울산연안 定置網에 어획된 漁類의 종조성 및 양적변동

한경호,김종헌,백승록 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.1

울산 정자연안 외측에 위치한 정치망에서 1998년 2월, 4월 ,6월 ,8월 10월 및 12월, 총 6회에 걸쳐 어획물을 채집하여, 魚類의 種組成, 量的變動 및 群集構造에 대하여 연구하였다. 채집된 어류는 총 12目 45科 89種, 62,978개체였고, 총 생체량은 6,494㎏이였다. 농어目 어류와 쏨뱅이目 어류가 총 56種으로 전체 出現種數의 62.9%를 차지하여 가장 우점하는 目들로 나타났으며, 科別로는 전갱이科와 참복科 어류가 각각 7種 出現하여 가장 많은 種이 出現하였다. 총 89種의 어류중 個體數는 고등어, 전갱이, 멸치 및 말쥐치의 4種이 全個體數의 89.7%를 차지하여 우점하였다. 生體量은 고등어, 전갱이, 꽁치, 말쥐치, 전어, 꼬치고기, 멸치, 정어리, 임연수어 및 덕대의 順으로 이들 10種이 全生體量의 95.5%를 차지하여 우점하였다. 出現 個體數와 生體量은 고수온기인 여름에 높게 나타났으며, 저수온기인 봄과 겨울에 낮게 나타나 수온과 밀접한 관계가 있었다. 월별 出現種數는 2월과 10월에 비교적 적은 種이 출현하였으며, 4월에 49種, 6월에 50種이 나타나 가장 많은 種이 출현하였고, 8월에 46種, 12월에 44種이 出現하였다. 종다양도지수(H')와 0.693~2.146으로 4월에 가장 높은 값을 보였고, 8월에 가장 낮은 값을 보였으며, 우점도지수는 다양도지수와 반대로 4월에 가장 낮은 값을 8월에 가장 높았다. 주요 魚種의 出現頻度를 보면 고등어, 전갱아. 멸치, 말쥐치, 꽁치, 전어, 망상어 및 정어리가 거의 年中 出現하여 본 해역에서의 주거종이었다. Fishes were collected by set net to determine seasonal species composition and fluctuation in abundance in coastal waters of Ulan. Korea, in February, April, June, August and December, 1998. A total of 62,978 fishes (6,494㎏) were sampled and classified into 12 orders, 45 families and 89 species. The most dominant orders were Perciformes and Scorpaeniformes, which accounted for 62.9% of the total. The most dominant families were Carangidae and Tetraodontidae, each represented seven species. The most dominant species were Scomber japonicus, Trachurus japonicus, Engraulis japonicus and Maurolicus muelleri, which accounted for 89.7% of the total fishes collected. The most dominant in biomass were Scomber japonicus, Trachurus japonicus, Cololabis saira, Thamnaconus modestus, Konosirus punctatus, Sphyraena pingues, Engraulis japonicus, Sardinops melanostictus, Pleurogrammus azonus and Pampus echinogaster. Those species together accounted for 95.5% of the total biomass. The number of individuals and the biomass were much higher in summer than in spring and winter, and closely correlated with water temperature. The number of species showed a peak in August, with 46 species, and the lowest number in February, with 30 species. The diversity index was the highest in April(H'=2.146) and the lowest in August(H'=0.693).

Nicotinamide phosphoribosyltransferase regulates the cell differentiation and mineralization in cultured odontoblasts

Kyeong-Rok Kang,Jae-Sung Kim,Jeong-Yeon Seo,HyangI Lim,Tae-Hyeon Kim,Sun-Kyoung Yu,Heung-Joong Kim,Chun Sung Kim,Hong Sung Chun,Joo-Cheol Park,Do Kyung Kim 대한생리학회-대한약리학회 2022 The Korean Journal of Physiology & Pharmacology Vol.26 No.1

