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진해만산 진주담치 , Mytilus edulis 및 와편모조 , Alexandrium tamarense 의 마비성패독
이종수,전중균,한명수,--,-- 한국수산학회 1992 한국수산과학회지 Vol.25 No.2
1989년 및 1990년도 진해만산 진주담치(Mytilus edulis)와 1989년도산 유독와편모조(Alexandrium tamarense) 배양 조체의 마비성패독 성분의 조성과 독성을 mouse assay법과 post column을 이용한 형광고속액체 chromatography법으로 조사하였다. mouse assay법으로 조사한 진주담치의 독성은 1989년산(3∼4월)이 중장선 1g당 31∼88 MU, 1990년도산이 가식부 1g당 1.9∼9.9 MU이었다. 독조성은 1989년도산의 경우, gonyautoxin 1-4(48∼76%), Cl∼C2(14∼39%), saxitoxin(1∼10%), neosaxitoxin(1∼7%) 그리고 미량의 decarbamoyl gonyautoxin 2,3가 포함되어 있었으며, 1990년도의 경우는 neosaxitoxin의 함량이 44∼50%의 비율을 나타내어 년도에 따라 큰 독조성의 차이를 나타내었다. 한편, A. tamarense의 배양조체의 독조성은 1989년도산 진주담치와 유사하였으나, Cl, C2, decarbamoyl gonyautoxin 2,3의 비율이 다소 높았다. Paralytic shellfish toxins in mussels Mytilus edulis and dinoflagellate Alexandrium tamarense from Jinhae Bay, south coast of Korea were investigated. The mussels collected in March-April, 1989 showed toxicities of 7.5 MU/g of whole meat(31∼88 MU/g of the digestive gland), and those collected in 1990 showed toxicity level of 1.9∼9.9 MU/g of whole meat by the standard mouse bioassay. Analysis of toxins by high performance liquid chromatography revealed the presence of gonyautoxin 1∼4(48∼76%), gonyautoxin 8 and epi-gonyautoxin 8(C1∼C2, 14∼39%), saxitoxin(1∼10%), neosaxitoxin(1∼7%) and trace amount of decarbamoylgonyautoxin 2 and 3(dcGTX2, dcGTX3) in the mussels of 1989. While, Mussels collected in 1990 contained a significantly larger proportion of neosaxitoxin (44∼50% ) than did those of 1989. A. tamarense isolated in April 1989 produced the same toxins in culture with slightly higher proportion of C1, C2, dcGTX2 and dcGTX3 than in the mussels. The difference was within a range of toxin change during accumulation by shellfish and during sample preparation for analysis. It was thus concluded that the dinoflagellate was the cause of toxins in the mussels.
( Jong Soo Lee ),( Masayuki Satake ),( Yoichi Horigome ),( Yasukatsu Oshima ),( Takeshi Yasumoto ) 한국수산과학회(구 한국수산학회) 2012 Fisheries and Aquatic Sciences Vol.15 No.1
A new cyclic peptide (six-membered amino acid), gamakamide-E (L-Leu-L-Met (SO)-L-Me-Phe-L-Leu-D-Lys-L-Phe), was isolated as a strongly bitter tasting compound from cultured oysters, Crassostrea gigas. The molecular formula of C43H61N7O8S was deduced from high resolution fast atom bom bardment mass spectrometry (HR FAB-MS) ([M + H]+ m/z 836.4356 △= -2.4 mmu). Its unique structure including a hydantoin structure was firstly elucidated by nuclear magnetic resonance (NMR) analysis. Stereochemistries of constituent amino acids were determined by chiral high performanced liquid chromatography analysis of natural and synthesized peptides.
Lee, Jong-Soo,Satake, Masayuki,Horigome, Yoichi,Oshima, Yasukatsu,Yasumoto, Takeshi The Korean Society of Fisheries and Aquatic Scienc 2012 Fisheries and Aquatic Sciences Vol.15 No.1
A new cyclic peptide (six-membered amino acid), gamakamide-E (L-Leu-L-Met (SO)-L-Me-Phe-L-Leu-D-Lys-L-Phe), was isolated as a strongly bitter tasting compound from cultured oysters, Crassostrea gigas. The molecular formula of $C_{43}H_{61}N_7O_8S$ was deduced from high resolution fast atom bombardment mass spectrometry (HR FAB-MS) ($[M+H]^+$ m/z 836.4356 ${\Delta}$= -2.4 mmu). Its unique structure including a hydantoin structure was firstly elucidated by nuclear magnetic resonance (NMR) analysis. Stereochemistries of constituent amino acids were determined by chiral high performanced liquid chromatography analysis of natural and synthesized peptides.
Determination of diarrhetic shellfish toxins in various dinoflagellate species
Lee, Jong-Soo,Igarashi, Teruo,Fraga, Santiago,Dahl, Einal,Hovgaard, Peter,Yasumoto, Takeshi 國立統營水産專門大學 附設 水産科學硏究所 1989 수산과학연구소보고 Vol.1 No.-
Sixteen species of unialgal samples of dinoflagellate, either wild or cultured, were tested for production of diarrhetic shellfish toxins such as okadaic acid (OA), dinophysistoxin-1 (DTX1), and pectenotoxins(PTXs). Determination of micro-quantities of the toxins was facilitated by fluorometry and UV HPLC. Seven Dinophysis species were confirmed to produce either OA or DTX1, or both. Toxin content and composition varied regionally and seasonally. Intraspecies variation was also observed among cultured strains of prorocentrum lima. PTX2 was the only toxin detected among PTX family, and D. fortii was the only species to contain this toxin.