TRISS Method와 ASCOT Method를 이용한 외상환자의 생존율 분석

김형수,배성만,양혁준,박철완,이근,고영관 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

Despite traumatic injuries cause serious problems in our society, there are few adequate and objective scoring system that assess the severtiy of trauma patients. The Major Trauma Outcome Study(MTOS)in United States deisgned the TRISS(Trauma Score & Injury Severity Score)method by means of Revised Trauma Score(RTS),Injury Severity Score(ISS0,and age,while Champion et al deviced ASCOT(A Severtriy Characterizaiton Of Trauma)method to overcome the limitation of TRISS. This study attempted to make a comparison between TRISS and ASCOT by using date for 422 injured patients which were collected form September,1993 to February,1994. Ascot and TRISS were compared in their sensutivity,Specificity,disparity and Z-statistics. 1)Sex ratio of male to female was 2.8:1 and the commonest age of trauma patients was thirties (23.8%). 2)The average probability of survival(Ps)for 442 patient by TRISS method was 0.9228 and that of ASCOT method 0.9356. 3)Disparity of Ps between survival and non-survival using TRISS and ASCOT was relatively low for both indexes as 0.3507 and 0.3296, respectively. 4)The sensitivity rates(number of patients predicted to die who actually died/total who actually died)for the non-survival of both TRISS and ASCOT method were low (35.0%),but the specificity rates(number of patients predicted to live who actually lived/total who actually lived)for the survival of TRISS and ASCOT were 99.1%and 99.6%respectively. 5)Z-statistics(difference between predicted and actual number of death)of both TRISS(1.3224)and ASCOT(1.2234)resulted in positive value which meant that actual number of death exeeded predicted number of death. 6)The ASCOT that have presumed to be more accurate method for patients with head trauma and with multiple injuries to one portion of body,had its intricacy and difficult points in practical application. And the difference between the result of ASCOT and TRISS was not so significant. 7)It is thought that a new, more comprehensive index would like to be developed and thoroughly tested on a variety of data sets in order for it to be used in trauma system quality assurance evaluation.

A Hierarchical Phosphorylation Cascade That Regulates the Timing of PERIOD Nuclear Entry Reveals Novel Roles for Proline-Directed Kinases and GSK-3{beta}/SGG in Circadian Clocks.

Ko, Hyuk Wan,Kim, Eun Young,Chiu, Joanna,Vanselow, Jens T,Kramer, Achim,Edery, Isaac The Society 2010 The Journal of neuroscience Vol.30 No.38

<P>The daily timing of when PERIOD (PER) proteins translocate from the cytoplasm to the nucleus is a critical step in clock mechanisms underpinning circadian rhythms in animals. Numerous lines of evidence indicate that phosphorylation plays a prominent role in regulating various aspects of PER function and metabolism, including changes in its daily stability and subcellular distribution. In this report, we show that phosphorylation of serine 661 (Ser661) by a proline-directed kinase(s) is a key phospho-signal on the Drosophila PER protein (dPER) that regulates the timing of its nuclear accumulation. Mutations that block phosphorylation at Ser661 do not affect dPER stability but delay its nuclear entry in key pacemaker neurons, yielding longer behavioral rhythms. Intriguingly, abolishing phosphorylation at Ser661 also attenuates the extent of dPER hyperphosphorylation in vivo, suggesting the phosphorylated state of Ser661 regulates phosphorylation at other sites on dPER. Indeed, we identify Ser657 as a site that is phosphorylated by the glycogen synthase kinase GSK-3관 (SHAGGY; SGG) in a manner dependent on priming at Ser661. Although not as dramatic as mutating Ser661, mutations that abolish phosphorylation at Ser657 also lead to longer behavioral periods, suggesting that a multi-kinase hierarchical phosphorylation module regulates the timing of dPER nuclear entry. Together with evidence in mammalian systems, our findings implicate proline-directed kinases in clock mechanisms and suggest that PER proteins are key downstream targets of lithium therapy, a potent inhibitor of GSK-3관 used to treat manic depression, a disorder associated with clock malfunction in humans.</P>

Invited Mini Review : The primary cilium as a multiple cellular signaling scaffold in development and disease

