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Assessment of In Vitro Assay System for Thyroid HormoneDisruptors Using Rat Pituitary GH3 Cells
Hee Jin Kim1,Hae Young Park1,Jeonga Kim1,Il Hyun Kang2,Tae Sung Kim2,Soon Young Han2,Tae Seok Kang2,Kui Lea Park2,Hyung Sik Kim1 한국독성학회 2006 Toxicological Research Vol.22 No.4
The development of in vitro assays has been recommended to screening and test-ing the potential endocrine disruptors (EDs). These assay systems focus only on identifying thethe thyroid hormone (TH) disruptors. The aim of this study was to evaluate a test system to detectTH disruptors using rat pituitary tumor GH3 cells. The test system is based on the TH-dependentincrease in growth rate. As expected, L-3,5,3-triiodothyronine (T3) markedly induced a morphologicalchange in GH 3 cells from flattened fibroblastic types to rounded or spindle-shaped types. T3 stimu-lated GH3 cell growth in a dose-dependent manner with the maximum growth-stimulating effect9 M. In addition, T3 increased the release of growth hor-mone and prolactin into the medium of the GH3 cells culture. Using this assay system, the TH-dis-rupting activities of bisphenol A (BPA) and its related compounds were examined. BPA,dimethylbisphenol A (DMBPA), and TCI-EP significantly enhanced the growth of GH3 cells in therange of 1 × 10-5M to 1 × 10-6M concentrations. In conclusion, this in vitro assay system might bestandardization before it can be used as a broad-based screening tool.
종 분포 모형을 이용한 구상나무림의 지속 및 쇠퇴에 관한 연구 - 전라남도 광양시 백운산을 중심으로 -
조선희 ( Seon Hee Cho ),박종영 ( Jong Young Park ),박정호 ( Jeong Ho Park ),이양근 ( Yang Geun Lee ),문이만 ( Lee Man Mun ),강상호 ( Sang Ho Kang ),김광현 ( Gwang Hyun Kim ),윤종국 ( Jong Guk Yun ) 한국산림과학회 2015 한국산림과학회지 Vol.104 No.3
The present study investigated the habitats of Korean fir trees (Abies koreana E. H. Wilson) on Mt. Baekwun (Baekwun-san), determined the current distribution, quantified the contribution of biological and nonbiological environmental factors affecting the distribution, derived actual and potential habitats, presented a plan for the establishment of protected areas, applied RCP 8.5 climate change scenario to analyze the effects of climate change on the future distribution of Korean fir trees, and predicted future potential habitats. According to the results of the study, 3,325 Korean fir trees (DBH >= 2.5 cm) inhabited Mt. Baekwun, and their distribution area was approximately 150 ha. Populations of Korean fir trees were confirmed to exist at an altitude of 900 m above sea level and were distributed up to 1,200 m. Based on potential distribution, areas appropriate for habitation by Korean fir trees were analyzed to be 450 ha, three times the current distribution area, with a focus on Sang Peak (Sang-bong), Eokbul Peak (Eokbul-bong), Ddari Peak (Ddari-bong), and Dosol Peak (Dosol-bong). The forest stands near Sang Peak, the main peak, were evaluated as those with the most appropriate potential for the habitation of Korean fir trees, and populations of the trees tended to prefer the northern slope rather than the southern slope. When climate change scenario RCP 8.5 was applied and future potential distribution was analyzed, the habitats were expected to decrease in area to 20 ha by 2050, with a focus on Sang Peak, and areas appropriate for habitation were predicted not to exist by 2080. Judging from such results, as global warming accelerates, the habitats of Korean fir trees are clearly expected to move from lowlands to highlands.
