마황(麻黃)과 석고(石膏) 혼합물이 지방전구세포 분화와 흰쥐의 비만에 미치는 영향

기영범 ( Young Bum Kee ),김대훈 ( Dae Hun Kim ),양미성 ( Mi Sung Yang ),강대희 ( Dae Hee Kang ),김선종 ( Sun Jong Kim ),최진봉 ( Jin Bong Choi ) 한방재활의학과학회 2014 한방재활의학과학회지 Vol.24 No.3

Objectives This study was designed to evaluate the effects of Ephedra Sinica and Fibrosum Gypsum extract on obesity by using 3T3-L1 cells and high fat diet rats. Methods In vitro, Ephedra Sinica and Fibrosum Gypsum extract (50, 100, 200, 500 μg/ ml) were added in 3T3-L1 cells. Cytotoxicity was measured by MTT assasy. Adipocyte differentiation was measured by Oil Red O staining, GPDH activity and C/EBPα protein expression. In vivo, Sprague-Dawley rats were divided into 5 groups : Normal diet group (Normal group), taken high fat diet and no treatment group (Control group), taken high fat diet and orally administered Ephedra Sinica and Fibrosum Gypsum extract daily (Group I: 50 mg/kg, Group II: 100 mg/kg, Group III : 200 mg/kg, oral). For 6 weeks of administration, body weight and the amount of food intake were measured once a week. After administration, blood analysis (AST, ALT, T-Bilirubin, BUN, RBC, Hb, HCT), serum lipid level (triglyceride, Total cholesterol, HDL, LDL), serum leptin level, epididymal adipose tissue weight and histological finding of liver were estimated. Results In vitro, The cytotoxicity was not significant. 3T3-L1 cell’s differentiation was significantly decreased in Oil Red O staining, GPDH activity and C/EBPα protein expression. In vivo, Body weight and the amount of food intake, AST, ALT, Total cholesterol, TG, LDL, serum leptin, epididymal adipose tissue weight showed significant decrease in group I, group II and group III. There were no significant difference in T-bilirubin, BUN, RBC, Hb and HCT between all groups. HDL showed significant increase in group I, group II and group III. In histological finding of liver tissue, there were decreased adiposity and cytopathic effect in group I, group II and group III. Conclusions It is suggested that Ephedra Sinica and Fibrosum Gypsum extract can be used in the treatment of obesity. (J Korean Med Rehab 2014;24(3):11-27)

족관절 염좌의 한방치료 효과에 대한 국내 임상논문 고찰

기영범(Young Bum Kee),이주희(Ju Hee Lee) 대한스포츠한의학회 2012 대한스포츠한의학회지 Vol.12 No.1

Objectives This study was planned to analyze Korean medical treatments of ankle sprain through the paper review. Methods We reviewed the 22 clinical trial papers concerned with ankle sprain published in Korea. We searched the clinical trial papers with keywords of ankle sprain(by Korean & English) in the search site, Koreanstudies Information Service System, Oriental Medicine Advanced Searching Integrated System. Results & Conclusions There were 22 clinical studies concerned with ankle sprain. 16 articles were acute ankle sprain studies, 1 article was chronic ankle sprain study, 2 articles were acute, subacute and chronic ankle sprain studies. And there was no classification about acute or chronic ankle sprain in 3 articles. The subjects of clinical trials were Bee Venom Acupuncture, Heating-Conduction Acupuncture, Acupotomy, Moxibustion Treatment, Blood-letting Cupping Treatment, Three-edged needle, Traction Therapy, Positional Release Therapy, Auricular Acupuncture, Meridian tendino-musculature taping, Dong-si Acupuncture, Acupuncture at unaffected limb, Remote acupuncture points, Acupuncture on Peroneus muscle with deep or superficial insertion. 17 articles assessed VAS(Visual Analogue Scale), 2 articles assessed NRS(Numerical Rating Scale), 8 articles assessed AHS (Ankle-Hindfoot Scale), 4 articles assessed pain threshold, 4 articles assessed ROM(Range of motion). Continual study and persistent research are needed to prove therapeutic effect of various Korean medicine treatment.

육미지황탕과 녹용약침이 당뇨유발 흰쥐의 골절치유에 미치는 영향

기영범 ( Young Bum Kee ),김대훈 ( Dae Hun Kim ),강대희 ( Dae Hee Kang ),김선종 ( Sun Jong Kim ),최진봉 ( Jin Bong Choi ) 한방재활의학과학회 2012 한방재활의학과학회지 Vol.22 No.3

