Identification and quantification of oleanane triterpenoid saponins and potential analgesic and anti-inflammatory activities from the roots and rhizomes of Panax stipuleanatus

Pan-Pan Shu,Lu-Xi Li,Qin-Min He,Jun Pan,Xiao-Lei Li,Min Zhu,Ye Yang,Yuan Qu 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.2

Background: Panax stipuleanatus represents a folk medicine for treatment of inflammation. However, lack of experimental data does not confirm its function. This article aims to investigate the analgesic and anti-inflammatory activities of triterpenoid saponins isolated from P. stipuleanatus. Methods: The chemical characterization of P. stipuleanatus allowed the identification and quantitation of two major compounds. Analgesic effects of triterpenoid saponins were evaluated in two models of thermal- and chemical-stimulated acute pain. Anti-inflammatory effects of triterpenoid saponins were also evaluated using four models of acetic acid-induced vascular permeability, xylene-induced ear edema, carrageenan-induced paw edema, and cotton pellet-induced granuloma in mice. Results: Two triterpenoid saponins of stipuleanosides R₁ (SP-R₁) and R₂ (SP-R₂) were isolated and identified from P. stipuleanatus. The results showed that SP-R₁ and SP-R₂ significantly increased the latency time to thermal pain in the hot plate test and reduced the writhing response in the acetic acid-induced writhing test. SP-R₁ and SP-R₂ caused a significant decrease in vascular permeability, ear edema, paw edema, and granuloma formation in inflammatory models. Further studies showed that the levels of inflammatory mediators, nitric oxide, malondialdehyde, tumor necrosis factor-α, and interleukin 6 in paw tissues were downregulated by SP-R₁ and SP-R₂. In addition, the rational harvest of three- to five-year-old P. stipuleanatus was preferable to obtain a higher level of triterpenoid saponins. SP-R₂ showed the highest content in P. stipuleanatus, which had potential as a chemical marker for quality control of P. stipuleanatus. Conclusion: This study provides important basic information about utilization of P. stipuleanatus resources for production of active triterpenoid saponins.

Research on Integration Algorithm of Global Function Call Path Based on Module Path

Pan Lu,Mu Yong-min,Yang Zhi-jia 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.6

Numerical simulation of tip clearance impact on a pumpjet propulsor

Lu, Lin,Pan, Guang,Wei, Jing,Pan, Yipeng The Society of Naval Architects of Korea 2016 International Journal of Naval Architecture and Oc Vol.8 No.3

Numerical simulation based on the Reynolds Averaged Naviere-Stokes (RANS) Computational Fluid Dynamics (CFD) method had been carried out with the commercial code ANSYS CFX. The structured grid and SST $k-{\omega}$ turbulence model had been adopted. The impact of non-condensable gas (NCG) on cavitation performance had been introduced into the Schnerr and Sauer cavitation model. The numerical investigation of cavitating flow of marine propeller E779A was carried out with different advance ratios and cavitation numbers to verify the numerical simulation method. Tip clearance effects on the performance of pumpjet propulsor had been investigated. Results showed that the structure and characteristics of the tip leakage vortex and the efficiency of the propulsor dropped more sharply with the increase of the tip clearance size. Furthermore, the numerical simulation of tip clearance cavitation of pumpjet propulsor had been presented with different rotational speed and tip clearance size. The mechanism of tip clearance cavitation causing a further loss of the efficiency had been studied. The influence of rotational speed and tip clearance size on tip clearance cavitation had been investigated.

100KW DC Arc Plasma of CVD System for Low Cost Large Area Diamond Film Deposition

Lu, F.X.,Zhong, G.F.,Fu, Y.L.,Wang, J.J.,Tang, W.Z.,Li, G.H.,Lo, T.L.,Zhang, Y.G.,Zang, J.M.,Pan, C.H.,Tang, C.X.,Lu, Y.P. The Korean Ceramic Society 1996 The Korean journal of ceramics Vol.2 No.4

In the present paper, a new type of DC arc plasma torch is disclosed. The principles of the new magnetic and fluid dynamic controlled large orifice long discharge tunnel plasma torch is discussed. Two series of DC Plasma Jet diamond film deposition equipment have been developed. The 20kW Jet equipped with a $\Phi$70 mm orifice torch is capable of deposition diamond films at a growth rate as high as 40$\mu\textrm{m}$/h over a substrate area of $\Phi$65 mm. The 100kW high power Jet which is newly developed based on the experience of the low power model is equipped with a $\Phi$120 mm orifice torch, and is capable of depositing diamond films over a substrate area of $\Phi$110 mm at growth rate as high as 40 $\mu\textrm{m}$/h, and can be operated at gas recycling mode, which allows 95% of the gases be recycled. It is demonstrated that the new type DC plasma torch can be easily scaled up to even higher power Jet. It is estimated that even by the 100kW Jet, the cost for tool grade diamond films can be as low as less than $4/carat.

