大麥種子의 Amylase 생성에 미치는 Gibberellic Acid의 촉진효과와 Indole-3-acetic Acid의 억제효과의 解析

蔡麟基,鄭英淑 한국식물학회 1977 Journal of Plant Biology Vol.20 No.2

Using barley seeds (Hordeum sativum Jess, var.), the influences of gibberellic acid (GA) and idole-3-acetic acid (IAA) on the amylase synthesis and that of the nucleic acid metabolism were investigated. 1. With the deembryonized barley seeds, the increase of amylase treated with a 10^-5M of GA and the decrease of amylase treated with 10^-5M IAA were matched by a proportionate increase and decrease in the amount of RNA. The influence of the hormones on the RNA synthesis has appeared immediately after the treatment but on the amylase synthesis it has appeared 8 hours later. But no influence on the DNA synthesis was observed on both hormones. 2. The amylase from deembryonized barley seeds treated with GA and IAA have been fractionated by gel fiteration on Sephadex G-100. The amylase components showed four fractions on both enzymes treated with GA and IAA. Fraction Ⅰ(FI) was differed from fraction Ⅳ (FIV) in Km value and the effects of temperature, pH and metal ions. On the basis of their emzymatic properties, it was considered that the FI was β-amylase and FIV was α-amylase. The influences of GA and IAA on each fractions appeared to be similar but on the amylase units per souble protein, IAA inhibited the production of amylaes FIV while it promoted that of amylase FI. 3. An experment was conducted to determine whether IAA inhibits GA-promoted amylase synthesis competitively or non-competitively. Using a Lineweaver-Burk plot, it was clear that IAA was acting in a non-competitive fachion. From this, IAA was probably not competing with GA at the same site, but it was acting at some other site which resulted in partial blocking of the action of GA on the amylase synthesis.

大麥種子의 Amylase生成에 미치는 Gibberellic Acid와 Cinnamic Acid의 相互作用

蔡麟基,權貞愛 梨花女子大學校 韓國生活科學硏究院 1982 韓國生活科學硏究院 論叢 Vol.29 No.-

대맥 종자의, Amylase생성을 촉진하는 Gibberellic Acid(GA)에 대하여 생장 억제 물질로 알려진 Cinnamic Acid(CA)가 어떤 효과를 나타내는지를 알아보기 위하여 대맥 종자를 무균함 내에서 멸균된 면도날로 가로 반분하여 무배 반종자를 만들어 25℃, 암조건하에서 배양하면서 몇 가지 사항을 조사하여 보았는데 그 결과는 다음과 같았다. Amylase 활성은 Bertrand방법에 따라 생성 환원 당량으로 측정하였다. 1. GA(10^-5M)는 약 8시간의 지연기(lag period)를 거친 후 부터 amylase 생성을 활발하게 하여 배양 24시간에 최고치에 이르고 그후부터는 점차 감소하였다. 2. 대맥 종자의 amylase 생성을 유도하는 GA의 작용이 CA에 의하여 억제되었으며, 그것은 비교적 낮은 농도에서 이루어졌다. 3. CA의 억제 효과는 CA의 지연기 이후에 투여하여도 나타났었다. 4. GA 농도가 높아지면 CA의 억제 효과를 극복할 수 있었다. 5. 이미 생성된 amylase에 대하여서는 CA의 억제 효과가 없었다. This experiment was proceeded to examine the interaction of gibberellic acid(GA)and cinnamic acid on the amylase synthesis of barley seeds. The results were as follows: 1. After a lag period of about 8hrs, the activity of the amylase synthesis reached a maximum level in 24hrs od incubation and gradually decreased thereafter. 2. GA-induced amylase synthesis in barley seeds was inhibited by cinnamic acid, and this inhibition was effective even at a relatively low concentration. 3. The reduced enzyme activity caused by cinnamic acid in embryoless-half barley seeds was consistently maintained by the addition of GA even after the lag period. 4. The inhibitory effect of cinnamic acid was gradually removed by increasing the concentration of GA. 5. Cinnamic acid did not inhibit the activity of the presynthesized amylase.

