고구마의 경정조직 유래 기내 소식물체의 생장촉진과 순화

은종선,김영선,Shiro Higashi 한국식물생명공학회 1999 식물생명공학회지 Vol.26 No.2

고구마의 기내 소식물체의 생장촉진 및 순화율을 높이기 위하여 마디를 광혼합영양과 종속영양, 광독립영양 조건하에서 각각 배양하였다. Shoot길이는 3$0^{\circ}C$의 종속영양배양에서 11.9 cm로 가장 길었고 $25^{\circ}C$의 혼합영양배양에서 3.4cm로 가장 짧았다. 3$0^{\circ}C$에서 배양된 마디의 생육이 가장 빨라 마디수가 10.5개로 가장 많았지만 식물체는 도장되어 연약하였다 엽면적은 MF-처리구인 종속영양배양보다 MF+처리구인 혼합영양배양에서 2배로 넓었고 shoot의 건물률도 약간 높게 나타나 함수량이 비교적 적은 편이었다. 투명화된 불량묘는 광혼합영양배양의 경우 $25^{\circ}C$와 3$0^{\circ}C$ 모두 14.8%였고, 종속영양배양에서는 $25^{\circ}C$에서 22.2%, 3$0^{\circ}C$에서 31.5%로 MF+처리구에서 훨씬 적게 나타났다. Sucrose-free배지의 경우 MF-처리구에서 배양 30일 후에 마디절편이 노랗게 변하고 결국 고사되었으나 MF+처리구의 광독립영양배양에서는 정상적인 식물체로 생장되었다. 또한 sucrose와 호르몬이 첨가되지 않은 MS기본배지에서 MF+처리구의 경우 마디절편의 생존율은 양호하였다. The single node cuttings of sweet potato (cv. Mokpo #29) plantlets maintained in vitro were cultured with (MF+) or without membrane filter (MF-) under photomixotrophic (PM), hetrotrophic (HT) and autotrophic (AT) conditions. Shoot length was the greatest (11.9cm) in 3$0^{\circ}C$ (HT) treatment and it was the shortest (3.4 cm) in $25^{\circ}C$ (PM) treatment. Nodal explants cultured in 3$0^{\circ}C$ treatment looked more vigorous than those of $25^{\circ}C$ in appearance, and node number was the greatest (10.5 per plantlet) among the treatments. But plantlets grew in 3$0^{\circ}C$ (HT) treatment were observed all overgrown. The size in leaf area was about 2 times greater and shoot length was about 2 times shorter in PM than in HT condition. Percent dry matter of shoots was 5.9% (HT) and 7.4% (PM) in $25^{\circ}C$ treatment and 6.1% (HT) and 7.4% (PM) in 3$0^{\circ}C$ treatment. Plantlets cultured in the MF+ treatments were less succulent than those cultured in the MF- treatment. Vitrified plantlets were examinated 14.8% (both $25^{\circ}C$ and 3$0^{\circ}C$) in PM condition and 22.2% ($25^{\circ}C$) and 31.5% (3$0^{\circ}C$) in HT condition. Sucrose was necessary for the survival of in vitro plantlets. In the sucrose-free medium, explants cultured in the MF- had turned yellow and were dead after 30 days of culture. But explants cultured in the MF+ were alive and produced plantlets with shoot and root (AT). On the other hand, the survival of explants on the MS basal medium (sucrose-free and hormone-free) depended entirely upon the MF attachment.

