POSTERS / PW-015 : DEVELOPMENT OF BIOASSAY SYSTEM TO IDENTIFY ENDOCRINE DISRUPTORS

(You A . Hwang),(Kum Joo Shin),(Pann Ghill Suh),(Sung Ho Ryu) 한국생화학분자생물학회 (구 한국생화학회) 1999 6th IUBMB SEOUL CONFERENCE Vol.- No.-

Effects of substance P on mineralization markers and heme oxygenase-1 Expression in human immortalized periodontal ligament cells

You-Min Cho,Chung-Hwan Suh,Sang Woo Chun,Eun-Cheol Kim,Kyung-Hwa Kang KOREAN ACADAMY OF ORAL BIOLOGY 2008 International Journal of Oral Biology Vol.33 No.4

Substance P (SP) is known to be expressed in the nerve fibers of dental pulp and periodontal tissues. It was recently reported that SP expression increased in response to orthodontic force. In the present study, we investigated the effect of SP on expression of mineralization markers and heme oxygenase-1 (HO-1) in human immortalized periodontal ligament (IPDL) cells. Cell viability was measured using a 3,4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) assay. The expression of mineralization markers, including alkaline phosphatase (ALP), osteonectin (ON) and bone sialoprotein (BSP), and heme oxygenase-1 (HO-1) was assessed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. SP did not significantly change human IPDL cell viability, with the exception of the 24 hour treatment group. Treatment of human IPDL cells with 10-10 to 10-4 M SP upregulated mineralization marker and HO-1 expression in a time- and concentration-dependent manner. Our results suggest that SP may modulate osteoblastic cell differentiation of human IPDL cells through a mechanism involving HO-1 expression.

Hip Fracture Surgery without Transfusion in Patients with Hemoglobin Less Than 10 g/dL

You-Sung Suh,Jae-Hwi Nho,Jonghyeon Seo,Byung-Woong Jang,Jong-Seok Park 대한정형외과학회 2021 Clinics in Orthopedic Surgery Vol.13 No.1

Background: Hip fracture surgery is associated with blood loss, which may lead to adverse patient outcomes. The hemoglobin level declines gradually in most hip fracture cases involving femoral neck fractures and intertrochanteric fractures. It decreases further after hip fracture surgery due to perioperative bleeding. We developed a protocol, which avoids transfusion in hip fracture surgery, and reviewed the hemodynamic outcomes of patients with hemoglobin less than 10 g/dL without transfusion. Methods: From 2014 to 2019, we retrospectively recruited 34 patients with hip fractures and a hemoglobin level less than 10 g/dL, who refused to undergo transfusion. There were 19 patients with femoral neck fractures and 15 patients with intertrochanteric fractures. Our patient blood management (PBM) protocol involving 4,000 U erythropoietin (3 times a week) and 100 mg iron supplement (every day) was applied to all included patients. Intraoperatively, a cell saver and tranexamic acid were used. Postoperatively, the protocol was maintained until the patients’ hemoglobin level reached 10 g/dL. We evaluated the feasibility of our protocol, perioperative complications, and hemodynamic changes. Results: Nineteen patients with femoral neck fractures underwent bipolar hemiarthroplasty and 15 patients with intertrochanteric fractures underwent internal fixation with a cephalomedullary nail. The mean hemoglobin level was 8.9 g/dL (range, 7.3–9.9 g/dL) preoperatively, 7.9 g/dL (range, 6.5–9.3 g/dL) immediately postoperatively, 7.7 g/dL (range, 4.3–9.5 g/dL) on postoperative day 1, 7.4 g/dL (range, 4.2–9.4 g/dL) on postoperative day 3, 8.1 g/dL (range, 4.4–9.7 g/dL) on postoperative day 5, 8.5 g/dL (range, 4.5–9.9 g/dL) on postoperative day 7, and 9.9 g/dL (range, 5.7–11.1 g/dL) on postoperative day 14. The average intraoperative bleeding was 206.2 ± 78.7 mL. There was no case associated with complications of anemia. Conclusions: Hip fracture surgery in patients with hemoglobin less than 10 g/dL was feasible without the need for transfusion using our PBM protocol in 34 patients. Using this protocol, the operation was conducted safely despite the anemic condition of patients with fractures whose hemoglobin was less than 10 g/dL.

