生態親和的 飼育環境 制御方式 確立을 위한 누에 核多角體病 發生要因 分析

韓明世,林鐘聲 慶北大學校農業科學技術硏究所 1997 慶北大農學誌 Vol.15 No.-

世界的으로 養蠶地域에서 큰 피해를 주는 核多角體病은 養蠶現場에서 蠶室 蠶具類의 철저한 포르말린 소독에도 불구하고 예방효과가 疑問視되는 경우가 발생하며, 이는 병원체의 存否 만으로 본 疾病의 蔓延이 설명될 수 없기 때문이라는 사실을 實驗病理學的으로 立證하였다. 核多角體病의 發生을 助長하는 不良 環境要因을 파악하기 위하여 1979년부터 1993년 까지 15년간 한국 일본 필리핀 등지에서 57건의 사육사례를 조사하였다. 環境狀態와 發病事例의 연관성을 분석하므로써 發病生理와 관련된 環境要因을 해명한 결과, 고름병 發生頻度는 상엽육과 인공사료육 간에 비슷한 경향을 나타내었고, 잠실 잠구류의 消毒방법에서는 포르말린 消毒 與否에 따른 차이가 인정되지 않았다. 交雜種은 原種보다 核多角體病 발생율이 다소 높은 수준이었으며, 특히 애누에때 부터 장기간 通氣不良인 사육환경에서는 發病率이 현저하게 높았다. 또한, 1993년부터 1997년 까지 5년간의 實證試驗에서는 蠶室 및 蠶具類의 청소와 사육실의 强制循環式 換氣로 사육환경을 개선하므로써 人體에 有害한 농약을 사용하지 않고도 누에 核多角體病 발생을 효율적으로 제어할 수 있음을 立證하였다. For the prevention of worldwide prevalent disease of nuclear polyhedrosis virus (NPV), environmental conditions and their incidence of grasserie was investigated through 57 cases of silkworm rearing from the year of 1979 to 1993 in the countries of Korea, Japan, and Philippines. Relationship between the occurrence of NPV and environmental factors were also analysed from the aspect of causal pathogenesis. Unfavorable foactors related to the prevalence of NPV disease was reconfirmed by the assay of experimental rearing. Silkworms reared on mulberry leaves or artificial diet appeared similar result on the occurrence of grasserie. Disinfection by formalin and simple sweeping or washing was not significantly different on the occurrence of NPV disease. Following insufficient ventilation on the younger larvae, from the 1st to 3rd instar, the disease by NPV at the later stage was remarkably emphasized those insidence. An experimental rearing from 1993 to 1996 demonstrated the prevention of NPV disease by simple cleaning of sweeping under the condition of air forced ventilation, the customal practice of disinfection with formalin or any other chemical agents could be omissible.

Flutamide가 수컷 생쥐의 유해감각예민도에 미치는 영향

장세헌,김명정,박제민,양구범,한병득 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.1

Objectives : Previous findings on nociception modulatory effect of testosterone were controversial. In this study, androgen receptor antagonist flutamide was used to reveal testosterone effect on baseline nociceptive sensitivity and stress-induced antinociception in male mice. Methods : Experiments consist 3 Parts: 1) tail flick latency(TFL, sec, 52±1℃) was measured before and 30 minutes after intraperitoneal injection of flutamide 150, 100, 50mg/kg or vehicle(N=15 for each group); 2) TFL was measured before and after serial injection of LHRH antagonist antide(1mg/kg, ip), and 30 minutes after flutamide(100mg/kg, ip) or vehicle; 3) With pretreatment of flutamide(100mg/kg, ip) or vehicle(N=15 for each group), TFL was measured before and after 5 minutes of forced-swim in cold water(15±1℃). Results : 1.TFL was significantly elongated(hypoalgesic) in the flutamide 100 and 150mg/kg groups. TFL of flutamide 150mg/kg group was significantly longer than 50mg/kg group. 2.Though pretreatment with antide itself did not make any change in TFL, it blocked antinociceptive effect of flutamide. TFL was elongated by serial injections in both flutamide and control group. 3.TFL was elongated by FS in both flutamide and control group. FS-induced elongation of TFL was significantly more prominent in flutamide group. Conclusions : From these results, it is suggested flutamide has antinociceptive effect on baseline TFL and potentiates FS- induced antinociception.

家蠶에 寄生하는 Nuclear-Polyhedrosis Virus와 새로운 Microsporidia(Nosema sp. M_12)間의 相互作用

林鍾聲,韓明世 경북대학교 1981 새마을 硏究論叢 Vol.1 No.-

In the application of infectious microorganisms of insects to microbial control the interaction among those pathogens has been considered to be very important factors in causing synergism or intereference. In these respects this research was concentrated on the interaction between the nuclear polyhedrosis virus(NPV) and a newly isolated microsporidia, Nosema sp.(M_12) in the larvae of silkworm, Bombyx mori(L.), including comparisions of their pathogenecity and histopathological symptoms. The interference was observed between the two pathogens were incoculated per os to the second-instar larvae of the silkworm at the same time. In the response of the interaction the degree of interference by was greater than that by the NPV. However, the former pathognn M_12 was not interfered by the challanging NPV while the former NPV was considerably interfered by the challanging M_12 when they were inoculated per os at the intereval of 24 hours at the second-instar larvae. In the histopathological observations the sites of inteference were observed in some tissue like fat body or cell unit though one of the pathogens was dominant in each tissue. From the point of microbial control views it is neither recommendable to use NPV and M_12 at the same time nor the same areas in the case of both pathogens to be used as a microbial insecticide.