生長點 및 Callus培養에 의한 딸기의 急速增殖에 관한 硏究

殷鐘旋 圓光大學校大學院 1982 學位論叢 Vol.8-2 No.-

In order to determine the induction of callus, the proper media for the differentiation of organs, the histological origin of multiple shoots and the virus infection, the meristem tissues and anthers of strawberry have been cultured on modified Murashige & Skoog's and Linsmaier & Skoog's media containing various levels of growth regulators. The results obtained were as follows: Calluses were induced from the cut ted side of the meristem tissue or filament and the surface of meristem tissue, and they could be classified into two types of friable nd knobby callus. The best callus growth was observed in the media containing 0.2-2㎎/ℓ NAA and 2,4- D plus 2㎎/ℓ kinetin and BA. Meristem tissue was developed into a plantlet at 0.2㎎/ℓ kinetin alone, but on the media with 2㎎/ℓ BA and kinetin added 0.2㎎/ℓ NAA, and both cases not added NAA caused vigorous formation of multiple shoots from a meristem, Somatic callus tissue formed by anther culture was also induced to form the multiple plantlets from the differentiation of adventitious bud. After 4 weeks in culture the adventitious roots were formed from callus tissue or bases of shoots on media supplemented with 0.2㎎/ℓ 2,4-D and NAA plus 2-4㎎/ℓ kinetin, and 8 weeks later most of the large shoots formed roots. The shoot primordia forming multiple shoots were differentiated from parenchyma tissue beneath the apical meristem, axillary bud, epidermis of leaf blade, vascular bundle of petiole and callus tissue. After 5 months of culture, multiple shoots were developed into 40-50 plantlets from an inoculum and after then proliferated rapidly. The hardened plantlets were transfered into the pot with the sterilized vermiculite, and were examined to determine whether the viruses were freed or not by leaflet-grafting to Fragaria virginiana and F. vesca So far, viruses were not detected in the grafted indicator plants.

상사화의 기내증식에 미치는 배양부위와 생장조절물질의 영향

은종선,김영선,박종숙,金松南,曹后男 한국식물생명공학회 2002 JOURNAL OF PLANT BIOTECHNOLOGY Vol.29 No.3

Lactuca bungeana의 資源開發에 관한 硏究 : I.雌雄性配偶體形成 및 受精現象 I.Formation of male and female gametophyte,and fertilization

殷鍾旋 전북대학교 농업과학기술연구소 1978 農大論文集 Vol.9 No.-

The present experiments were carried out to investigate the male and female gametophyte formation, and fertilization of Lacutuca bungeana which is a resource plant for vegetable crops. the results are summarized as follows; 1. Meiotic division of pollen mother cell is regular, and its tetrad is a tetrahedral type. 2. After early microspore stage the tapetal tissue is detached from the microsporangial wall and envelopes the microspores, and at the mature pollen stage it is disappered completely. 3. Ovule shows a anatropous type and integument is unitegumic. Nucellar type is a tenuinucellate type. 4. The arrangement of megaspore tetrad is of linear type. The micropylar end megaspore of linear tetrad is developed into embryo sac. 5. Embryo sac is a monosporic 8-nucleate polygonum type. 6. After pollination pollen grain is germinated immediately and the growth type of pollen tube is a monosiphonous. 7. Fertilization takes place usually within the blooming date, and in 7 days after pollination mature seed is developed.