The aim of the present study was to investigate the physiological role of nicotinamide phosphoribosyltransferase (NAMPT) associated with odontogenic differentiation during tooth development in mice. Mouse dental papilla cell-23 (MDPC- 23) cells cultured in differentiation media were stimulated with the specific NAMPT inhibitor, FK866, and Visfatin (NAMPT) for up to 10 days. The cells were evaluated after 0, 4, 7, and 10 days. Cell viability was measured using the 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide assay. The mineralization assay was performed by staining MDPC-23 cells with Alizarin Red S solution. After cultivation, MDPC-23 cells were harvested for quantitative PCR or Western blotting. Analysis of variance was performed using StatView 5.0 software (SAS Institute Inc., Cary, NC, USA). Statistical significance was set at p < 0.05. The expression of NAMPT increased during the differentiation of murine odontoblast-like MDPC-23 cells. Furthermore, the up-regulation of NAMPT promoted odontogenic differentiation and accelerated mineralization through an increase in representative odontoblastic biomarkers, such as dentin sialophosphoprotein, dentin matrix protein-1, and alkaline phosphatase in MDPC-23 cells. However, treatment of the cells with the NAMPT inhibitor, FK866, attenuated odontogenic differentiation, as evidenced by the suppression of odontoblastic biomarkers. These data indicate that NAMPT regulated odontoblastic differentiation through the regulation of odontoblastic biomarkers. The increase in NAMPT expression in odontoblasts was closely related to the formation of the extracellular matrix and dentin via the Runx signaling pathway. Therefore, these data suggest that NAMPT is a critical regulator of odontoblast differentiation during tooth development.

Inhibition of cell growth and induction of apoptosis by acacetin in FaDu human pharyngeal carcinoma cells

Kyeong-Rok Kang,Jae-Sung Kim,Tae-Hyeon Kim,Jeong-Yeon Seo,Jong-Hyun Park,Jin Woong Lim,Sun-Kyoung Yu,Heung-Joong Kim,Sang Hun Shin,Bo-Ram Park,Chun Sung Kim,Do Kyung Kim 대한구강생물학회 2020 International Journal of Oral Biology Vol.45 No.3

Acacetin, which is present in damiana (Turnera diffusa ) and black locust (Robinia pseudoacacia ), has several pharmacologic activities such as antioxidant, anti-inflammatory, and anti-proliferative effects on cancer cells. However, the effect of acacetin on head and neck cancers has not been clearly established. This study aimed to examine the effects of acacetin on cell growth and apoptosis induction in FaDu human pharyngeal carcinoma cells. These were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, Live/Dead cell assay, 4′,6-diamidino-2-phenylindole dihydrochloride staining, caspase-3 and caspase-7 activation assay, and immunoblotting in FaDu cells. Acacetin induced FaDu cell death in a dose-dependent manner, with an estimated IC50 value of 41.9 µM, without affecting the viability of L-929 mouse fibroblasts as normal cells. Acacetin treatment resulted in nuclear condensation in the FaDu cells. It promoted the proteolytic cleavage of procaspase-3, -7, -8, and -9 with increasing amounts of the cleaved caspase isoforms in FaDu cells. Acacetin-induced apoptosis in FaDu cells was mediated by the expression of Fas and activation of caspase-8, caspase-3, and poly (ADP-ribose) polymerase. Immunoblotting showed downregulation of the anti-apoptotic mitochondrial proteins Bcl-2 and Bcl-xL, but upregulation of the mitochondria-dependent pro-apoptotic proteins Bax and Badin FaDu cells after acacetin treatment. These findings indicate that acacetin inhibits cell proliferation and induces apoptotic cell death in FaDu human pharyngeal carcinoma cells via both the death receptor-mediated extrinsic apoptotic pathway and the mitochondria-mediated intrinsic apoptotic pathway.

