Ethanol중독 흰쥐에서 총담관결찰이 간의 Monoamine Oxidase 활성에 미치는 영향

정성광,곽춘식,Chung, Sung-Kwang,Kwak, Chun-Sik 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.3

이 연구는 간담도 질환시 음주의 해로움에 대한 생화학적 배경의 일단을 밝히고자 시행한 실험으로서 급성 및 만성 주정 중독을 시킨 흰쥐에게 당즙울체를 야기시켜 간의 monoamine oxidase(MAO) 활성도를 측정한 것이다. 흰쥐에게 만성 주정 중독을 시켰을 때 간의 mitochondrial 5-hydroxytryptamine-MAO(5HT-MAO) 및 benzylamine-MAO(Benz-MAO)의 활성도는 각각 약 41% 및 약 42% 증가하였으며, 급성 주정 중독을 시켰을 때는 mitochondrial BenzMAO의 활성도가 약 35% 증가하였다. 총담관결찰로 담즙울체만 야기했을 때는 간의 mitochondrial 5HT-MAO의 활성도가 총담관결찰 후 3일에는 약 37%, 7일에는 약 38%, 14일에는 약 57% 감소하였으며, 또한 mitochondrial Benz-MAO의 활성도도 이와 같은 경향으로 감소하였다. 만성 주정 중독 후 총담관결찰로 담즙울체를 야기했을 때 및 총담관결찰 후 급성 주정 중독을 시켰을 때 간의 mitochondrial 5HT -MAO 및 Benz-MAO는 주정 중독을 시켰을 때 보다는 그 활성도가 낮았으나 담즙울체만 시켰을 때 보다는 그 활성도가 높았다. 그리고 microsomal MAO들은 주정 중독을 시켰을 때만 그 활성도가 약간 증가되었다. 이상 성적으로 보마 간의 MAO는 주정 중독시에 그 활성도가 증가되는 효소이며 또한 담즙울체시 주정 중독이 야기되면 이 효소의 활성도가 당즙울체만 있을 때 보다 증가되는 효소로 생각된다. 따라서 이 성적은 담즙울체로 간손상이 있을 때는 음주를 해서는 안된다는 것을 반영하는 자료라 할 수 있다. The activities of the hepatic monoamine oxidase (MAO) were studies when cholestasis was induced and acute ethanol intoxication developed, or cholestasis following chronic ethanol intoxication for manifestation of the biochemical background of alcohol intoxication in hepatobiliary disease. There were marked increases in the mitochondrial 5-hydroxytryptamine-MAO (5HT-MAO) and benzyla-mine-MAO (Benz-MAO) activities of the liver of the rats treated with chronic ethanol intoxication, and showed significant increase in the mitochondrial Benz-MAO after treatment with acute ethanol intoxication. When common bile duct was ligated in the rats, mitochondrial 5HT-MAO and Benz-MAO activities in the liver significantly decreased. When common bile duct was ligated after chronic ethanol intoxication and acute ethanol intoxication was done after common bile duct ligation, the activities of mitochondrial 5HT-MAO and Benz-MAO in the liver decreased more significantly than in the group only with ethanol intoxication was performed. However, the activities showed a higher degree on the same time points than the groups only with the common bile duct ligation. And microsomal 5HT-MAO and Benz-MAO activities in the liver showed significant increase when treated both with the chronic or the acute ethanol intoxication. According to the above results, the hepatic MAO seems to be an enzyme in which its activity increases in acute and chronic ethanol intoxication. And MAO in the liver is the enzyme with increased activities in ethanol intoxidation with cholestasis more than in cholestasis. The cause of the increase is the development of biosynthesis. Accordingly, these results will be the data supporting that alcoholic drink is enzymologically harmful in hepatobillary disease.