고구마 정단분열조직 유래 식물체의 기내 증식에 미치는 배양조건의 영향

은종선,김영선,박종숙 한국식물생명공학회 2002 JOURNAL OF PLANT BIOTECHNOLOGY Vol.29 No.1

고추 약배양에서 배지조성 및 온도처리와 품종에 따른 배발생 (胚發生) 및 식물체 분화율과 유전적 변이

은종선,이광식,윤여중 한국육종학회 1994 한국육종학회지 Vol.26 No.4

도라지 배양묘의 생장 및 형태형성에 미치는 발광다이오우드의 효과

은종선,김영선,김용현 한국식물생명공학회 2000 식물생명공학회지 Vol.27 No.1

발광다이오우드 (Light emitting diode)를 사용한 적색, 녹색 및 청색광에서 식물생산의 가능성을 조사하기 위하여 도라지 유묘의 생장과 형태형성에 미치는 광질의 효과와 광질에 따른 엽록소함량을 형광등과 비교하여 조사하였다. 식물체의 초장은 형광등에서 3.8 cm로 가장 짧았고 LEDs 에서 생장한 것은 적색광에서 13.4cm로 가장 길었다. 반면에 적색/청색의 혼합광은 5.6cm로 적색 단색광에서 식물체가 도장된 것에 비해 정상적인 식물체의 생장양상을 보여 적색과 청색의 혼합광이 식물체의 생장에 적당하였다. 엽면적은 녹색광에서 24.1 $\textrm{cm}^2$로서 적색광에서 10.1 $\textrm{cm}^2$인 것에 비하여 약 2.4 배가 더 넓어 다른 처리구에 비하여 가장 양호하였다. 건물률은 적색/청색의 혼합광에서 15.3%를 나타내어 다른 광질처리보다 함수율이 적었다. 엽록소함량은 청색 단색광과 적색/청색의 혼합광에서 형광등보다 각각 20%, 10% 적었으나 적색과 녹색의 경우 각각 2%, 7% 적었는데 적색 단색광에서 형광등과 비슷한 엽록소 함량을 보였다. To clarify the possibility of plant production under red, green. blue, and red+blue using light emitting diodes (LEDs) and fluorescent lamps (control), the effects of light quality on the growth and morphogenesis of in vitro seedlings in Piatycodon grandiflorum were examined. The plantlets grown under the LEDs resulted in taller plants with greater stem than fluorescent lamps. The shortest shoot length, 3.8 cm, was observed in the control and the longest one, 13.4 cm, in the red light. But the shoot length was 5.6 cm under red LED with supplemental blue(red+blue light). This results indicate that red LED may be suitable, in proper combination with other wavelengths of light. The root length under red light was significantly smaller among the treatments. The plantlets grown under red+blue light had lower shoot dry weight, higher dry matter than other lights-grown plantlets. Among the various growth parameters measered, there was an indication that leaf area was controlled by the LEDs. Leaf area of a plantlets developing under green light was about 2.4 times wider than that of plantlets grown under red LED (10.1 $\textrm{cm}^2$ in area). The dry matter rate per plantlet among the treatments was greater in plantlets grown under the red/blue LEDs in comparison with that grown under other LEDs. Chlorophyll contents in plantlets grown under the red, green, blue and red/blue LEDs were 2%, 7% 20% and 10% lower, respectively, than those in plant grown under fluorescent lamps.

준고냉지의 채소재배에 의한 소득증대

은종선,Eun, Jong-Seon 한국과학기술단체총연합회 1981 과학과 기술 Vol.14 No.11

고추의 組織培養에 미치는 植物生長調節濟의 影響

殷鍾旋,金明準 全北大學校 農村社會發展硏究所 1991 農村社會硏究 Vol.2 No.-

These experiments were performed to investigate the effects of plant growth regulators on the tissues culture of red pepper. The best callus growth was observed in Murashige & Skoog's media containing 0.2-2.0㎎/l 2, 4-D plus 0.2㎎/l kinetin or BA on the segments of hypocotyl and cotyledon of red pepper. On the medium with 2.0㎎/l zseatin and 0.01㎎/l 2, 4-D somatic embryos were differentiated from embryogenic cell of immature embryo by suspension culture after 5 week-culture and 8 weeks later they were developed into plantlets, while on the medium with 1.0㎎/l BA and 0.05㎎/l NAA the shoot and root were differentiated from callus mass. The addition of brassinolide retarded the formation of somatic embryo, shoot and root. Key words : Brassinolide - BA - Capsicum annuum(red pepper) - embryogenic callus - 2, 4 - D - kinetin - NAA - somatic embryo - zeatin.

組織培養에 의한 딸기 無病株 育成에 관한 基礎硏究 : II.生長點培養에 의한 器官分化 및 無病株의 急速增殖 II.Organ Differentiation and Rapid Clonal Multiplication of Virus-free Stocks in Meristem Culture

殷鍾旋,李炳基,韓光洙,高正愛 전북대학교 농업과학기술연구소 1982 農大論文集 Vol.13 No.-

In order to determine the production of virus-free stocks from virus infected strawberries, meristem tissue has been cultured in tile media with various levels of growth regulators. According to this experiment, the callus induction, the organ differentiation, the origin of multiple shoots and the determination of virus-free stock were examined. The results obtained are as follows: Calluses were formed at the cuffed side and surface of inoculum and vigorous callus induction were occured on media supplemented with 0.2㎎/ℓ NAA or 2,4-D plus 2㎎/ℓ kinetin or 6-benzyl-adenine(BA). The formation of multiple shoots was found to be the best media containing 0.2㎎/ℓ NAA plus 2㎎/ℓ BA or 2㎎/ℓ kinetin alone. As origin of multiple shoots was observed histologically, the shoot primordia forming multiple shoots were differentiated from original apical meristem of inoculum, parenchyma tissue beneath the apical meristem, axillary buds, epidermis of leaf blade, vascular bundle of petiole and callus tissues. After 5 months of culture, multiple shoots were developed into 40∼50 plantlets from an inoculum and after then proliferated rapidly. The hardened plantlets transfered into the pot with sterilized vermiculite, and were examined to determine by the leaflet grafting to Fragaria virginiana and F. vesca (UC-1) whether the viruses were freed or not. So far. viruses were not detected in the grafted indicator plants.