p13 from group II baculoviruses is a killing-associated gene

( Nan Lu ),( En Qi Du ),( Yang Kun Liu ),( Hong Qiao ),( Lun Guang Yao ),( Zi Shu Pan ),( Song Ya Lu ),( Yi Peng Qi ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.12

p13 gene was first described in Leucania separata multinuclear polyhedrosis virus (Ls-p13) several years ago, but the function of P13 protein has not been experimentally investigated to date. In this article, we indicated that the expression of p13 from Heliothis armigera single nucleocapsid nucleopolyhedrovirus (Ha-p13) was regulated by both early and late promoter. Luciferase assay demonstrated that the activity of Ha-p13 promoter with hr4 enhancer was more than 100 times in heterologous Sf9 cells than that in nature host Hz-AM1 cells. Both Ls-P13 and Ha-P13 are transmembrane proteins. Confocal microscopic analysis showed that both mainly located in the cytoplasm membrane at 48 h. Results of RNA interference indicated that Ha-p13 was a killing-associated gene for host insects H. armigera. The AcMNPV acquired the mentioned killing activity and markedly accelerate the killing rate when expressing Ls-p13. In conclusion, p13 is a killing associated gene in both homologous and heterologous nucleopolyhedrovirus. [BMB Reports 2012; 45(12): 730-735]

Numerical simulation of tip clearance impact on a pumpjet propulsor

Lin Lu,Guang Pan,Jing Wei,Yipeng Pan 대한조선학회 2016 International Journal of Naval Architecture and Oc Vol.8 No.3

Numerical simulation based on the Reynolds Averaged NaviereStokes (RANS) Computational Fluid Dynamics (CFD) method had been carried out with the commercial code ANSYS CFX. The structured grid and SST keu turbulence model had been adopted. The impact of noncondensable gas (NCG) on cavitation performance had been introduced into the Schnerr and Sauer cavitation model. The numerical investigation of cavitating flow of marine propeller E779A was carried out with different advance ratios and cavitation numbers to verify the numerical simulation method. Tip clearance effects on the performance of pumpjet propulsor had been investigated. Results showed that the structure and characteristics of the tip leakage vortex and the efficiency of the propulsor dropped more sharply with the increase of the tip clearance size. Furthermore, the numerical simulation of tip clearance cavitation of pumpjet propulsor had been presented with different rotational speed and tip clearance size. The mechanism of tip clearance cavitation causing a further loss of the efficiency had been studied. The influence of rotational speed and tip clearance size on tip clearance cavitation had been investigated.

다목적 실용위성 해색센서 (OSMI)의 복사영상에 대한 모의 및 평가

반덕로 ( De Lu Pan ),김용승 ( Yong Seung Kim ) 大韓遠隔探査學會 1999 大韓遠隔探査學會誌 Vol.15 No.2

가시광선 위성자료는 해색 연구에 성공적으로 응용되어 왔다. 또 하나의 해색관측 센서인 OSMI가 다목적 실용위성에 탐재되어 1999년 발사될 예정이다. 향후 OSMI 영상특성을 이해하기 위해서 우선 모의실험 절차를 자세히 논의하고 복사전달 모델을 이용하여 전형적인 경우의 가시대역 복사패턴을 모의 생성시켰다. 각 성분의 복사량 (에어로졸 산란, Rayleigh 산란, 태양복사, 해수 상향 복사량 및 총 복사량) 분포를 설명하기 위해 계절별, 해수형태 및 위성의 적도 통과 시각에 대한 위성의 전체 통과 경로와 한국 근해지역의 여러 가지 모의 영상들이 소개된다. 그리고 영상의 품질 및 가용도를 평가하기 위한 방법은 복합신호잡음비(CSNR)로 정의된 영상특성을 이용해 개발된다. 한편, 다목적 실용위성이 궤도에 진입 되기 전에 OSMI 영상자료의 질과 가용도를 평가할 수 있도록 일련의 CSNR영상이 서로 다른 경우에 대한 모의 복사량 성분들로부터 생성된다. 최종적으로 OSMI영상의 질과 가용도가 CSNR모의 영상을 이용해 정량적으로 분석된다. 본 연구결과가 OSMI 임무를 책임지고 있는 모든 과학자들과 OSMI 자료활용을 계획하고 있는 사람들에게 유용하게 되기를 기대한다. The satellite visible data have been successfully applied to study the ocean color. Another ocean color sensor, the Ocean Scanning Multi-spectral Imager (OSMI) on the Korea Multi-Purpose Satellite (KOMPSAT) will be launched in 1999. In order to understand the characteristics of future OSMI images, we have first discussed the simulation models and procedures in detail, and produced typical patterns of radiances at visible bands by using radiative transfer models. The various simulated images of full satellite passes and Korean local areas for different seasons, water types, and the satellite crossing equator time (CET) are presented to illustrate the distribution of each component of radiance (i.e., aerosol scattering, Rayleigh scattering, sun glitter, water-leaving radiance, and total radiance). A method to evaluate the image quality and availability is then developed by using the characteristics of image defined as the Complex Signal Noise Ratio (CSNR). Meanwhile, a series of CSNR images are generated from the simulated radiance components for different cases, which can be used to evaluate the quality and availability of OSMI images before the KOMPSAT will be placed in orbit. Finally, the quality and availability of OSMI images are quantitatively analyzed by the simulated CSNR image. It is hoped that the results would be useful to all scientists who are in charge of OSMI mission and to those who plan to use the data from OSMI.