Synthesis and Characterization of β-Tricalcium Phosphate Derived From Haliotis sp. Shells :

Kang, Kyeong-Rok,Piao, Zheng-Gang,Kim, Jae-Sung,Cho, In-A,Yim, Min-Ji,Kim, Bok-Hee,Oh, Ji-Su,Son, Jun Sik,Kim, Chun Sung,Kim, Do Kyung,Lee, Sook-Young,Kim, Su-Gwan Ovid Technologies (Wolters Kluwer) - Lippincott Wi 2017 Implant dentistry Vol.26 No.3

<P>Conclusions: beta-TCP (Ca-3(PO4)(2)) synthesized from abalone shell can be used as a potential source of bone grafting material.</P>

X-band SAR 파형발생기 측정 및 분석

김경록(Kyeong-Rok Kim),천혜림(Hye-Rim Cheon),김진기(Jin-Ki Kim),김송(Song Kim),김재현(Jae-Hyun Kim) 한국통신학회 2019 한국통신학회 학술대회논문집 Vol.2019 No.6

Apoptotic activity of demethoxycurcumin in MG-63 human osteosarcoma cells

Kang, Kyeong-Rok,Kim, Jae-Sung,Kim, Tae-Hyeon,Seo, Jeong-Yeon,Park, Jong-Hyun,Chun, Hong Sung,Yu, Sun-Kyoung,Kim, Heung-Joong,Kim, Chun Sung,Kim, Do Kyung The Korean Academy of Oral Biology 2021 International Journal of Oral Biology Vol.46 No.1

Demethoxycurcumin (DMC), which is a curcuminoid found in turmeric, has anti-proliferative effects on cancer cells. However, the effect of DMC on osteosarcoma has not been established. The aim of this study was to examine the effects of DMC on cell growth and apoptosis induction in MG-63 human osteosarcoma cells. This study was investigated using 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromid assay, Live/Dead cell assay, 4', 6-diamidino-2-phenylindole staining, and immunoblotting in MG-63 cells. DMC induced MG-63 cell death in a dose-dependent manner, with an estimated IC50 value of 54.4 µM. DMC treatment resulted in nuclear condensation in MG-63 cells. DMC-induced apoptosis in MG-63 cells was mediated by the expression of Fas and activation of caspase-8, caspase-3, and poly (ADP-ribose) polymerase. Immunoblotting results showed that Bcl-2 and Bcl-xL were downregulated, while Bax and Bad were upregulated by DMC in MG-63 cells. These results indicated that DMC inhibits cell proliferation and induces apoptotic cell death in MG-63 human osteosarcoma cells via the death receptor-mediated extrinsic apoptotic pathway and mitochondria-mediated intrinsic apoptotic pathway.

Bioinspired Synthesis of Hierarchical Silica Nanocage Using an Engineered Capsid Protein

Kyeong Rok KIM,Chang Sup KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

Silica nanoparticles have received increasing attention as an ideal candidate for biomedical application due to their excellent biocompatibility, chemical stability, easy surface modification, and high loading capacity. Nano-particles with hierarchical surfaces have tremendous advantages compared to flat surfaces as a carrier in biomedical applications, including large surface area, prolonged blood circulation time, and high binding efficiency to cancer cells, compared to flat nanoparticles. To date, chemical synthesis method has been used to make hierarchical silica nanoparticles, but this method has some limitations, such as the use of harsh conditions, the difficulty of removing a surfactant, and the complicated process. In this study, we have developed silica nanoparticles with the hierarchical surface through a combination of two bioinspired approaches, controlled silica mineralization of diatom and hierarchical structure of human papillomavirus (HPV) 16. A novel fusion protein, HPV 16 L1-R5, was prepared by genetically inserted HPV 16 capsid protein L1 with silica-forming R5 peptide from a diatom Cylindrotheca fusiformis. HPV16 L1-R5 proteins self-assembled into virus-like particles (VLPs) identical with native HPV 16. Hierarchical HPV16 L1-R5 VLP@SiNPs were developed with a size of ca. 60-80 ㎚ under an optimized condition of diatom-inspired silicification. HPV16 L1-R5 VLP@SiNPs encapsulated therapeutic compound and exhibited high cellular uptake efficiency compared to flat mesoporous SiNPs. We expect that our silica nanoparticle with a hierarchical surface could be used as an effective carrier for biomedical application.

