곰팡이 균사체 기반 복합소재의 연구 동향과 제품 개발 가능성

김다송(Da-Song Kim),김용운(Yong-Woon Kim),김길자(Kil-Ja Kim),신현재(Hyun-Jae Shin) 한국생물공학회 2017 KSBB Journal Vol.32 No.3

Fungal mycelium-based composite materials (FMBC) are a new biomaterial to replace the existing composite materials. To compete with lightweight, high-performance composite materials represented by fiber-reinforced plastic (FRP), various physical and chemical properties and functionality must be secured. Especially, the composite materials made by using mycelium of mushroom is called mushroom plastic. Currently, Ecovative, Grado Zero Espace and MycoWorks in USA and Europe are launching new products. Products utilizing FMBC can be launched in the market for construction materials, automobile interior materials and artificial leather substitutes. In spite of this high possibility, mass production using FMBC has not yet been reported. This review introduces the FMBC, a material that can replace existing plastics, inorganic building materials and animal skins in an environmentally and economically viable way, and looks at the possibility of future biomaterials by summarizing recent research contents.

전처리 방법이 감압후라이드 고구마칩의 물리적 특성에 미치는 영향

김다송 ( Da-song Kim ),신현재 ( Hyun-jae Shin ) 조선대학교 공학기술연구원 2021 공학기술논문지 Vol.14 No.2

Recently, the demand for healthy snacks from domestic agricultural products is increasing with the recent well-being trends. For vacuum frying, the frying temperature and oxygen concentration are low, so the oxidation reactions of oil are significantly reduced compared to deep frying. Therefore, vacuum-fried products are suitable for healthy food manufacturing due to their low content of harmful ingredients such as trans fats, polar compounds, and acrylamide. The vacuum-fried chips show different physical properties compared to deep-fried one with pretreatment methods, and product quality of the snack is strongly dependent on physical properties. In this study, sweet potatoes and purple sweet potatoes were investigated with various pretreatments to measure their physical properties. The pretreatment methods used were predrying, blanching, osmotic dehydration, antibrowning agent and hydrocolloid coating. The physical properties of vacuum-fried chips were measured for yield, color, oil content, and hardness. As a result, changes in physical properties were evident according to the pretreatment method. Product yield and oil content are important factors for producers and consumers, and considering this, the best pretreatment method was osmotic dehydration. The results could be used to produce premium-grade sweet potatoes and purple sweet potatoes chips based agricultural products with high palatability and low acrylamide contents.

Bacillus subtilis를 이용한 국내산 신이대 잎 발효에 따른 영양성분, 폴리페놀, 항산화능 변화

조한교(Han-Gyo Jo),김다송(Da-Song Kim),신현재(Hyun-Jae Shin) 한국생물공학회 2017 KSBB Journal Vol.32 No.1

Bamboo leaf has many nutritional and bioactive compounds such as polyphenols, and it can be used for various foods application. Fermentation is one of appropriate processes that can affect the nutrition, taste and flavor, and antioxidant activities of foods. In this study, a representative domestic bamboo tree (Sasa coreana Nakai) leaves were fermented by Bacillus subtilis KCCM 11315 and the changes of carbohydrates, minerals, amino acids, and phenolic compounds and antioxidant activities were investigated before and after fermentation. During the fermentation period, firstly, the leaf was softened and turned to brown. The constituent carbohydrates were slightly increased from 432.09±5.38 mg/g to 458.42±7.39 mg/g, and free sugars decreased by 95% from 28.12±2.03 mg/g to 1.4±0.14 mg/g. Mineral was 20987.5±345.1 μg/mL, which was slightly increased after the fermentation compared to 20804.1±364.6 μg/mL before that. The total amino acids were increased to 73881.94±137.59 mg/100 g compared to 58464.51±109.12 mg/100 g before fermentation, and free amino acids decreased by more than 85% from 32782.67±92.49 mg/100 g to 4827.83±19.76 mg/100 g. Total polyphenols content (TPC) increased from 25.51±1.04 GAE*mg/g to 35.34±0.91 GAE*mg/g, and the total flavonoid content (TFC) increased to 80.57±0.22 QE*mg/g compared to 69.64±0.26 QE<SUP>*</SUP>mg/g. The total catechin content (TCC) of TFC was decreased from 69.64±0.94 mg/L to 58.23±0.76 mg/L. The DPPH radical IC50 of bamboo leaves decreased to 2624.85±37.03 μg/mL and the ABTS radical IC50 of 187.26±4.78 μg/mL was increased after the fermentation. These results could be used as essential nutritional data before developing processed food products using the bamboo leaf.