표고(LENTINUS ODODES)의 生長에 미치는 Gibberellin의 影響

蔡麟基 이화여자대학교 한국문화연구원 1960 韓國文化硏究院 論叢 Vol.2 No.-

1. The present research was dealt with the elect of gibberellin on the growth of mycelium by the culture of Diploid and Haploid mycelia of Lentinus ododes in onion agar media mixed with 1ppm. 5ppm. 10ppm. and 50 ppm. of gibberellin. 2. Gibberellin inhibited the growth of mycelium in concentration mentioned above and it was found the higher concentration is the more inhibiting effect is. Furthermore, Diploid mycelia were inhibited by the gibberellin more than Haploid mycelia. 3. The effect of gibberellin on the growth of fruit body of Lentinus ododes was also studied with four kinds of concentration; 1ppm. 5ppm. 25ppm. and 50ppm. The result was that there were no significant differences among the concentrations mentioned above.

Chlorella의 增殖에 미치는 魚類의 影響

蔡麟基,韓勝熹 이화여자대학교 한국문화연구원 1963 韓國文化硏究院 論叢 Vol.4 No.-

There experiments which were taken place from August, 1936 to October, 1936 and were divided into 5-8 experimental groups with Chlorella vulgaris as the material established the following conclusions. These experiments were to examine the result of putting fishes in the fish pond for the purpose of finding out the method of good raising and of prompt breeding in practical culture of Chlorella. 1. Putting fish (crucian) in is effective for good raising and prompt breeding of Chlorella. In the temperature of September the amount of 10-20g of fish for every 1000cc of culture media acts maximum effect. It shows 3 or 4 times of breeding proportion as it is compared to control. 2. The effect for breeding of putting-fish is due to supplement of CO₂from their respiration. It is not resulted by crucian's secreation and excretion. 3. Accordingly when a farmer breeds Chlorella in his simple provision, the problem of supplement of CO₂can be solved easily and economically. And this method can serve both the fish-breeding and prevention from the development of mosquito larvae.

Chlorella의 增殖에 미치는 Gibberellin의 效果

蔡麟基,李蓮河 이화여자대학교 한국문화연구원 1966 韓國文化硏究院 論叢 Vol.8 No.-

Gibberellin has been known to be an agent which accelerates the growth of the higher plants. In order to see whether or not Gibberellin effected the multiplication of Chlorella(green algae), which is a lower plant, five experimental and one control series were set up. Each of five experimental series was made up using Myer's 4N-A5 Nutrient Solution which was 1ppm, 5ppm, 10ppm, 50ppm, and 100ppm of Gibberellin, respectively. Cultivation, with intermittent shaking for 10-21days, was carried out for each series. The results of this experiment showed the multiplication of Chlorella in the experimental series to be greater than that found in the control series. Multiplication was proportional to the concentration of Gibberellin. The plant multiplication in the experimental series in which 100ppm of Gibberellin was added, up to five to eight times that found in the control series.

大麥種子의 Amlase 생성에 미치는 Gibberellic Acid의 影響(第二報)

蔡麟基,李蕙淑 梨花女子大學校 韓國生活科學硏究院 1973 韓國生活科學硏究院 論叢 Vol.10 No.-

大麥種子 發芽時의 amylase생성이 胚物質 또는 GA에 의하여 誘導되는데 關한 이 實驗을 綜合하여 보건데 아래와 같이 要約할 수 있을 것 같다. 1) 胚物質보다 GA (5ppm)의 誘導效果가 더욱 높았다. 2) 胚乳에서의 amylase생성에는 呼吸의 增大가 반드시 수반되었다. 3) 酸化的燐酸化를 沮害하면 amylase생성이 抑制되었다. 4) GA의 amylase생성 誘導작용을 滅退시키는 DNP의 작용은 그 投與시간이 늦어지는데 따라 弱化되었다. 5) GA나 胚物質의 amylase생성 誘導作用은 加熱에 의하여 減少되는데 그 程度가 GA보다 胚物質에서 매우 甚하였다. 6) 따라서 amylase생성을 유도하는 大麥種子의 胚物質이 GA라고 斷定하기는 어려울 것 같다. The present investigation was designed to examine the effects of embryo substance or gibberellic acid(GA) on the amylase synthesis in the seeding of barley seed. The results are given below: 1) The inducing efect of GA on the amylase synthesis was found to be more conspicuous than embryo substance. 2) The amylase synthesis in barley endosperm was accompanied by increase of respiratory activity. 3) The amylase synthesis was suppressed when the oxidative phospholation was inhibited by DNP. 4) The DNP action suppressing the GA activity became weeker as the interval of GA and DNP dosage time were getting longer. 5) The inducing dffects of GA or embryo substance on amylasee synthesis was reduced when they were heated and this appeared much more considerable in the case of embryo substance than of GA. 6) The embryo substance inducing the amylase synthesis in barley seed might not be GA.

