미세각막절개도를 이용하여 얻은 돼지눈 표층이식편의 두께 및 지름 예측성

김무상,이승찬,이승준,진경현,Moo Sang Kim,Seung Chan Lee,Seung Jun Lee,Kyung Hyun Jin 대한안과학회 2007 대한안과학회지 Vol.48 No.4

다리뇌출혈로 인해 발생한 양방향 수평주시마비 1예

김무상,박인기.Moo-Sang Kim. M.D.. In-Ki Park. M.D. 대한안과학회 2005 대한안과학회지 Vol.46 No.10

Purpose: To report a case of bilateral horizontal gaze palsy associated with pontine hemorrhage. Methods: A 51-year-old man developed a sudden decrease in consciousness. Computed tomography reveal a pontine hemorrhage and infarctions of both basal ganglia. The mentality was recovered after conservative treatment, but he complained of difficulty in moving his eyes. We performed ophthalmic and radiologic evaluations to confirm a diagnosis of bilateral horizontal gaze palsy and to reveal the condition. Results: On ophthalmic examination, his eye showed complete bilateral horizontal gaze palsy, but convergence and vertical eye movements were normal. Radiologic evaluation revealed a pontine hemorrhage, and the hemorrhagic area was consistent the bilateral paramedian pontine reticular formation (PPRF).

양안에 발생한 홍채낭종 1예

김무상,진경현,김동희.Moo-Sang Kim. M.D.. Kyung-Hyun Jin. M.D.. Dong-Hee Kim. M.D. 대한안과학회 2005 대한안과학회지 Vol.46 No.7

Purpose: Iris cyst is a rare condition and there has been no Korean report of bilateral iris cyst. The authors present the first Korean report of a patient with bilateral iris cysts. Methods: A 62-year-old man presented with intermittent visual field disturbance of the left eye when exposed to bright light. The patient underwent visual field examination and slit lamp examination. On slit lamp examination, a dark-brown mass was revealed along the lateral pupillary margin in the left eye. In the right eye, the shape of the iris was convex at the 8 o`clock direction. Ultrasound biomicroscopy, confirmed the presence of a mass. Results: The patient showed no specific abnormality in visual field examination. Ultrasound biomicroscopy showed the objects to be cystic, with a thin-walled homogenous hypoechoic lesion on the posterior surface of the iris in both eyes. There were two cysts in each eye measuring 4.35 mm×1.13 mm and 1.47 mm×1.11 mm in the left eye, and 1.05 mm×1.02 mm and 1.90 mm×1.05 mm in the right eye. Conclusions: Asymptomatic iris cyst does not need to undergo treatment, but careful examination is nevertheless required required at regular intervals in case a complication develops.

An Experimental Study on the Aerodynamic Characteristics around Moving Bodies near the Ground

Moo-Sang Kim(김무상),Tae-Yoon Kim(김태윤),Jung-Do Kee(기정도),Dong-Ho Lee(이동호) 한국자동차공학회 2006 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

The objective of present study is to compare the flow characteristics around moving bodies near the ground. The influence of the gap height and ground conditions, the moving and fixed grounds, on the aerodynamic forces as well as the dynamic behavior of the wake flow around the square cylinder near a ground is investigated. The model wind tunnel experiments were carried out in an open circuit low turbulence subsonic wind tunnel. The wind tunnel is equipped with moving belt system to simulate the ground effect. The mean velocity and the turbulent field around a square cylinder were measured using a two-component Particle Image Velocimetry (PlV). From the velocity profiles in wake region, the pressure recovery on a moving ground decreases due to the high momentum supply, finally causing the drag to increase from the fixed ground, while the lift has opposite pattern. Through the full scale wind tunnel testing, aerodynamic force difference occurs under ground conditions and increases as yaw angle increases. Therefore the adequate ground simulation is necessary to enhance the control stability under the transient crosswind.

시험모델 정밀도가 공력성능 예측에 미치는 영향에 관한 연구

김무상(Moo-Sang Kim),기정도(Jung-Do Kee),오민수(Min-Su Oh) 한국자동차공학회 2004 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

Vehicle aerodynamic performance is developed using both full-scale wind tunnel as well as quarter scale model at Hyundai-Kia Nam_yang R&D Center. The model wind tunnel is a relatively useful tool compared to the full-scale wind tunnel especially when the aerodynamic performance of a new vehicle is being predicted during the styling stage for a new vehicle. Model scale test can provide valuable aerodynamic data well before the prototype or pilot vehicle construction stage if the geometry and Reynolds similarities are considered properly for the scale model. The prediction accuracy of vehicle aerodynamic performance depends on the detailing of the test model such as underbody and engine room geometry.

