Glomus Tumor의 一例

黃祐典,楊春更,金春俊 최신의학사 1960 最新醫學 Vol.3 No.4

甲狀腺이 膵臟의 Langerhan氏嶋와 肝臟 및 筋肉의 糖代謝에 미치는 影響에 관한 實驗的 硏究

黃祐典 최신의학사 1960 最新醫學 Vol.3 No.2

Co^(60) 照射가 Ehrlich 腹水癌의 發育에 미치는 影響에 關한 實驗的 硏究

黃祐典 首都醫科大學 1965 수도 의대 잡지 Vol.2 No.1

Co^(60) 照射가 Ehrlich 腹水癌의 發育에 미치는 影響에 關한 實驗的 硏究

黃祐典 고려대학교 의과대학 1965 고려대 의대 잡지 Vol.2 No.1

Recent advances in radiological medicine have made the radiotherapy play an important role in cancer- control. As Fletcher has suggested Co^(60), the many gamma ray-emitting isotopes, has been found to be the most useful in the practice of radiotherapy. The beam of 1 kilocurie Co^(60) unit: is equivalent to that of a two million volts X-ray generator, and this isotope combines the advantage of skin-sparing effect, greater depth dose, less side scatter of radiant energy in better systemic tolerance, and decrease in differential bone absorption. Although extensive works have been done on the effects of Co^(60) irradiation on the dosage survival relation, aging phenomena, and radiosensitivity of mice, we know very little about histopathological research on the growth and organ infiltration of Ehrlich ascites tumor irradiated with Co^(60) isotope. The present study has been undertaken to observe the growth characteristics and :infiltrative properties of Ehrlich ascites tumor inoculated intraperitoneally after the irradiation of Co^(60) in vitro. Animals employed in this experiment were about 300 female adult mice of C.F.W. strain, weighing around 24 gms, and devided into two groaps, the control group and the irradiated group. Apparatus used for Co^(60) irradiation was C-3,000 TICKER Co^(60) TELETHERAPY UNIT, maintained by the Radiology Research Institute. The tumor cells per cubic mm. were counted and diluted to contain 5 million cells per ml in 0.85 per cells saline which was previously mixed with 1,000 units of dehydrostreptomycin and penicillin. One half of above cell suspension was exposed to Co^(60) radiation in vitro, and then one million cells per animal were inoculated intraperitoneally. The remaining half of the cell suspension was orovided as control, and the same number Gf cells per animal was inoculated. Body weight was measured every day throughout the tumor experiment. Four t o eleven mice from each group were sacrificed at 3, 6, 12, and 24 hours after the tumor cell inoculation and every 24 hours thereafter on throughout the period of 15 days. Ascitic fluid and abdominal organs were microscopically observed. Total volume of ascites was measured and the total number of tumor cells was counted. Both ,smears of ascitic fluid and tissue sections of intra-abdominal organs of each animal were prepared, and Papanicolaou and H.& E. stain were applied respectively for cytoIogical and histopathoIogical observation. The results of the irradiated group are summarized as follows: 1. Turbidity and viscosity of tumor ascitic fluid appeared to be decreased. 2. The total tumor cells in the ascites showed a considerable decrease in number, while degenerative tumor cells and inflammatory exudative cells were increased. 3. The number of normal mitotic figures of the tumor cells was decreased and, in contrast, that of abnormal mitotic figures was increased. 4. Histopathologically, infiltrative properties of the tumor cells in the irradiated group appeared to be weak and delayed in comparison with those in the control group. 5 . The tumor cell infiltration was most prominent in the abdominal wall, pancreas, mesentery, and pericapsular adipose tissue, and slight in the liver and spleen.