보리種子의 Amylase生成에 미치는 Gibberellic Acid와 Benzoic Acid의 相互作用

蔡麟基,黃末淑 梨花女子大學校 韓國生活科學硏究院 1980 韓國生活科學硏究院 論叢 Vol.26 No.-

식물 생장 호르몬의 일종인 gibberellic acid (GA)와 식물 생장을 억제하는 것으로 알려진 phenol계통의 benzoic acid (BA)가 식물 생장에 있어서 어떤 상호작용을 나타내는지를 알아보기 위하여 보리종자의 amylase생성에 미치는 영향을 대상으로 실험하였다. 그 결과 다음과 같은 사실들을 알 수 있었다. 1. GA(10^-5M)는 약 8시간의 지연기(lag period)를 거친 후 부터 amylase생성을 활발하게 하여, 배양 24시간에 최고치에 이르게 하였고, 그후부터는 점차 감소하게 하였다. 2. BA는 GA에 의한 amylase생성을 억제 하였다. 3. BA의 억제효과는 GA에 의한 지연기 후에 투여하여도 나타났었다. 4. amylase생성에 있어서의 GA의 촉진효과와 BA의 억제효과는 경쟁적이어서 어느 한쪽의 농도가 높아짐에 따라 다른 한 쪽의 효과를 극복하였다. 5. 이미 생성된 amylase활성은 BA에 의하여 영향을 받지 않았다. This experiment was proceeded to examine the interaction of gibberellic acid(GA) and benzoic acid on the amylase synthesis of barley seeds. The results were as follows: 1. After a lag period of about 8hr., the amylase synthesis reached a maximum level in 24hr. of incubation, and then leveled off. 2. GA-induced amylase synthesis in barley seeds was inhibited by benzoic acid. 3. The reduction of enzyme activity caused by benzoic acid in embryoless-half seeds of barley could be completely reversed by the addition of GA. 4. The formation of the enzyme could also be arrested by the addition of the benzoic acid in mid-course during amylase synthesis. 5. Benzoic acid did not inhibit the activity of the presynthesized amylase.

Chlorella의 生長과 ChlorophyII 含量에 미치는 微量元素 缺乏의 影響

蔡麟基,沈雄燮 이화여자대학교 한국문화연구원 1967 韓國文化硏究院 論叢 Vol.11 No.-

In order to see if the growth of chlorella cells and chlorophyll content are influenced by the deficiency of microelements(Fe, Mo, Mn, Cn, and B), Chlorella ellipsoidea was inoculated on the normal medium (as a control) and each microelement-deficient medium and has been cultured for six days at 20-25℃ with 10,000 lux. As the results of the experiments, chlorella cells on microelement-deficient medium showed that packed cell volume cell volume and chlorophyll content were reduced in 6(Cu)~35%(Fe), 10(Cu)~50%, and 13(Zn)~43%(Fe), respectively, in comparison with the control, and the order of the influence of the deficiency is Fe>Mo, Mn, B, Zn>Cu.

