고에너지 엑스선을 조사한 부갑상선의 세포막모델에서 칼슘이온의 생리학적 대사에 미치는 영향

고인호,여진동 한국방사선학회 2022 한국방사선학회 논문지 Vol.16 No.2

The initial co-transport and counter-transport permeate transport characteristics of calcium ion at epithelial cell membrane model in parathyroid which irradiated by high energy x-ray(linac 6 MV) was investigated. The epithelial cell membrane model used in this experiment was a polysulfonated copolymerized membrane of poly(PS-DVB: polystyrene-divinylbenzene). The difference of sorbed water in membrane, fixed carrier concentration(SO32-), initial pH value, OH- concentration were occurred at difference of Ca2+concentration and quantity of parathyroid hormone, respectively. The initial co-transport and counter-transport permeate flux of Cl-, OH-, Ca2+on fixed carrier concentration(SO32-) and initial pH value of irradiated membrane was found to be decreased than non-irradiated membrane. The initial co-transport and counter-transport permeate flux of Ca2+ on fixed carrier concentration (SO32-), initial pH value, OH- concentration in irradiated membrane were found to be decreased about 2.68 ∼ 6.87 times, about 1.42 ∼ 1.63 times, about 2.07 ∼ 1.672 times than non-irradiated membrane, respectively. As a result, the quantity of parathyroid hormone was decreased at irradiated membrane than non-irradiated membrane. The decrease of parathyroid hormone was occurred at hypoparathyroidism and osteoporosis, parathyroiditis, and so on. As the parathyroid hormone in epithelial cell membrane model were abnormal, cell damages were appeared at cell. 이 실험에 사용한 부갑상선의 상피 세포막모델은 PS-DVB(polystyrene-diviny lbenzene)로서 술폰화 혼성 중합 막으로 제조한 이온투과성 고분자막이며 이 고분자막(세포막모델)을 이용하여 고에너지 엑스선(6MV X선)을 조사할 때 막의 수분흡수율 차이, 고정전달자(SO32-)의 농도 차이, 초기 pH 차이, OH-이온의 농도 차이가 세포의 내부∙외부 사이에서 칼슘이온(Ca2+)의 농도 차이로 나타나게 되고 그 영향으로 부갑상선호르몬의 분비량과 생성량의 차이로 발생하게 되는데 이런 영향을 구명하기 위해서 상피세포의 세포막을 통한 칼슘이온(Ca2+)의 초기 병류성과 항류성 투과전달 특성에 관하여 연구를 하게 되였다. 36.5℃로 고정된 상피세포의 고분자 막(세포막모델)을 방사선을 조사할 때 막의 수분흡수율 차이로 인해서 고정전달자(SO32-)의 농도변화가 비 조사된 세포막모델보다 약 2.17배 정도 감소하였고 고정전달자(SO32-)의 농도 차이, 초기 pH 차이, OH-이온의 농도 차이에 따라서 혈중 칼슘이온(Ca2+)의 초기 병류성과 항류성 전달속도 변화가 비 조사된 세포막모델보다 각각 약 2.68배 ∼ 6.87배, 약 1.42배 ∼ 1.63배, 약 2.07 ∼ 1.67배 정도 감소하였다. 결국, 방사선 조사로 인해서 부갑상선의 상피 세포막에서 혈중 칼슘이온(Ca2+)의 초기 병류성과 항류성 전달속도변화가 비정상적으로 되어 상피세포의 장애가 발생하고 부갑상선호르몬의 분비량과 생성량 감소도 동반하게 되었다고 추론되었다.

X선의 지수감약 모델을 이용한 금속 필터의 반가층 측정에 관한 연구

고인호 제주한라대학 1995 論文集 Vol.19 No.-

o The result of this study using narrow beam (X - ray) 4cm x 4cm, FDD (focus detector distance) =100cm, FFD(focus filter distance) =50cm, and FDD ( filter detector distance) = 50cm were as follows : 1. In this study, three filters were used to measure H.V.L. Under X-ray tube Voltage 100kvp, and the result was 2. 16mm Al H.V.L. , 0.0926mm Cu H.V.L. , and it was impossible to measure H.V.L. with pb filter. 2. Under constant exposure state, nonfilter appeared to be the unstability of X - ray unit's output. 3. Because of heterochromatic X - ray, H.V.L curve had a exponential attenuation type. 4. The more high atomic number's metal was, the less thickness of H.V.L was.