Determination of Terbutaline Enantiomers in Human Urine by Capillary Electrophoresis Using $Hydroxypropyl-{\beta}-cyclodextrin$ as a Chiral Selector

Kim, Kyeong-Ho,Seo, Sang-Hun,Kim, Hyun-Ju,Jeun, Eun-Young,Kang, Jong-Seong,Mar, Woong-Chon,Youm, Jeong-Rok The Pharmaceutical Society of Korea 2003 Archives of Pharmacal Research Vol.26 No.2

A method for the determination of terbutaline enantiomers in human urine by capillary elctrophoresis has been developed. Optimum resolution was achieved using 50 mM phosphate buffer, pH 2.5, containing 15 mM of hydroxypropyl-$\beta$-cyclodextrin as a chiral selector. Urine samples were prepared by solid-phase extraction with Sep-pak silica, followed by CE. The assay was linear between 2-250 ng/mL (R = 0.9998 for (S)-(+)-terbutaline and R = 0.9999 for (R)-(-)-terbutaline) and detection limit was 0.8 ng/mL. The intra-day variation ranged between 6.3 and 14.5% in relation to the measured concentration and the inter-day variation was 8.2-20.1%. It has been applied to the determination of (S)-(+)-terbutaline and (R)-(-)-terbutaline in urine from healthy volunteer dosed with racemic terbutaline sulfate.

Diatom-inspired Synthesis of Silica Nanocages with the Hierarchical Surface Using Engineered Capsid Proteins as a Carrier for Drug Delivery

Kyeong Rok KIM,Chang Sup KIM 한국생물공학회 2023 한국생물공학회 학술대회 Vol.2023 No.4

Improvement in methanogenesis by incorporating transition metal nanoparticles and granular activated carbon composites in microbial electrolysis cells

Kim, Kyeong-Rok,Kang, Jun,Chae, Kyu-Jung Pergamon Press 2017 International journal of hydrogen energy Vol.42 No.45

<P><B>Abstract</B></P> <P>Electromethanogenesis is a form of electrobiofuel production through a microbial electrolysis cell (MEC) where methane (CH<SUB>4</SUB>) is directly produced from an electrical current and carbon dioxide (CO<SUB>2</SUB>) using a cathode. With the aim of maximizing methanogenesis in an MEC, this study utilized granular activated carbon (GAC) and a transition metal catalyst to fabricate nickel (Ni) nanoparticle (NP)-loaded GAC (NiNP/GAC) composites and incorporated these into MECs. In this set-up, GAC acted as the main electrical conduit for direct interspecies electron transfer (DIET) between exoelectrogens and methanogenic electrotrophs, and the Ni NPs served as a catalyst to further improve microbe-to-GAC electron transfer. The NiNP/GAC-composites were prepared using two different methods (microwave irradiation and solution plasma ionization). The Ni NPs were determined to be well doped on the GAC surface according to a field emission scanning electron microscope (FE-SEM) and energy-dispersive X-ray (EDX) spectroscopy analysis. Adding GAC into MECs improved CH<SUB>4</SUB> production. The NiNP/GAC composites prepared by solution plasma ionization showed the highest CH<SUB>4</SUB> production (20.7 ml), followed by the NiNP/GAC composite prepared by microwave irradiation (19.6 ml), bare GAC (15.6 ml), and GAC-free control (9.6 ml). In the methanogenic MECs, 40.6% of CH<SUB>4</SUB> was produced from an electrode reaction (i.e., reduction of CO<SUB>2</SUB> to CH<SUB>4</SUB>), and the remaining 59.4% was generated by nonelectrode reactions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> To maximize electromethanogenesis, Ni nanoparticles were doped on granular activated carbon (GAC). </LI> <LI> Ni nanoparticle-loaded GAC composites (NiNP/GAC) were synthesized and incorporated into MECs. </LI> <LI> NiNP/GAC-composites were prepared by the microwave irritation and solution plasma method. </LI> <LI> Incorporating NiNP/GAC-composites into MECs improved the methane production. </LI> <LI> In the methanogenic-MECs, 40.6% of methane was produced from the electrode reaction. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>