Microarray Analysis of Long Non-coding RNA Expression Profile Associated with 5-Fluorouracil-Based Chemoradiation Resistance in Colorectal Cancer Cells

Xiong, Wei,Jiang, Yong-Xin,Ai, Yi-Qin,Liu, Shan,Wu, Xing-Rao,Cui, Jian-Guo,Qin, Ji-Yong,Liu, Yan,Xia, Yao-Xiong,Ju, Yun-He,He, Wen-Jie,Wang, Yong,Li, Yun-Fen,Hou, Yu,Wang, Li,Li, Wen-Hui Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

Background: Preoperative 5-fluorouracil (5-FU)-based chemoradiotherapy is a standard treatment for locally advanced colorectal cancer (CRC). However, CRC cells often develop chemoradiation resistance (CRR). Recent studies have shown that long non-coding RNA (lncRNA) plays critical roles in a myriad of biological processes and human diseases, as well as chemotherapy resistance. Since the roles of lncRNAs in 5-FU-based CRR in human CRC cells remain unknown, they were investigated in this study. Materials and Methods: A 5-FU-based concurrent CRR cell model was established using human CRC cell line HCT116. Microarray expression profiling of lncRNAs and mRNAs was undertaken in parental HCT116 and 5-FU-based CRR cell lines. Results: In total, 2,662 differentially expressed lncRNAs and 2,398 mRNAs were identified in 5-FU-based CRR HCT116 cells when compared with those in parental HCT116. Moreover, 6 lncRNAs and 6 mRNAs found to be differentially expressed were validated by quantitative real time PCR (qRT-PCR). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis for the differentially expressed mRNAs indicated involvement of many, such as Jak-STAT, PI3K-Akt and NF-kappa B signaling pathways. To better understand the molecular basis of 5-FU-based CRR in CRC cells, correlated expression networks were constructed based on 8 intergenic lncRNAs and their nearby coding genes. Conclusions: Changes in lncRNA expression are involved in 5-FU-based CRR in CRC cells. These findings may provide novel insight for the prognosis and prediction of response to therapy in CRC patients.

Roles of Immunohistochemical Staining in Diagnosing Pulmonary Squamous Cell Carcinoma

Yan, Yue,Zhang, Ya-Xiong,Fang, Wen-Feng,Kang, Shi-Yang,Zhan, Jian-Hua,Chen, Nan,Hong, Shao-Dong,Liang, Wen-Hua,Tang, Yan-Na,He, Da-Cheng,Wu, Xuan,Zhang, Li Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

Background: Differentiating morphologic features based on hematoxylin-eosin (HE) staining is the most common method to classify pathological subtypes of non-small-cell lung cancer (NSCLC). However, its accuracy and inter-observer reproducibility in pathological diagnosis of poorly differentiated NSCLC remained to be improved. Materials and Methods: We attempted to explore the role of immunohistochemistry (IHC) staining in diagnosing pulmonary squamous cell carcinoma (SQCC) with poorly differentiated features by HE staining or with elevated serum adenocarcinoma-specific tumor markers (AD-TMs). We also compared the difference of epidermal growth factor receptor (EGFR) mutation rate between patients with confirmed SQCC and those with revised pathological subtype. Logistic regression analyses were used to test the association between different factors and diagnostic accuracy. Results: A total of 132 patients who met the eligible criteria and had adequate specimens for IHC confirmation were included. Pathological revised cases in poor differentiated subgroup, biopsy samples and high-level AD-TMs cases were more than those with high/moderate differentiation, surgical specimens and normal-level AD-TMs. Moreover, biopsy sample was a significant factor decreasing diagnostic accuracy of pathological subtype (OR, 4.037; 95% CI 1.446-11.267, p=0.008). Additionally, EGFR mutation rate was higher in patients with pathological diagnostic changes than those with confirmed SQCC (16.7% vs 4.4%, p=0.157). Conclusions: Diagnosis based on HE staining only might cause pathological misinterpretation in NSCLC patients with poor differentiation or high-level AD-TMs, especially those with biopsy samples. HE staining and IHC should be combined as pathological diagnostic standard. The occurrence of EGFR mutations in pulmonary SQCC might be overestimated.

