In vitro wound healing: Inhibition activity of insect-derived mAb CO17-1A in human colorectal cancer cell migration

Park Se Ra,Ko Kisung,Lim Sohee,Cha So Yeon,정현주,Park Soon Ju,Myung Soon‐Chul,Kim Mi Kyung 한국곤충학회 2020 Entomological Research Vol.50 No.4

The monoclonal antibody (mAb) CO17-1A specifically binds to the tumor associated cell surface glycoprotein GA733 in colorectal cancer cells. Thus, mAb CO17-1A has the potential to act as an immune therapeutic protein against colorectal cancer. Recently, it was shown that the baculovirus insect cell expression system produces anti-colorectal cancer mAb CO17-1A. In this study, the colorectal cancer antibody mAb CO17-1A fused to the endoplasmic reticulum (ER) retention signal sequence (KDEL), and the (mAb CO17-1AK) was expressed in Spodoptera frugiperda Sf9 insect cells. The yield, cell cytotoxicity, and in vitro anti-tumor activity of mAb CO17-1AK were verified. Western blotting was performed to confirm that both heavy and light chains of mAb CO17-1A were expressed in Sf9 insect cells. The insect-derived mAb (mAbI ) CO17-1A was purified using a protein G affinity column. An in vitro wound healing assay was conducted to determine the inhibition activity of mAb CO17-1A during tumor cell migration, showing that mAbI CO17-1AK was effective as mammalian-derived mAb CO17-1A (mAbM CO17-1A). These results suggest that the insect cell expression system can produce and properly assemble mAbs that inhibit tumor cell migration.

Aetiology of cancer in Asia.

Park, Sohee,Bae, Jisuk,Nam, Byung-Ho,Yoo, Keun-Young Asian Pacific Organization for Cancer Prevention 2008 Asian Pacific journal of cancer prevention Vol.9 No.3

<P>Cancer has become the leading cause of death in many Asian countries. There is an increasing trend in breast, prostate and colon cancers, which are considered as typical of economically developed countries. Although breast and prostate cancer rates are still lower than in western countries, they are particularly rapidly increasing. In this paper, we review recently published literature to identify important etiologic factors affecting the cancer risk in Asian populations. Infectious agents such as Helicobacter pylori, hepatitis B and C viruses, and human papillomavirus were shown to be associated with elevated risks of stomach, liver and cervical cancer, respectively. Tobacco smoking was shown to be significantly associated with higher lung cancer risk and moderately increased all cancer risk. Excessive alcohol drinking appeared to increase the risk of colorectal cancer in Japanese and breast cancer in the Korean population. Betel nut chewing was associated with higher risk of oral and esophageal cancer. In terms of diet, various studies have demonstrated that high caloric and fat intake was associated with breast cancer risk, salted food intake with stomach cancer, aflatoxin B1 with liver cancer, and low fruits and vegetables intake with breast and lung cancer. Environmental exposure to indoor and outdoor air pollution, arsenic, radon, asbestos and second hand smoke was shown to increase the lung cancer risk. Reproductive factors such as late age at first childbirth, early menarche, late menopause, oral contraceptive intake, and short duration of lifetime lactation were shown to be associated with breast and/or colorectal cancer. Cancer has clearly become an emerging health threat in Asia and cancer control programs should be actively implemented and evaluated in this region. Various strategies for cancer control have been developed in some Asian countries, including the set-up of national cancer registries, cancer screening programs, education programs for health behavior change, eradication of Helicobacter pylori and vaccination for hepatitis B and C viruses, and human papilloma virus high risk forms. However, more attention should also be paid to low- and medium-resource Asian countries where cancer incidence rates are high, but neither intensive research on cancer for planning effective cancer control programs, nor easy implementation of such programs are available, due to limited financial resources.</P>

Absence of hMLH1 or hMSH2 Expression as a Stage-Dependent Prognostic Factor in Sporadic Colorectal Cancers