Objectives :This study was designed to evaluate the effects of Yukmijihwang-tang(Liuweidihuang-tang) and Cervi Pantotrichum Cornu pharmacopuncture on fracture healing in diabetic rats. Methods :In this study, 80 Sprague-Dawley rats were induced by STZ(streptozotocin) which was in general use as diabetes model. After the subjects were induced femur fracture, we divided the subjects into 4 groups : Control group with no treatment, experimental Ⅰ taken with Yukmijihwang-tang(Liuweidihuang-tang), experimental Ⅱ taken with Cervi Pantotrichum Cornu pharmacopuncture and experimental Ⅲ taken with Yukmijihwang-tang(Liuweidihuang-tang) and Cervi Pantotrichum Cornu pharmacopuncture groups. Serum T-ALP(total alkaline phosphatase), histological observation of callus formation, and the degree of TGF-β1(transforming growth factor beta 1) in each group were observed at the 7th, 14th, 21th day and at the 28th day respectively. Results :1. Experimental group Ⅰ and experimental group Ⅲ showed significant changes in serum T-ALP after 7-days of experimental period. All the experimental groups showed significant changes in serum T-ALP after 14-days of experimental period, especially experimental group Ⅲ showed more significant changes than other groups. 2. After 14-days, 21-days and 28-days, all the experimental groups showed significant changes in histological observation of callus formation, compared with control group. Especially experimental group Ⅲ showed more significant changes than other groups. 3. All the experimental groups showed significant changes in TGF-β1 compared with control group. Especially experimental group Ⅲ showed more significant changes than other groups. 4. Injury of liver tissue and kidney tissue in histological findings were not seen after 28-days of experimental period. Conclusions :It is suggested that Yukmijihwang-tang(Liuweidihuang-tang) and Cervi Pantotrichum Cornu pharmacopuncture may play a significant role in fracture healing in diabetic rats, and these two treatments could be more effective when used together.

비복근 파열(Tennis Leg)에 대한 고찰

이주희(Ju Hee Lee),기영범(Young Bum Kee) 대한스포츠한의학회 2012 대한스포츠한의학회지 Vol.12 No.1

Rupture of the distal musculotendinous junction of the medial head of the gastrocnemius, also called “Tennis Leg”, is a relatively common clinical condition. As the number of people enjoying exercise is increasing, due to the growing concern for healthy life-style, there is a trend more people are suffering from health injuries. One of these sports injuries about the pathogenesis of calf strain, diagnosis, and treatment is for literature.

학술포스터발표회 : 지리산국립공원 피아골과 대성골 지역의 산림구조 동태(1)

오구균,김용식,오장근,기영범 한국환경생태학회 2007 한국환경생태학회 학술대회지 Vol.2007 No.2

족삼리, 양릉천, 현종과 협척혈이 척수 압좌 손상 모델의 신경 재생에 미치는 영향

양미성 ( Mi Sung Yang ),기영범 ( M Sung Yang ),강대희 ( Young Bum Kee ),신미숙 ( Mi Suk Shin ),김선종 ( Sun Jong Kim ),최진봉 ( Jin Bong Choi ) 한방재활의학과학회 2012 한방재활의학과학회지 Vol.22 No.4

Objectives : The purpose of this study was to investigate the effects of ST36, GB34 and GB39, Hyeopcheok points, electrical acupuncture and acupuncture on nerve regeneration and neuromuscular synaptic reorganization in rat model of spinal cord injury(SCI). Methods : The subjects were divided into 5 groups; control group with no treatment after inducing SCI, experimental group Ⅰ taken with acupuncture of Hyeopcheok points after inducing SCI, experimental group Ⅱ taken with acupuncture of ST36, GB34 and GB39 after inducing SCI, experimental group Ⅲ taken with electrical acupuncture of Hyeopcheok points after inducing SCI and experimental group Ⅳ taken with electrical acupuncture of ST36, GB34 and GB39 after inducing SCI. After each operation, the present author observed cytological changes, the motor behavior recovery and nerve regeneration by analysis of the motor behavior tests, histological and immunological changes. Rats were tested at modified Tarlov test at the 1st day, Basso, Beattle, Bresnahan locomotor rating scale(BBB scale) at the 3rd, 7th, 14th and 21 days. Results : 1. Experimental group Ⅳ were improved compared with control group, experimental group Ⅰ, Ⅱ, Ⅲ in the motor behavior test(BBB scale). 2. BDNF(brain-derived neurotrophin factor) expression degree in the SCI tissue of all the experimenral groups were increased compared with control group. Especially, those of experimental group Ⅳ was more effective. 3. Muscular atrophy in gastrocnemius were restored only in experimental group Ⅳ. 4. NT-3(neurotrophin-3) expression degree in the SCI inducing rats of all the experimenral groups were increased compared with control group. Especially, those of experimental group Ⅳ was more effective. Conclusions : It can be suggested that electrical acupuncture of ST36, GB34 and GB39 may improve motor behavior, histological and immunological findings in SCI-induced rats compared with acupuncture of ST36, GB34 and GB39, acupuncture or electrical acupuncture of Hyeopcheok points.