국내산 대나무 줄기에서 추출한 실리카의 주름개선 효과

최문희(Moon-Hee Choi),김다송(Da-Song Kim),신현재(Hyun-Jae Shin) 한국생물공학회 2017 KSBB Journal Vol.32 No.4

Bamboo stems extract containing porous silica has been used for medicine, food, paper and craft industry. Bamboo silica is known to support absorbing essential minerals such as calcium, potassium, and magnesium in skin, nail and hair. In this study, extract of domestic bamboos containing silica from two species of Phyllostachys nigra var. Enonis Stapf. and Phyllostachys bambusoides was prepared to prepare the bamboo silica materials. Physicochemical properties have been determined with XRD, FT-IR, FE-SEM and EDX. In addition, enzyme inhibition assay of collagenase and elastase has been performed. Phyllostachys nigra var. Enonis Stapf. silica (PNS) and Phyllostachys bambusoides silica (PBS) inhibited collagenase with IC50 value of 174.14 μg/mL and 292.60 μg/mL, respectively. While, positive control groups of retinol and α-tocopherol were shown to be IC50 values of 49.43 μg/ mL and 63.70 μg/mL, respectively. Furthermore, PNS and PBS elastase inhibition rate was 179.46 μg/mL and 377.03 μg/mL. These results suggest that PNS and PBS could be used as a candidate for anti-wrinkle new cosmetic ingredients.

제주도 손바닥선인장 추출물의 표면소독제로써의 항균효과

권영란(Kwon Young-ran),김다송(Kim Da-Song),신현재(Shin Hyun-Jae) 한국기초간호학회 2021 Journal of korean biological nursing science Vol.23 No.1

Purpose: To verify the anti-microbiotic activity and to develop eco-friendly surface disinfectants using the Opuntia ficus-indica extract. Methods: The stem and fruits of the Opuntia cultivated in Jeju Island were extracted with hot water, 70% EtOH and 100% MeOH, respectively. To examine the antimicrobial activity of the extracts, gram positive bacteria (Bacillus subtilis, Staphylococcus aureus), gram negative bacteria (Escherichia coli, Salmonella typhimurium), yeast (Candida albicans), mold (Aspergillus flavus) and antibiotic resistant bacteria (E. coli, S. aureus) were measured to form a clear zone. Results: The antibacterial activity of the fruit extract was higher than that of the stem extract, and the antibacterial activity of the 100% MeOH (FM) extract and the 70% EtOH (FE) extract of the palm cactus fruit was suitable for the surface disinfectant overall, and clear-zone formed even at relatively low concentrations (250 mg/mL). The FM and FE have antibacterial activity against various bacteria, therefore they can be used as disinfectants. Conclusion: The antimicrobial activity of Opuntia ficus-indica extract from Jeju Island by stem and fruit was determined at relatively low concentrations (250 mg/mL). The Opuntia ficus-indica extract will be useful for surface disinfectants dissolving the patients’ bath, oral gargling, and powdered powder in water or ethanol in appropriate concentrations.

한국 성인의 항산화 식품 섭취에 따른 인체 내 항산화능 변화를 예측하는 비파괴적 공명 라만분광법

최문희 ( Moon-hee Choi ),조한교 ( Han-gyo Jo ),김다송 ( Da-song Kim ),신현재 ( Hyun-jae Shin ) 조선대학교 공학기술연구원 2017 공학기술논문지 Vol.10 No.1

Aging is a phenomenon caused by a decrease in antioxidant activity, and skin carotenoid could be an appropriate indicator of human antioxidant level. Although many studies have found a strong correlation between serum carotenoids and skin carotenoids measured by resonance Raman spectoscopy (RRS), some information about Korean adult has not been reported yet. To determine if skin carotenoids score (SCS) measured by RRS could be used as a valid marker of antioxidant level among 42 participants, healthy adults aged 20 to 55 were administerd two antioxidant foods (commercial Tablet and wheat sprout powder). RRS equipment (BioPhotonic Scanner, Pharmanex, USA) was used for detecting the skin carotenoid level of the subjects. Daily adminstration of commercial Tablet, wheat sprout powder, and placebo was done for 4 weeks. The measurement of SCS assessed 3 times in each measurement during the period. The results of SCS from 0 day, at least 7 days apart, were statistically analyzed and compared to each group. We cannot find any correlation among three groups. In Tablet group, however, there was correlation with intake time; SCS before intake and SCS at 4 weeks after intake (p<0.05). In antioxidant activity of foods, IC₅₀ of the Tablet is 3 times lower than that of wheat sprout. Commercial Tablet is better than wheat sprout in terms of increase of the SCS and antioxidant level in skin. This result might came from the effect of multiple antioxidant compounds, vitamins, and minerals contained in Tablet. In summary, RRS can determine the in vivo carotenoids concentration and antioxidant level in skin of Korean adult.