Park, Ji Won,Chang, Hee Jin,Park, Sohee,Kim, Byung Chang,Kim, Dae Yong,Baek, Ji-Yeon,Kim, Sun Young,Oh, Jae Hwan,Choi, Hyo Seong,Park, Sung Chan,Jeong, Seung-Yong Springer - Society of Surgical Oncology 2010 Annals of Surgical Oncology Vol.17 No.11

Photo-induced hybrid nanopatterning of titanium dioxide <i>via</i> direct imprint lithography

Park, Hyeong-Ho,Choi, Dae-Geun,Zhang, Xin,Jeon, Sohee,Park, Seong-Je,Lee, Soon-Won,Kim, Sarah,Kim, Ki-don,Choi, Jun-Hyuk,Lee, Jihye,Yun, Dae Keun,Lee, Ki Joong,Park, Hyung-Ho,Hill, Ross Henry,Jeong, J Royal Society of Chemistry 2010 Journal of materials chemistry Vol.20 No.10

<P>A novel ultraviolet (UV)-assisted imprinting procedure that employs photosensitive titanium(<SMALL>IV</SMALL>) di-n-butoxide bis(2-ethylhexanoate) is presented for the fabrication of well-ordered titanium dioxide (TiO<SUB>2</SUB>) nanostructures at room temperature. The main novelty of this technique is the use of the photosensitive titanium organic compound, rather than a commonly used UV-curable resin, for direct UV-assisted nanoimprint lithography. Fourier transform infrared and X-ray photoelectron spectroscopy studies suggest that exposure to UV light resulted in the gradual removal of organic groups from films prepared from titanium(<SMALL>IV</SMALL>) di-n-butoxide bis(2-ethylhexanoate) photochemically and successively converted the films to TiO<SUB>2</SUB> at room temperature. This approach allows direct fabrication of TiO<SUB>2</SUB> nanopatterns with lines down to 35 nm in width, hole arrays of 265 nm in diameter, and three-dimensional TiO<SUB>2</SUB> hybrid micro/nano-patterns without observable defects for use in applications where ordered surface nanostructures are required, such as photovoltaics, photonics, and optical waveguides.</P> <P>Graphic Abstract</P><P>A novel UV-assisted imprinting procedure that employs photosensitive Ti(<SMALL>IV</SMALL>) di-n-butoxide bis(2-ethylhexanoate) is presented for the fabrication of well-ordered TiO<SUB>2</SUB> and 3-D TiO<SUB>2</SUB> hybrid micro/nano-patterns at room temperature. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b921343k'> </P>

Adsorption of 1,3-Benzodithiolylium Tetrafluoroborate (1,3-BDYT) on Carbon Nanotubes

Sohee Park,Ki-jeong Kong,Hye-Mi So,이정오,장현주,Jaeyong Yee 한국물리학회 2010 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.57 No.1

We present systematic first-principles studies of the adsorption of 1,3-benzodithiolylium tetrafluoroborate (1,3-BDYT) on various single-walled carbon nanotubes (CNTs). 1,3-BDYT was reported as one of the electrophilic molecules that suppress the metallic properties of CNTs when it is adsorbed on the surfaces of CNTs. It was found that 1,3-BDYT tends to be more strongly bound to metallic CNTs of smaller diameters than to semiconducting CNTs. It interacts with CNTs via covalent sp3 bonding. From the band structure calculations, we found that 1,3-BDYT could open the band gap of the metallic CNTs by generating molecular states near the Fermi level via sp3hybridization.

