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      • KCI등재SCOPUS

        The Morpho-Somatic and Chromosomal Changes in Colchicine Polyploidy Induction Colocasia esculenta var. Antiquorium

        Feranita Haring(Feranita Haring ),Muh. Farid(Muh. Farid ),Sudirman Sudirman(Sudirman Sudirman ),Muhammad Fuad Anshori(Muhammad Fuad Anshori ) 한국육종학회 2023 Plant Breeding and Biotechnology Vol.11 No.2

        Increasing the productivity of Colocasia esculenta var. Antiquorum is very necessary to enhance the mass of tubers. This can be achieved through polyploid mutation method using colchicine to improve tuber size by increasing the number of chromosomes. Therefore, this study aimed to determine the effect of colchicine on morphology and polyploid changes of Colocasia esculenta var. Antiquorum at various concentrations and duration of immersion In vitro. The analysis was carried out as a completely randomized factorial design, with the first factor being colchicine concentration, consisting of 0.0%, 0.05%, 0.075%, and 0.1%. The second factor was the immersion time which consisted of 8 and 16 hours. The results showed that the best combination of concentration and immersion time on somatic morphology was 0.075% colchicine with 8 hours and 16 hours of immersion. Meanwhile, the best combination of chromosomal changes was 0.05% colchicine with 16 hours of immersion. These results indicated that the treatment of 0.05% colchicine concentration with a soaking time of 16 hours can be recommended for induction of polyploid mutations in Colocasia esculenta.

      • KCI등재

        Genetic Parameters and Multivariate Analysis to Determine Secondary Traits in Selecting Wheat Mutant Adaptive on Tropical Lowlands

        Muh Farid,Nasaruddin Nasaruddin,Yunus Musa,Muhammad Fuad Anshori,Ifayanti Ridwan,Jekvy Hendra,Gatot Subroto 한국육종학회 2020 Plant Breeding and Biotechnology Vol.8 No.4

        One of approaches to maintain the yield stability of the lowland tropical wheat is the use of secondary traits in theselection process. The identification of these characters requires a statistical approach in the form of genetic parameter analysis andmultivariate analysis. The objective of this study was to determine the secondary traits of adaptive wheat mutants in the lowlandsthrough the use of genetic parameters and multivariate analysis on the parameters. The study consisted of three field trials conducted inthree different regencies, namely Jeneponto (135 m above sea level (asl)), Maros (100 m asl) and Bantaeng (125 m asl). The study useda nested design, where replications were nested in the environments. The genotype factors consisted of 20 genotypes repeated threetimes. 11 characters were observed including vegetative and reproductive characters. The analysis used consisted of repeatability,correlation, cluster analysis, principal component analysis, factor analysis, and cross print analysis. The overall results of the analysisindicate that the number of productive tillers is the main secondary trait for the selection of adaptive wheat in the lowlands. Thecharacter can be recommended for selection criteria in testing wheat lines in the lowlands to make an effective selection.

      • KCI등재

        Image Based-Phenotyping and Selection Index Based on Multivariate Analysis for Rice Hydroponic Screening under Drought Stress

        ( Adinda Asri Laraswati ),( Rusnadi Padjung ),( Muh Farid ),( Nasaruddin Nasaruddin ),( Muhammad Fuad Anshori ),( Amin Nur ),( Andi Isti Sakinah ) 한국육종학회 2021 Plant Breeding and Biotechnology Vol.9 No.4

        The development of rice varieties that are tolerant of drought stress needs to be detected with Image-based phenotyping. This Image-based phenotyping method in combination with selection index and multivariate analysis can characterize the morphological response easily within a short time, which makes it is suitable for rice screening under drought stress. Therefore, this study aims to determine the selection index based on multivariate analysis and assess the effectiveness of using image-based phenotyping in drought rice screening. This study was conducted in two stages, the first was in static hydroponic and the second was in dynamic hydroponic. In static hydroponic, a split-plot design was used, where the levels of drought were the main plots and varieties were the subplots. However, in dynamic hydroponic, a nested design was used, where the replicates were nested in the drought level treatments. Also, The drought level factors used were PEG 0%, PEG 10%, and PEG 20%, and the variety factor consisted of 5 varieties which were repeated three times. The results showed that the selection index for static hydroponic consisted of shoot area (0.421), green shoot area (0.4177), and the area growth rate (0.4192). Meanwhile, the selection index in dynamic hydroponics consisted of object extent Y from the side (0.4516) and convex hull from the side (0.4177). The regression of the two-selection index has a good determination of 0.84. Hence, these results showed that rice screening based on image-based phenotyping can be recommended for rapid screening under drought stress.

      • KCI등재

        Seedling Stage Image-Based Phenotyping Selection Criteria through Tolerance Indices on Drought and Salinity Stress in Rice

        Andi Isti Sakinah,Muh Farid,Yunus Musa,Aris Hairmansis,Muhammad Fuad Anshori 한국육종학회 2024 Plant Breeding and Biotechnology Vol.12 No.-

        The development of rice seedlings stressed by drought and salt is shown by different morphometric and colorimetric traits. These distinctions can be used to understand the response of plants to challenging conditions. Therefore, this study aimed to assess the efficacy of image-based phenotyping in the early testing of rice plants and observe how the plants respond to both drought and salinity. A stress tolerance index with multivariate analysis was used for the selection of the most important traits. The experiment consisted of 2 factors, namely the degree of environmental stress and rice genotype. Furthermore, the degree of environmental stress comprised normal (NaCl and PEG 0%), drought (10% PEG), salinity (60 mM NaCl), as well as a combination of moderate drought and salinity (5% PEG + 30 mM NaCl). The results showed that both morphometric (area, convex hull, bounding area, perimeter, centermassy) and colorimetric (CIVE, VARI, RGBVI, MGRVI, NDI, GLI, NGRDI) can be used as selection characters.

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