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Emerging Roles of RNA-Binding Proteins in Plant Growth, Development, and Stress Responses
강훈승,이관욱 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.3
Posttranscriptional regulation of RNA metabolism, including RNA processing, intron splicing, editing, RNA export, and decay, is increasingly regarded as an essential step for fine-tuning the regulation of gene expression in eukaryotes. RNA-binding proteins (RBPs) are central regulatory factors controlling posttranscriptional RNA metabolism during plant growth, development, and stress responses. Although functional roles of diverse RBPs in living organisms have been determined during the last decades, our understanding of the functional roles of RBPs in plants is lagging far behind our understanding of those in other organisms, including animals, bacteria, and viruses. However, recent functional analysis of multiple RBP family members involved in plant RNA metabolism and elucidation of the mechanistic roles of RBPs shed light on the cellular roles of diverse RBPs in growth, development, and stress responses of plants. In this review, we will discuss recent studies demonstrating the emerging roles of multiple RBP family members that play essential roles in RNA metabolism during plant growth, development, and stress responses.
Regulation of RNA metabolism in plant development and stress responses
정현주,박수정,강훈승 한국식물학회 2013 Journal of Plant Biology Vol.56 No.3
Posttranscriptional regulation of RNA metabolism, including RNA processing, splicing, editing, transport, translational control and turnover, is a key regulatory process in plant growth, development, and stress responses. A variety of RNA-binding proteins (RBPs) plays central roles during these cellular processes. Over the last decades, a considerable progress has been made in the identification and functional analysis of RBPs involved in growth, development, and stress response of plants. Identification of different family members of RBPs and determination of their functional roles in RNA metabolism shed light on the importance of the regulation of RNA metabolism and the role of RBPs as a central regulator in diverse cellular processes. In particular, recent reports demonstrate the emerging idea that certain RBPs perform a function as RNA chaperones during growth, development, and stress response of plants.
Sy Nguyen Dinh,박수정,한지훈,강훈승 한국식물학회 2019 Journal of Plant Biology Vol.62 No.1
Although accumulating evidence points to theessential roles of nucleus-encoded chloroplast S1 domaincontainingproteins (SDPs) in chloroplast RNA metabolismand plant development, functions of chloroplast SDPs inabiotic stress responses are largely unknown. In this study,we investigated the role of a SDP (At1g12800) in Arabidopsisresponse to diverse abiotic stresses. Analysis of the sdpknockout mutant and complementation lines demonstratedthat loss of SDP function results in decreased survival rate ofArabidopsis under salt, heat, UV, or freezing stress, but notunder dehydration stress or ABA. In consistent with aprevious report demonstrating that SDP is involved inchloroplast rRNA processing, translation in chloroplasts wasimpaired in the sdp mutant. Expression of several nucleargenes involved in stress response and adaptation was alteredin the sdp mutant subjected to different abiotic stresses,suggesting that modulation of chloroplast translation affectsthe expression of nuclear genes under abiotic stresses. Thesedata reveal that chloroplast-localized SDP plays an importantrole in abiotic stress response by modulating chloroplasttranslation and the expression of nuclear genes possibly viaunidentified plastid-to-nucleus signaling.
김지성,Tieu-Ngoc Nguyen Le,강훈승 한국식물학회 2017 Journal of Plant Biology Vol.60 No.3
Despite the fact that many nucleus-encoded RNA-binding proteins (RBPs) are targeted to chloroplasts and play essential roles in RNA metabolism in chloroplasts, the question of whether artificial targeting of a nucleus-encoded RBP to chloroplasts affects chloroplast function and plant growth has never been addressed. In this study, a nuclear zinc finger-containing Arabidopsis RBP, designated AtRZ1a, which was previously shown to play a role in stress response, was artificially targeted to chloroplasts, and the growth and stress response of the transgenic plants were evaluated. Confocal analysis of the cellular localization of the cTP_AtRZ1a protein containing the N-terminal chloroplast transit peptide (cTP) from rubisco small subunit revealed that the cTP_AtRZ1a fusion protein is successfully targeted to chloroplasts. When grown under normal conditions, flowering of the transgenic plants was delayed, and the FLC expression was significantly upregulated in the transgenic plants. Artificial targeting of AtRZ1a to chloroplasts severely inhibited seedling growth of the plants in the presence of ABA by upregulating expression of ABA signaling-related genes ABI3 and ABI4. Taken together, these results suggest that artificial targeting of a nucleus-encoded AtRZ1a to chloroplasts affects the growth and development of Arabidopsis under normal or ABA treated conditions.