The Gold of Industry and the Core of the Military: China’s Rare Earths Strategy and its Implications for Korea

Park Sohee 산업연구원 2023 KIET Industrial Economic Review Vol.28 No.4

MALDI-TOF Mass Spectrometry Based on Parylene-Matrix Chip for the Analysis of Lysophosphatidylcholine in Sepsis Patient Sera

Park, Jong-Min,Noh, Joo-Yoon,Kim, Moon-Ju,Yun, Tae Gyeong,Lee, Sang-Guk,Chung, Kyung Soo,Lee, Eun Hye,Shin, Mi Hwa,Ku, Nam Su,Yoon, Sohee,Kang, Min-Jung,Park, Moo Suk,Pyun, Jae-Chul American Chemical Society 2019 ANALYTICAL CHEMISTRY - Vol.91 No.22

<P>In this work, medical diagnosis of sepsis was conducted via quantitative analysis of lysophosphatidylcholine 16:0 (LPC 16:0) by using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry based on a parylene-matrix chip. In the first step, specific mass peaks for the diagnosis of sepsis were searched by comparing MALDI-TOF mass spectra of sepsis patient sera with healthy controls and pneumonia patient sera. Two mass peaks at <I>m</I>/<I>z</I> = 496.3 and 518.3 were chosen as those that are specifically different for sepsis sera to compare with healthy controls and pneumonia patient sera. These mass peaks were identified to be protonated and sodium adducts of LPC 16:0 by using tandem mass spectra (MS<SUP>2</SUP> and MS<SUP>3</SUP>) of purely synthesized LPC 16:0 and extracted LPC 16:0 from a healthy control and a sepsis patient. In the next step, a standard curve for LPC 16:0 for the quantitative analysis of LPC 16:0 with MALDI-TOF MS based on the parylene-matrix chip was prepared, and the statistical correlation to the LC-MS analysis results was demonstrated by using the Bland-Altman test and Passing-Bablok regression. Finally, MALDI-TOF MS based on the parylene-matrix chip was used for the quantification of LPC 16:0 with sera from patients with severe sepsis and septic shock (<I>n</I> = 143), pneumonia patients (<I>n</I> = 12), and healthy sera (<I>n</I> = 31). The sensitivity and the selectivity of medical diagnosis of sepsis was estimated to be 97.9% and 95.5% by using MALDI-TOF MS based on the parylene-matrix chip, respectively.</P> [FIG OMISSION]</BR>

Cutoff Values for Diagnosing Hepatic Steatosis Using Contemporary MRI-Proton Density Fat Fraction Measuring Methods

Park Sohee,Kwon Jae Hyun,김소연,Kang Ji Hun,Chung Jung Il,Jang Jong Keon,Jang Hye Young,Shim Ju Hyun,Lee Seung Soo,Kim Kyoung Won,Song Gi-Won 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.12

Objective: To propose standardized MRI-proton density fat fraction (PDFF) cutoff values for diagnosing hepatic steatosis, evaluated using contemporary PDFF measuring methods in a large population of healthy adults, using histologic fat fraction (HFF) as the reference standard. Materials and Methods: A retrospective search of electronic medical records between 2015 and 2018 identified 1063 adult donor candidates for liver transplantation who had undergone liver MRI and liver biopsy within a 7-day interval. Patients with a history of liver disease or significant alcohol consumption were excluded. Chemical shift imaging-based MRI (CS-MRI) PDFF and high-speed T2-corrected multi-echo MR spectroscopy (HISTO-MRS) PDFF data were obtained. By temporal splitting, the total population was divided into development and validation sets. Receiver operating characteristic (ROC) analysis was performed to evaluate the diagnostic performance of the MRI-PDFF method. Two cutoff values with sensitivity > 90% and specificity > 90% were selected to rule-out and rule-in, respectively, hepatic steatosis with reference to HFF ≥ 5% in the development set. The diagnostic performance was assessed using the validation set. Results: Of 921 final participants (624 male; mean age ± standard deviation, 31.5 ± 9.0 years), the development and validation sets comprised 497 and 424 patients, respectively. In the development set, the areas under the ROC curve for diagnosing hepatic steatosis were 0.920 for CS-MRI-PDFF and 0.915 for HISTO-MRS-PDFF. For ruling-out hepatic steatosis, the CS-MRI-PDFF cutoff was 2.3% (sensitivity, 92.4%; specificity, 63.0%) and the HISTO-MRI-PDFF cutoff was 2.6% (sensitivity, 88.8%; specificity, 70.1%). For ruling-in hepatic steatosis, the CS-MRI-PDFF cutoff was 3.5% (sensitivity, 73.5%; specificity, 88.6%) and the HISTO-MRI-PDFF cutoff was 4.0% (sensitivity, 74.7%; specificity, 90.6%). Conclusion: In a large population of healthy adults, our study suggests diagnostic thresholds for ruling-out and ruling-in hepatic steatosis defined as HFF ≥ 5% by contemporary PDFF measurement methods.

