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Morphological Changes of the Dopaminergic Network in the Rat Retina after Axotomy
천명훈,이은진,오수자,김현주 대한해부학회 2005 Anatomy & Cell Biology Vol.38 No.2
In the retina, dopaminergic cells express the receptor for brain-derived neurotrophic factor(BDNF),which is known to be retrogradely transported from higher center to the retina. This study was conducted to identifythe effect of optic nerve transaction on the dopaminergic cells in the rat retina by immunocytochemistry using anti-tyrosine hydroxylase(TH) antiserum. In the control retina, we found two types of TH-type I and type II, in the inner nuclear layer(INL) adjacent to the inner plexiform layer(IPL). The type I amacrine cellvaricosities formed ring-like structures in contact with AII amacrine cell somata in stratum 1 of the IPL. In theaxotomized retinas, TH-labeled processes formed loose networks of fibers, unlike the dense networks in the controlretina, and the ring-like structures were disrupted. Our data suggest that retrogradely transported neurotrophic factordopaminergic system. 망막에서 도파민을 함유하는 도파민성 무축삭세포 (Dopaminergic amacrine cell)는 brain-derived neurotrophic factor (BDNF)의 수용체를 발현한다. 이는 상위체계에서 망막으로 역행하여 이동된다. 본 연구에서는 흰쥐 망막에서 시각신경 절단 후 면역세포화학법을 이용하여 도파민성 무축삭세포의 표지물질인 TH의 발현변화에 대해 알아보고자 한다. 정상망막에서 TH 면역반응성은 두 종류의 도파민성 무축삭세포의 세포체에 존재하며, 이들의 세포돌기는 속핵층(inner nuclear layer) 가까이의 속얼기층(inner plexiform layer)에 주로 분지하였다. TH 면역반응성 무축삭세포 중 첫 번째 세포는 속얼기층의 1층에 주로 세포돌기와 세포염주(varicosities)를 뻗고 있으며, 이는 원형구조를 형성하며 AII 무축삭세포의 세포체와 연접을 이룬다. 시각신경 절단시 정상망막과 달리 TH로 염색된 세포돌기가 흐트러진 망상구조를 형성하는 것을 볼 수 있었고 원형구조를 형성하는 세포염주가 분열되는 것을 볼 수 있었다. 이상의 결과로 역행하여 이동되는 신경영양인자(neurotrophic factor)는 시각신경이 절단된 망막에서 TH 발현에 영향을 주며, 망막의 도파민성계를 조절할 것으로 생각된다.
집토끼 결장 각 부위의 구조에 대한 광학 및 주사전자현미경적 관찰
정진웅,천명훈,오수자,권흥식,Chung, J.W.,Chun, M.H.,Oh, S.J.,Kwun, H.S. 한국현미경학회 1984 Applied microscopy Vol.14 No.2
Structural differences in various divisions of the rabbit colon were investigated using light and scanning electron microscopy. For light microscopic study, various Portions of the colon from seven rabbits (2.5 kg body weight) were fixed in 10% neutral buffered formalin, and paraffin sections were stained with hematoxylin-eosin. Tissues for scanning electron microscopy were fixed in 1% glutaraldehyde-1.5% paraformaldehyde and postfixed in 1% $OsO_4$, dehydrated to 100% alcohol, transfered to isoamilacetate and dried by the critical point method. Subsequently, specimens were coated with gold and viewed with a JSM-35C scanning electron microscope. The colon displays a morphological diversity along its proximo-distal axis. Five regions can be discerned based on the macroscopic and microscopic characteristics. 1) The first segment immediately distal to the cecocolical junction possessing three teniae is approximately 5 cm ($4{\sim}6cm$) in length, and displays irregular folds of the mucosa oriented transversely similar to those of the cecum. 2) The second segment possessing three teniae is about 7 cm ($5{\sim}8cm$) in length, and is characterized by the papilla-like protrusions on the mucosal surface. 3) The third segment, possessing a single tenia is about 16 cm ($12{\sim}20cm$) in length, and also displays the papilla-like protrusions similar to the aforegoing segment. 4) Fusus coli, approximately 4 cm ($3{\sim}5cm$) in length, is free of teniae and exhibits longitudinal folds on the mucosal surface. These four portions together constitute the proximal colon. 5) The distal colon reaches a length of about 58 cm ($53{\sim}55cm$) and shows a pattern of surface irregularities with minor ridges on the mucosal folds.