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Singh, Ravindra,Jayaswal, K.P.,Rao, D.Raghavedra,Kariappa, B.K.,Premalatha, V. Korean Society of Sericultural Science 2002 International Journal of Industrial Entomology Vol.4 No.2
Induction of non-diapause eggs and manifestation of quantitative characters were studied in different seasons by low temperature incubation of eggs of a low yielding diapause with coloured cocoons silkworm breed, RDI of Bombyx mori. Hundred percent of nandiapause eggs were induced when the egg incubation was carried out at 15$^{\circ}C$ followed by incubation initially at $25^{\circ}C$ and then at $15^{\circ}C$. The diapause-nondia-pause relation was found to be reversible. Analysis of variance study demonstrated significant variation in all the quantitative characters whereas significant variation due to different incubation treatments was observed for larval span, fifth instar larval span, cocoon yield/10,000 larvae by weight, cocoon weight, cocoon shell weight, and cocoon shell ratio. study on hybrid vigour skewed significant heterosis over mid parent value for four economic characters viz., cocoon weight, cocoon shell weight, cocoon shell ratio and filament length in a Fl hybrid between RD1 with diapause eggs and Japanese type bivoltine $NB_{4} D_{2}$.
Singh, Ravindra,Rao, D.Raghavendra,Kariappa, B.K.,Sanyal, Prabhati,Premalatha, V. Korean Society of Sericultural Science 2002 International Journal of Industrial Entomology Vol.5 No.1
Parthenogenetic development, hybrid vigour and cocoon shape variability were studied in bivoltine F1 hybrids of the silkworm, Bombyx mori L. Considerable breed differences were observed in parthenogenetic ability. Among the parental breeds, CSR18 exhibited maximum parthenogenetic development (79.65%) followed by CSR4 (67.90%). Among bivoltine F1 hybrids, CSR18 ${\times}$ CSR4 showed highest parthenogenetic development (73.32%) followed by CSR2 ${\times}$ CSR4 (55.43%). Study on hybrid vigour demonstrated that CSR18 ${\times}$ CSR19 expressed maximum significant hybrid vigour for all the seven economic characters over Mid Parent Value (MPV) followed by CSR2 ${\times}$ CSR4 and CSR18 ${\times}$ CSR4 exhibiting significant heterosis for six characters. Cocoon shape measurement study revealed that among ten bivoltine F1 hybrids, cocoons of three hybrids were found comparatively uniform. Importance of this study to know the level of paythenogenetic development, hybrid vigour and cocoon shape variability in different bivoltine F1 hybrids have been discussed.
Singh, Ravindra,Rao, D.Raghavendra,Premalatha, V.,Mondal, Sipra,Kariappa, B.K.,Jayaswal, K.P.,Datta, R.K. Korean Society of Sericultural Science 2001 International Journal of Industrial Entomology Vol.2 No.2
Data from an experiment involving six polyvoltine and three bivoltine silkworm breeds have been analysed to know the level of hybrid rigour and cocoon shape variation in Fl hybrids among silkworm breeds with lows medium and high cocoon weight. Results indicated significantly positive heterosis for several quantitative characters with maximum total heterosis over mid parent value in hybrids between low and medium followed by low and low, medium and medium, low and high and high and low cocoon-weighed breeds, respectively. Study on cocoon shape measurement revelled that among Fl hybrids, cocoons of the three combinations such as GNP ${\times}$ CSR2, GNP ${\times}$ J2 and PM ${\times}$ NB4 D2 were comparatively uniform in their cocoon shape. Importance of this study to know the level of heterosis and cocoon shape variability in different silkworm crosses hale been discussed.
