Pathological Lesions and Inducible Nitric Oxide Synthase Expressions in the Liver of Mice Experimentally Infected with Clonorchis sinensis

Qing-Li Yang,Ji-Qing Shen,Yan Xue,Xiao-Bing Cheng,Zhi-Hua Jiang,Yi-Chao Yang,Ying-Dan Chen,Xiao-Nong Zhou 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.6

The nitric oxide (NO) formation and intrinsic nitrosation may be involved in the possible mechanisms of liver fluke-associated carcinogenesis. We still do not know much about the responses of inducible NO synthase (iNOS) induced by Clonorchis sinensis infection. This study was conducted to explore the pathological lesions and iNOS expressions in the liver of mice with different infection intensity levels of C. sinensis. Extensive periductal inflammatory cell infiltration, bile duct hyperplasia, and fibrosis were commonly observed during the infection. The different pathological responses in liver tissues strongly correlated with the infection intensity of C. sinensis. Massive acute spotty necrosis occurred in the liver parenchyma after a severe infection. The iNOS activity in liver tissues increased, and iNOS-expressing cells with morphological differences were observed after a moderate or severe infection. The iNOS-expressing cells in liver tissues had multiple origins.

Design and implementation of a SHM system for a heritage timber building

Qing-shan Yang,Juan Wang,Sunjoong Kim,Huihui Chen,Billie F. Spencer Jr 국제구조공학회 2022 Smart Structures and Systems, An International Jou Vol.29 No.4

Heritage timber structures represent the history and culture of a nation. These structures have been inherited from previous generations; however, they inevitably exhibit deterioration over time, potentially leading to structural deficiencies. Structural Health Monitoring (SHM) offers the potential to assess operational anomalies, deterioration, and damage through processing and analysis of data collected from transducers and sensors mounted on the structure. This paper reports on the design and implementation of a long-term SHM system on the Feiyun Wooden Pavilion in China, a three-story timber building built more than 500 years ago. The principles and features of the design and implementation of SHM systems for heritage timber buildings are systematically discussed. In total, 104 sensors of 6 different types are deployed on the structure to monitor the environmental effects and structural responses, including air temperature and humidity, wind speed and direction, structural temperatures, strain, inclination, and acceleration. In addition, integrated data acquisition and transmission subsystem using a newly developed software platform are implemented. Selected preliminary statistical and correlation analysis using one year of monitoring data are presented to demonstrate the condition assessment capability of the system based on the monitoring data.

Cellular and ultrastructural changes in the seedling roots of upland rice (Oryza sativa) under the stress of two allelochemicals from Ageratina adenophora

Yang, Guo-Qing,Wan, Fang-Hao,Guo, Jian-Ying,Liu, Wan-Xue The Korean Society of Weed Science and The Turfgra 2011 Weed Biology and Management Vol.11 No.3

Two sesquiterpene-derivative compounds, 4,7-dimethyl-1-(propan-2-ylidene)-1,4,4a,8atetrahydronaphthalene-2,6(1H, 7H)-dione (DTD) and 6-hydroxy-5-isopropyl-3,8-dimethyl-4a,5,6,7,8,8a-hexahydronaphthalen-2(1H)-one (HHO), are the major putative allelochemicals of the aqueous leachates of Ageratina adenophora.A laboratory experiment was conducted, using the hydroponic method, to evaluate the cellular and ultrastructural changes in the seedling roots of upland rice under the stress of DTD and HHO. The subsequent changes were observed in the treated upland rice roots in comparison with their controls. The scanning electron microscopy results showed that the DTD-treated root tip cells turned into an irregular arrangement and shape and that most of them were wizened, with a poor cytoplasm. In the HHO treatment, the root tips had many irregularly shaped cells, with a greater number of sloughing cells, as well as short, wide cells that resulted in spherical and wider, but shorter, roots. At the ultrastructural level, DTD and HHO induced irregularly shaped and lobed nuclei, increased cytoplasmic vacuolation, reduced ribosome density and dictyosomes, and a reduced number of mitochondria in the cells, which indicated limited protein transportation and a reduced capability to export substances for cell development and growth in the upland rice seedling roots. The overall effect of HHO on the upland rice seedlings was more pronounced than that of DTD.