( Hyuk Wan Ko ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.8

Primary cilia, single hair-like appendage on the surface of the most mammalian cells, were once considered to be vestigial cellular organelles for a past century because of their tiny structure and unknown function. Although they lack ancestral motility function of cilia or flagella, they share common ground with multiciliated motile cilia and flagella on internal structure such as microtubule based nine outer doublets nucleated from the base of mother centrioles called basal body. Making cilia, ciliogenesis, in cells depends on the cell cycle stage due to reuse of centrioles for cell division forming mitotic spindle pole (M phase) and assembling cilia from basal body (starting G1 phase and maintaining most of interphase). Ciliary assembly required two conflicting processes such as assembly and disassembly and balance between these two processes determines the length of cilia. Both process required highly conserved transport system to supply needed substance to grow tip of cilia and bring ciliary turnover product back to the base of cilia using motor protein, kinesin and dynein, and transport protein complex, IFT particles. Disruption of ciliary structure or function causes multiple human disorder called ciliopathies affecting disease of diverse ciliated tissues ranging from eye, kidney, respiratory tract and brain. Recent explosion of research on the primary cilia and their involvement on animal development and disease attracts scientific interest on how extensively the function of cilia related to specific cell physiology and signaling pathway. In this review, I introduce general features of primary cilia and recent progress in understanding of the ciliary length control and signaling pathways transduced through primary cilia in vertebrates. [BMB Reports 2012; 45(8): 427-432]

Tissue-specific ciliary function in mouse development

Hyuk Wan Ko 한국실험동물학회 2018 한국실험동물학회 학술발표대회 논문집 Vol.2018 No.7

Anthraquinone and Stibene Derivatives from the Cultivated Korean Rubbarb Rhizomes

Ko, Sung-Kwon,Whang, Wan-Kyunn,Kim, Il-Hyuk The Pharmaceutical Society of Korea 1995 Archives of Pharmacal Research Vol.18 No.4

The sutdies were carried out to evaluate the consituents in the rihzomes of the cultivated Korean Rhubarb (Polygonaceae). From the acetone fraction ofl methanol extract Compound I (1, 8-dihydroxy-3-methyl anthraquinone, chrysophanic acid), Compound II($chrysophanol-8-O-{\beta}-D-glucopyranoside$), Compound III ($emodin-8-O-{\beta}-D-glucopyranoside$) and Compound IV ($aloe-emodin-8-O-{\beta}-D-glucopyranoside$), and from the ether fraction Compound V(1, 8-dihydorxy-3-emthyl-6-methoxy anthraquinone, physcion) and Compound VI (1, 6, 8-thitydroxy-3-emthyl anthrauinone, emodin), and also from the n-buthanol fraction Compound VII ($rhapontigenin-3-O-{\beta}-D-glucopyranoside, rhaponticin$) and Compound VIII ($piceatannol-3'-O-{\beta}-D-glucopyranoside$), were isolated and identified on the basis of their physico-chemical and spectroscopic evidences (UV, IR, H-NMR, C-NMR, EI-MS), respectively.

인삼류 생약의 항당뇨에 관한 비교연구 : 인삼류 엑스가 Streptozotocin유발 고혈당 흰쥐에 미치는 영향 The Effects of Ginseng Radices Extracts on Streptozotocin-induced Hyperglycemic in Rats

고성권,황완균,김일혁 중앙대학교 약학연구소 1993 약학 논총 Vol.7 No.-

This study was attempted to compare the antidiabetic activities of Ginseng Radices on the hyperglycemia induced by Streptozotocin in rats. The buthanol fractions of roots of Ginseng Radices were administered before and after injection of streptozotocin (50mg/kg,i.p.), and then blood glucose, serum cholesterol, and serum triglyceride levels were determined at 96 hours after streptozotocin injection. 1. The blood glucose levels in streptozotocin-induced hyperglycemic rats were dose-dependently decreased by the administration of buthanol frations of the Ginseng Radices. The administration of the buthanol frations(400mg/kg,P.O.) of Ginseng Radix alba and Ginseng Radix palva were significantly shown to decreased blood glucose level comparing with streptozotocin control group. 2. The serum total cholesterol levels in streptozotocin-induced hyperglycemic rats were significantly decreased by the administrations of the buthanol fractions(400mg/kg,P.O.) of Ginseng Radix aquosa and Ginseng Radix alba and Ginseng Radix palva. But the buthanol fraction (200mg/kg,P.O.) of Ginseng Radix alba and the buthanol fractions (100,200,400mg/kg) of Ginseng Radix rubra were slightly decreased. 3. The serum triglyceride levels of streptozotocin-induced hyperglycemic rats were significantly decreased by the administration all of the four Ginseng Radices (100,200,400mg/kg,p.o.).