Cho, Eun Sook,Jin, Byung Rae,Sohn, Hung Dae,Choi, Kwang Ho,Kim, Soung Ryul,Kang, Seok Woo,Yun, Eun Young,Kim, Sang Hyun,Kim, Keun Young,Je, Yeon Ho,Kang, Seok Kwon 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2
To construct transfurmed Bm5 cells, Autographa californica nuclear polyhedrosis virus(AcNPV) IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% nucleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/ml G4l8 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistant cells was isolated and characterized by PCR using AcNPV IEI gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. mori-derived Bm5 cells as well as Spodoptera fugjprrda-derived Sf9 cells to produce stably-transformed insect cells
<i>Drosophila</i> GPCR Han Is a Receptor for the Circadian Clock Neuropeptide PDF
Hyun, Seogang,Lee, Youngseok,Hong, Sung-Tae,Bang, Sunhoe,Paik, Donggi,Kang, Jongkyun,Shin, Jinwhan,Lee, Jaejung,Jeon, Keunhye,Hwang, Seungyoon,Bae, Eunkyung,Kim, Jaeseob Elsevier 2005 Neuron Vol.48 No.2
<P><B>Summary</B></P><P>The pigment-dispersing factor (PDF) is a neuropeptide controlling circadian behavioral rhythms in <I>Drosophila</I>, but its receptor is not yet known. From a large-scale temperature preference behavior screen in <I>Drosophila</I>, we isolated a P insertion mutant that preferred different temperatures during the day and night. This mutation, which we named <I>han</I>, reduced the transcript level of <I>CG13758</I>. We found that Han was expressed specifically in 13 pairs of circadian clock neurons in the adult brain. <I>han</I> null flies showed arrhythmic circadian behavior in constant darkness. The behavioral characteristics of <I>han</I> null mutants were similar to those of <I>pdf</I> null mutants. We also found that PDF binds specifically to S2 cells expressing Han, which results in the elevation of cAMP synthesis. Therefore, we herein propose that Han is a PDF receptor regulating circadian behavioral rhythm through coordination of activities of clock neurons.</P>
Kang, Han-Ju,Kim, Kyung-Ho,Jo, Hyun-Seog,Hwang, Min-Seob,Kim, Kap-Sung The Korean Acupuncture Moxibustion Medicine Societ 2005 대한침구의학회지 Vol.24 No.5
Objective : The effect of Ulmus davidiana Planch(UD), which has long been known to have anti-inflammation and protective effects on damaged tissue, inflammation and bone among other functions, on the development of type II collagen (CII)-induced arthritis (CIA) in rats was studied. Methods : Male rats were immunized with an emulsion of $200\;{\mu}g$ of CII and complete Freund's adjuvant (CFA). The rats were then given intraperitoneal stimulation of Ulmus davidiana Planch herbal acupuncture(UDHA)or saline during the experiment. When compared with rats treated with saline as control, UDHA at doses of more than $20{\mu}g/100\;g$ rat once a day for 7 days inhibited the ability of inguinal lymph node cells to produce T cell cytokines interleukin-2, interleukin-6, $IFN-{\gamma}$ when the cells were obtained from rats 14 days after immunization and cultured in vitro with CII. Results : When rats were injected intraperitoneally, UD -treated group and control group rats did not differ significantly when low doses of UD was given to rats. Conclusion : The recommended dose of UD in the management and treatment of rat CIA will be more than $20{\mu}g/100\;g$, which is two-firth of human therapeutic dose. From the results, it was concluded that the effect of UDHA is dependent of dosage.
Modelization of Lentinula edodes Incubation Processing
Hyun You Chang,Geum Hui Seo,Yong Kuk Lee,Dong Yun Kang,Ji Hyun Jung,Sung Woo Jeon 한국버섯학회 2017 버섯 Vol.21 No.2
This study was carried out to investigate the management characteristics and techniques by comparing and analyzing the process of mixing, sterilization, cooling, inoculation and incubation for bag cultivation of L. edodes. The medium was sterilized after mixing it for 30 minutes, 1 and 2 hours respectively. The general moisture content of the sawdust substrate has been adjusted to 65%, while the case of the L. edodes substrate was 55%. Only 5% of wood chips of 5-8 mm in particle size were mixed to secure the space of the sawdust particles. As a result, mycelial growth was 1.4cm faster and the density was better(+++) than control(++). Wood chips are soaked in advance for a week during winter and 4 days during summer. There is an average number of 1.6x108 (cfu) of microorganisms in the sawdust that has been piled for six months outdoor. In summer, it has to be used immediately after mixing sawdust. High-pressure sterilization should be performed to use as a mushroom spawn, and to improve physical properties, it was great to sterilize the medium at a normal-pressure. There are height and width type for bags to be consumed for bagging. When the height was reduced into 17cm and the width was increased into 13cm, the browning period was shortened by 30 days and the period of mycelial growth was shortened by 25 days. The sterilized medium had an average of 4x103(cfu) of microorganisms at 25°C and 1.3×102 (cfu) at 15°C. After 25 days from inoculation in vitro, the growth condition of sawdust was the best with 13.2 cm, followed by grain spawn and liquid respectively. When inoculated with liquid spawn, the moisture content of substrate should be adjusted to 55% to 50% in advance.
