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Evaluation in Korean Soybean Cultivars of Resistance to Soybean Mosaic Virus
Yul-Ho Kim,Ok-Sun Kim,Jung-Kyeong Moon,Jae-Hwan Roh,Dae-Joon Im,Il-Bong Hur,Sang-Chul Lee 韓國作物學會 2001 Korean journal of crop science Vol.46 No.1
Soybean mosaic virus (SMV) resistance of Korean recommended soybeans was evaluated naturally and by mechanical inoculation in Suwon. Based on the differential reaction of forty-four soybean genotypes tested to nine different SMV strains, soybeans were classified into twenty-four groups. Myeongjunamulkong and Ilpumgeom-jeongkong showed a high degree of resistance to nine SMV strains, having no symptom. The other cultivars produced various reactions according to inoculation of each SMV strain: symptomless, mosaic or systemic necrosis. Only five cultivars such as Kwangankong, Eunhakong, Tawonkong, Namhaekong, Sobaegnamulkong were totally susceptible to every strain. There was variation in disease incidence. Soybeans, having the highest levels of resistance to G5H and G7H in the greenhouse, showed the lowest levels of SMV incidence in the field of Suwon. Myeong-junamulkong, Ilpumgeomjeongkong, Soyangkong, Pungsannamulkong, Sodamkong, Jangmikong, Geomjeong-kong2, Pureunkong, Sinpaldalkong2, Duyoukong, and Geumgangkong were fairly resistant to SMV. And SMV incidence of Taekwangkong, Saealkong and Baegunkong was over 45% with symptom of bud necrosis. And soybeans, highly resistant to SMV in the field and the greenhouse, were mainly derived from Jangyeobkong and Hwang-keumkong resistant to G1-G7.
Kim, Jung-Hwan,Lee, Chang-Kwon,Park, Hyo-Jun,Kim, Hyo-Jin,So, Hyun-Ha,Lee, Keun-Sang,Lee, Hwan-Myung,Roh, Hui-Yul,Choi, Wahn-Soo,Park, Tae-Kyu,Kim, Bo-Kyung Korean Physical Therapy Science 2006 대한물리치료과학회지 Vol.13 No.2
We investigated whether increased contractile responsiveness to epidermal growth factor (EGF) is associated with altered activation of mitogen-activated protein kinase (MAPK) in the aortic smooth muscle of deoxycorticosterone acetate (DOCA)-salt hypertensive rats. EGF induced contraction and MAPK activity in aortic smooth muscle strips, which were significantly increased in tissues from the DOCA-salt hypertensive rats compared with those from sham-operated rats. AG1478, PD98059, and LY294002, inhibitors of EGF receptor (EGFR) tyrosine kinase, MAPK/extracellular signal-regulated kinase (ERK) kinase, and phosphatidylinositol 3-kinase (PI3K), respectively, inhibited the contraction and the activity of ERK1/2 that were elevated by EGF. Y27632 and GF109203X, inhibitors of Rho kinase and protein kinase C, respectively, attenuated EGF-induced contraction, with no diminution of ERK1/2 activity. Although EGF also elevated the activity of EGFR tyrosine kinase in both sham-operated and DOCA-salt hypertensive rats, the expression and the magnitude of activation did not differ between strips. These results strongly suggest that EGF induces contraction by the activation of ERK1/2, which is regulated by the PI3K pathway in the aortic smooth muscle of DOCA-salt hypertensive rats.
Evaluation in Korean Soybean Cultivars of Resistance to Soybean Mosaic Virus
Kim, Yul-Ho,Kim, Ok-Sun,Moon, Jung-Kyeong,Roh, Jae-Hwan,Im, Dae-Joon,Hur, Il-Bong,Lee, Sang-Chul The Korean Society of Crop Science 2001 Korean journal of crop science Vol.46 No.1
Soybean mosaic virus (SMV) resistance of Korean recommended soybeans was evaluated naturally and by mechanical inoculation in Suwon. Based on the differential reaction of forty-four soybean genotypes tested to nine different SMV strains, soybeans were classified into twenty-four groups. Myeongjunamulkong and Ilpumgeom-jeongkong showed a high degree of resistance to nine SMV strains, having no symptom. The other cultivars produced various reactions according to inoculation of each SMV strain: symptomless, mosaic or systemic necrosis. Only five cultivars such as Kwangankong, Eunhakong, Tawonkong, Namhaekong, Sobaegnamulkong were totally susceptible to every strain. There was variation in disease incidence. Soybeans, having the highest levels of resistance to G5H and G7H in the greenhouse, showed the lowest levels of SMV incidence in the field of Suwon. Myeong-junamulkong, Ilpumgeomjeongkong, Soyangkong, Pungsannamulkong, Sodamkong, Jangmikong, Geomjeong-kong2, Pureunkong, Sinpaldalkong2, Duyoukong, and Geumgangkong were fairly resistant to SMV. And SMV incidence of Taekwangkong, Saealkong and Baegunkong was over 45% with symptom of bud necrosis. And soybeans, highly resistant to SMV in the field and the greenhouse, were mainly derived from Jangyeobkong and Hwang-keumkong resistant to G1-G7.
