진균 세포벽 형성 저해물질 생성 Streptomyces속 세균의 분리 및 수리동정

서원나,박정희,이지영,김인섭,이계준,배무 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

본 연구의 목적은 진균의 세포벽 합성을 저해하는 항진균 물질생성 방선균을 개발하는 데 있다. 다양한 환경으로부터 채취한 토양 시료로부터 방선균 선택 배지를 이용하여 860여 방선균을 선택적으로 분리하였다. 분리 균주 중 Penicillium cyclopium, Cryptococcus laurentii, Aspergillus flavus에 특이적 항균 활성 물질을 생산하는 균주 EMS4, EMP22, L234를 각각 선별하였다. 화학적 동정과 형태학적 동정을 통해 분리 균주들이 Streptomyces속 세균임을 알 수 있었다. 총 50개의 주군집, 34개의 부군집 형질을 분석하고, 이 실험 결과를 TAXON program에 적용하여 종의 수리동정을 실시하였다. 그 결과 분리균 EMS4는 Streptomyces의 제 61 주군집에 속하는 Streptomyces lavendulae의 한 균주로 동정되었고, EMP22는 제 1B 주군집에 속하는 S. willmorei의 한 균주로 동정되었으며, L234는 제 3 부군집의 S. aburaviensis의 한 균주로 동정되었다. The aim of the present research program wa to identify and develop stains of actinomycetes producing antifungal antibiotics which inhibit cell wall biosynthesis. 860 strains of Actionomycetes were isolated from various soil samples. Three isolates, EMS4, EMP22, and L234 were selected as the strains producing antifungal antibiotics inducing abnormal morphology against Penicillium cyclopium. Cryptococcus laurentii, and Aspergillus flavus, respectively. Taxonomic unit characters of the strains were tested and the data were analyzed numerically using TAXON program. EMS4, EMP22, and L234 were identified to be a member of Streptomyces lavendulae, Streptomyces willmorei, and Streptomyces aburaviensis, respectively.

진균 세포벽 형성 저해물질 생성 Streptomyces속 세균의 분리 및 수리동정

서원나,박정희,이지영,김인섭,이계준,배무 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-

본 연구의 목적은 진균의 세포벽 합성을 저해하는 항진균 물질생성 방선균을 개발하는 데 있다. 다양한 환경으로부터 재취한 토양 시료로부터 방선균 선택 배지를 이용하여 860여 방선균을 선택적으로 분리하였다. 분리 균주 중 Penicillum cyclopium, Cryptococcus laurentii, Aspergillus flavus에 특이적 항균 활성 물질을 생산하는 균주 EMS4, EMP22, L234를 각각 선별하였다. 화학적 동정과 형태학적 동정을 통해 분리 균주들이 Streptomyces속 세균임을 알 수 있었다. 총 50개의 주군집, 34개의 부군집 형질을 분석하고, 이 실험 결과를 TAXON program에 적용하여 종의 수리동정을 실시하였다. 그 결과 분리균 EMS4는 Streptomyces의 제 61 주군집에 속하는 Streptomyces lavendulae의 한 균주로 동정되었고, EMP22는 제 1B 주군집에 속하는 S. willmorei의 한 균주로 동정되었으며, L234는 제 3 부군집의 S. aburaviensis의 한 균주로 동정되었다. The aim of the present research program was to identify and develop strains of actinomycetes producing antifungal antibiotics which inhibit cell wall biosynthesis. 860 strains of Actinomycetes were isolated from various soil samples. Three isolates, EMS4, EMP22, and L234 were selected as the strains producing antifungal antibiotics inducing abnormal morphology against Penicillium cyclopium, Cryptococcus laurentii, and Aspergillus flavus, respectively. Taxonomic unit characters of the strains were tested and the data were analyzed numerically using TAXON program. EMS 4,EMP22, and L234 were identified to be a member of Streptomyces lavendulae, Streptomyces willmorei, and Streptomyces aburaviensis, respectively.

