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Expression of Glycolate Oxidase Gene in Spinach
박양서,최정도,조남정,Park, Yang-Seo,Choi, Jung-Do,Cho, Nam-Jeong 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.3
시금치 glycolate oxidase 유전자의 성질을 Southern 그리고 Northern blot hybridization 방법으로 조사하였다. EcoRI 또는 HindIII 제한효소로 처리한 genomic DNA를 시금치 glycolate oxidase cDNA를 probe로 사용하여 Southern blot 방법으로 분석한 결과 여러 개의 band가 관찰되었다. 이 효소 유전자의 발현양상은 Northern blot 방법으로 분석하였다. 이 유전자의 발현활성은 파종 후 8-35일 동안 비슷하였다. Glycolate oxidase mRNA는 잎에서는 많이, 그리고 줄기에서는 약간 발견되었으나, 뿌리에서는 거의 발견되지 않았는데, 이 결과는 이 효소 유전자의 발현이 조직에 따라 다름을 나타낸다. Glycolate oxidase mRNA의 양은 식물을 암 소에서 배양하였을 때는 상당히 감소하였으나, 빛을 쪼여주자 그 양이 증가하였다. 이련 결과는 빛이 glycolate oxidase 유전자의 발현올 촉진한다는 것을 보여준다. The nature of glycolate oxidase gene in spinach was investigated by Southern and Northern blot hybridization. Genomic DNA digested with EcoRI or HindIII exhibited multiple bands when hybridized with a spinach glycolate oxidase cDNA. Expression patterns of the gene were analyzed by Northern blot hybridization. The activity of the gene expression was found to be similar during 8~35 days of cultivation. The level of the glycolate oxidase mRNA was high in leaves, low in stems and little in roots, indicating that the expression of the gene is tissue-dependent. The mRNA level was considerably reduced when the plants were grown in dark. However, the level was increased when the dark-grown plants were subjected to illumination. These results show that light serves as a signal for the activation of the glycolate oxidase gene expression.
박양서,최정도,조남정 ( Yang Seo Park,Jung Do Choi,Nam Jeong Cho ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.3
The nature of glycolate oxidase gene in spinach was investigated by Southern and Northern blot hybridization. Genomic DNA digested with EcoRI or HindIII exhibited multiple bands when hybridized with a spinach glycolate oxidase cDNA_ Expression patterns of the gene were analyzed by Northern blot hybridization. The activity of the gene expression was found to be similar during 8∼35 days of cultivation. The level of the glycolate oxidase mRNA was high in leaves, low in stems and little in roots, indicating that the expression of the gene is tissue-dependent. The mRNA level was considerably reduced when the plants were grown in dark. However, the level was increased when the dark-grown plants were subjected to illumination. These results show that light serves as a signal for the activation of the glycolate oxidase gene expression.