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İbrahim Bozgeyik,Mehmet Şenel,Emre Çevik,M. Fatih Abasıyanık 한국물리학회 2011 Current Applied Physics Vol.11 No.4
An amperometric biosensor has been developed for the measurement of urea. To construct the proposed biosensor urease enzyme was immobilized onto poly(N-glycidylpyrrole-co-pyrrole) conducting film by direct covalent attachment. The biosensor surface was characterized by FTIR spectroscopy and atomic force microscopy (AFM). The response studies were carried out as a function of urea concentration with amperometric measurements. The biosensor showed a linear current response to the urea concentration ranging from 0.1 to 0.7 mM. The urea biosensor exhibited a sensitivity of 4.5 μA/mM with a response time of 4 s. The factors influencing on the performance of the resulting biosensor were also studied in detail.