Ravindra Nath Singh,M. Maheshwari,Beera Saratchandra 한국잠사학회 2004 International Journal of Industrial Entomology Vol.8 No.1
Pest monitoring through field surveys and surveillance helps in forecasting the population build up of pest. It reduces the load of pesticides application and forms the basis of Integrated Pest Management in sericulture. Common sampling techniques for quantifying pest populations and damage caused by them are reviewed emphasizing the need for quick and simple sampling methods. Various direct and indirect sampling methods for establishing pest populations are discussed and methods have been discussed to use indirect sampling method under IPM programme in sericulture. The use of pheromone lures and traps forms one of the important ingredients of integrated pest management, which calls for integration of all available methods in a cost effective and environmental friendly manner offering consistent efficacy. Silkworms feed on the variety of silk host plants and spin cocoons. Each silk host plant is attacked in the field by number of insect pest species. Several pests are common to mulberry, tasar, oak tasar, muga and eri host plant but pest status and seasonal abundance differs from each crop. The key pests are serious perennially occurring persistent species which cause considerable yield loss every year on large areas and require control measure. Regular occurrence of minor pest is noticed but sudden increase in its population is not known. The occasional pests are sporadic but potential causing sufficient damage. Silk losses due to attack of all the pests have not been calculated. However, information on pest biology and ecology, and control practices being practiced is available but the period of outbreak of major pests and predators on silkworms and its host plant needs to be reinvestigated. Pest and predators forecasting based on surveillance information may provide an opportunity to minimize the losses, particularly to reduce expenditure involved in pest management.
Development of a Robust Polyvoltine Breed $'NP_1'$ of the Mulberry Silkworm, Bombyx mori L.
Singh Ravindra,Rao D. Raghavendra,Sharma S.D.,Chandrashekaran K.,Basavaraja H.K.,Kariappa B.K.,Dandin S.B. Korean Society of Sericultural Science 2006 International Journal of Industrial Entomology Vol.12 No.1
A breeding programme was initiated during 2001 utilizing two polyvoltine silkworm breeds viz. $BL_{69}$, an evolved breed tolerant to high temperature and MAR, comparatively resistant to Bombyx mori nuclear polyhedrosis virus (BmNPV) with the objective to develop robust polyvoltine breeds and hybrids. The breed $NP_1$ was developed by exposing the fifth instar larvae to high temperature $(36{\pm}1^{\circ}C)$, high Relative Humidity ($85{\pm}5%$ R.H.) and inoculating third instar larvae with BmNPV inoculum. At $F_{12}$, the breed was tested for hybrid forming ability utilizing six bivoltine silkworm breeds viz. $CSR_2,\;CSR_4,\;CSR_{17},\;CSR_{18},\;CSR_{19}\;and\;NB_4D_2$. The hybrid $'NP_1{\times}CSR_{17}'$ exhibited its superiority by recording 97.2% survival, 1.892 g cocoon weight, 0.406 g cocoon shell weight, 21.5% cocoon shell ratio, 16.6% raw silk percentage and 890 m filament length whereas the control $(PM{\times}CSR_2)$ has recorded 90.2% survival, 1.599 g cocoon weight, 0.304 g cocoon shell weight, 18.9% cocoon shell ratio, 13.1 % raw silk percentage and 768 m filament length. Commercial exploitation of the new $polyvoltine{\times}bivoltine$ hybrid in sericulture industry has been discussed.
Biosensor Arrays for Environmental Pollutants Detection
Ravindra P. Singh,오병근,구기갑,정지영,정시영,최정우 한국바이오칩학회 2008 BioChip Journal Vol.2 No.4
Sensors are an extremely broad field which impacts on many major industrial sectors. This review paper highlights the research carried out during the last 8 years based on recent advances in the development of enzyme and NA (nucleic acid) based biosensors for environmental detection including pollutants and toxic compounds in a wide range of samples. NAbased biosensors are also finding increasing use for the detection of environmental pollutants and their toxicity. Biosensors are now used in a wide variety of disciplines, so that the different types of biosensors are used with their advantages and limitations with different transducers forming the sensing devices, for the different environmental contaminants analysis. The general applications of the enzyme biosensors are highlighted related with an environmental monitoring application. Recent advances in the development and applications of biosensor arrays for environmental detection are highlighted in this review article with special emphasis on functional nucleic acid elements (aptamers, DNAzymes, aptazymes), widely useful in nanobiotechnology and also in lab-on-a-chip technology.
Stimulation of Fecundity through Antennal Amputation in the Mulberry Silkworm, Bombyx mori L.
Singh, Ravindra,Kumar, Virendra,Kariappa, B.K.,Dandin, S.B.,Rao, D.Raghavendra Korean Society of Sericultural Science 2004 International Journal of Industrial Entomology Vol.8 No.2
Stimulation of fecundity following female antennal amputation has been reported for the first time in silkworm. Antennal amputation caused significant increase in fecundity in two newly evolved multivoltine silkworm breeds viz., BL 67 and 96A. This study indicated better chances for increasing egg yield and the increase in fecundity may be attributed to the action of some neurohormones. Significance of antennal amputation in silkworm has been discussed.