감마선이 조사된 세포막모델을 통한 음이온의 능동 전달 특성

고인호,여진동 한국방사선학회 2015 한국방사선학회 논문지 Vol.9 No.4

The active transport characteristics of anions of cell membrane model which irradiated by 60Co γ-ray was investigated. The cell membrane model used in this experiment was a sulfonated copolymerized membrane of poly(1-methyl-4-vinylpyridiniumiodide-co-divinylbenzene : MeVP-DVBI). First, the initial flux of OH- and Cl-, Na+ of membrane which was not irradiated was decreased with increase of thickness of membrane 80-200㎛, increased with increase of NaOH concentration 0-0.5mol/L and MeVP-DVBI concentration 20-80% was increased with initial flux of OH- and Cl-, decreased with initial flux of Na+. Second, the initial flux of membrane which was irradiated was less than that. And the driving force of pH of irradiated membrane was significantly increased more than membrane which was not irradiated. The initial flux of the OH- ion was decreased with increase of H+ ion concentration. As selective transport of OH- and Cl- of cell membrane model were abnormal, cell damages were appeared at cell. 감마선이 조사된 세포막 모델에서 음이온의 능동전달특성을 연구하였다. 이 실험에 사용된 세포막 모델은 세포막의 구형단백질, 당단백질 등의 기능을 지니고 있는 친수성의 극성인 poly(1-methyl-4-vinylpyridinium iodide-co-divinylbenzene: MeVP-DVBI)분말을 세포막의 인지질에 해당하는 소수성의 극성인 polysulfone과 결합시킨 불균질의 복합막을 사용하였다. 첫 번째 조사되지 않은 막에서 OH-, Cl-, Na+의 초기플럭스는 실험조건을 제막의 두께 80-200㎛로 할 때 정비례로 감소하였고, MeVP-DVBI의 농도를 20-80%로 할 때 OH-, Cl-는 지수 값으로 증가하였고 Na+는 지수 값으로 감소하여 음이온(OH-, Cl-)의 능동전달을 증대시키고 NaOH의 농도를 0-0.5mol/L로 할 때 0-2mole/cm2․ h로 지수 값으로 증가하였다. 두 번째 조사된 막에서 OH-, Cl-, Na+의 초기플럭스는 대체적으로 조사되지 않은 막과 비교해서 감소하여 음이온의 능동전달이 감소하였는데 이는 방사선조사로 H+이온 농도의 증가를 가져와 음이온의 초기플럭스가 감소하였기 때문으로 생각된다. 조사된 막의 pH의 추진력은 조사되지 않은 막보다 유의성 있께 증가하였고 세포막모델에서 OH-와 Cl-의 능동전달특성이 비정상적이기 때문에 세포장해가 세포에서 발현된다.

진단용 X선 격자가 사진학적 특성에 미치는 영향에 관한 연구

고인호 제주한라대학 1999 論文集 Vol.23 No.-

To this experiment, when it is the grid or non-grid, the change of H-D curve is as follows : First, To grid(8:1), The more grid size is to decrease, the more the sensitivity and γvalue are to increase. The change of density is to log 0.9=0.66∼1.09. Second, To grid (10:1), the sensitivity and γvalue are the most to grid size(14"x14"). And, the grid size(10"x12") is more value than 14"x17". The change of density is to log 0.9=0.47∼0.94. : Third, To the grid(6:1), the sensitivity and γvalue are the most to grid size(14"x14"). And, the grid size(14"x17") is more value than 10"x12". The change of density is to log 0.9=0.81∼1.11. If it used to grid, the γvalue is to 0.4∼0.8. Without used to grid, the γvalue is to 1.0355.

Star test pattern을 이용한 진단용 X선관의 초점크기측정에 관한 연구

고인호 제주한라대학 1998 論文集 Vol.22 No.-

N D Using F = ------ X ----- equation, 57.3 (M-1) star test pattern (RMI 07-509) was measured to equivalent focal size. Experimental data was appeared to under paper. 1. After to fix exposure time and x - ray tube voltage, If x - ray tube current was increased, equivalent focal size was increased. 60kvp, IOOmA is to 1.039 X 1.059mm, 60kvp, 50mA is to 0.727 X 0.873mm, 2. After to fix exposure time and x-ray tube current, If x-ray tube current was increased, equivalent focal size was decreased. 50kvp is to 0.823 X 0.873mm, 70kvp is to 0.686 X 0.665mm,

감마선을 조사한 생쥐 간에서 발효인삼이 Glutathione 함량에 미치는 영향

고인호,Ko, In-Ho 대한방사선치료학회 2006 대한방사선치료학회지 Vol.18 No.1

Purpose: The radioprotective effects of white and fermented ginseng on liver damage induced by $^{60}Co\;{\gamma}$-ray were investigated. Materials and Methods: To one group of ICR male mice were given white(150 mg/kg/day for 7 days, orally) and fermented ginseng(150 mg/kg/day for 7 days, orally) before $^{60}Co\;{\gamma}$-ray irradiation. To another group were irradiated by 5 Gy(1.01 Gy/min) dose of $^{60}Co\;{\gamma}$-ray. Contrast group were given with saline(0.1 mL). The levels of reduced(GSH) and oxidized(GSSG) glutathione in liver tissue were measured. Results: In the fermented(150 mg/kg) and white ginseng(150 mg/kg) groups than irradiation group, the GSH levels were significantly increased, but the GSSG levels were significantly decreased. The ratio of GSSG/total GSH was significantly decreased in the fermented(150 mg/kg) and white ginseng(150 mg/kg) groups than irradiation group. Conclusion: In the fermented(150 mg/kg) groups than white ginseng(150 mg/kg) groups the GSH levels were significantly increased. The radioprotective effects of fermented(150 mg/kg) groups than white ginseng(150 mg/kg) groups were increased.

