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- 저자 : 김낙인 ( Ji Ho Ryou (검색결과 2 건)
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류지호,이주희,김낙인 ( Ji Ho Ryou,Ju Hie Lee,Nack In Kim ) 대한피부과학회 1997 大韓皮膚科學會誌 Vol.35 No.6
Background: Glomus tumor is a benign lesion composed of vessels and glomocytes in varying proportions. Although the histc)logic features of glomus tumors by light microscopy are characteristic and well recognized, there is controversy as to the histogenesis and cytological characterization of the glomus cell. Objective : The aim of the present study was to verify the histopathological origin of a glornus tumor. Method: We investigated 1 cases of glomus tumors for immunohistochemical features. Results : The glomus turnor cells were negative when stained for FactorVIII - related antigen and S-100 protein. Conversely, all materials were found to be positive for actin, vimentin, NSE. Sorne exhibited an equivocal reaction for desmin and CD34, the rest were negative. Conclusion : These findings support the hypothesis that the glomus cell is transitional-between smooth mu.cle and vascular ndothelium-being essentially a modified smooth muscle cell with some endot.helial cell properties (KoreaA J D8rmatOI 1997;35(6): 1130-1135)
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배양 인체 각질형성세포에서 Nerve Growth Factor ( NGF ) 수용체 발현과 NGF 가 세포 증식에 미치는 영향에 관한 연구
박재경,김낙인,류지호,임덕우 대한피부과학회 2001 대한피부과학회지 Vol.39 No.2
Background: Nerve growth factor (NGF) is a neurotrophic polypeptide necessary for the survival and growth of some central neurons, as well as sensory afferent and sympathetic neurons. In addition to its actions on the nervous system, it also has a significant biologic effects on cells of the immune-inflammatory compartment. Recent studies suggest that NGF is an important autocrine growth factor and survival factor for keratinocytes which express both high- and low-affinity receptors for NGF. Objective: The purpose of this study is to detect NFG receptors an cultured human keratinocytes and to evaluate the effect of NGF on proliferation of cultured human keratinocytes. Methods: Cultured human keratinocytes were examined for the expression of high affinity receptor TrkA and low affinity receptor p75 by Northern blot, Western blot and immunocytochemistry, The effects of NGF on proliferation of cultured human keratinocytes were also evaluated. To specify the NGF effect on proliferation of human keratinocytes, excess of anti-NGF neutralizing polyclonal antibody was added. Results: 1) NGF significantly stimulated the proliferation of keratinocytes in both 1% of keratinocyte growth supplement (KGS)-added medium (100ng/ml) and 0.2% KGS-added media(50, 100, 500ng/ml), (p$lt;0.05). The cell number was dose-dependently increased in 0.2% KGS-added media. 2) Whenever we added 500 ng/ml of anti-NGF polyclonal antibody to the growth media, the cell number was statistically higher in 100ng/ml NGF-added group of 1% KGS-added medium, but there was not any statistical significance in 0.2% KGS-added media group. 3) Immunocytochemical staining with specific antibodies to TrkA and p75 revealed positive findings for these receptors, but TrkB and TrkC were not detected. 4) We could not detect both the mRNA and protein of TrkA and p75 by Northern and Western blot methods. Conclusion: These results suggest that both high affinity- and low affinity receptors for NGF are expressed in cultured human keratinocytes and NGF can induce keratinocyte proliferation.
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