Zingiber mioga (Thunb.) Roscoe attenuates allergic asthma induced by ovalbumin challenge

SHIN, NA-RAE,SHIN, IN-SIK,JEON, CHAN-MI,HONG, JU-MI,KWON, OK-KYOUNG,KIM, HUI-SEONG,OH, SEI-RYANG,HAHN, KYU-WOUNG,AHN, KYUNG-SEOP SPANDIDOS PUBLICATIONS 2015 MOLECULAR MEDICINE REPORTS Vol.12 No.3

<P>Zingiber mioga (Thunb.) Roscoe (ZM) is a traditional medicine, used to treat inflammatory diseases. The present study aimed to evaluate the inhibitory effects of ZM on the inflammatory response in lipopolysaccharide (LPS)?stimulated RAW264.7 murine macrophage cells and in a mouse model of ovalbumin (OVA)?induced allergic asthma. Mice received OVA sensitization on day 0 and 14, and were challenged with OVA between days 21 and 23. ZM was administered to the mice at a dose of 30 mg/kg, 1 h prior to OVA challenge. In LPS?stimulated RAW264.7 cells, ZM significantly decreased nitric oxide (NO) and tumor necrosis factor (TNF)?α production in a concentration?dependent manner, and mRNA expression of inducible NO synthase (iNOS), TNF?α and matrix metalloproteinase (MMP)?9 was reduced. In addition, treatment with ZM decreased the inflammatory cell count in bronchoalveolar lavage fluid from the mice, and reduced the expression of interleukin (IL)?4, IL?5, IL?13, eotaxin and immunoglobulin E. ZM also reduced airway hyperresponsiveness in OVA?challenged mice, and attenuated the infiltration of inflammatory cells and mucus production in the airways, with a decrease in the expression of iNOS and MMP?9 in lung tissue. In conclusion, the results of the present study indicate that ZM effectively inhibits inflammatory responses. Therefore, it may be that ZM has potential as a therapeutic agent for use in inflammatory diseases.</P>

Thuja orientalis reduces airway inflammation in ovalbumin-induced allergic asthma

SHIN, IN-SIK,SHIN, NA-RAE,JEON, CHAN-MI,KWON, OK-KYOUNG,HONG, JU-MI,KIM, HUI-SEONG,OH, SEI-RYANG,AHN, KYUNG-SEOP SPANDIDOS PUBLICATIONS 2015 MOLECULAR MEDICINE REPORTS Vol.12 No.3

<P>Thuja orientalis (TO) may be used as a herbal remedy for the treatment of numerous inflammatory diseases. In the present study, the effects of TO were evaluated on airway inflammation in ovalbumin (OVA)?induced allergic asthma and RAW264.7 murine macrophage cells. The effects of TO on the production of proinflammatory mediators, were determined in RAW264.7 cells that had been stimulated with lipopolysaccharide (LPS). Furthermore, an in vivo experiment was performed on mice that were sensitized to OVA and then received an OVA airway challenge. TO was administered by daily oral gavage at a dose of 30 mg/kg, 21?23 days after the initial OVA sensitization. TO was shown to reduce nitric oxide production and reduce the relative mRNA expression levels of inducible nitric oxide synthase (iNOS), interleukin (IL)?6, cyclooxygenase?2, matrix metalloproteinase (MMP)?9, and tumor necrosis factor?α in RAW264.7 cells stimulated with LPS. In addition, TO markedly decreased the inflammatory cell counts in bronchial alveolar lavage fluid, reduced the levels of IL?4, IL?5, IL?13, eotaxin and immunoglobulin E, and reduced airway hyperresponsivenes, in the OVA sensitized mice. Furthermore, TO attenuated airway inflammation and mucus hypersecretion, induced by the OVA challenge of the lung tissue. TO also reduced the expression of iNOS and MMP?9 in lung tissue. In conclusion, TO exerted anti?inflammatory effects in an OVA?induced allergic asthma model, and in LPS?stimulated RAW264.7 cells. These results suggest that TO may be a useful therapeutic agent for the treatment of inflammatory diseases, including allergic asthma.</P>

Clausena anisata-mediated protection against lipopolysaccharide-induced acute lung injury in mice

JEON, CHAN-MI,SHIN, IN-SIK,SHIN, NA-RAE,HONG, JU-MI,KWON, OK-KYOUNG,KIM, JUNG-HEE,OH, SEI-RYANG,BACH, TRAN-THE,HAI, DO-VAN,QUANG, BUI-HONG,CHOI, SANG-HO,LEE, JOONGKU,MYUNG, PYUNG-KEUN,AHN, KYUNG-SEOP Spandidos Publications 2016 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.37 No.4