Remote Cerebral and Cerebellar Hemorrhage after Massive Cerebrospinal Fluid Leakage

You, Sung-Hye,Son, Kyu-Ri,Lee, Nam-Joon,Suh, Jung-Keun The Korean Neurosurgical Society 2012 Journal of Korean neurosurgical society Vol.51 No.4

Dural tears can occur during spinal surgery and may lead to cerebrospinal fluid (CSF) leakage which is rarely involved in remote cerebellar hemorrhage. Only a few of cases of simultaneous cerebral and cerebellar hemorrhage have been reported in the English literature. We experienced a case of multiple remote cerebral and cerebellar hemorrhages in a 63-year-old man who exhibited no significant neurologic deficits after spinal surgery. Magnetic resonance imaging (MRI) performed 4 days after the surgery showed a large amount of CSF leakage in the lumbosacral space. The patient underwent the second surgery for primary repair of the dural defect, but complained of headache after dural repair surgery. Brain MRI taken 6 days after the dural repair surgery revealed multifocal remote intracerebral and cerebellar hemorrhages in the right temporal lobe and both cerebellar hemispheres. We recommend diagnostic imaging to secure early identification and treatment of this complication in order to prevent serious neurologic deficits.

Cutaneous sporotrichosis of the upper eyelid in an adult

( You Bum Song ),( Sang Youl Yun ),( Moo Kyu Suh ),( Gyoung Yim Ha ),( Jung Ran Kim ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1

Sporotrichosis is a chronic infectious disease caused by Sporothrix(S.) schenckii. Fixed form of sporotrichosis is rare than lymphocutaneous form. Initial cutaneous lesion is most frequently on an exposed area, commonly the face in children and the upper extremity in adults. We report the fixed cutaneous sporotrichosis in a 57-year-old women. The lesions were manifested by erythematous plaques with swelling on the right upper eyelid for 1 month. The fungal culture from biopsy specimen on Sabouraud``s dextrose agar showed dark brown to black, moist and wrinkled colonies of S. schenckii. Histopathologically, pseudoepitheliomatous hyperplasia and chronic granulomatous inflammation were observed on H & E stain. Septate branched mycelia and clustered conidia were observed in slide culture. The nucleotide sequence of internal transcribed spacer for clinical isolate was identical to that of S. schenckii strain KMU 3360(GenBank accession number AB122043.1). The patient was treated with 200mg of oral itraconazole daily for 6 months. The skin lesions were completely cured and recurrence is not observed to date.

Hemodynamic management of septic shock: Beyond of the SSC guidelines

Suh Gil Joon,Shin Tae Gun,Kwon Woon Yong,Kim Kyuseok,Jo You Hwan,최성혁,Chung Sung Phil,Kim Won Young 대한응급의학회 2023 Clinical and Experimental Emergency Medicine Vol.10 No.3

Although the Surviving Sepsis Campaign guidelines provide standardized and generalized guidance, they are less individualized. This review focuses on recent updates in the hemodynamic management of septic shock. Monitoring and intervention for septic shock should be personalized according to the phase of shock. In the salvage phase, fluid resuscitation and vasopressors should be given to provide life-saving tissue perfusion. During the optimization phase, tissue perfusion should be optimized. In the stabilization and de-escalation phases, minimal fluid infusion and safe fluid removal should be performed, respectively, while preserving organ perfusion. There is controversy surrounding the use of restrictive versus liberal fluid strategies after initial resuscitation. Fluid administration after initial resuscitation should depend upon the patient’s fluid responsiveness and requires individualized management. A number of dynamic tests have been proposed to monitor fluid responsiveness, which can help clinicians decide whether to give fluid or not. The optimal timing for the initiation of vasopressor agents is unknown. Recent data suggest that early vasopressor initiation should be considered. Inotropes can be considered in patients with decreased cardiac contractility associated with impaired tissue perfusion despite adequate volume status and arterial blood pressure. Venoarterial extracorporeal membrane oxygenation should be considered for refractory septic shock with severe cardiac systolic dysfunction.

Antifungal susceptibility testing using etest of candida species isolated from patients with oral candidiasis

( You Bum Song ),( Jun Gyu Song ),( Sang Youl Yun ),( Moo Kyu Suh ),( Gyoung Yim Ha ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: The necessity of performing antifungal susceptibility tests is recently increasing, because of frequent cases of oral candidiasis caused by antifungal-resistant Candida species. The Etest (BioMeriux, Marcy l``Etoile, France) is a rapid, easy-to-perform in-vitro antifungal susceptibility test. Objectives: The purpose of this study was to determine the minimal inhibitory concentrations (MICs) of antifungal agents using the Etest for Candida species isolated from patients with oral candidiasis Methods: Forty-seven clinical isolates of Candida species were tested along with a reference strain. MIC endpoints of Etest for fluconazole, itraconazole, voriconazole, amphotericin B susceptibility were read after 24 hour incubation of each isolate on RPMI 1640 agar. Results: MICs of fluconazole were 0.064-0.75 レg/mL for C. albicans, 16-64 レg/mL for C. glabrata, and 0.25-0.75レg/mL for C. tropicalis. MICs of itraconazole were 0.002-0.094 レg/mL for C. albicans, over 32レg/mL for C. glabrata, and 0.012-0.25 レg/mL for C. tropicalis. MICs of voriconazole were 0.002-0.016 レg/mL for C. albicans, 0.38-1.0 レg/mL for C. glabrata, and 0.008-0.094 レg/mL for C. tropicalis. MICs of amphotericin B were 0.012-0.19レg/mL for C. albicans, 0.064-0.19 レg/mL for C. glabrata, and 0.094-0.25 レg/mL for C. tropicalis. Conclusion: This study revealed that the Etest represented a simple and efficacious method for antifungal susceptibility testing of Candida species isolated from oral candidiasis patients.

