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Beak, Sung Mok,Lee, Yong Soo,Kim, Jung-Ae 德成女子大學校 藥學硏究所 2004 藥學論文誌 Vol.15 No.1
The detrimental effects of ultraviolet B (UVB) inaddition have been connected with the enhanced generation of reactive oxygen species (ROS) by UVB. However, the exact source of ROS produced by UVB has not been clearly revealed yet. In this study, we determined the source of ROS production and its tole in the UVB-induced activation of nuclear factor (NF)-kB in HaCaT human keratinocytes. UVB irradiation generated ROS in a dose-dependent manner. and this was significantly inhibited by diphenylene it lonium (DPI), apocynin (Apo) and neopterine (Neo). inhibitors of the NADPH oxidase, and indomethaein (Indo), a cvelooxy genase (COX) inhibitor. hut not by the mitochondria) electron transport inhibitors and other cytosolie enzyme inhibitors. In addition, these inhibitors of theNADPH oxidase and COX significantly blocked the UVB irradiation-induced nuclear translocation of NF-kB. These results suggest that the NADPH oxidase and COX may be major sources for the UVB-induced ROS generation, and play an essential role in the activation of NF-kB which is involved in the expression of a variety of genes induced by UVB in HaCaT cells. These results further suggest that these enzymes may be good targets for the preventive strategy of UVB-induced skin injury.
Beak, Sung-Mok,Paek, Seung-Hwan,Jahng, Yurngdong,Lee, Yong Soo,Kim, Jung-Ae 德成女子大學校 藥學硏究所 2004 藥學論文誌 Vol.15 No.1
Matrix metalloproteinases (MMPs), a key component in photoaging of the skin due to exposure to ultraviolet A, appear to he increased by ultraviolet A irradiation-associated generation of reactive oxygen species. In this study, we investigated the effects of synthetic rutaecarpine. which is also found in Evocdia rutaercztpa. on the ultraviolet A-induced changes in the expression of gelatinases: matrix metalloproteinase (MMP)-2 and MMP-9 using HaCaT human keratinocytes as a model cellular system Ultraviolet A irradiation of HaCaT cells increased the gelatinolytic activities of MMP-2 and MMP-9, which was significantly suppressed by the pretreatment with rutaecarpine. In addition, rutaecarpine significantly suppressed the ultraviolet A-induced enhanced expression of MMP-2 and MMP-9 proteins and mRNAs. Rutaecarpine also inhibited the H₂O₂-induced increase in the expression of MMP-2 and MMP-9. Furthenuore, rutaecarpine decreased the ultraviolet A-induced increased generation of reactive oxygen species. taken together, these results suggest that rutaccarpine inhibited ultraviolet A-induced reactive oxygen species generation. resulting in the enhanced expression of MMP-2 and MMP-9 in human skin cells. These results further suggest that ruetaccaipine may be useful in the prevention of ultraviolet A-induced photoaging.
이상철(Sang Chull Lee),김여갑(Yeo Gab Kim),류동목(Dong Mok Ryu),이백수(Beak Soo Lee),오승환(Sung Whan Oh),윤옥병(Ok Byung Yoon),신민철(Min Cheol Shin) 대한구강악안면외과학회 1996 대한구강악안면외과학회지 Vol.22 No.3
Osteochondroma is the most common benign tumor of axial and appendicular skeletons. While this tumor occurs at long bones exclusively, however, the lesion have been reported in many other skeletal site including the scapula, vertebra, ilium and mandible. In the mandible, most of lesions involve the coronoid process and the condylar osteochondroma is very rare. Condylar osteochondroma may manifests varying clinical signs and symptoms which include prognathic deviation of the mandible to the opposite side, malocclusion, cervicofacial pain and limited mouth opening. The majority of surgical treatments have been condylectomy. But recently, simple extirpation of lesion with condylar salvage were reported to maintain functions of mandible and the ramal height postoperatively. We have experienced two cases of condylar osteochondroma treated with simple extirpation of lesion and condylar shaving, and would like to report these with review of literatures.
LEE, Jae Sung,LEE, Jong Suk,YOON, Jae-Don,BEAK, Sung-Mok,BOSIRE, Kefa O.,LEE, Yong-Soo,KIM, Jung-Ae 德成女子大學校 藥學硏究所 2004 藥學論文誌 Vol.15 No.1
Over the past several years, research efforts have been directed both at economically producing valuable substances from the wood biomass and at producing lignolytic enzymes at a lower cost. In the present study, we found that Phellinus igniaruas, the basidiomycetes, secreted lignin peroxidase as a main lignolytic enzyme, which was detected maximum activity at 16th day of culture and showed 37 kDa of molecular mass in identification by activity assay and purification by anion-exchange chromatography. The Phellinus igniarius-derived lignin peroxidase hydrolyzed steam-exploded wood (Quercus ntongolica) powder into small molecules showing cytotoxicity against cancer cells (HepG2 hepatoma, SK-N-SH neuroblastoma, B16 melanoma, MBT-2 bladder cancer). ha addition, the enzyme hydrolysates of lignins (ELg) that were extracted from the steam-exploded oak showed more potent cytotoxic effects on the cancer cells than the enzyme hydrolysates of wood biomass (EWp). indicating that the cytotoxic effect of EWp may be due to the enzyme-degraded products of lignin among the lignocellulosics. Furthermore, the cytotoxic effect of ELg on Chang, normal liver cells, was much less potent than that of ELg on HepG2 and B16 cancer cells, indicating that the cytotoxic effect of ELg may be specific for cancer cells. The present results suggest that Phellinus igniarius may be a useful resource for the large-scale production of lignin peroxidase and that the lignin peroxidase may be applied for the generation of valuable biodegradation products from wood lignocellulosics for medical use.
Lee, Yong Soo,Jin, Da-Qing,Beak, Sung-Mok,Lee, Eung-Seok,Kim, Jung-Ae 德成女子大學校 藥學硏究所 2003 藥學論文誌 Vol.14 No.1
Premature aging of the skin (photoaging) is a well-documented consequence of exposure to ultraviolet-A (UVA). Enhanced generation of reactive oxygen species and induction of matrix metalloproteinases (MMPs) appear to be the most important components of UVA-modulated signal transduction pathways, ultimately leading to photoaging. In this study, we investigated the effects of asiatic acid and ursolic acid, triterpene compounds, on the UVA-modulated signaling pathways, using HaCaT human keratinocytes as a model cellular system. In the cells, we confirmed that UVA irradiation induced oxidative stress and increased the expression of MMP-2. Asiatic acid and ursolic acid significantly reduced the UVA-induced activation and expression of MMP-2. In addition, UVA-induced enhanced expression of p53, a hallmark of UV-induced DNA damage and cell death, was also significantly inhibited by pretreatment with asiatic acid or ursolic acid. Taken together, these results suggest that asiatic acid and ursolic acid may be an effective inhibitor of UVA-modulated signal transduction patyways in human skin cells. These results further suggest that these agents may be useful in the prevention of UVA-induced photoaging.