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곽명국(Myoung Kuk Kwak),김다슬(Da Seul Kim),오광석(Kwang-suk Oh),서영완(Youngwan Seo) 한국생물공학회 2014 KSBB Journal Vol.29 No.4
In this study, crude extract of the seagrass Zostera japonica, and its solvent-partitioned fractions were evaluated for their antioxidant activity. The crude extract was successively fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions by liquid-liquid partition. These include DPPH radical scavenging, hydroxyl radical scavenging in HT-1080 cells, peroxynitrite scavenging, and protective effect on DNA damage caused by hydroxyl radicals generated. In all assays, except for DPPH radical, 85% aq.MeOH and n-BuOH fraction showed the strong antioxidant activity. These results suggest that Z. japonica may be used as a potential source of natural antioxidants for the development of cosmetic product or functional food in the future.
키틴분해 박테리아 Bacillus idriensis (CGH18)의 항산화 활성
정명은(Myoung Eun Jung),홍주완(Joo Wan Hong),이정임,곽명국(Myoung Kuk Kwak),김호준(Hojun Kim),손재학(Jae Hak Sohn),송영선(Young-Sun Song),오광석(Kwang-suk Oh),서영완(Youngwan Seo) 한국생물공학회 2013 KSBB Journal Vol.28 No.4
bacterium CGH18 exhibiting antioxidizing and chitin-degrading activities in the colloidal chitin culture medium was isolated from salt-fermented crab. This strain was identified as Bacillus idriensis based on 16S rDNA sequence homology search. Its crude extract was partitioned between n-BuOH and H₂O. The organic layer was further partitioned between CH₂Cl₂ and H₂O. Antioxidant activities of crude extract and its solvent fractions were evaluated using five different bioassay methods, including the degree of occurrence of intracellular reactive oxygen species (ROS), peroxynitrite scavenging (ONOO), and oxidative damage of genomic DNA. All fractions exhibited significant antioxidant activity in bioassay systems used.