<P>Clausena anisata (Willd.) Hook.f. ex Benth. (CA), which is widely used in traditional medicine, reportedly exerts antitumor, anti-inflammatory and other important therapeutic effects. The aim of the present study was to investigate the potential therapeutic effects of CA in a mouse model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) and in LPS-stimulated RAW 264.7 cells. Male C57BL/6 mice were administered treatments for 3 days by oral gavage. On day 3, the mice were instilled intranasally with LPS or PBS followed 3 h later by oral CA (30 mg/kg) or vehicle administration. In vitro, CA decreased nitric oxide (NO) production and pro-inflammatory cytokines, such as interleukin (IL)-6 and prostaglandin E2 (PGE(2)), in LPS-stimulated RAW 264.7 cells. CA also reduced the expression of pro-inflammatory mediators, such as cyclooxygenase-2. In vivo, CA administration significantly reduced inflammatory cell numbers in the bronchoalveolar lavage fluid (BALF) and suppressed pro-inflammatory cytokine levels, including tumor necrosis factor-alpha (TNF-alpha), IL-6, and IL-1 beta, as well as reactive oxygen species production in the BALF. CA also effectively reduced airway inflammation in mouse lung tissue of an LPS-induced ALI mouse model, in addition to decreasing inhibitor kappa B (I kappa B) and nuclear factor-kappa B (NF-kappa B) p65 phosphorylation. Taken together, the findings demonstrated that CA inhibited inflammatory responses in a mouse model of LPS-induced ALI and in LPS-stimulated RAW 264.7 cells. Thus, CA is a potential candidate for development as an adjunctive treatment for inflammatory disorders, such as ALI.</P>

Copper oxide nanoparticles aggravate airway inflammation and mucus production in asthmatic mice via MAPK signaling

Park, Ji-Won,Lee, In-Chul,Shin, Na-Rae,Jeon, Chan-Mi,Kwon, Ok-Kyoung,Ko, Je-Won,Kim, Jong-Choon,Oh, Sei-Ryang,Shin, In-Sik,Ahn, Kyung-Seop Informa UK (Informa Healthcare) 2016 Nanotoxicology Vol.10 No.4

<P>Copper oxide nanoparticles (CuONPs), metal oxide nanoparticles were used in multiple applications including wood preservation, antimicrobial textiles, catalysts for carbon monoxide oxidation and heat transfer fluid in machines. We investigated the effects of CuONPs on the respiratory system in Balb/c mice. In addition, to investigate the effects of CuONPs on asthma development, we used a murine model of ovalbumin (OVA)-induced asthma. CuONPs markedly increased airway hyper-responsiveness (AHR), inflammatory cell counts, proinflammatory cytokines and reactive oxygen species (ROS). CuONPs induced airway inflammation and mucus secretion with increases in phosphorylation of the MAPKs (Erk, JNK and p38). In the OVA-induced asthma model, CuONPs aggravated the increased AHR, inflammatory cell count, proinflammatory cytokines, ROS and immunoglobulin E induced by OVA exposure. In addition, CuONPs markedly increased inflammatory cell infiltration into the lung and mucus secretions, and MAPK phosphorylation was elevated compared to OVA-induced asthmatic mice. Taken together, CuONPs exhibited toxicity on the respiratory system, which was associated with the MAPK phosphorylation. In addition, CuONPs exposure aggravated the development of asthma. We conclude that CuONPs exposure has a potential toxicity in humans with respiratory disease.</P>

Characteristics of Occupational Exposure to Benzene during Turnaround in the Petrochemical Industries

Chung, Eun-Kyo,Shin, Jung-Ah,Lee, Byung-Kyu,Kwon, Ji-Woon,Lee, Na-Roo,Chung, Kwang-Jae,Lee, Jong-Han,Lee, In-Seop,Kang, Seong-Kyu,Jang, Jae-Kil Occupational Safety and Health Research Institute 2010 Safety and health at work Vol.1 No.1

Objectives: The level of benzene exposure in the petrochemical industry during regular operation has been well established, but not in turnaround (TA), where high exposure may occur. In this study, the characteristics of occupational exposure to benzene during TA in the petrochemical companies were investigated in order to determine the best management strategies and improve the working environment. This was accomplished by evaluating the exposure level for the workers working in environments where benzene was being produced or used as an ingredient during the unit process. Methods: From 2003 to 2008, a total of 705 workers in three petrochemical companies in Korea were studied. Long- and short-term (< 1 hr) samples were taken during TAs. TA was classified into three stages: shut-down, maintenance and start-up. All works were classified into 12 occupation categories. Results: The long-term geometric mean (GM) benzene exposure level was 0.025 (5.82) ppm (0.005-42.120 ppm) and the short-term exposure concentration during TA was 0.020 (17.42) ppm (0.005-61.855 ppm). The proportions of TA samples exceeding the time-weighted average, occupational exposure level (TWA-OEL in Korea, 1 ppm) and the short-term exposure limit (STEL-OEL, 5 ppm) were 4.1% (20 samples of 488) and 6.0% (13 samples of 217), respectively. The results for the benzene exposure levels and the rates of exceeding the OEL were both statistically significant (p < 0.05). Among the 12 job categories of petrochemical workers, mechanical engineers, plumbers, welders, fieldman and scaffolding workers exhibited long-term samples that exceeded the OEL of benzene, and the rate of exceeding the OEL was statistically significant for the first two occupations (p < 0.05). Conclusion: These findings suggest that the periodic work environment must be assessed during non-routine works such as TA.