Knockdown of endogenous SKIP gene enhance insulin-induced glycogen synthesis signaling differentiating C2C12 myoblasts

( Qi Xiong ),( Chang Yan Deng ),( Jin Chai ),( Si Wen Jiang ),( Yuan Zhu Xiong ),( Feng E Li ),( Rong Zheng ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.2

국가이미지, 제품이미지 및 구매의도의 관계

웅건용(Xiong Jian-yong),무연연(Wu Yan-yan),김원겸(Kim Won-kyum) 한국유통학회 2010 한국유통학회 학술대회 발표논문집 Vol.2010 No.8

본 연구는 한국에서 중국산 의류제품을 대상으로 중국 국가이미, 제품이미지 및 구매의도의 관계를 고찰하는데 주된 목적을 두었다. 분석 결과 국가이이미 중 경제적 이미지, 문화적 이미지가 제품이미지에 긍정적인 영향을 미치는 것으로 나타났고, 제품이미지가 고객 구애의도에 긍정적인 영향을 미치는 것으로 나타났다. 연구 결과를 종합하면 국가이미지를 중심으로 제품의 이미지에 초점을 맞추어 촉진활동을 하면 소비자의 구매행동유도에 효과적인 결과를 얻을 수 있을 것이다. 따라서 중국산 의류제품의 발전을 도모하기 위해서는 국가이미지가 제품이미지 및 구매의도를 향상시킬 수 있음을 시사하고 있다. This study is about to reveal the connections and the importance of China's country image, product image, and purchase intention Following are the results of the empirical analysis summary. First, in country's national image, except political image, economic image and cultural image have a positive (+) impact on the product image is true. Secondly, a country's product image have a positive (+) impact on purchase intention. Therefore, people tend to improve purchase intention when China's product image increase. In summary, consumers' purchase intention can obtain the best positive result, when a country's national image is set as the core factor, which combines and improves concurrently with one's product image.

Transport of macromolecules through plasmodesmata and the phloem

Chen, Xiong-Yan,Kim, Jae-Yean Blackwell Publishing Ltd 2006 Physiologia plantarum Vol.126 No.4

<P>Cell-to-cell communication is a pivotal process in the determination of cell fate during development and physiological adaptation in response to environmental stimuli. The intercellular trafficking of proteins and RNAs has emerged as a novel mechanism of cell-to-cell signaling in plants. As a strategy for efficient intercellular communication, plants have evolved plant-specific symplasmic communication networks via plasmodesmata (PD) and the phloem. PD are symplasmic channels connecting the cytoplasm of neighboring cells and are responsible for the local exchange of metabolites and signaling molecules. The phloem is the sieve-tube system that allows rapid, long-distance translocation of molecules. Together, PD and phloem conduits have been shown to allow the transport of proteins and RNAs in non-selective or/and selective modes. This review describes the current understanding of macromolecule trafficking through PD and the phloem.</P>

The Arabidopsis callose synthase gene GSL8 is required for cytokinesis and cell patterning.

Chen, Xiong-Yan,Liu, Lin,Lee, Eunkyoung,Han, Xiao,Rim, Yeonggil,Chu, Hyosub,Kim, Seon-Won,Sack, Fred,Kim, Jae-Yean American Society of Plant Physiologists 2009 Plant Physiology Vol.150 No.1

<P>Cytokinesis is the division of the cytoplasm and its separation into two daughter cells. Cell plate growth and cytokinesis appear to require callose, but direct functional evidence is still lacking. To determine the role of callose and its synthesis during cytokinesis, we identified and characterized mutants in many members of the GLUCAN SYNTHASE-LIKE (GSL; or CALLOSE SYNTHASE) gene family in Arabidopsis (Arabidopsis thaliana). Most gsl mutants (gsl1-gsl7, gsl9, gsl11, and gsl12) exhibited roughly normal seedling growth and development. However, mutations in GSL8, which were previously reported to be gametophytic lethal, were found to produce seedlings with pleiotropic defects during embryogenesis and early vegetative growth. We found cell wall stubs, two nuclei in one cell, and other defects in cell division in homozygous gsl8 insertional alleles. In addition, gsl8 mutants and inducible RNA interference lines of GSL8 showed reduced callose deposition at cell plates and/or new cell walls. Together, these data show that the GSL8 gene encodes a putative callose synthase required for cytokinesis and seedling maturation. In addition, gsl8 mutants disrupt cellular and tissue-level patterning, as shown by the presence of clusters of stomata in direct contact and by islands of excessive cell proliferation in the developing epidermis. Thus, GSL8 is required for patterning as well as cytokinesis during Arabidopsis development.</P>

국가이미지, 제품이미지 및 구매의도의 관계

웅건용(Jian-Yong Xiong),무연연(Yan-Yan Wu),김원겸(Won-Kyum Kim) 한국콘텐츠학회 2010 한국콘텐츠학회논문지 Vol.10 No.12