LETTER TO THE EDITOR : Hydroxychloroquine-Induced Reversible Hypomnesis in a Patient with Reticular Erythematous Mucinosis

Qing Lin,Di Qing Luo,Jun Hua Liu,Wei Yang 대한피부과학회 2012 Annals of Dermatology Vol.24 No.4

Simultaneous Determination of Glutamate, Glycine, and Alanine in Human Plasma Using Precolumn Derivatization with 6-Aminoquinolyl- N-hydroxysuccinimidyl Carbamate and High-Performance Liquid Chromatography

Qing Zhong Li,Qing Xian Huang,Shu Cui Li,Mei Zi Yang,Bin Rao 대한생리학회-대한약리학회 2012 The Korean Journal of Physiology & Pharmacology Vol.15 No.5

A simple, sensitive and reproducible high-performance liquid chromatography (HPLC) method has been validated for determining concentrations of glutamate, glycine, and alanine in human plasma. Proteins in plasma were precipitated with perchloric acid, followed by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). Simultaneous analysis of glutamate, glycine, and alanine is achieved using reversed-phase HPLC conditions and ultraviolet detection. Excellent linearity was observed for these three amino acids over their concentration ranges with correlation coefficients (r)>0.999. The intra- and inter-day precision were below 10%. This method utilizes quality control samples and demonstrates excellent plasma recovery and accuracy. The developed method has been successfully applied to measure plasma glutamate, glycine, and alanine in twenty volunteers.

Design of potent, non-toxic anticancer peptides based on the structure of the antimicrobial peptide, temporin-1CEa

Qing-Zhu Yang,Che Wang,Lei Lang,Yang Zhou,He Wang,De-Jing Shang 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.11

Recent advances in the search for novel anticanceragents have indicated that the positively chargedantimicrobial peptides have emerged as promising agentsoffering several advantages over the conventional anticancerdrugs. As a naturally occurring, cationic, a-helicalantimicrobial peptide, temproin-1CEa has been proved toexhibit a potent anticancer effect and a moderate hemolyticactivity. In order to reduce the hemolytic activity of temporin-1CEa and improve its anticancer potency towards arange of human breast cancer cells, in the present study, sixanalogs of temporin-1CEa were rationally designed andsynthesized. The amphipathicity levels and a-helicalstructural patterns of peptides were reserved, while theircationic property and hydrophobicity were changed. Theresults of MTT and hemolysis assay indicated that theanalog peptides displayed an improved anticancer activityand showed an overall optimized therapeutic index. Thehydrophobicity of peptides was positively correlated withtheir hemolytic and antitumor activities. Moreover, the datasuggest a strategy of increasing the cationicity whilemaintaining the moderate hydrophobicity of naturallyoccurring amphipathic a-helical peptides to generate analogswith improved cytotoxicity against tumor cells butdecreased activity against non-neoplastic cells such ashuman erythrocytes. This work highlights the potential forrational design and synthesis of improved antimicrobialpeptides that have the capability to be used therapeuticallyfor treatment of cancers.

Study on the mechanical behaviors of timber frame with the simplified column foot joints

Qing-shan Yang,Jun-xiao He,Juan Wang 국제구조공학회 2021 Structural Engineering and Mechanics, An Int'l Jou Vol.77 No.3

Column foot in traditional Chinese timber structures may be subjected to be uplifted due to the lateral load and subsequently reset under the vertical loads. The residual moment of the rocking column foot is the most important parameter representing the mechanical behaviors of column foot, and the simplification of joints is the basis of structural analysis of whole structure. The complicated mechanical behaviors of joint and the modeling of the column foot joint has been undertaken historically based on the experiments and numerical simulation. On the condition of limited application range of those models, a lack of simplified model to represent the mechanical behaviors of joint deserves attentions. There is a great need to undertake theoretical studies to derive the residual moment and make better simplified model of the joint. This paper proposes the residual moment and equivalent simplified model of the rotational stiffness for column foot joint. And, the timber frame is established based on the simplified model, which is verified by solid finite element model. Results show that a mutual agreement on the mechanical behaviors of the timber frame is obtained between the simplified model and the solid finite element model. This study can serve as the references of the structural analysis for the traditional timber structures.