고 에너지 전자선의 근사함수를 통한 비교 연구

고인호 제주한라대학 2000 論文集 Vol.24 No.-

The high energy electron beam is used to therapy of skin cancer,Breast cancer, Tung cancer.etc. This study proposed a semianalytical method instead of Monte carlo method for dose calculations to Linac 6M velectron beam. An integral equation for the proximity function that can be solved by using information of the spatial dose distributions is gained. The proximity function for dose calculations of electrons(from 10eV to 1OKeV) in water is calculated by solving the equation numerically. The results are agreement with those obtained using the Monte Carlo method. The proposed method can be used for electrons of high energies more efficiently than the Monte Carlo method. To the same set of cross section, the proximity function t(x) (eV/㎍/㎠) obtained using the semianalytical method for 100ev, lkeV,10keV electrons for the values of 0.01∼10㎍/㎠ are agreement with those obtained using the Monte Carlo method.

조산아 중환자실에서의 흉부 X선 검사시 방사선 피폭에 관한 연구

고인호 제주한라대학 2001 論文集 Vol.25 No.-

There are few reports on the radiation dose received by infants, their family and radiological technologists exposed to scatter radiation in a premature intensive care unit(ICU). Therefore, A study is objective to evaluate the degree of radiation exposure from diagnostic x-ray examination with mobile x-ray machines in a premature intensive care unit(ICU). The radiation exposure of an adjacent newborn, the radiological technologist and oher persons in the room was simulated using phantoms during x-ray examination of the chest using vertical and horizontal beams by Hitachi 500mA, 250kvp, inverter type radiography unit instead of mobile x-ray machines. As a result, the following recommendations for the performance of x-ray examination in a premature intensive care unit(ICU) are made : 1. No additional protection for neighbouring premature infants is necessary. 2. The radiological technologist should wear a lead apron. 3. Parents and personnel need not interrupt their activities or leave the room during an x-ray examination. 4. When using a horizontal beam, the beam must be directed away from oher persons.

항공산업의 서비스품질 향상 전략연구 : 서비스지향성을 중심으로

고인호 慶熙大學校 大學院 院友會 2005 高凰論集 Vol.36 No.-

항공운송사업은 국가의 인적 물적 자원의 효율적인 분배를 가능하게 하여 다른 산업 분야의 경제찰동의 촉진에도 크게 기여할 수 있는 수송체계로서 고객확보를 위한 항공사간의 경쟁이 심화되고 있다. 본 연구에서는 우리나라 항공사의 서비스 지향성이 서비스의 제공과 품질에 미치는 영향을 분석 조사하였다. 그 결과 서비스 표준의 설계와 표준의 제공에 미치는 서비스 지향성의 영향력이 비교적 큰 것으로 나타났다. 또한 고객지향적 서비스 표준의 설계는 표준의 제공 및 항공사의 보조서비스 품질에 유의한 영향을 미치고 있는 것으로 나타났으며, 서비스 표준의 제공은 핵심/보조 서비스에 영향을 미치고 있는 것으로 나타났다. 마지막으로 보조서비스품질은 고객만족에 직접적인 영향을 미치는 것으로 나타났다. 본 연구의 결론은 실무적으로 서비스 가치의 전달은 서비스지향정-가치제공-서비스 품질-만족으로 연결되기 패문에 우리나라 항공사들이 지속적으로 경쟁력을 향상시키기 위해서는 서비스 지향성이 서비스품질향상과 고객만족을 위한 전략적 무기임을 인식하고 이를 향상시키데 노력해야 한다는 점을 시사하고 있다.

x선 필름 감광 유제성분인 gelatin의 산화 및 단백질 분석에 관한 연구

고인호 제주한라대학 1993 論文集 Vol.17 No.-

Microcomponents which contain cysteine, cystine and cysteic acid are supposed to be in gelatin. The most active compound seems to be cysteine. Gelatins were investigated by means of HPLC and fluorescence detection. The resulting data provide the sum of cysteine and cystine and by a second determination the sum of cysteine/cystine and cysteic acid content. In spite of the fact that gelatins originate from different manufacturers, it turned out that limed bone gelatins always contain less cysteine/cystine than limed hide gelatins. And, During oxidation of gelatin procedure, inorganic compounds, namely thiosulphate, sulphite and nitrite, are destroyed as well as the organic impurity cysteine. Under strong conditions, carbohydrates and aldehydes are oxidized too. Not only impurities will be oxidized, some amino acid of the gelatin molecule undergo oxidation as well. Methionine is easily oxidized, forming methionine sulphoxides, other amino acids are converted to aldehydes or to other compounds, respectively. And, gain growth increases with oxidaton. Addition of cysteine decreases the grain growth in oxidized gelatins. And, the amino acids tyrosine and phenylalanine have been determined id photographic gelatin using the technique of second drivative spectroscopy. Results confirm earlier reports that gelatin produced by lime process has a lower tyrosine content than gelatin produced by an acid process.