Type III Secretion System of <i>Pseudomonas aeruginosa</i> Affects Matrix Metalloproteinase 12 (MMP-12) and MMP-13 Expression via Nuclear Factor κB Signaling in Human Carcinoma Epithelial Cells and a Pneumonia Mouse Model

Park, Ji-Won,Kim, Yong-Jae,Shin, In-Sik,Kwon, Ok-Kyoung,Hong, Ju Mi,Shin, Na-Rae,Oh, Sei-Ryang,Ha, Un-Hwan,Kim, Jae-Hong,Ahn, Kyung-Seop Oxford University Press 2016 The Journal of infectious diseases Vol.214 No.6

<P>The type III secretion system (T3SS) in Pseudomonas aeruginosa has been linked to severe disease and poor clinical outcomes in animal and human studies. We aimed to investigate whether the ExoS and ExoT effector proteins of P. aeruginosa affect the expression of matrix metalloproteinase 12 (MMP-12) and MMP-13 via nuclear factor kappa B (NF-kappa B) signaling pathways. To understand the T3SS, we used Delta ExoS, Delta ExoT, and ExsA::Omega mutants, as well as P. aeruginosa strain K (PAK)-stimulated NCI-H292 cells. We investigated the effects of Delta ExoS, Delta ExoT, and ExsA::Omega on the development of pneumonia in mouse models. We examined the effects of Delta ExoS, Delta ExoT, and ExsA::Omega on MMP-12 and MMP-13 production in NCI-H292 cells. Delta ExoS and Delta ExoT markedly decreased the neutrophil count in bronchoalveolar lavage fluid, with a reduction in proinflammatory mediators, MMP-12, and MMP-13. Delta ExoS and Delta ExoT reduced NF-kappa B phosphorylation, together with MMP-12 and MMP-13 expression in PAK-infected mouse models and NCI-H292 cells. To conclude, P. aeruginosa infection induced the expression of MMPs, and P. aeruginosa T3SS appeared to be a key player in MMP-12 and MMP-13 expression, which is further controlled by NF-kappa B signaling. These findings might be useful in devising a novel therapeutic approach to chronic pulmonary infections that involves decreasing the ExoS and ExoT levels.</P>

Feather-trap for Genus Monopis (Lepidoptera; Tineidae) from Korea

Dong-june Lee,Sol-moon Na,Hye-in Lee,Yang-seop Bae 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

Two species of genus Monopis (M. pavlovskii, M. congestella) from detritus were found in raptorial bird cage. We used feather-traps that include detritus of raptorial bird, each two traps deployed at Mt.Gyeyang in Incheon and Mt.Cheonchuk in Uljin, respectively. Larvae of tineid feed on keratin sources in the detritus of the raptorial bird cage such as pellets, furs, and feathers. Biological information regarding M. pavlovskii and M. congestella and photographs of their immature stage are provided. It is likely that detritus of raptorial bird cage provide a suitable habitat for keratophagous tineids in nature because of a wide variety of keratin in the raptorial bird cage.

Unconstrained Object Segmentation Using GrabCut Based on Automatic Generation of Initial Boundary

Na, In-Seop,Oh, Kang-Han,Kim, Soo-Hyung The Korea Contents Association 2013 International Journal of Contents Vol.9 No.1

Foreground estimation in object segmentation has been an important issue for last few decades. In this paper we propose a GrabCut based automatic foreground estimation method using block clustering. GrabCut is one of popular algorithms for image segmentation in 2D image. However GrabCut is semi-automatic algorithm. So it requires the user input a rough boundary for foreground and background. Typically, the user draws a rectangle around the object of interest manually. The goal of proposed method is to generate an initial rectangle automatically. In order to create initial rectangle, we use Gabor filter and Saliency map and then we use 4 features (amount of area, variance, amount of class with boundary area, amount of class with saliency map) to categorize foreground and background. From the experimental results, our proposed algorithm can achieve satisfactory accuracy in object segmentation without any prior information by the user.