織維腺腫의 二例

具國會,黃祐典,薛奎東,徐廷翼 醫藥係社 1958 綜合 醫學 Vol.3 No.3

發癌性物質이 染色體에 미치는 影響에 關한 形態學的硏究 <第一報>

具國會,白大鉉,黃祐典 최신의학사 1958 最新醫學 Vol.1 No.1

Since 1941, the success of experimental formation of cancer with tar in rabbit's ears by Rous, a cane tissue would be produced optionally in animal. Other hand, Omuro obtained -cancer in plants by tar application and open an epoch of the cancer development i plants. Strong reported that the mutation of susceptibility on gastric cancer formed in reproductive cells an hand on to the posterity. Murrey and Little claimed ;'that afactor in breast cancer would be hereditary, and Bittner discribed carcinogenic factors are transmitted. from mother through the maternal milk. Berenblum and ibis co-workers reported that the MUSTARD SULFATE, noncarcinogenic, conformed as a strong causative substance for mutation supresser the cancer development when it was used concurrently with the carcinogenic substances. Tatum, and Demerec had a study on mutation by applying 20methylcholanthrene to Neurospora, -and Drospohila but no relationship between the hereditary behavior of mutation and the carcinogenic power was obsesved. Boyland proposes that, the carcinogenic substance act to nucleic acid on carcinogenic mechanism and carcinogenic action is connected with the specific changes in chromosomes. Notwithstanding the cause on the genesis of tumours, the somatic cells which constitute the individual vary in the tumour cells, specially in the malignant tumours, the normal cells vary in automatically and unrestrictedly growing neoplasm. Therefore, many observers emphasize that the cancer is produced by the mutation in somatic cells. Here, as it would be the very interesting: problem to study experimentally about the carcinogenic substances which occur the mutation of somatic and reproductive cells, that we decided to study on the above problem. For convinience, we observe in three divided parts; first part, the observation of chromosomes in the selected plants which is able to adminstrate the carcinogenic substance and present the wide physicechological variation. Second part, observation of the reaction on the administration of carcinogenic substance. Third part, -observation of its heredity. Firstly, -in this part, we studied precisely about the morphology of chromosomes of reproductive cells of Tradescantia. 1) MATERIALS AND METHOD Materials; A tradescantia family from the agrecultural college of Seoul National University and the Choong Rang Kyo farm of Dong Kook University. The rea ons why we selected this plant, are it shows wide physioechological variation and we surmice the plant may be represent many variation when the administration of carcinogenic substance, and the ' convinience to observation since its long efflorescence. Method: Pollem mother cells of the experimental plant which preserved in unified temperature and moisture are observed at its highest mitotic period, 4 -to $ a.m. by smear method and studied the movements of chromosomes microscopically after acetocarmine or hematoxylin stain. II) RESULTS AND . DISCUSSION The chromosome number of the plant are 2n=12, ,but there are sometiines,triploid or tetraploid. Generally, the higher value of the poliploid 'appear the higher activity of growth and the incidence of the higher vital power. The length of nuclei of the pollem mother cells in the, Diploid were 33 to 35 micron, and 40 to 70 M81 t 7} tl-Iq-.10 l Tmicron in that of the tetraploid. In mitotic figures of diploid, the basic number in this plant, 5 pairs of chromosome arranged in perfect order at its equator and a residual pair of chromosome which separated from the, 5 pairs act automatically,, and then, the chromosomes move to the both opposite poles before or after the 5 pairs of chromosome. In triploid, chromosomes reveals various complexity on its movements to the poles and in tetraploid, two pairs of uniquely acting chromnsomes are separated from the ordered ten pairs of chromosome. Above discription indicates that the chromosome number of tradescantia family are 2n=12 and the author propose the basic number of chromosome in this plant are five and the extra chromosome is infered from the other system on the developmental procedure of the plant. The "bridge", founded in triploid, is related to the above discribed fact and is imaginable as a failed result of crossing-over of the gene and prove the possibility of wide variation. SUMMARY The results of hereditary observation in normal tradescantia are as follows. a) The chromosome number of tradescantia are 2n=12, and X=5 is the-basic number of chromosome and possible the wide variation. b) The higher value of poliploid show the higher activity of growth and stronger vital, power.