LSTM Android Malicious Behavior Analysis Based on Feature Weighting

( Qing Yang ),( Xiaoliang Wang ),( Jing Zheng ),( Wenqi Ge ),( Ming Bai ),( Frank Jiang ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.6

With the rapid development of mobile Internet, smart phones have been widely popularized, among which Android platform dominates. Due to it is open source, malware on the Android platform is rampant. In order to improve the efficiency of malware detection, this paper proposes deep learning Android malicious detection system based on behavior features. First of all, the detection system adopts the static analysis method to extract different types of behavior features from Android applications, and extract sensitive behavior features through Term frequency-inverse Document Frequency algorithm for each extracted behavior feature to construct detection features through unified abstract expression. Secondly, Long Short-Term Memory neural network model is established to select and learn from the extracted attributes and the learned attributes are used to detect Android malicious applications, Analysis and further optimization of the application behavior parameters, so as to build a deep learning Android malicious detection method based on feature analysis. We use different types of features to evaluate our method and compare it with various machine learning-based methods. Study shows that it outperforms most existing machine learning based approaches and detects 95.31% of the malware.

Diabetes Mellitus Increases the Risk of Bladder Cancer: An Updated Meta-analysis

Yang, Xiao-Qing,Xu, Chen,Sun, Yan,Han, Rui-Fa Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4

Purpose: Studies have indicated that diabetes mellitus (DM) is a risk factor for bladder cancer; however, not all evidence supports this conclusion. The aim of this meta-analysis was to collate and evaluate all primary observational studies investigating the risk of bladder cancer associated with DM. Methods: The PubMed and Google Scholar databases were searched to identify studies that estimated the association of DM and bladder cancer. Summary effect estimates were derived using a random-effects meta-analysis model. Results: A total of 23 studies (8 case-control studies, 15 cohort studies) including 643,683 DM and 4,819,656 non-DM cases were identified. Analysis of all studies showed that DM was associated with an increased risk of bladder cancer compared with non-DM overall (OR=1.68, 95% CI 1.32-2.13). Analysis of subgroups demonstrated this to be the case in both case-control studies (OR=1.59, 95% CI 1.28-1.97, $I^2$=58%) and cohort studies (RR=1.70, 95% CI 1.23-2.33, $I^2$=96%). There was no gender difference in DM-associated bladder cancer risk. Bladder cancer risk was increased in Asia and the North America region, but not in Europe. Furthermore, DM-associated bladder cancer risk was obviously higher in Asia than North America and Europe or in those with Caucasian ethnicity. With extension of follow-up time, the bladder cancer risk was not increased for the patients with DM. Conclusions: This meta-analysis provided further evidence supporting theDM association with a significantly higher risk of bladder cancer obtained from observational studies.

Effect of Channel Curvature for Jet Impinging on Blade Leading Edge

Qing-Yang Zhao,Eui Yeop Jung,Seok Min Choi,Hyung Hee Cho 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12

The characteristics of fluid flow and heat transfer affected by the curvature variation in a jet impinged leading edge channel have been investigated numerically using three-dimensional Reynolds-averaged Navier-Stokes analysis via shear stress transport turbulence model and γ - Re<SUB>θ</SUB> transitional turbulence model. A constant heat flux condition has been applied on the leading edge surface. Jet to leading edge surface distance is constant as three times of jet diameter. The leading edge curved surface curvature is set as variables by the reason of multiple shape of real turbine blade. Results presented in this study include contour of Nusselt number, velocity vector and local Nusselt number distribution along the central line on the leading edge surface. Based on the data above, the average Nusselt number, stagnation Nusselt number along the central line of leading edge and average pressure supplied on each jet are compared between each case. The varied leading edge curved surfaces change the cross flow intensity which influences the impinging jet flow direction. As a result of the phenomenon of jet flow direction change, the heat transfer on the leading edge is influenced significantly. Result indicates that with the decrease of leading edge curved surface diameter, the cross flow effect improves and heat transfer uniformity decreases. In the case of small diameter leading edge, the average heat transfer decrease and the Nusselt value decreases along the streamwise direction due to the crossflow from upstream jet flow. Besides the heat transfer uniformity, the thermal performance comparison is also investigated in this study.