2002년 기부횟수 자료의 재분석: 수정 및 보완

김병수,이주형,김인영,박수범,박태규,Kim, Byung Soo,Lee, Juhyung,Kim, Inyoung,Park, Su-Bum,Park, Tae-Kyu 한국통계학회 2014 응용통계연구 Vol.27 No.5

Kim 등 (2006)과 Kim 등 (2009)은 2002년에 (사)볼런티어 21에서 조사한 설문자료에 기초하여 우리나라 개인의 기부횟수에 영향을 주는 유의적 설명변수를 보고한 바 있다. 본고에서는 Kim 등 (2006)과 Kim 등 (2009)의 계산오류를 발견하여 이를 수정하고, 아울러 Kim 등 (2009)이 적용한 0이 팽창된 포아송 모형에 로지스틱 회귀모형을 추가하였다. 동 로지스틱 모형으로 기부행위(0, 1)에 영향을 주는 설명변수를 식별하고, 아울러 기부횟수가 작은 군(群)과 큰 군(群)을 판별하여 주는 설명변수를 식별하고자 한다. Kim et al. (2006) and Kim et al. (2009) reported a set of explanatory variables affecting donation frequency when they analyzed nationwide survey data on donations collected in 2002 by Volunteer 21, a nonprofit organization in Korea. The primary purpose of this paper is to correct computational errors found in Kim et al. (2006) and Kim et al. (2009), to rectify major results in the Tables and Figures and to supplement Kim et al. (2009) by providing new results. We add two logistic regressions to the ZIP and a mixture of two Poisson regressions of Kim et al. (2009). Through these two logistic regressions we could detect a set of explanatory variables affecting donation activity (0 or 1) and another set of explanatory variables, in which the volunteer (0, 1) variable is common, discriminating the infrequent donor group from the frequent donor group.

[PA-0007] Transcription factors regulated by LEAFY COTYLEDON 2 in triacylglycerol accumulation

Inyoung Kim(Inyoung Kim),Hyung Ju Do(Hyung Ju Do),Hyun Uk Kim(Hyun Uk Kim) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Kruppel-like factor 4 attenuates osteoblast formation, function, and cross talk with osteoclasts

Kim, Jung Ha,Kim, Kabsun,Youn, Bang Ung,Lee, Jongwon,Kim, Inyoung,Shin, Hong-In,Akiyama, Haruhiko,Choi, Yongwon,Kim, Nacksung The Rockefeller University Press 2014 The Journal of cell biology Vol.204 No.6

<P>KLF4 controls bone homeostasis by negatively regulating both osteoclast and osteoblast differentiation.</P>

BCAP promotes osteoclast differentiation through regulation of the p38-dependent CREB signaling pathway

Kim, Jung Ha,Kim, Kabsun,Kim, Inyoung,Seong, Semun,Lee, Keun-Bae,Kim, Nacksung Elsevier 2018 Bone Vol.107 No.-

<P><B>Abstract</B></P> <P>Many studies have determined that PI3K-Akt signaling pathways play important roles in osteoclast differentiation and function. In the present study, we investigated the roles of B-cell adaptor for PI3K (BCAP), which is a PI3K binding molecule, in osteoclasts. Overexpression of BCAP in osteoclast precursor cells enhanced osteoclast differentiation induced by tumor necrosis factor alpha (TNF-α) as well as receptor activator of nuclear factor-κB ligand (RANKL). Conversely, osteoclast differentiation mediated by both cytokines was attenuated when BCAP expression was downregulated using small interfering RNA. Notably, BCAP induced Akt activation only upon stimulation by RANKL, but not by TNF-α. However, BCAP activated p38-dependent cAMP response element-binding protein (CREB) phosphorylation induced by both RANKL and TNF-α. Collectively, we showed that BCAP plays an important role in osteoclast differentiation by regulating the p38-dependent CREB signaling pathway, and that BCAP might be a new therapeutic target for bone diseases.</P> <P><B>Highlights</B></P> <P> <UL> <LI> BCAP positively regulates both RANKL- and TNF-α-induced osteoclast differentiation. </LI> <LI> BCAP increases the phosphorylation of Akt by RANKL, but not TNF-α. </LI> <LI> BCAP enhances both RANKL- and TNF-α-induced CREB activation. </LI> <LI> BCAP promotes osteoclast differentiation by regulating p38-dependent CREB signaling pathway rather than PI3K-Akt signaling pathway. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

NRROS Negatively Regulates Osteoclast Differentiation by Inhibiting RANKL-Mediated NF-κB and Reactive Oxygen Species Pathways

Kim, Jung Ha,Kim, Kabsun,Kim, Inyoung,Seong, Semun,Kim, Nacksung Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.10

Negative regulator of reactive oxygen species (NRROS) is known to repress ROS generation in phagocytes. In this study, we examined the roles of NRROS in both osteoclasts and osteoblasts. Our results demonstrate that NRROS negatively regulates the differentiation of osteoclasts, but not osteoblasts. Further, overexpression of NRROS in osteoclast precursor cells attenuates RANKL-induced osteoclast differentiation. Conversely, osteoclast differentiation is enhanced upon siRNA-mediated knock-down of NRROS. Additionally, NRROS attenuates RANKL-induced $NF-{\kappa}B$ activation, as well as degradation of the NOX1 and NOX2 proteins, which are required for ROS generation. Based on our observations, we present NRROS as a novel negative regulator of RANKL-induced osteoclastogenesis.