Cellular Localization and Translocation of Duplication and Alternative Splicing Variants of Olive Flounder Phospholipase C-δ1

Na Young Kim(김나영),Moo-Sang Kim(김무상),Sung Hee Jung(정승희),Myoung Sug Kim(김명석),Mi Young Cho(조미영),Joon Ki Chung(정준기),Sang Jung Ahn(안상중) 한국생명과학회 2017 생명과학회지 Vol.27 No.11

본 연구의 목적은 넙치 인지질가수분해효소(PLC- δ1) 3가지 타입의 세포내 특성을 규명하고자 하였다. 일반적으로 인지질가수분해효소(PLC)의 신호전달경로는 핵, 세포막, 세포질에 분포한다고 알려져 있으나, 핵내 위치 메커니즘은 여전히 불분명하다. PoPLC-δ1A, PoPLC-δ1B (Sf)과 PoPLC-δ1B (Lf)의 3타입의 유전자들은 각각 핵위치 신호(NLS)와 핵방출서열(NES)을 포함하고 있다. 본 연구에서는, 넙치 3가지 타입 인지질가수분해효소(PLC-δ1)의 세포내 위치이동 메커니즘 분석을 위해 GFP 벡터에 유전자를 삽입하여 ionomycin과 thasogargin처리 후 세포위치와 이동양상을 공초점 레이저 주사현미경으로 관찰하였다. PoPLC-δ1A는 PoPLC-δ1B (Lf)와 PoPLC-δ1B (Sf)가 원형질막에 국한되어 분포할때 세포질과 세포막보다 세포 소기관에 분포되어 있었다. PoPLC-δ1B (Lf) 및 PoPLC-δ1 (Sf)이 핵 세포질내 이동양상을 보이지 않을 때, PoPLC-δ1A은 ionomycin과 thapsigargin 처리에 의해 핵 내에 축적되는 양상을 나타냈다. 이런 결과는 손상되지 않은 기능적 NES 서열을 포함하는 PoPLC-δ1A가 어류에서 핵 세포질 내 왕복 및 이동의 주된 역할을 한다는 것을 보여주고 있다. The purpose of this study was to investigate the cellular characterization of phospholipase C-δ1 in olive flounders (Paralichthys olivaceus). In general, phospholipase C signaling pathways are distributed in nuclei at plasma membranes and in cytoplasms, although the pathways" nuclear localization mechanisms are unclear. P. olivaceus duplicates type-A PoPLC-δ1 (PoPLC-δ1A), which has a high similarity to the human isoform PLC-δ; type-B PoPLC-δ1 (PoPLC-δ1B [Sf]), which has a low similarity to the human isoform PLC-δ and the alternative splice variant PoPLC-δ1B (Lf), which has a nuclear localization signal (NLS) and a nuclear export signal (NES) for nuclear imports and exports, respectively. This study confirmed the effects of the cellular localization and translocation of GFP-tagged PoPLC-δ1A, PoPLC-δ1B (Sf) and PoPLC-δ1B (Lf). It administered treatments of Ca<SUP>2+</SUP> ionophore ionomycin and endoplasmic reticulum (ER)–Ca2+ pump inhibitor thapsigargin to hirame natural-embryo (HINAE) cells. A laser-scanning confocal microscope was used. GFP-tagged PoPLC-δ1A was distributed to the cellular organelles, rather than to the cytoplasms and cytomembranes, when PoPLC-δ1B (Lf) and PoPLC-δ1B (Sf) were localized at the plasma membranes. The treatments of ionomycin and thapsigargin showed the accumulation of PoPLC-δ1A in the nuclei when PoPLC-δ1B (Lf) nucleocytoplasmic shuttling and PoPLC-δ1B (Sf) nucleocytoplasmic shuttling were not observed. The results were the first evidence that PoPLC-δ1A, which contains functional, intact NES sequences, has a main role in nucleocytoplasmic shuttling and translocation in fish.

서울지역 급성위장관염 환자에서 검출된 노로바이러스의 유전자형 분포

김은정,박상훈,송미옥,김무상,김민영,천두성,정혜숙,김철중,Kim, Eun-Jeung,Park, Sang-Hun,Song, Mi-Ok,Kim, Moo-Sang,Kim, Min-Young,Cheon, Doo-Sung,Jeong, Hae-Sook,Kim, Chul-Joong 한국미생물학회 2008 미생물학회지 Vol.44 No.2

2004년부터 207년까지 서울지역에서 산발적으로 발생한 급성위장관염 환자에 대하여 RT-PCR을 이용하여 노로바이러스를 검출하였다. 검출된 노로바이러스 258건에 대하여 염기서열분석을 실시한 결과 GII.1, GII.2, GII.3, GII.4, GII.5, GII.6, GII.8, GII.10, GII.12, GII.13, GII.15, GII.16, GII.17 등 13가지 유전자형이 검출되었으며 산발적으로 발생한 급성위장관염 환자에서 가장 많이 검출된 유전자형은 GII.4(76.7%)로 나타났다. 노로바이러스 유전자형의 다양성에 대한 연구로 서울지역 노로바이러스성 질환의 예방을 위한 기초자료로 활용될 것으로 기대된다. Fecal specimens from acute gastroenteritis in Seoul from 2004 to 2007 were collected and then tested for the presence of norovirus by RT-PCR. 258 noroviruses were subjected to be futher characterized to sequencing analysis. The sequencing analysis showed that thirteen genotypes were detected (GII.1, GII.2, GII.3, GII.4, GII.5, GII.6, GII.8, GII.10, GII.l2, GII.13, GII.l5, GII.l6, GII.l7) and predominant genotype was GII.4 (76.7%) in the cases of norovirus detected sporadic acute gastroenteritis in Seoul. By this molecular investigation, genotypic distribution associated with norovirus infections will be used for control and prevention of norovirus related diseases.