임신 중기 임부의 치주병 관련 위험요인과 일부 치주병 병원체의 분포
하정은 ( Jung Eun Ha ),여보미 ( Bo Mi Yeo ),노희열 ( Hui Yul Roh ),백대일 ( Dai Il Paik ),배광학 ( Kwang Hak Bae ) 대한예방치과·구강보건학회 2010 大韓口腔保健學會誌 Vol.34 No.4
Objectives. The objectives of this cross-sectional study were to examine the periodontal condition and health behavior of pregnant women and to analyze the distribution of periodontal pathogens by oral health behaviors. Methods. This study was designed as a hospital-based cross-sectional study. Pregnant women were recruited at about 24 weeks gestation from March 2009 to April 2010 at the obstetrics clinic of general hospital located in Seoul, South Korea. The information of demographic, behavior and health conditions that may influence periodontitis were collected. Full mouth periodontal probing was taken by one trained examiner for the diagnosis of periodontitis. The periodontitis was defined as periodontal attachment loss of 3.5 mm and over on 2 or more sites not on same tooth. Subgingival biofilm and gingival crevicular fluid were collected using two sterilized paper point (#20) for quantitative analysis of prevotella intermedia, porphyromonas gingivalis and treponema denticola. Results. One hundred thirteen pregnant women were included. Adjusted odds ratio of use the floss or interdental brush was 0.22. Pregnant women using the floss or interdental brush, and receiving the scaling before pregnancy had the lower level of T. denticola, P. gingivalis and P. intermedia than pregnant women who didn`t use the floss or interdental brush and not receive the scaling. Conclusions. Oral health behaviors, such as use the floss or interdental brush, may be enable to promote the women`s oral health and to prevent the preterm birth.
Biomedical Engineering Research on Circulatory Disorders
Yoo Jung-Yul,Park, Jae-Hyung,Suh Sang-Ho,Shim Eun-Bo,Rhee Kye-Han,Shin, Se-Hyun,Cho, Young-I.,Kim, C. Sean,Roh, Hyung-Woon Biomedical Engineering Society for Circulation 2004 International Journal of Vascular Biomedical Engin Vol.2 No.1
Circulatory disease is the number two cause of death next to cancer in Korea, while the cardiovascular disease alone is the number one cause of death in the US. In the present article, some background, current status and future prospects of biomedical engineering esearch on circulatory disorders are discussed in terms of the origin of atherosclerosis, computational fluid dynamics and medical imaging techniques, clinical treatments and fluid dynamics, advances in stents, hemodynamic analysis of artificial heart, and artificial blood. In particular, the importance of close collaboration of medicine and fluids engineering is emphasized.
Kyu-Seok Jung,Seung-Mi Seo,Hye-Jin Jeon,Bo-Reum Jeong,Eun-Jung Roh,Jae-Gee Ryu,Kyoung-Yul Ryu 한국토양비료학회 2018 한국토양비료학회지 Vol.51 No.4
Addition of animal manure to soil can provide opportunity for bacterial pathogens contamination of soil, water, and food. This study was conducted to investigate the survival of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in soil and compost amended soil under the selected environmental conditions. Soil and compost amended soil were inoculated with S. enterica, E. coli O157:H7, and L. monocytogenes. Soil and compost amended soil were incubated at 25°C and consistent moisture content. Samples had been collected during 200 days depending on the given conditions. S. enterica and E. coli O157:H7 survived over 200 days in soil and compost amended soil. L. monocytogenes persisted for 80 days in soil and for 160 days in compost amended soil, respectively. S. enterica and E. coli O157:H7 survived longer than L. monocytogenes at soil and compost amended soil. It is noted that S. enterica, E. coli O157:H7, and L. monocytogenes survived long in soil and compost amended soil. S. enterica, E. coli O157:H7, and L. monocytogenes survived longer in compost amended soil than in soil. Results from these studies provide useful information in identifying manure handling practices to reduce the risk of S. enterica, E. coli O157:H7, and L. monocytogenes transmission to foods produced in the presence of animal waste.