Chlorella의 生理에 미치는 Abscisic Acid(ABA)의 影饗

蔡麟基,鄭英淑,金年玉 梨花女子大學校 韓國生活科學硏究院 1979 韓國生活科學硏究院 論叢 Vol.22 No.-

Chlorella의 생리에 미치는 Abscisic acid(ABA)의 영향을 살펴보기 위하여 ABA의 몇가지 농도에 따른 Chlorella의 생장, 엽록소 함량, 광합성 및 호흡능, 그리고 단백질과 핵사 합성의 변화를 실험한 결과 다음과 같은 결과를 얻을 수 있었다. (1) Chlorella의 생장은 ABA 10^-3M농도에서는 억제되었으나 10^-5M농도에서는 별 차이가 없었다. (2) 엽록소 함량은 10^-3M농도에서 엽록소 a와 b가 모두 뚜렷이 감소되었으나 10^-5M농도에서는 모두 약간의 감소가 있었을 뿐이었다. (3) 광합성 및 호흡은 10^-3M에서는 모두 억제되었고 10^-5M농도에서는 별 변동이 없었다. (4) 단백질 함량은 10^-3M에서는 모두 감소하였는데 10^-5M농도에서는 별다른 증감이 없었다. (5) 핵산 함량 중 RNA는 10^-3, 10^-5농도에서 모두 감소되었는데 DNA는 어느 농도에서나 약간의 감소 경향을 나타내었을 뿐 뚜렷한 차이는 나타내지 않았다. (6) 결국 10^-3M농도의 ABA는 Chlorella의 생장을 억제하였는데 이것은 광합성 및 호흡의 억제, 그리고 나아가서는 체물질합성의 억제 특히 단백질과 핵산의 감소에서 오는 결과 때문인 것으로 보여진다. The experiment was proceeded to examine physiological effects of abscisci acid (ABA) on growth, chlorophyll contents, photosynthetic and respiratory activity, and changes in protein, necleic acid synthesis of chorella cells. Results were as follows : 1. The growth of Chlorella was decreased by 10^-3M ABA but was not significantly affected by 10^-5M. 2. Contents of chlorophyll a and b were apparently decreased by 10^-3M ABA and were slightly decreased by the 10^-5M. 3. Both photosynthetic and respiratory activities were inhibited by 10^-3M while the activities at 10^5M were not mush diffrent from thosse of the control. 4. 10^-3M ABA decreased protein content and 10^-5M did not significantly decrease. 5. RNA was descreased as ABA concentration increased and then DNA was slightly decreased as each concentration increased. It indicates that 10^-3M ABA restrained the growth of Chlorella by depressing photosynthetic and respiratory activity, biosynthesis of protein and nucleic acid.

Chlorella의 生理에 미치는 Cytokinin의 影響

蔡麟基,劉磨利 梨花女子大學校 韓國生活科學硏究院 1980 韓國生活科學硏究院 論叢 Vol.25 No.-

Chlorella의 생리에 미치는 Cytokinin의 영향을 살펴보기 위하여 Cytokinin으로 처리한 Chlorella의 생장, 엽록소 함량, 광합성과 호흡 등, 그리고 탄수화물, 핵산, 단백질 등의 함량 변화를 조사한 결과 다음과 같은 사실들을 알 수 있었다. 1) Chlorella는 Cytokinin 10^-5M에서 생장이 촉진되었다. 2) 엽록소 함량은 뚜렸한 변화가 없었다. 3) 광합성과 호흡이 촉진되었는데, 특히 호흡에서 현저하였다. 4) 탄수화물, RNA, DNA 그리고 단백질의 함량이 증가되었는데, 특히 RNA의 증가가 현정하였다. 따라서, Cytokinin처리에 의한 Chlorella의 생장 촉진은 광합성과 호흡의 촉진에 따른 탄수화물, RNA, DNA 및 단백질의 생합성 증진에 연유된 것으로 보여진다. By treating the cytokinin, the physiological influence on the growht, photosynthesis & respiratory activity, and on the amounts of chlorophyll, carbohydrate, nucleic acid and protein of Chlorella were invetigated. 1. Growth of Chlorella promoted at 10^-5 M of cytokinin. 2. There was no obvious change on the amount of chlorophyll. 3. Respiration was more increased in contrast to photosynthesis. 4. Amount of carbohydrate, RNA, DNA and protein were increased, especially RNA predominantly. Therefore, the result of experiment can be concluded in promotion of growth on the Chlorella due to increase in carbohydrate, RNA, DNA and protein synthesis in accordance with echancement of photosynthesis and respiration.