Effect of centrifugation on tryptic protein digestion

Kim, Soohwan,Kim, Yeoseon,Lee, Dabin,Kim, Inyoung,Paek, Jihyun,Shin, Dongwon,Kim, Jeongkwon The Korean Society of Analytical Science 2017 분석과학 Vol.30 No.2

This study investigated the effect of centrifugation on tryptic digestion. This was done by applying different centrifugation speeds (6,000, 8,000, 10,000, 20,000, and $30,000{\times}g$) over various durations (0, 10, 20, 30, 40, 50, and 60 min) to digest two model proteins - cytochrome c and myoglobin. The intact proteins and resulting peptides were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Centrifugation greatly improved the tryptic digestion efficiency of cytochrome c, where either an increase in centrifugation speed or in digestion duration significantly improved the digestion of cytochrome c. However, centrifugation did not noticeably improve the digestion of myoglobin; 16 h of centrifuge-assisted tryptic digestion at $30,000{\times}g$ barely removed the myoglobin protein peak. Similar results were also obtained when using conventional tryptic digestion with gentle mixing. When acetonitrile (ACN) was added to make 10% ACN buffer solutions, the myoglobin protein peak disappeared after 6 h of digestion using both centrifuge-assisted and conventional tryptic digestions.

Unenhanced Breast MRI With Diffusion-Weighted Imaging for Breast Cancer Detection: Effects of Training on Performance and Agreement of Subspecialty Radiologists

Kim Yeon Soo,Lee Su Hyun,Kim Soo-Yeon,Kim Eun Sil,Park Ah Reum,Chang Jung Min,Park Vivian Youngjean,Yoon Jung Hyun,Kang Bong Joo,Yun Bo La,Kim Tae Hee,Ko Eun Sook,Chu A Jung,Kim Jin You,Youn Inyoung,C 대한영상의학회 2024 Korean Journal of Radiology Vol.25 No.1

Objective: To investigate whether reader training improves the performance and agreement of radiologists in interpreting unenhanced breast magnetic resonance imaging (MRI) scans using diffusion-weighted imaging (DWI). Materials and Methods: A study of 96 breasts (35 cancers, 24 benign, and 37 negative) in 48 asymptomatic women was performed between June 2019 and October 2020. High-resolution DWI with b-values of 0, 800, and 1200 sec/mm2 was performed using a 3.0-T system. Sixteen breast radiologists independently reviewed the DWI, apparent diffusion coefficient maps, and T1-weighted MRI scans and recorded the Breast Imaging Reporting and Data System (BI-RADS) category for each breast. After a 2-h training session and a 5-month washout period, they re-evaluated the BI-RADS categories. A BI-RADS category of 4 (lesions with at least two suspicious criteria) or 5 (more than two suspicious criteria) was considered positive. The per-breast diagnostic performance of each reader was compared between the first and second reviews. Inter-reader agreement was evaluated using a multi-rater κ analysis and intraclass correlation coefficient (ICC). Results: Before training, the mean sensitivity, specificity, and accuracy of the 16 readers were 70.7% (95% confidence interval [CI]: 59.4–79.9), 90.8% (95% CI: 85.6–94.2), and 83.5% (95% CI: 78.6–87.4), respectively. After training, significant improvements in specificity (95.2%; 95% CI: 90.8–97.5; P = 0.001) and accuracy (85.9%; 95% CI: 80.9–89.8; P = 0.01) were observed, but no difference in sensitivity (69.8%; 95% CI: 58.1–79.4; P = 0.58) was observed. Regarding inter-diffusionreader agreement, the κ values were 0.57 (95% CI: 0.52–0.63) before training and 0.68 (95% CI: 0.62–0.74) after training, with a difference of 0.11 (95% CI: 0.02–0.18; P = 0.01). The ICC was 0.73 (95% CI: 0.69–0.74) before training and 0.79 (95% CI: 0.76–0.80) after training (P = 0.002). Conclusion: Brief reader training improved the performance and agreement of interpretations by breast radiologists using unenhanced MRI with DWI.