Attribution to Heterogeneous Risk Factors for Breast Cancer Subtypes Based on Hormone Receptor and Human Epidermal Growth Factor 2 Receptor Expression in Korea

Park, Boyoung,Choi, Ji-Yeob,Sung, Ho Kyung,Ahn, Choonghyun,Hwang, Yunji,Jang, Jieun,Lee, Juyeon,Kim, Heewon,Shin, Hai-Rim,Park, Sohee,Han, Wonshik,Noh, Dong-Young,Yoo, Keun-Young,Kang, Daehee,Park, Su Wolters Kluwer Health 2016 Medicine Vol.95 No.14

<▼1><P>Supplemental Digital Content is available in the text</P></▼1><▼2><P><B>Abstract</B></P><P>We conducted a heterogeneous risk assessment of breast cancer based on the hormone receptor (HR) and human epidermal growth factor receptor 2 (HER2) calculating the risks and population-based attributable fractions (PAFs) for modifiable and nonmodifiable factors.</P><P>Using matched case–control study design from the Seoul Breast Cancer Study and the national prevalence of exposure, the risks and PAFs for modifiable and nonmodifiable factors were estimated for total breast cancers and subtypes.</P><P>The attribution to modifiable factors was different for each subtype (luminal A, PAF = 61.4% [95% confidence interval, CI = 54.3%–69.8%]; luminal B, 21.4% [95% CI = 18.6–24.9%]; HER2-overexpression, 59.4% [95% CI = 47.8%–74.3%], and triple negative tumors [TNs], 27.1% [95% CI = 22.9%–32.4%)], and the attribution to the modifiable factors for the luminal A and HER2-overexpression subtypes was higher than that of the luminal B and TN subtypes (<I>P</I> heterogeneity ≤ 0.001). The contribution of modifiable reproductive factors to luminal A type in premenopausal women was higher than that of the other subtypes (18.2% for luminal A; 3.1%, 8.1%, and −3.1% for luminal B, HER2-overexpression, and TN subtypes, respectively; <I>P</I> heterogeneity ≤ 0.001). Physical activity had the highest impact preventing 32.6% of luminal A, 14.5% of luminal B, 38.0% of HER2-overexpression, and 26.9% of TN subtypes (<I>P</I> heterogeneity = 0.014). Total reproductive factors were also heterogeneously attributed to each breast cancer subtype (luminal A, 65.4%; luminal B, 24.1%; HER2-overexpression, 57.9%, and TN subtypes, −3.1%; <I>P</I> heterogeneity ≤ 0.001).</P><P>Each pathological subtype of breast cancer by HRs and HER2 status may be associated with heterogeneous risk factors and their attributable risk, suggesting a different etiology. The luminal B and TN subtypes seemed to be less preventable despite intervention for alleged risk factors, even though physical activity had a high preventable potential against breast cancer.</P></▼2>

Investingation of the shape of the dots in laser irradiated crystal and PMMA using optical coherence tomography

Sohee Park,Youngseop Kim,Yongjin Shin 한국레이저가공학회 2005 한국레이저가공학회지 Vol.8 No.2