정지은(Jieun Jung),서승미(Seung-Mi Seo),양수인(SuIn Yang),진현숙(Hyeon-Suk Jin),정규석(Kyu-Seok Jung),노은정(Eunjung Roh),정명인(Myeong-In Jeong),류재기(Jae-Gee Ryu),류경열(Kyoung-Yul Ryu),오광교(Kwang Kyo Oh) 한국식품과학회 2021 한국식품과학회지 Vol.53 No.6
본 연구는 고추와 토양, 농업용수, 장갑을 대상으로 101개의 시료를 채취하여 위생지표세균(일반세균, 대장균군, 대장균)과 B. cereus, S. aureus에 대해 정량 분석과 병원성 세균(병원성 E. coli, E. coli O157:H7, B. cereus, S. aureus, L. monocytogenes, Salmonella spp.)에 대해 정성 분석하였다. 고추와 토양, 장갑 시료의 일반세균수는 3.36-7.08 log CFU/g, 대장균군은 2.16-5.14 log CFU/g, 대장균은 1.50-1.54 log CFU/g이었고 농업용수 시료의 대장균군은 17.67-247.05 MPN/100 mL 수준으로 나타났다. 고추와 토양, 농업용수, 장갑의 B. cereus는 1.35-6.78 log CFU/g, S. aureus는 1.63-2.09 log CFU/g 수준으로 나타났다. 고추와 토양, 농업용수, 장갑 시료에서 병원성 E. coli, E. coli O157:H7, L. monocytogenes, Salmonella spp. 등은 검출되지 않았다. 고추와 환경 시료에서 분리한 B. cereus의 장독소 유전자와 항생제 감수성을 분석하였다. 고추와 토양, 장갑에서 분리한 B. cereus의 hblACD 유전자는 각각 54.5, 75, 75% 비율이었으며, nheABC유전자는 각각 72.7, 75, 100% 비율이었다. 고추와 토양, 장갑에서 분리한 B. cereus는 β-lactam계 항생제에 대해 저항을 보였지만, cefotaxime에 대해 일부 균주는 중간 내성을 보였고 모든 균주는 imipenem에 대해 감수성을 나타냈다. B. cereus는 비β-lactam계 항생제에 대해 감수성을 나타냈지만, rifampin, trimethoprim-sulfamethoxaole, vancomycin, clindamycin, erythromycin에 대해 일부 균주는 중간 내성을 나타냈다. 본 연구의 결과를 통하여 재배단계 고추의 미생물학적 오염도를 파악할 수 있고, 고추에 오염된 B. cereus에 의해 설사형 식중독이 발생 가능성을 파악할 수 있다. 이는 농산물 중 미생물 기준 설정 등에 대한 과학적 근거로써 활용이 가능할 것으로 기대한다. 또한, 고추와 재배환경에서 항생제 저항성 B. cereus가 검출되어 농업현장에서 항생제 내성균주 출현을 예방하는 대책이 요구된다. This study was performed to investigate the extent of microbial contamination, the presence of enterotoxin genes, and the antibiotic susceptibility of Bacillus cereus in 58 red pepper plants and 43 environmental samples (soil, irrigation water, and gloves) associated with the plant cultivation. The detected counts of total aerobic bacteria, coliform bacteria, Escherichia coli, Bacillus cereus, and Staphylococcus aureus were lower in these samples, as compared to the regulations of standards for foods; moreover, pathogens, such as E. coli, E. coli O157:H7, Listeria monocytogenes, and Salmonella spp., were not detected. Genes encoding hemolysin BL enterotoxins (hblA, hblC, and hblD) as well as non-hemolytic enterotoxins (nheA, nheB, and nheC) were detected in 23 B. cereus specimens that were isolated from the test samples and had β-hemolytic activity. Interestingly, B. cereus is resistant to β-lactam and susceptible to non-β-lactam antibiotics. However, in this case, the isolated B. cereus specimens exhibited a shift from resistant to intermediate in response to cefotaxime and from susceptible to intermediate in case of rifampin, trimethoprim-sulfamethoxazole, vancomycin, clindamycin, and erythromycin. Therefore, the levels of B. cereus should be monitored to detect changes in antibiotic susceptibility and guarantee their safety.