횡풍안정성 평가기법의 개발

박상현(Sang-Hyun Park),김석래(Seok Lae Kim),이강덕(Kang Duck Ih),김무상(Moo Sang Kim) 한국자동차공학회 2010 한국자동차공학회 부문종합 학술대회 Vol.2010 No.5

Crosswind is an important factor that affects driving safety on expressways. Highways in certain areas have seasonal or year-round crosswind strong enough to cause surged traffic accidents. In addition, vehicles driving on long span bridges and bridges with high piers could experience structure induced winds that pose severe threats. Therefore, investigating the crosswind behavior of passenger cars has been an important issue for vehicle engineers. Crosswind stability of a car can be examined by crosswind-generator tests or on-road driving tests in a natural stochastic crosswind. In this paper, the application of the crosswind stability test with a virtual vehicle and a virtual driver as well as the on-road driving test with were implemented. From the frequency domain results of the crosswind measurements the driver’s subjective assessment was derived and objective assessment was calculated. The good evaluation of the compensation efficiency in spite of the large overall vehicle reactions reflects the subjective assessment of the test drivers during the crosswind measurements.

서울지역 급성위장관염 환자 및 지하수에서 분리한 A형 로타바이러스의 유전자형

김은정,김무상,채영주,천두성,Kim, Eun-Jeung,Kim, Moo-Sang,Chae, Young-Zoo,Cheon, Doo-Sung 한국미생물학회 2011 미생물학회지 Vol.47 No.4

2009년부터 2010년까지 서울지역에서 산발적으로 발생한 급성위장관염 환자에 대하여 효소면역측정법(ELISA)을 이용하여 로타바이러스를 검출하였다. 총 1,916건 중 로타바이러스는 354건(18.4%)이 검출되었다. RT-PCR을 이용하여 유전자형 분석을 실시한 결과 P6G[4] (35%), P8G[3] (28%), P8G[1] (24%), P4G[2] (10%), P8G[9] (3%)로 나타났다. 서울지역 지하수 70지점을 대상으로 효소면역측정법을 이용하여 로타바이러스를 조사한 결과 2 지점에서 양성으로 검출되었으며, RT-PCR을 이용하여 유전자형 분석을 실시한 결과 P8G[3]로 나타났다. 이러한 연구결과는 서울지역 로타바이러스 질환의 예방을 위한 기초자료로 활용될 것으로 기대된다. Fecal specimens from acute gastroenteritis in Seoul from 2009 to 2010 were collected and then tested for the presence of Group A Rotavirus by ELISA. Among a total of 1,916 samples investigated, 354 samples (18.4%) were positive. The predominant genotypes of positive samples were confirmed as P6G[4] (35%), P8G[3] (28%), P8G[1] (24%), P4G[2] (10%), P8G[9] (3%), respectively. Among a total of 70 ground water samples investigated, 2 samples (2.8%) were positive. The genotypes of positive samples were confirmed as P8G[3] (100%). By this molecular investigation, genotypic distribution associated with rotavirus will be used for control and prevention of rotavirus related diseases.

서울지역 집단식중독 환자에서 검출된 노로바이러스의 유행양상

김은정,김무상,채영주,천두성,Kim, Eun-Jeung,Kim, Moo-Sang,Chae, Young-Zoo,Cheon, Doo-Sung 한국미생물학회 2012 미생물학회지 Vol.48 No.2

Fecal specimens from acute gastroenteritis in Seoul from 2003 to 2007 were collected and then tested for the presence of Norovirus by RT-PCR. Among a total of 4,685 samples investigated, 383 samples (8.2%) were positive. The analysis of outbreaks related norovirus contamination occurred from 2003 to 2007 in Seoul revealed 57 cases happened during investigated period. Seasonal prevalence showed winter season predominant characteristics of pattern of epidemics by long term investigation for norovirus infections. The incidence of norovirus infection in the case of acute gastroenteritis by catering food in school were 32%, by food in general restaurant were 29%. This epidemiological investigation in Seoul was strongly needed for control and prevention of outbreaks related with norovirus by forecasting disease epidemics. 2003년부터 2007년까지 서울지역 집단식중독 환자에 대하여 RT-PCR을 이용하여 노로바이러스를 검출하였으며, 총 4,685건 중 노로바이러스가 383건(8.2%)이 검출되었다. 식중독 발생 사례는 2003년부터 2007년까지 57사례가 발생하였다. 계절별 유행양상은 겨울철에 가장 많이 검출되었다. 섭취장소별 유행양상은 학교급식 중 위탁경영에 의한 발생이 32%로 나타났으며, 일반음식점에 의한 발생이 29%로 나타났다. 본 연구는 서울지역 노로바이러스성 식중독 관리를 위한 기초자료로 활용될 수 있을 것으로 기대된다.