http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Jang, Jiwon,Byun, Sung-Hyun,Han, Dasol,Lee, Junsub,Kim, Juwan,Lee, Nayeon,Kim, Inhee,Park, Soojeong,Ha, Soobong,Kwon, Mookwang,Ahn, Jyhyun,Chung, Woo-Jae,Kweon, Dae-Hyuk,Cho, Jae Youl,Kim, Sunyoung,Yo Mary Ann Liebert 2014 STEM CELLS AND DEVELOPMENT Vol.23 No.23
<P>Notch has a broad range of regulatory functions in many developmental processes, including hematopoiesis, neurogenesis, and angiogenesis. Notch has several key functional regions such as the RBP-Jκ/CBF1 association module (RAM) domain, nuclear localization signals (NLS), and ankyrin (ANK) repeats. However, previous reports assessing the level of importance of these domains in the Notch signaling pathway are controversial. In this study, we have assessed the level of contribution of each Notch domain to the regulation of mammalian neural stem cells in vivo as well as in vitro. Reporter assays and real-time polymerase chain reactions show that the ANK repeats and RAM domain are indispensable to the transactivation of Notch target genes, whereas a nuclear export signal (NES)-fused Notch intracellular domain (NICD) mutant defective in nuclear localization exerts a level of activity comparable to unmodified NICD. Transactivational ability appears to be tightly coupled to Notch functions during brain development. Unlike ANK repeats and RAM domain deletion mutants, NES-NICD recapitulates NICD features such as promotion of astrogenesis at the expense of neurogenesis in vitro and enhancement of neural stem cell character in vivo. Our data support the previous observation that intranuclear localization is not essential to the oncogenesis of Notch1 in certain types of cells and imply the importance of the noncanonical Notch signaling pathway in the regulation of mammalian neural stem cells.</P>
Jang, Jiwon,Wang, Yidi,Kim, Hyung-Seok,Lalli, Matthew A.,Kosik, Kenneth S. Wiley (John WileySons) 2014 Stem Cells Vol.32 No.10
<P>Nuclear factor, erythroid 2-like 2 (Nrf2) is a master transcription factor for cellular defense against endogenous and exogenous stresses by regulating expression of many antioxidant and detoxification genes. Here, we show that Nrf2 acts as a key pluripotency gene and a regulator of proteasome activity in human embryonic stem cells (hESCs). Nrf2 expression is highly enriched in hESCs and dramatically decreases upon differentiation. Nrf2 inhibition impairs both the self-renewal ability of hESCs and re-establishment of pluripotency during cellular reprogramming. Nrf2 activation can delay differentiation. During early hESC differentiation, Nrf2 closely colocalizes with OCT4 and NANOG. As an underlying mechanism, our data show that Nrf2 regulates proteasome activity in hESCs partially through proteasome maturation protein (POMP), a proteasome chaperone, which in turn controls the proliferation of self-renewing hESCs, three germ layer differentiation and cellular reprogramming. Even modest proteasome inhibition skews the balance of early differentiation toward mesendoderm at the expense of an ectodermal fate by decreasing the protein level of cyclin D1 and delaying the degradation of OCT4 and NANOG proteins. Taken together, our findings suggest a new potential link between environmental stress and stemness with Nrf2 and the proteasome coordinately positioned as key mediators.</P>
발산제약 이동최소자승법 기반 벡터장을 생성하기 위한 효율적인 학습 표현
장지원(Jiwon Jang),이수빈(Subin Lee),김종현(Jong-Hyun Kim) 한국컴퓨터정보학회 2024 한국컴퓨터정보학회 학술발표논문집 Vol.32 No.1
본 논문에서는 다항식 보간법의 일종인 이동최소자승법(Moving least squares, MLS)을 네트워크로 학습하여, Divergence-constrained MLS 벡터장을 효율적으로 표현하는 방법을 제안한다. 벡터장을 구성하기 위해 MLS는 스칼라가 아닌 벡터 보간을 해야 하므로 행렬과 벡터의 크기가 더 커지며, 이는 계산량이 커짐을 나타낸다. 고차 보간(High-order interpolation)이 가능한 특징은 장점이 되지만, 계산량이 매우 크기 때문에 시뮬레이션에는 활용이 어렵다. Divergence-constrained MLS를 유체 시뮬레이션에 적용한 경우가 있지만, 실제로 슈퍼컴퓨터(Supercomputer)를 해야 장면 제작이 가능하므로 효용성이 떨어진다. 본 논문에서는 이러한 문제를 해결하기 위해 네트워크 학습을 통한 Divergence-constrained MLS 벡터장을 표현할 수 있는 결과를 보여준다.