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Molecular Dissection of the Interaction between hBLT2 and the G Protein Alpha Subunits
Vukoti, Krishna Moorthy,Lee, Won-Kyu,Kim, Ho-Jun,Kim, Ick-Young,Yang, Eun-Gyeong,Lee, Cheol-Ju,Yu, Yeon-Gyu Korean Chemical Society 2007 Bulletin of the Korean Chemical Society Vol.28 No.6
Leukotriene B4 (LTB4) is a potent chemoattractant for leukocytes and considered to be an inflammatory mediator. Human BLT2 (hBLT2) is a low-affinity G-protein coupled receptor for LTB4 and mediates pertussis toxin-sensitive chemotactic cell movement. Here, we dissected the interaction between hBLT2 and G-protein alpha subunits using GST fusion proteins containing intracellular regions of hBLT2 and various Gα protein including Gα i1, Gα i2, Gα i3, Gα s1, Gα o1, and Gα z. Among the tested Gα subunits, Gα i3 showed the highest binding to the third intracellular loop region of hBLT2 with a dissociation constant (KD) of 5.0 × 10?6 M. These results suggest that Gα i3 has the highest affinity to hBLT2, and the third intracellular loop region of hBLT2 is the major component for the interaction with Gα i3.
Molecular Dissection of the Interaction between hBLT2 and the G Protein Alpha Subunits
Krishna Moorthy Vukoti,이원규,Ho-Jun Kim,김익영,Eun Gyeong Yang,이철주,유연규 대한화학회 2007 Bulletin of the Korean Chemical Society Vol.28 No.6
Leukotriene B4 (LTB4) is a potent chemoattractant for leukocytes and considered to be an inflammatory mediator. Human BLT2 (hBLT2) is a low-affinity G-protein coupled receptor for LTB4 and mediates pertussis toxin-sensitive chemotactic cell movement. Here, we dissected the interaction between hBLT2 and G-protein alpha subunits using GST fusion proteins containing intracellular regions of hBLT2 and various Ga protein including Ga i1, Ga i2, Ga i3, Ga s1, Ga o1, and Ga z. Among the tested Ga subunits, Ga i3 showed the highest binding to the third intracellular loop region of hBLT2 with a dissociation constant (KD) of 5.0 ´ 10-6 M. These results suggest that Ga i3 has the highest affinity to hBLT2, and the third intracellular loop region of hBLT2 is the major component for the interaction with Ga i3.