Ethanol 중독 흰쥐에서 총담관결찰이 간의 Monoamine Oxidase 활성에 미치는 영향

정성광,곽춘식 ( Sung Kwang Chung,Chun Sik Kwak ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.3

The activities of the hepatic monoamine oxidase (MAO) were studies when cholestasis was induced and acute ethanol intoxication developed, or cholestasis following chronic ethanol intoxication for manifestation of the biochemical background of alcohol intoxication in hepatobiliary disease. There were marked increases in the mitochondrial 5-hydroxytryptamine-MAO (5HT-MAO) and benzyla-mine-MAO (Benz-MAO) activities of the liver of the rats treated with chronic ethanol intoxication, and showed significant increase in the mitochondrial Benz-MAO after treatment with acute ethanol intoxication. When common bile duct was ligated in the rats, mitochondrial 5HT-MAO and Benz-MAO activities in the liver significantly decreased. When common bile duct was ligated after chronic ethanol intoxication and acute ethanol intoxication was done after common bile duct ligation, the activities of mitochondrial 5HT-MAO and Benz-MAO in the liver decreased more significantly than in the group only with ethanol intoxication was performed. However, the activities showed a higher degree on the same time points than the groups only with the common bile duct ligation. And microsomal 5HT-MAO and Benz-MAO activities in the liver showed significant increase when treated both with the chronic or the acute ethanol intoxication. According to the above results, the hepatic MAO seems to be an enzyme in which its activity increases in acute and chronic ethanol intoxication. And MAO in the liver is the enzyme with increased activities in ethanol intoxidation with cholestasis more than in cholestasis. The cause of the increase is the development of biosynthesis. Accordingly, these results will be the data supporting that alcoholic drink is enzymologically harmful in hepatobillary disease.

Ethanol중독 흰쥐에서 총담관결찰이 혈청 및 간의 Alanine Aminotransferase 활성에 미치는 영향

김여희,정성광,곽춘식 啓明大學校 醫科大學 1989 계명의대학술지 Vol.8 No.1

This study was intended to observe the effect of common bile duct ligation on serum and liver alanine aminotransferase(ALT) activities in rats suffering from acute and chronic intoxication of ethanol. For chronic intoxication of ethanol, the rats were fed 5%(v/v) ethanol instead of water for 60days. Common bile duct of the same group of rats were ligated with ethanol constantly being fed. The rats were killed on the 1st, 2nd, 3rd, 7th and 14th days of the procedure to measure the cytosolic and mitochondrial ALT activities of the liver. The serum ALT activities were also measured. For acute intoxication of ethanol, 4g of ethanol were administered orally per kg of body weight as a single dose. The rats were killed at the 1.5th and 24th hours of the procedure for study. On the 14th day following common bile duct ligation, the rats were acutely intoxicated with ethanol to be killed at 1.5 the and 24th hours for measuring the activities of the above enzymes. In terms of rats liver cytosolic and mitochondrial ALT activities, no significant increase was shown in either chronically intoxicated ethanol group or acutely intoxicate ethanol group. The liver cytosolic and mitochondrial ALT activities of the common bile duct ligation groups showed a significant decrease at the 7th and the 14th days of the ligation. And the groups of acute intoxication with ethanol which was done after 14 days of the common bile duct ligation, the rats showed considerable decrease in the activities. The activities, however, both the groups that recieved the common bile duct ligation after being chronically intoxicated with ethanol which was done after 14 days of the common bile duct ligation showed far less decreases on the same time points than the group only with the common bile duct ligation. The serum ALT activities showed slight increase in acutely intoxicated ethanol group. On the other hand, both the common bile duct ligation groups and the one the received the same ligation after being chronically intoxicated with ethanol showed remarkable increase in the activities. But the activity, the groups that received the common bile duct ligation after chronical ethanol intoxication showed far more significant increases on the 7th and 14th days than the groups only with the common bile duct ligation, considerable activity increases were also shown when the groups were acutely intoxicated with ethanol after the ligation.

흰쥐 재생간의 알콜대사효소들의 활성치

문교철,곽춘식,정성광,김여희 啓明大學校 醫科大學 1988 계명의대학술지 Vol.7 No.2

A study was made on the change in the activities of the following during 10 post-operative days: alcohol dehydrogenase of regenerating rat livers after 70% (median and left lateral lobes) partial hepatectomy. After partial hepatectomy in the rats, activities of regenerating liver alcohol dehydrogenase significantly increased in the sixth day. The activities of microsomal ethanol-oxidizing system in the regenerating liver markedly increased from the third day after partial hepatectomy. However, no significant changes in hepatic catalase was noted throughout the experiments. The activities of cytosolic and microsomal aldehyde dehydrognease in the regenerating liver significantly increased from the sixthe day and the third to the sixth day respectively, but mitochondrial aldehyde dehydrogenase showed no change.