Trajectory Optimization for Spacecraft Using a Second-Order Gauss Pseudo Spectral Method

Guoyong Li,Changhou Lu,Wei Pan,Qing Wang 보안공학연구지원센터(IJUNESST) 2015 International Journal of u- and e- Service, Scienc Vol.8 No.1

An optimal control problem for the trajectory of spacecraft under nonlinear constraints is presented. The second-order Gauss pseudo spectral method(GPM) is described for solving the problem numerically. Based on the first-order GPM, the differential matrix of the second-order GPM is derived. The dynamic equations and its constraints are approximated by the two methods. The terminal state constraints of the second-order GPM are converted to two constraints: terminal state variables and the first-order derivative constraints. The first-order GPM and second-order GPM are compared in terms of the accuracy of the state and control, the number of variables in NLP, the convergence time. A key feature of the second-order method is that it provides a more accurate and efficient way than the first-order method, due to reducing the number of variables in the nonlinear programming problem that is transcribed. A numerical example is used to identify the key differences between the two methods. The results of this study indicate that the first-order and second-order Gauss methods are very similar in accuracy, while the computational efficiency of the two methods is significantly different, for the numerical solution of nonlinear optimal control problems.

Simultaneous treatment with sorafenib and glucose restriction inhibits hepatocellular carcinoma in vitro and in vivo by impairing SIAH1-mediated mitophagy

Zhou Jing,Feng Ji,Wu Yong,Dai Hui-Qi,Zhu Guang-Zhi,Chen Pan-Hong,Wang Li-Ming,Lu Guang,Liao Xi-Wen,Lu Pei-Zhi,Su Wen-Jing,Hooi Shing Chuan,Ye Xin-Pin,Shen Han-Ming,Peng Tao,Lu Guo-Dong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Transarterial chemoembolization (TACE) is the first-line treatment for unresectable intermediate-stage hepatocellular carcinoma (HCC). It is of high clinical significance to explore the synergistic effect of TACE with antiangiogenic inhibitors and the molecular mechanisms involved. This study determined that glucose, but not other analyzed nutrients, offered significant protection against cell death induced by sorafenib, as indicated by glucose deprivation sensitizing cells to sorafenib-induced cell death. Next, this synergistic effect was found to be specific to sorafenib, not to lenvatinib or the chemotherapeutic drugs cisplatin and doxorubicin. Mechanistically, sorafenib-induced mitophagy, as indicated by PINK1 accumulation, increased the phospho-poly-ubiquitination modification, accelerated mitochondrial membrane protein and mitochondrial DNA degradation, and increased the amount of mitochondrion-localized mKeima-Red engulfed by lysosomes. Among several E3 ubiquitin ligases tested, SIAH1 was found to be essential for inducing mitophagy; that is, SIAH1 silencing markedly repressed mitophagy and sensitized cells to sorafenib-induced death. Notably, the combined treatment of glucose restriction and sorafenib abolished ATP generation and mitophagy, which led to a high cell death rate. Oligomycin and antimycin, inhibitors of electron transport chain complexes, mimicked the synergistic effect of sorafenib with glucose restriction to promote cell death mediated via mitophagy inhibition. Finally, inhibition of the glucose transporter by canagliflozin (a clinically available drug used for type-II diabetes) effectively synergized with sorafenib to induce HCC cell death in vitro and to inhibit xenograft tumor growth in vivo. This study demonstrates that simultaneous treatment with sorafenib and glucose restriction is an effective approach to treat HCC, suggesting a promising combination strategy such as transarterial sorafenib-embolization (TASE) for the treatment of unresectable HCC.

Endochronic prediction for the mechanical ratchetting of a stepped beam subjected to steady tension and cyclic bending

Pan, W. F.,Yang, Y. S.,Lu, J. K. Techno-Press 1998 Structural Engineering and Mechanics, An Int'l Jou Vol.6 No.3

In this paper, the first-order ordinary differential constitutive equations of endochronic theory are incorporated into finite element formalism. A theoretical investigation is performed on the ratchetting effect of a stepped beam subjected to steady tension and cyclic bending. Experimental data of lead alloy found in literature are used for comparison. Those data reveal that the endochronic prediction yields more adequate results than those predictions using the plasticity models with isotropic hardening or kinematic hardening, as employed by Hardy, et al. (1985).