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RISS 인기검색어
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- 저자 : ( Mana Kumazawa ) (검색결과 1 건)
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Hypoxia Tolerance Evaluation of Red Leaf Lettuce by Expression Analysis of LsADH Gene
( Mana Kumazawa ),( Manasikan Thammawong ),( Hushna Ara Naznin ),( Kohei Nakano ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
The aim of this study was to evaluate the hypoxia tolerance of fresh produce by gene expression analysis. An alcohol dehydrogenase-encoding gene (ADH), which is a key factor determining anaerobic metabolism, was analyzed in red leaf lettuce stored at 25 ºC under various O<sub>2</sub> conditions. LsADH expression of samples stored under 15%, 10% and 3.5% O<sub>2</sub> condition was detected about 6 times higher than the control (20.9%), and it was remarkably increased by 12 times when stored under 2% O<sub>2</sub>. On the other hand, the respiratory quotient breakpoint (RQB) was determined as 0.3% because RQ was mostly stable at around 1.1 under the O<sub>2</sub> level ranged from 20.9% to 0.3%, and notably increased at below 0.3%. The fermentation threshold (FT) was found as 1% because ethanol and acetaldehyde were remarkably accumulated at below 1%O<sub>2</sub>. In this study, 2% O<sub>2</sub> at which the relative gene expression level of LsADH increased markedly was considered as the gene expression breakpoint (GEB). From this fact, the ‘low O<sub>2</sub> limit’, estimated based on GEB is relatively higher and more sensitive compared to RQB and FT methods. GEB is thought to be a useful indicator in determining the appropriate O<sub>2</sub> concentration for storing fresh produce.
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