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RISS 인기검색어
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- 저자 : (Cady Engler (검색결과 4 건)
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Kinetic Models for Growth and Product Formation on Multiple Substrates
권윤중,Cady R. Engler 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.6
Hydrolyzates from lignocellulosic biomass contain a mixture of simple sugars; the predominant ones being glucose, cellobiose and xylose. The fermentation of such mixtures to ethanol or other chemicals requires an understanding of how each of these substrates is utilized. Candida lusitaniae can efficiently produce ethanol from both glucose and cellobiose and is an attractive organism for ethanol production. Experiments were performed to obtain kinetic data for ethanol production from glucose, cellobiose and xylose. Various combinations were tested in order to determine kinetic behavior with multiple carbon sources. Glucose was shown to repress the utilization of cellobiose and xylose. However, cellobiose and xylose were simultaneously utilized after glucose depletion. Maximum volumetric ethanol production rates were 0.56, 0.33, and 0.003 g/L·h from glucose, cellobiose and xylose, respectively. A kinetic model based on cAMP mediated catabolite repression was developed. This model adequately described the growth and ethanol production from a mixture of sugars in a batch culture.
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Kinetic Models for Growth and Product Formation on Multiple Substrates
Kwon, Yun-Joong,Engler, Cady R. The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.6
Hydrolyzates from lignocellulosic biomass contain a mixture of simple sugars; the predominant ones being glucose, cellobiose and xylose. The fermentation of such mixtures to ethanol or other chemicals requires an understanding of how each of these substrates is utilized. Candida lusitaniae can efficiently produce ethanol from both glucose and cellobiose and is an attractive organism for ethanol production. Experiments were performed to obtain kinetic data for ethanol production from glucose, cellobiose and xylose. Various combinations were tested in order to determine kinetic behavior with multiple carbon sources. Glucose was shown to repress the utilization of cellobiose and xylose. However, cellobiose and xylose were simultaneously utilized after glucose depletion. Maximum volumetric ethanol production rates were 0.56, 0.33, and 0.003 g/L h from glucose, cellobiose and xylose, respectively. A kinetic model based on cAMP mediated catabolite repression was developed. This model adequately described the growth and ethanol production from a mixture of sugars in a batch culture.
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(Kwon Bok Lee,(Cady Engler,(Jae E . Yang,(Shin Woo Lee,(Yong Ha Park 한국응용생명화학회 2001 Journal of Applied Biological Chemistry (J. Appl. Vol.44 No.2
Capsaicin synthesis by suspension cultured cells of Jalapeno pepper (Capcicum annuum L.) was assessed in vitro under various conditions including temperature (23 and 30℃), light intensity (with light and without light), and shaking speed (110 and 200 rpm).
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Stack,James P.,Engler,Cady R.,Albert,Carcia Ⅲ,Koh,Jeong-Sam 濟州大學校 亞熱帶農業硏究所 1990 亞熱帶農業硏究 Vol.7 No.-
땅콩에서 分離한 곰팡이를 사용하여 免疫抑制活性을 갖는 抗菌性代謝産物의 生産을 위한 振揚培地條件을 檢討하였다. 耐熱性인 이 菌을 Penicillium citrinum var Pa-6으로 同定하였으며, 窒素源을 制限한 培地에서 35℃, 2주간 培養에서 最大의 抗菌作用을 나타냈다. 最適培地組成을 檢査한 結果, 1.5% 칼락토스와 0.5% 포도당을 炭素源으로 하여 C/N비율이 50이 되도록 0.046% NH₄NO₃를 窒素源으로 添加하고, 10mM 無機隣酸과 無機鹽類를 少量 첨가한 培地였다. 또한 페닐알라닌, 글루타민 또는 타이로신과 같은 몇 종류의 아미노산을 添加함으로써 抗菌作用은 배양중에 빨리 나타남을 알 수 있었다. Cultural conditions of shake-flask culture for the production of antimicrobial metabolites containing immunosuppressive activities by fungi isolated from peanut seeds were investigated. The thermotolerant stain, identified as Penicillium citrinum var Pa-6, produced maximum antimicrobial metabolites at 35℃ after 2 weeks on nitrogen-limiting medium. According to cultural conditions, optimum medium compositions for antimicrobial metabolites production were defind as follws; 1.5% of galactose with 0.5% dextrose, 0.046$ NH₄NO₃(C/N ratio 50), 10mM of Pi and trace elements. The expression of antimicrobial actvities were appeared sooner than control during cultivation with the addition of some amino acid such as phenylalanine, glutamine or tyrosine.
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