Ethanol중독 흰쥐에서 총담관결찰이 혈청 및 간의 Aspartate Aminotranferase활성에 미치는 영향

김여희,곽춘식,정성광 啓明大學校 醫科大學 1990 계명의대학술지 Vol.9 No.1

This study was intended to observe the effect of common bile duct ligation on serum and liver aspartate aminotransferase (AST) activities in rats suffering from acute and chronic intoxication of ethanol. For chronic intoxication of ethanol, the rat were fed 5%(v/v) ethanol instead of waster for 60days. Common bile duct of the same group of rats were ligated with ethanol constantly being fed. The rats were killed on the 1st, 2nd, 3rd, 7th and 14th days of the procedure to measure the cytosolic and mitochondrial AST activities of the liver. The serum AST activities were also measured. For actue intoxication of ethanol, 4g of ethanol were administered orally per kg of body weight as a single dose. The rats were killed at the 1.5th and 24th hours of the procedure for study. On the 14th day following common bile duct ligation, the rats were acutely intoxicated with ethanol to be killed at the 1.5 and 24th hours for measuring the activities of the ;above enzymes. In terms of rats liver cytosolic and mitochondrial AST activities, no significant increase was shown in either chronically intoxicated ethanol group or acutely intoxicated ethanol group. The liver cytosolic AST activity of the group of common bile duct ligation after chronic ethanol intoxication showed slight decrease at the 14th day after the ligation. The liver mitochondrial AST activity of the group of common bile duct ligation after chronic ethanol intoxication showed marked decrease on the 14th day of the ligation. And the groups of acute intoxication with ethanol which was done after 14 days ofthe common bile duct ligation, the rats showed considerable decrease in the activities. The activities, however, both the groups that recieved the common bile duct ligation after being chronically intoxicated with ethanol which was done after 14 days of the common bile duct ligation showed far less decreases on the same time points than the group only with the common bile duct ligation. The serum AST activity showed slight increase in acutely intoxicated ethanol group. On the other hand, both the common bile suct ligation groups and the one that received the same ligation afterbeing chronically intoxicated with ethanol showed remakable increase in the activities. But the activity of the groups that received the common bile duct ligation after chronical ehtanol intoxication showed far more significant increases on the 7th and 14th days than the groups only with the common bile duct ligation, considerable activity increases were also shown when the groups were acutely intoxicated with ethanol after the ligation.

주정 중독 흰쥐에서 총담관 결찰이 간의 Mitochondria 및 Microsome의 총 Monoamine oxidase와 혈청의 Benzylamine oxidase활성에 미치는 영향

김여희,안광욱,곽춘식,정성광 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.1

The activities of the hepatic mitochondrial and microsomal total monoamine oxidase(MAO), and serum benzylamine oxidase were studied for cholestasis induced by common bile duct ligation and acute ethanol intoxication developed, or cholestasis after chronic ethanol intoxication for manifestation of the biochemical background of alcohol intoxication in hepatobiliary disease. There were marked increases in the mitochondrial and microsomal total MAO activities of the liver in the rats treated with chronic ethanol intoxication, and showed a significant increase in the mitochondrial total MAO after treatment with acute ethanol intoxication. When common bile duct was ligated in the rats, mitochondrial total MAO activity in the liver significantly decreased. When common bile duct was ligated after chronic ethanol intoxication and acute ethanol intoxication was done after common bile duct ligation, the activity of the mitochondrial total MAO in the liver decreased more significantly than the group only with ethanol intoxication was performed. However, the activity showed a higher degree on the same time points than the groups only with the common bile duct ligation. The serum benzylamine oxidase ectivity in the group with only common bile duct ligation or the ethanol intoxication with cholestasis were measured between the 7th and 14th day, with no difference between two groups. According to the above results, the hepatic MAO seems to be an enzyme in which its activity increases in acute and chronic ethanol intoxicaion. And total MAO in the liver is the enzyme with increased activity in ethanol intoxication with cholestasis more than in cholestasis. The cause of the increase is the development of biosynthesis. Accordingly, these results will be the data supporting that alcoholic drink is enzymologically harmful in hepatobillary disases.