http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
해조류 방사무늬김 (Porphyra yezoensis) 엽체로부터 산 유도 유전자의 분리
강세은 ( Se Eun Kang ),최재석 ( Jae Suk Choi ),박선미 ( Sun Mee Park ),박중연 ( Jung Youn Park ),진덕희 ( Duck Hee Jin ),홍용기 ( Yong Ki Hong ),( Long Guo Jin ) 한국수산과학회 2004 한국수산과학회지 Vol.37 No.4
N/A Genetic responses of the edible seaweed Porphyra yezoensis tissue to acid shock have been compared using differential display technique. The tissue was challenged in seawater containing 0.05% hydrogen chloride (pH 3.0) for 5 min, then rehabilitated in normal seawater for 10 min, 30 min, 60 min and 4 hrs. Total RNA extracted by the LiCI-guanidium method was reverse transcribed and amplified by PCR with arbitrary primers. The amplified fragment responded by the acid shock was selectively isolated from agarose gel and sequenced with DNA auto sequencer. Sequence (1056 bp) of the cDNA contained at least two genes for ASP7K (MW 7418) and ASP5K (MW 5512) proteins.