본 연구는 한국 대학생들을 대상으로 중국산 의류제품에 대한 중국 국가이미, 제품이미지 및 구매의도의 관계를 고찰하는데 주된 목적을 두었다. 분석 결과 중국 국가이미지 중 경제적 이미지, 문화적 이미지가 제품이미지에 긍정적인 영향을 미치고, 제품이미지는 구매의도에 긍정적인 영향을 미치는 것으로 나타났다. 연구 결과에 따르면 국가이미지를 중심으로 제품의 이미지에 초점을 맞추어 촉진활동을 하면 소비자의 구매행동유도에 효과적인 결과를 얻을 수 있을 것이다. 따라서 중국산 의류제품의 발전을 도모하기 위해서는 먼저 중국 국가차원의 국가이미지를 제고해야 하며, 그 결과로 제품이미지 및 구매의도를 향상시킬 수 있음을 시사하고 있다. This study is about to reveal the connections and the importance of China's country image, product image, and purchase intention. Following are the results of the empirical analysis summary. First, in country’s national image, except political image, economic image and cultural image have a positive (+) impact on the product image is true. Secondly, a country’s product image have a positive (+) impact on purchase intention. Therefore, consumer tend to improve purchase intention when China’s product image increase. In summary, consumers’ purchase intention can obtain the best positive result, when a country’s national image is set as the core factor, which combines and improves concurrently with one’s product image.

국가이미지, 제품이미지 및 구매의도의 관계

웅건용(Jian Young Xiong),무연연(Yan Yan Wu),김원겸(Won Kyum Kim) 한국경영학회 2010 한국경영학회 통합학술발표논문집 Vol.2010 No.08

ZNF424, a novel human KRAB/C2H2 zinc finger protein, suppresses NFAT and p21 pathway

( Yue Qun Wang ),( Jun Mei Zhou ),( Xiang Li Ye ),( Yong Qi Wan ),( Yong Qing Li ),( Xiao Yan Mo ),( Wu Zhou Yuan ),( Yan Yan ),( Na Luo ),( Ze Qun Wang ),( Xiong Wei Fan ),( Yun Deng ),( Xiu Shan Wu 한국생화학분자생물학회 (구 한국생화학회) 2010 BMB Reports Vol.43 No.3

Zinc finger-containing transcription factors are the largest single family of transcriptional regulators in mammals, which play an essential role in cell differentiation, cell proliferation, apoptosis, and neoplastic transformation. Here we have cloned a novel KRAB-related zinc finger gene, ZNF424, encoding a protein of 555aa. ZNF424 gene consisted of 4 exons and 3 introns, and mapped to chromosome 19p13.3. ZNF424 gene was ubiquitously expressed in human embryo tissues by Northern blot analysis. ZNF424 is conserved across species in evolution. Using a GFP-labeled ZNF424 protein, we demonstrate that ZNF424 localizes mostly in the nucleus. Transcriptional activity assays shows ZNF424 suppresses transcriptional activity of L8G5-luciferase. Overexpression of ZNF424 in HEK- 293 cells inhibited the transcriptional activity of NFAT and p21, which may be silenced by siRNA. The results suggest that ZNF424 protein may act as a transcriptional repressor that suppresses NFAT and p21 pathway to mediate cellular functions. [BMB reports 2010; 43(3): 212-218]

Expression of Glypican-3 is Highly Associated with Pediatric Hepatoblastoma: a Systemic Analysis

Xiong, Xiao-Li,Qin, Huan,Yan, Su-Qi,Zhou, Li-Shan,Chen, Peng,Zhao, Dong-Chi Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.3

Objective: Glypican-3 (GPC3) is reported to be an oncofetal protein that is a useful diagnostic immunomarker for hepatoblastoma. However, the results are not inclusive. This study systemically investigated the association between expression of GPC3 and pediatric hepatoblastoma. Methods: Clinical studies evaluating the association were identified using a predefined search strategy. GPC3 immunohistochemistry was applied in the pathological diagnosis of hepatoblastoma using the monoclonal antibodies with formalin-fixed and paraffin-embedded specimens. Positive predictive rates for the association between expression of GPC3 and pediatric hepatoblastoma were calculated. Results: Specimens from four clinical studies which including 134 patients with pediatric hepatoblastoma tested by GPC3 immunohistochemistry were considered eligible for inclusion. Systemic analysis showed that, in all patients, pooled positive predictive rate of the association between expression of GPC3 and pediatric hepatoblastoma was 95.5% (128/134). Conclusion: This systemic analysis suggests that the expression of glypican-3 is highly associated with the diagnosis of pediatric hepatoblastoma.