Establishment of Simplistic Moth Inspection System to Prevent Nosema bombycis Infection of the Silkworm, Bombyx mori

Han, Myung Sae,Mau Tuan Nguyen,Lim, Jong Sung 한국잠사학회 1997 한국잠사곤충학회지 Vol.39 No.2

Present experiment designed for the review of theoretical basis for the inspertion system of infected insects by Noema bombycis. A microsporidian N. bombycis, known as the high virulence, produced at the average of 7 X 108 spores per female moth of the silkworm, Bombyx mori, enabled transovarial tranmission. Detectability of N. bombycis spores in the mass inspection was varied by dillution level, the higher limit of dillution with healthy moths was 1:140 for 100% detection, 1:160 for 99.5%, 1:200 for 99.0%, For an efficient inspection under the microdcopic observation (600 X ), the lower limit of spore concentration was determined as 1,000,000 spores/ml, 60~80 moths could be applicable for a maximunl sample unit of a lot. Following the present inspection unit conditioned 35 to 40 moths for a lot. N. bombycis spores were easily detectable from the preparation of crude homogenate with 2% KOH, even the step of centrifuge was omitted. The results suggested a new basis of rational mass inspection system of silkworm female moths to save the facilities, labor, and time

누에 고치색 유전자 발현의 다양성 검정 및 색채과학적 해석

한명세 ( Myung Sae Han ),박현로 ( Hyun Ro Park ),한상미 ( Sang Mi Han ),남기수 ( Ki Soo Nam ),권순하 ( Soon Ha Kwon ),임종성 ( Jong Sung Lim ),( Mau Tuan Nguyen ) 한국잠사학회 1999 한국잠사곤충학회지 Vol.41 No.1

Microsporidia Identification of Characterization and DNA sequences in Bumble bees from Korea

Kyu-Won Kwak,Myung-Sae Han,Sung-Hee Nam,Ji-Young Choi,Wontae Kim,Seokhyun Lee,Kwan-Ho Park 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

The purpose of this stduy was to identify accurately and promptly the Microsporidia disease of bumble bees from Gangwon in Korea. Bumble bees are crucial pollinators of various crops and microsporidia are the critical infections of these hosts. Also, the various species of bumble bees have been used: Bombus terrestris, B. lucorum, B. occidentalis, B. ignites and B. impatiens etc. The symptoms of bumble bees infected microsporidia: their abdomens become distended, paralyzed and become sluggish and die early. We have identified the morphologies of the microsporidium by electron microscopy and found that the morphology of the microsporidium is rounded spore morph, with fairly small spores as described before in many other articles. For the specific and sensitive diagnosis of Nosema in bumble bees, we have developed the improved method of Polymerase Chain Reaction (PCR) conditions for expeditious diagnosis. Two pairs of primers were tested on N. bombi and the related microsporidia; Nosema apis and Nosema sp., both of which infect bumble bees and honey bees and further we have verified and analyzed DNA sequence data of N. bombi in bumble bees by using the BLAST server at the National Center for Biotechnology Information.

Simultaneous Detection of Insect Pathogen by multiplex PCR

Kyu-Won Kwak,Myung-Sae Han,Sung-Hee Nam,Ji-Young Choi,Seok-Hyun Lee,Hong-Geun Kim,Kwan-Ho Park 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.04

The purpose of this multiplex PCR assay is establishment and application for rapid and simultaneous detection of six pathogens related with insect diseases. Five pathogens were chosen based on the insect disease incidence rate in South Korea and specific primers of those pathogen were designed to detect insect diseases and test multiplex PCR for detecting Fungi; Beauveria bassiana(Bb), Metarhizium anisopliae(Ma), Bacteira; Bacillus thuringiensis(Bt), Pseudomonas aeruginosa(Pa), and Serratia marcescens(Sm). This research carried out the results detecting five kinds of insect pathogen of P. b. seulensis by multiplex PCR. Multiplex PCR is effective and save time to detect simultaneously these insect pathogens and multiple infections to prevent insect disease. In our study, using multiplex PCR, we demonstrated that P. b. seulensis was frequently infected with S. marcescens and co-infected with M. anisopliae in more than 80% of cases, indicating that such an analysis can be useful for pathogen identification, especially if different pathogens produce similar symptoms.

A Quantitative Evaluation of Nematodes in Cultivated Rhizosphere Soil

Han Sang-Mi,Baek Ha-Ju,Kim Moo-Sik,Han Myung-Sae 한국응용곤충학회 2003 한국응용곤충학회 학술대회논문집 Vol.2003 No.1

병리분야의 학술동향에 관한 사상적 배경과 발전 방안

한명세 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2

Nosema bombycis의 母體傳染에 관한 卵面 및 卵內 經路 檢定

韓明世 한국잠사학회 1996 한국잠사곤충학회지 Vol.38 No.1

Nosema bombycis was found on the both of surface and inside of the silkworm eggs layed by infected female moth, maternal-mediated transmission of those pathogens via the surface or internal site of silkworm eggs were investigated. .All of the meconia from infected female moths contained pathogenic spores, those concentration were 4.6(±0.24) X 106 /ml. The pathogens on the surface of nondiapause eggs were trnasmitted to the progeny larvae at the rate of 54.5%, however, lost their activity before hatching for the case of overwintered eggs or acid treatment (16% HCI at 46.2°C for 6 min). N. bombycis in the silkworm eggs was localized at the serosa, yolk, embryo except chorion tissue in the silkworm eggs layed by infected female moths. Development of the pathogens in the eggs was synchronized with embryogenesis, which secured the safety of pathogens against environmental condition, resulting in the high transmissibility of 91.2 ± 1.80% .