이드에 의한 세포주기 변화와 아포프토시스
윤기호(Ki Ho Yoon),최관수(Kwan Soo Choi),김원호(Won Ho Kim),최경희(Kyung Hee Choi),김미영(Mie Young Kim) 대한약학회 1995 약학회지 Vol.39 No.6
Sphingolipids play important roles in cell regulation and signal transduction. Recently, a sphingomyelin cycle has been described in which activation of neutral sphingomyelinase leads to the breakdowm of sphingomyelin and the generation of ceramide. Ceramide, in turn, has emerged as a candidate intracellular mediator for the action of certain cell agonists and has multiple biologic actions. Ceramide is a potent suppressor of cell growth and an inducer of apoptosis. The present studies show that exposure of IM-9 cells to ceramide resulted in internucleosomal cleavage of DNA, yielding laddered patterns of oligonucleosomal fragments characteristic of apoptosis. DNA fragmentation induced by ceramide was also confirmed by diphenylamine assay. The effect of ceramide on cell cycle progression was also studied. The addition of ceramide increased G1 phase distribution in cell cycle, Cell cycle-related cyclin D1 gene expression was decreased in a time-dependent manner. These results suggest that apoptosis induced by ceramide is related to cell cycle associated with the alteration of cell cycle in IM-9 cells.
U-937 세포에서 세라마이드의 세포증식과 세포주기 조절단백질에 대한 작용
이재훈(Jae Hoon Lee),최관수(Kwan Soo Choi),김미영(Mie Young Kim) 대한약학회 1997 약학회지 Vol.41 No.1
Ceramide. a product of sphingomyelin hydrolysis, has been proposed as a lipid second messenger ymediating antiproliferative activation. In this study, we examined the role of the cell cycle-related proteins in the ceramide-mediated growth suppression. Treatment of U-937 cells with C2-ceramide(N-acetylsphingosine) resulted in growth suppression in a time- and concentration dependent manner. Ceramide induced concentration dependent dephosphorylation of retinoblastoma gene product (Rb). Rb remains hypophosphorylated in synchronized cells even after serum stimulation in the presence of ceramide. Ceramide decreased the expression of cyclin D1 and cyclin E levels. These results suggest that antiproliferative effect of ceramide is associated with hypophosphorylation of Rb and decreased expression of cyclin D1 and cyclin E.
적출한 흰쥐 대동맥에 있어서 postsynaptic alpha1-, alpha2- 아드레날린 수용체에 관한 연구
임광진(Guang Jin Im),조윤성(Yun Sung Cho),고광호(Kwang Ho Ko),김미영(Mie Young Kim) 대한약학회 1986 약학회지 Vol.30 No.3
alpha-Adrenoceptor subtypes in the isolated rat aortic strips were examined by using agonists and antagonists which have varying selectivity for alpha1- and alpha2-adrenoceptors. Norepinephrine and phenylephrine produced a similar magnitude of maximum contractions. pA2 values for prazosin and yohimbine were not significantly different using norepinephrine or phenylephrine as the agonist, suggesting a single population of alpha-adrenoceptor. Contractile responses produced by alpha-agonists were antagonized more effectively by prazosin (approximately 1000 fold) than by yohimbine.
최경희,황윤경,김미영 中央大學校 遺傳工學硏究所 1992 遺傳工學硏究論集 Vol.5 No.1
본 연구에서는 PC12 세포에 nicotinic 수용체의 효능제와 저해제를 사용하여 카테콜아민의 방출양상을 조사하고 방출되어 고갈된 카테콜아민을 보충시키기 위한 기작을 조사하기 위하여 카테콜아민 합성효소인 TH mRNA와 TH효소 활성도를 조사하였다. nicotinic 수용체의 효능제인 nicotine을 5분간 처리한 결과 dopamine과 norepinephrine이 대조군에 비해 급격히 방출되었고 이 방출효과는 수용체 저해제인 C6에 의해 저해되었다. 또한 ??M의 nicotine을 PC12 세포에 12, 24, 36, 48시간 처리하고, TH mRNA의 변화양상을 조사한 결과 36시간째 최대치를 보여 대조군의 140% 수준을 나타내었다. 이와같은 변화양상이 nicotine 자극에 의한것인지 확인하기 위하여 C6를 복합처리한 결과 TH mRNA 수준이 거의 대조군수준으로 현저히 감소되었으므로 이들 자극이 nicotinic 수용체를 통해 일어남을 알 수 있었다. 이상의 결과는 nicotine(장시간 처리시)에 의해 부분적으로 고갈된 카테콜아민을 보충시키기 위해서는 TH 유전자 발현이 유도되어 TH효소 단백질의 효소 활성도가 증가됨으로써 이루어짐을 나타내고 있다. The purpose of the present study was to evaluate the functional role of nicotinic receptors in the secretion of catecholamines and to examine the repletion mechanism of depleted catecholamines in rat adrenal pheochromocytoma 12 cells. We investigated, firstly, the secretion pattern of catecholamines after stimulation with nicotine. Secretion of dopamine and norepinephrine was rapidly induced by the treatment with nicotine(1×??M) for 5 mins, and this induction was blocked by the nicotinic receptor antagonist, hexamethonium bromide. Secondly, we examined the tyrosine hydroxylase(TH) activity and TH mRNA level. During the long term incubation(up to 48 h), both TM mRNA and TH activity increased 1.9-fold and 1.4-fold, respectively, at 36 h after the treatment of nicotine. These data suggest that one of the possible mechanisms for the repletion of the depleted catecholamines involves the enhancement of transcriptional control.
6-Exomethylene penamsulfone 유도체의 β-lactamase 저해작용
김해종,여성현,임채욱,임철부,김미영 중앙대학교 약학연구소 1996 약학 논총 Vol.10 No.-
6-Exomethylene penam derivatives were tested as possible β-lactamase inhibitors. The in vitro β-lactamase inhibitory activities were determined by a spectrophotometric assay using Type Ⅰ, Ⅱ, Ⅲ, Ⅳ and TEM β-lactamase. Their activities were compared with sulbactam, tazobactam and clavulanic acid. The 6-exomethylene sulfone derivatives were more potent than clavulanic acid and sulbactam against Type Ⅳ β-lactamase. The Z-isomers showed stronger activities than the E-isomers against TEM and Type Ⅳ β-lactamase.