Poster Session 4 : Immunology ; Engineering N-glycosylation mutations in IL-12 enhances sustained cytotoxic T Lymphocyte responses for DNA immunization

( Sang Jun Ha ),( Jun Chang ),( Man Ki Song ),( You Seok Suh ),( Hyun Tak Jin ),( Chu Hee Lee ),( Gyu Hyun Nam ),( Gil Don Choi ),( Kwan Yong Choi ),( Sung Hee Lee ),( Won Bae Kim ),( Young Chul Sung 한국생화학분자생물학회 (구 한국생화학회) 2003 생화학분자생물학회 춘계학술발표논문집 Vol.2003 No.-

Effects of Medicinal herb Extracts and their Components on Steatogenic Hepatotoxicity in Sk-hep1 Cells

You-Jin Choi,Yujin Yoon,Ho-Sung Choi,Sora Park,Sehee Oh,Se-Mi Jeong,Hyo-Ryung Suh,Byung-Hoon Lee 한국독성학회 2011 Toxicological Research Vol.27 No.4

Herbal medicines are widely used in many countries for the treatment of many diseases. Although the use of herb extracts as alternative medicine is growing, their toxicological properties have not been thoroughly investigated. In this study, we have investigated the effects of water and ethanol extracts of 18 herbs on the hepatic lipid metabolism and steatogenic hepatotoxicity. Ethanol extracts of Cirsium japonicum, Carthamus tinctorius, Rehmanniae glutinosa (preparata), Polygala tenuifolia, Foeniculum vulgare, Polygonum multiflorum, and Acorus gramineus and water extracts of Polygonum multiflorum and Rehmanniae glutinosa induced lipid accumulation in Sk-hep1 human hepatoma cells as determined by Nile red staining. These extracts increased the luciferase activity of sterol regulatory element (SRE) and decreased that of peroxisome proliferator response element (PPRE), indicating the possibilities of enhanced fatty acid synthesis and decreased fatty acid oxidation. To identify the components responsible for the fat accumulation, we tested 50 chemicals isolated from the nine herbs. Apigenin, luteolin, pectolinarin and lupeol from Cirsium japonicum, 8-methoxypsoralen and umbelliferone from Foeniculum vulgare and pomonic acid and jiocerebroside from Rehmanniae glutinosa significantly increased the accumulation of lipid droplets. These results suggest that ethanol extracts of Cirsium japonicum, Carthamus tinctorius, Rehmanniae glutinosa (preparata), Polygala tenuifolia, Foeniculum vulgare, Polygonum multiflorum, and Acorus gramineus and water extracts of Polygonum multiflorum and Rehmanniae glutinosa can cause fatty liver disease by decreasing β-oxidation of fatty acid and increasing lipogenesis.

Clinical and Radiologic Outcomes among Bipolar Hemiarthroplasty, Compression Hip Screw and Proximal Femur Nail Antirotation in Treating Comminuted Intertrochanteric Fractures

( You Sung Suh ),( Jae Hwi Nho ),( Seong Min Kim ),( Sijohn Hong ),( Hyung Suk Choi ),( Jong Seok Park ) 대한고관절학회 2015 Hip and Pelvis Vol.27 No.1

Purpose: In comminuted intertrochanteric fractures, various operative options have been introduced. The purpose of this study was to determine whether there were differences in clinical and radiologic outcomes among bipolar hemiarthroplasty (BH), compression hip screw (CHS) and proximal femur nail antirotation (PFNA) in treating comminuted intertrochanteric fractures (AO/OTA classification, A2 [22, 23]) Materials and Methods: We retrospectively evaluated total 150 patients (BH, 50; CHS, 50; PFNA, 50) who were operated due to intertrochanteric fractures from March 2010 to December 2012 and were older than 65 years at the time of surgery. We compared these three groups for radiologic and clinical outcomes at 12 months postoperatively, including Harris Hip Score, mobility (Koval stage), visual analogue scale and radiologic limb length discrepancy (shortening). Results: There was no statistical significance among three groups in clinical outcomes including Harris Hip Score, mobility (Koval stage), visual analogue scale. However, there was significant differences in radiologic limb discrepancy in plain radiographs at 12 months postoperatively (radiologic shortening: BH, 2.3 mm; CHS, 5.1 mm; PFNA, 3.0 mm; P=0.000). Conclusion: There were no clinical differences among BH, PFNA, and CHS in this study. However, notable limb length shortening could be originated during fracture healing in osteosynthesis, compared to arthroplasty (BH<PFNA<CHS).