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Ethionine이 흰쥐肝臟 및 血淸中의 LDH isozyme에 미치는 影響
洪思澳,尹水弘 成均館大學校 1973 論文集 Vol.18 No.-
A series of experiment has been conducted in the state of hepatiasation caused from the intraperitoneal injection of ethionine into Albino rat, in which the variation of LDH isozyme in the serum and liver have been compared in separation by the method of cellulose acetate electrophoresis, the activities of LDH and LDH isozyme have been measured by colormetric method. And it's results are follows; 1. The activities of liver and serum LDH and change of its isozyme pattern was sutdied in fasting rats given single injection of large doses of ethionine. 2. In initial periodes of experiment, both groups of control and ethionine injected rats was found 5 bands of isozyme pattern LDH_1∼LDH_5 is serum and 3 bands of isozyme pattern LDH_5, LDH_4 and LDH_2 in liver tissues. 3. The changes of liver LDH isozyme pattern was found at 48 hours after ethionine injected, that is LDH_4 was surely decreased and LDH_3 was appeared newly. 4. The activities of liver LDH in the group of ethionine injected rats was slightly decreased at 24 hours, and then again increased from 48 hours to 96 hours after ethionine injected.
洪思澳,羅圭煥,金京子,趙顯英 同德女子大學校 1969 同大論叢 Vol.1 No.1
1. In the seperation procedure of several bile acids by paper chromatography, such stationary phases as 70~80% of acetic acid and 30~50% of formic acid was found better. And in the developing solvent, n-propyl alcohol : acetic acid : H₂O(90:2:8) was found better than Krichevsky's solvent, n-propyl alcohol : ammonia : H₂O. 2. In the seperation procedure of bile acid by T. L. C. such first developing solvent as iso-amyl alcohol : formic acid : H₂O(18:5:3) was found better than iso-amyl alcohol : acetic acid : H₂O(18:5:3) And such second developing solvent as toluene acetic acid : H₂O(5:3:1) was found better in seperating desoxycholic acid and lithocholic acid both of which have the similar Rf value each other. 3. Amount of cholic acid seperated in paper chromatography was directly determind by densitometer. 4. In quantity analysis by spectrophotofluorometer when bile acid was heated at 100℃ in 98% of sulfuric acid for 10 minutes fluorescence strength was uniform. Activating wave : 430mμ.