( Hyun Ae Jung ),( Young Eun Ha ),( Da Min Kim ),( Ji Hyun Park ),( Cheol In Kang ),( Doo Ryeon Chung ),( Seung Woo Park ),( Ki Ik Sung ),( Jae Hoon Song ),( Kyong Ran Peck ) 대한내과학회 2014 The Korean Journal of Internal Medicine Vol.29 No.3
Klebsiella species are common pathogensthat cause community-onsetand hospital-acquired pneumonia,intra-abdominal infections, andbloodstream infections. Infective endocarditisdue to Klebsiella species israre, accounting for less than 1% ofcases, and is frequently accompaniedby complications and a high in-hospitalmortality rate [1]. Moreover, withthe emergence of extended-spectrumb-lactamase (ESBL)-producing Enterobacteriaceae,endocarditis due toESBL-producing Klebsiella pneumonia(ESBL-KP) is of concern due to thelimited treatment options and notoriouslyhigh morbidity and mortality. Hospitalization is a significant riskfactor for bloodstream infections dueto ESBL-producing Enterobacteriaceae,but no cases of health-care-associatednative valve endocarditis causedby ESBL-KP have been reported. Here,we report a case of health-care-associatedinfective endocarditis due toESBL-KP with multiple metastaticinfectious complications, and discussthe optimal treatment strategies.
CDX1 and CDX2 Expression in Intestinal Metaplasia, Dysplasia and Gastric Cancer
Kang, Jung Mook,Lee, Byoung Hwan,Kim, Nayoung,Lee, Hye Seung,Lee, Hee Eun,Park, Ji Hyun,Kim, Joo Sung,Jung, Hyun Chae,Song, In Sung The Korean Academy of Medical Sciences 2011 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.26 No.5
<P>Intestinal metaplasia (IM) has been regarded as a premalignant condition. However, the pathogenesis of IM is not fully understood. The aim of this study was to evaluate the role of CDX1 and CDX2 in the formation of IM and the progression to dysplasia and gastric cancer (GC). A total of 270 subjects included 90 with GC, dysplasia and age- and sex-matched controls. Real-time PCR (RT-PCR) was performed with body specimens for CDX1 and CDX2. The expression of CDX2 was significantly higher in <I>H. pylori</I> positive group than <I>H. pylori</I> negative group (<I>P</I> = 0.045). CDX1 and CDX2 expression increased proportional to the IM grade of the body (<I>P</I> < 0.001). CDX2 expression was significantly higher in incomplete type of IM than in complete type (<I>P</I> = 0.045). The expression of CDX1 in dysplasia group was significantly higher than in the control group (<I>P</I> = 0.001); in addition, CDX1 and CDX2 in cancer group was significantly higher than control group (<I>P</I> < 0.001, and <I>P</I> < 0.001, respectively). Aberrant expression of CDX1 and CDX2 correlated with <I>H. pylori</I> infection and grade of IM in the body. Furthermore, the results suggest that CDX1 and CDX2 play a role in the progression to GC and dysplasia.</P>
Kang, Hio Chung,Kim, Il-Jin,Park, Jae-Hyun,Shin, Yong,Jang, Sang-Geun,Ahn, Sun-A,Park, Hye-Won,Lim, Sun-Kyung,Oh, Seung Keun,Kim, Dae Jung,Lee, Kwan Woo,Choi, Young-Sik,Park, Young Joo,Lee, Min Ro,Kim National Hellenic Research Foundation 2005 ONCOLOGY REPORTS Vol.14 No.4
<P>Von Hippel-Lindau (VHL) disease is an autosomal dominant inherited tumor syndrome associated with germline mutations in the VHL gene. VHL disease, as well as several other cancer syndromes, has been associated with an increased risk of pheochromocytomas, which are catecholamine-secreting tumors of the adrenal gland. VHL disease genotype-phenotype correlations have been well established based on the type of mutations in the VHL gene. However, although many groups have reported VHL germline mutations in different countries, no previous report has described VHL gene mutations in VHL disease and/or pheochromocytoma patients in Korea. In this study, we used direct sequencing to investigate VHL germline mutations in Korean patients with VHL disease or pheochromocytomas (11 VHL patients and 3 additional members from 7 families, 2 patients from 1 family with familial pheochromocytoma, and 2 cases of isolated pheochromocytoma). We found a total of 7 VHL germline mutations (6 missense and 1 frameshift), 3 of which were novel (323_324delGC, 355T>C and 361G>A). No VHL germline mutation was found in the 2 patients with isolated pheochromocytomas and paragangliomas. This study provides informative data for VHL germline mutations and VHL-related phenotypes in Korea.</P>