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Trichoclerma sp가 生産하는 Cellulase에 關한 硏究
具本聖,金敎昌,南相烈 충북대학교 농업과학기술연구소 1987 農業科學硏究 Vol.5 No.1
This experiment was conducted to investigate the composition of the medium for the cellulase production by Trichoclerma sp. and the characteristics of the cellulase. The results obtained were as follows; 1.The optimum composition of the medium for cellulase production consists of 1% substrate, 0.5% KH2PO4, 0.3% (NH2)2CO, 0.3% NaNO3 0.05% MaSO4·7H2O, 0.01% Fe SO4·7H2O, respectively. 2.The optimum pH of cellulase was 5.0 and pH range of its stability was 4-6. 3.The optimum temperature of cellulase was 50℃ and heat stability was below 50℃. 4.In the concentration of substrate for enzyme, the activities of cellulase were respectively increased to the concentration of 1% substrate, but above the concentration of 3% stbstrate the activities of cellulase were slowly increased. 5.In general the activities of cellulase were proportionally increased by adding the amount of enzyme solution up to 1 ml, but those of cellulase were slowly increased up to 3ml. 6.The cellulase was inhibited by Hg.++