지리산국립공원 피아골과 대성골 지역의 산림군집구조 동태(1)

오구균 ( Koo Kyoon Oh ),김용식 ( Yong Shik Kim ),오장근 ( Jang Guen Oh ),기영범 ( Young Bum Ki ) 한국환경생태학회 2008 한국환경생태학회지 Vol.22 No.5

지리산국립공원 피아골과 대성골 지역의 산림군집구조의 동태를 장기간 모니터링하기 위하여 2001년에 고정조사구를 설치하여 2001년과 2006년에 식생조사를 한 결과는 다음과 같다. 피아골, 대성골 지역 산림의 우점종은 각각 서어나무와 신갈나무였으며, 피아골 지역의 산림이 대성골 지역보다 종다양도 지수가 더 높았다. 조사지역 주요수종의 직경급 분포로 보아 안정된 식물군집 구조를 이루고 있으며, 곡간부에 위치한 피아골의 산림과 소능선 사면부에 위치한 대성골의 산림간 종구성은 매우 이질적이었다. 피아골지역 산림의 생장량은 지난 5년간 약 6.4㎡/ha 감소하였으나 대성골지역은 약 8.27㎡/ha 증가하였으며, 이는 노거수들의 고사에 따른 영향으로 보인다. The permanent monitoring plots were installed in 2001 for long-term monitoring the structure of forest communities at the Piagol(Valley) and Daeseonggol(Valley) in the Jirisan National Park, and monitored the forest structure in the studied sites in 2001 and 2006. Dominant species at Piagol and Daeseonggol was Carpinuslaxiflora and Quercus mongolica respectively. Based on the distribution of major species` stem diameter, the species diversity index was higher in Piagol than that of Daeseonggol. The distribution of diameter in major tree species in the studied sites showed a stable plant community structure. The forest of Piagol, which is positioned in the valley, showed a quite a different composition of species from that of Daeseonggol, which is positioned on the slope. In the last five years, the overall grows rate of Piagol Forest decreased by 6.4㎡ per hectare, while Daeseonggol Forest increased by about 8.27㎡ per hectare. I think that this is because of the fact that a lot of large old trees have died.

교통사고 환자의 어혈변증과 계지복령환(桂枝茯笭丸) 효과의 임상적 연구

조희근 ( Hee Guen Jo ),박애련 ( Ae Ryeon Park ),기영범 ( Young Bum Kee ),강대희 ( Dae Hee Kang ),최진봉 ( Jin Bong Choi ),설재욱 ( Jae Uk Sul ) 한방재활의학과학회 2011 한방재활의학과학회지 Vol.21 No.3

Objectives: The main purpose of this study is to investigate the efficacy of Kyejibokryong-whan(Guizhifuling-wan) and examine the blood stasis for patients with motor vehicle accident. Methods: Of the patients who have visited Dong-Shin university oriental hospital for general pain due to motor vehicle accident from June 2010 to November 2010, we enrolled 29 patients and performed blood stasis diagnosis. Patients were divided into groups according to blood stasis findings. Patients who had more than twenty blood stasis diagnosis point were classified as blood stasis group. Patients who had under twenty blood stasis diagnosis point were classified as non blood stasis group. We administered Kyejibokryong-whan(Guizhifuling-wan) to all group. The effect was assessed by VAS(visual analogue scale), SF-MPQ(short form-McGill pain questionnaire) ODI(Oswestry disability index) & NDI(neck disability index). Results: The pain & function index decreased in both groups after treatment. Conclusions: The difference between blood stasis group and non blood stasis group were not significant at after treatment stage. This data suggested that the method of classifying blood stasis is useful and the Kyejibokryong-whan(Guizhifuling-wan) can be effective means that decrease pain caused by motor vehicle accident.

요추 추간판 탈출증의 한의학적 치료와 슬링운동 병행 치료 효과

양미성 ( Mi Sung Yang ),강대희 ( Dae Hee Kang ),기영범 ( Young Bum Ki ),박수곤 ( Su Gon Park ),조희근 ( Hee Geun Cho ),최진봉 ( Jin Bong Choi ),설재욱 ( Jae Uk Sul ),김선종 ( Sun Jong Kim ) 경락경혈학회 2010 Korean Journal of Acupuncture Vol.27 No.4

Objectives: Herniated lumbar intervertebral disc is common reason causing back pain. Lumbar stabilization exercise prevent back pain recurrence by strengthening lumbar muscles. Sling Exercise Therapy(SET) is a system for spinal muscle stabilization and strengthening using fixed string and mobile band. The purpose of this study is to investigate and measure the effectiveness of SET and oriental medicine for Herniated lumbar intervertebral disc. Methods: The subjects for this study are 40 patients suffering from Herniated lumbar intervertebral disc in our clinic. They were measured and compared by Visual Analogue Scale(VAS), 4 grades of recovery degree and sex, age, period of disease, disc herniation type, times of exercise. Results: In the above index, the score(VAS, 4 grades of recovery degree) improved after treatments. But they make no difference between sex, age, period of disease, disc herniation type and times of exercise. Conclusions: These results suggest that SET may be used for Herniated lumbar intervertebral disc for pain management and muscle strengthening as an essential treatment regardless of sex, age, period of disease and disc herniation type.