A simple and robust route toward flexible CIGS photovoltaic devices on polymer substrates: Atomic level microstructural analysis and local opto-electronic investigation

Kim, Kihwan,Kim, Juran,Gang, Myeng Gil,Kim, Se-Ho,Song, Soomin,Cho, Yunae,Shin, Donghyeop,Eo, Young-Joo,Jeong, Inyoung,Ahn, Seung Kyu,Cho, Ara,Kim, Jayeong,Yoon, Seokhyun,Choi, Pyuck-Pa,Jo, William,Ki Elsevier 2019 Solar energy materials and solar cells Vol.195 No.-

<P><B>Abstract</B></P> <P>In this work, copper indium gallium selenide (Cu(In,Ga)Se<SUB>2</SUB>; CIGS) absorbers were grown on polyimide (PI)/molybdenum substrates by a three-stage co-evaporation process at various temperatures, film formation was systemically studied using various advanced characterization methods such as transmission electron microscopy, micro-Raman spectroscopy, Kelvin probe force microscopy, and atom probe tomography. The CIGS films on PI were found to exhibit considerable physical and electrical variations with respect to the process temperature of three-stage co-evaporation. In particular, when the process temperature reached 400 °C, the CIGS absorber on PI began to exhibit controlled microstructure and intergrain properties. By adjusting the microstructure and intergrain properties of the absorber films by means of the process temperature of three-stage co-evaporation, flexible CIGS solar cells on PI with an efficiency of 16.7% (with anti-refection coating) were achieved.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CIGS absorber films were grown on flexible polyimide/molybdenum substrates. </LI> <LI> Low-temperature three-stage process (≤440 °C) with extrinsic Na addition was used to CIGS growth. </LI> <LI> CIGS film evolution was systemically observed using advanced material characterization techniques. </LI> <LI> Highly efficient CIGS cells on flexible polyimide substrates were realized while maintaining process manufacturability. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Endoplasmic Reticulum–Bound Transcription Factor CREBH Stimulates RANKL-Induced Osteoclastogenesis

Kim, Jung Ha,Kim, Kabsun,Kim, Inyoung,Seong, Semun,Nam, Kwang-Il,Kim, Kyung Keun,Kim, Nacksung American Association of Immunologists 2018 Journal of Immunology Vol. No.

<P>Endoplasmic reticulum (ER) stress is triggered by various metabolic factors, such as cholesterol and proinflammatory cytokines. Recent studies have revealed that ER stress is closely related to skeletal disorders, such as osteoporosis. However, the precise mechanism by which ER stress regulates osteoclast differentiation has not been elucidated. In this study, we identified an ER-bound transcription factor, cAMP response element-binding protein H (CREBH), as a downstream effector of ER stress during RANKL-induced osteoclast differentiation. RANKL induced mild ER stress and the simultaneous accumulation of active nuclear CREBH (CREBH-N) in the nucleus during osteoclastogenesis. Overexpression of CREBH-N in osteoclast precursors enhanced RANKL-induced osteoclast formation through NFATc1 upregulation. Inhibiting ER stress using a specific inhibitor attenuated the expression of osteoclast-related genes and CREBH activation. In addition, inhibition of reactive oxygen species using <I>N</I>-acetylcysteine attenuated ER stress, expression of osteoclast-specific marker genes, and RANKL-induced CREBH activation. Furthermore, inhibition of ER stress and CREBH signaling pathways using an ER stress–specific inhibitor or CREBH small interfering RNAs prevented RANKL-induced bone destruction in vivo. Taken together, our results suggest that reactive oxygen species/ER stress signaling-dependent CREBH activation plays an important role in RANKL-induced osteoclastogenesis. Therefore, inactivation of ER stress and CREBH signaling pathways may represent a new treatment strategy for osteoporosis.</P>

IRF2 enhances RANKL-induced osteoclast differentiation via regulating NF-κB/NFATc1 signaling

( Inyoung Kim ),( Jung Ha Kim ),( Kabsun Kim ),( Semun Seong ),( Keun-bae Lee ),( Nacksung Kim ) 생화학분자생물학회 2021 BMB Reports Vol.54 No.9

Interferon regulatory factors (IRFs) play roles in various biological processes including cytokine signaling, cell growth regulation and hematopoietic development. Although it has been reported that several IRFs are involved in bone metabolism, the role of IRF2 in bone cells has not been elucidated. Here, we investigated the involvement of IRF2 in RANKL-induced osteoclast differentiation. IRF2 overexpression in osteoclast precursor cells enhanced osteoclast differentiation by regulating the expression of NFATc1, a master regulator of osteoclastogenesis. Conversely, IRF2 knockdown inhibited osteoclast differentiation and decreased the NFATc1 expression. Moreover, IRF2 increased the translocation of NF-κB subunit p65 to the nucleus in response to RANKL and subsequently induced the expression of NFATc1. IRF2 plays an important role in RANKL-induced osteoclast differentiation by regulating NF-κB/NFATc1 signaling pathway. Taken together, we demonstrated the molecular mechanism of IRF2 in osteoclast differentiation, and provide a molecular basis for potential therapeutic targets for the treatment of bone diseases characterized by excessive bone resorption. [BMB Reports 2021; 54(9): 482-487]