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      • An insight into bacterial population dynamics suing image analysis : a diurnal study in L. Soyang, Korea

        Choi, Seung-ik,Ahn Tae-seok,Kenje Kato 江原大學校 附設 環境硏究所 1999 環境硏究 Vol.16 No.-

        The development of computer-assisted image analysis has made it possible to measure the size of large numbers of bacterial particles. Using this approach, we elucidated diurnal changes in bacterial size distribution in L. Soyang, Korea. An apparent increase of badterial cell size was found during the daytime both in August and October for surface waters, where significant photosynthetic activity was observed. An increase in cell number was also confirmed. Thus, tight coupling between bacterial growth activity, cell size increase and photosynthetic activity was suggested In addition, a nocturnal increase in bacterial cell size was suggested in this lake, where bacterial doubling time was as short as several hours.

      • Effect of Starvation on Survival Rate and Body Composition of

        Yang-Su Kim*, Shinj i Yamamoto, Chihiro Aoki, Amal Biswas,Yoshifumi Sawada, Kenj i Takii and Osamu Murata 전남대학교 수산과학연구소 2014 수산과학연구소논문집 Vol.22 No.-

        The effect of starvation on Pacific bluefin tuna Thunnus orientalis (PBT) juveniles was investigated according to two stages, weaning stage (0.8±0.2 g) at 25 days post hatching (dph) and transportation stage (3.8±1.2 g) at 36 dph. The juveniles were then transferred (0 h) into each tank from the mass production tank. Starved PBT juveniles were collected from one random tank at 3, 6, 9, 29 and 93 h (25 dph), and 12, 24, 36, 48, 60 and 72 h (36 dph). The survival rate of 25 dph remained above 80% until 78 h: moreover the standard length and weight were increased until 9 h of starvation. However, the survival rate of 36 dph rapidly decreased to 42% at 48 h, and the standard length and weight were gradually decreased until the end of starvation. Whole body lipid and glycogen were rapidly consumed initially and moreover, the protein and energy contents were gradually and steadily decreased during starvation in both experiments. When compared to the linear regression analysis between energy consumption and starvation time, there was a statistical difference between the experiments (P < 0.05). These results suggest that energy mobilization differs between the weaning (25 dph) and transportation stages (36 dph). Further, it is assumed that the juveniles are more sensitive to starvation at 25 dph.

      • SCIESCOPUSKCI등재

        ORiginal Article : Relationships between Genetic Variations of PNPLA3, TM6SF2 and Histological Features of Nonalcoholic Fatty Liver Disease in Japan

        ( Norio Akuta ),( Yusuke Kawamura ),( Yasuji Arase ),( Fumitaka Suzuki ),( Hitomi Sezaki ),( Tetsuya Hosaka ),( Masahiro Kobayashi ),( Mariko Kobayashi ),( Satoshi Saitoh ),( Yoshiyuki Suzuki ),( Kenj 대한간학회 2016 Gut and Liver Vol.10 No.3

        Background/Aims: It is important to determine the noninvasive parameters of histological features in nonalcoholic fatty liver disease (NAFLD). The aim of this study was to investigate the value of genetic variations as surrogate markers of histological features. Methods: The parameters that affected the histological features of NAFLD were investigated in 211 Japanese patients with biopsy-proven NAFLD. The relationships between genetic variations in PNPLA3 rs738409 or TM6SF2 rs58542926 and histological features were analyzed. Furthermore, the impact of genetic variations that affected the pathological criteria for the diagnosis of nonalcoholic steatohepatitis (NASH) (Matteoni classification and NAFLD activity score) was evaluated. Results: The fibrosis stage of PNPLA3 GG was significantly more progressive than that of CG by multiple comparisons. Multivariate analysis identified PNPLA3 genotypes as predictors of fibrosis of stage 2 or more, but the impact tended to decrease at stage 3 or greater. There were no significant differences among the histological features of the three genotypes of TM6SF2. PNPLA3 genotypes partly affected the definition of NASH by the NAFLD activity score, but TM6SF2 genotypes did not affect the definition of NASH. Conclusions: In Japanese patients with biopsy-proven NAFLD, PNPLA3 genotypes may partly affect histological features, including stage of fibrosis, but the TM6SF2 genotype does not affect histological features. (Gut Liver 2016;10:437-445)

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