연소성 류마티스 관절염에 병발된 간질성 폐질환

고혁 ( Hyuk Ko ),최수정 ( Soo Jung Choi ),장현규 ( Hyun Kyu Chang ),김종완 ( Jong Wan Kim ),김승렬 ( Seong Reol Kim ) 대한류마티스학회 2002 대한류마티스학회지 Vol.9 No.3

Juvenile rheumatoid arthritis (JRA) can develop the extra-articular manifestations, which are growth retardation, osteopenia, chronic uveitis, and rarely pleuropulmonary involvement. Approximately 30% of patients with JRA show abnormal pulmonary function test without pulmonary symptoms, in which the frequent abnormality is in carbon monoxide diffusing capacity, but clinically evident pulmonary parenchymal disease in JRA is extremely uncommon. We describe a 23-years-old female with JRA who presented with dyspnea due to interstitial lung disease and pulmonary hypertension. She had suffered from polyarthralgia and dyspnea since 8 years old.

좀 갈매나무 줄기의 플라보노이드

황완균(Wan Kyunn Whang),정혜진(Hyeh Jean Jeong),고성권(Sung Kwon Ko),이무택(Moo Taek Lee),김일혁(Il Hyuk Kim) 대한약학회 1996 약학회지 Vol.40 No.4

As a series of the studies on the specific and indigenous plants of Mt. Halla, the constituents from stem of Rhamnus taquetii (Rhamnaceae) were investigated. From the water fraction of the MeOH extract, a new flavonol glycoside, kaempferide-3-O-alpha-L-rhamnopyranosyl(1->3)-alpha-L-rhamnopyranosyl(1-6)-beta-D-galactopyranoside, was isolated with three known compounds, quercetin, quercetin-3-methylether, kaempferol, by column chromatographic separation using Amberlite XAD-2, ODS-gel and Sephadex LH-20 and elucidated physico-chemical evidences(1H-NMR, 13C-NMR,IR,EI-Mass,FAB-Mass, and G.C.), respectively.

제주도내 자연발생한 말유산태아에서 PCR을 이용한 Equine Herpesvirus Type-1 검출

문혁 ( Hyuk Moon ),강완철 ( Wan Cheul Kang ),김은주 ( Eun Joo Kim ),김진회 ( Jin Hoe Kim ),고현주 ( Hyun Joo Ko ),양재혁 ( Jae Hyuk Yang ),손원근 ( Won Geun Son ),이두식 ( Du Sik Lee ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.1

It is impotent to identify the causative agents of abortion in equine for minimizing the loss of breeding costs in equine industry. Recently, the abortion has often occurred in equine herds and thus the purpose of the study was aimed at the identification of equine herpesvirus-1, one of the frequent pathogens to abortion, using polymerase chain reaction. Six fetuses to be aborted at nine to ten months in pregnancy reared in six herds were used in the study. Two primers in the PCR were made from glycoprotein B gene of EHV-1. The primers specific for EHV-1 amplified 1880 bp of PCR products from DNA extracts from thorax fluids, livers, lungs, and spleens of four in six aborted fetuses. Consequently, PCR could be applied to diagnose the abortion of EHV-1 and also confirmed to play a major role of the viral pathogen associated with equine abortion in Jeju island.

중독 중환자에서의 초기 영양상태평가와 예후와의 관계

고동완 ( Dong-wan Ko ),최상천 ( Sangcheon Choi ),민영기 ( Young-gi Min ),이혁진 ( Hyuk Jin Lee ),박은정 ( Eun Jung Park ) 대한임상독성학회 2021 대한임상독성학회지 Vol.19 No.2

Purpose: Nutritional status and support in critically ill patients are important factors in determining patient recovery and prognosis. The aim of this study was to analyze the early nutritional status and the methods of nutritional support in critically ill patients with acute poisoning and to evaluate the effect of nutritional status on prognosis. Methods: A retrospective study was conducted in tertiary care teaching hospital from January 2018 to December 2020. in an emergency department of university hospital, 220 patients who were stayed more than 2 days of poisoning in intensive care unit were enrolled. Results: 155 (70.5%) of patients with acute poisoning had low-risk in nutritional risk screening (NRS). Patients with malignancy had higher NRS (low risk 5.2%, moderate risk 18.5%, high risk 13.2%, p=0.024). Patients of 91.4% supplied nutrition via oral route or enteral route. Parenteral route for starting method of nutritional support were higher in patients with acute poisoning of herbicide or pesticide (medicine 3.2%, herbicide 13.8%, pesticide 22.2%, p=0.000). In multivariate logistic regression analysis, herbicide or pesticide intoxication, higher risk in NRS and sequential organ failure assessment over 4.5 were affecting factor on poor recovery at discharge. Conclusion: NRS in patients intoxicated with herbicide or pesticide were higher than that in patients intoxicated with medicine intoxication. Enteral nutrition in patients intoxicated with herbicide or pesticide was less common. Initial NRS was correlated with recovery at discharge in patient with intoxication. It is expected to be helpful in finding patients with high-risk nutritional status in acute poisoning patients and establishing a treatment plan that can actively implement nutritional support.