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Effects of Nitrogen Sources and C/N Ratios on the Lipid-Producing Potential of Chlorella sp. HQ
( Jingjing Zhan ),( Yu Hong ),( Hongying Hu ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.6
Microalgae are being researched for their potential as attractive biofuel feedstock, particularly for their lipid production. For maximizing biofuel production, it is necessary to explore the effects of environmental factors on algal lipid-producing potential. In this study, the effects of nitrogen (N) sources (NO2-N, NO3-N, urea-N, NH4-N, and N-deficiency) and carbon-tonitrogen ratios (C/N= 0, 1.0, 3.0, and 5.0) on algal lipid-producing potential of Chlorella sp. HQ were investigated. The results showed that for Chlorella growth and lipid accumulation potential, NO2-N was t he b est amongst t he n itrogen sources, a nd N O3-N and u rea-N also contributed to algal growth and lipid accumulation potential, but NH4-N and N-deficiency instead caused inhibitory effects. Moreover, the results indicated that algal lipid-producing potential was related to C/N ratios. With NO2-N treatment and carbon addition (C/N = 1.0, 3.0, and 5.0), total lipid yield was enhanced by 12.96-20.37%, but triacylglycerol (TAG) yields decreased by 25.52-94.31%. As for NO3-N treatment, carbon addition led to a 17.82-57.43%/ 25.86-82.67% reduction of total lipid/TAG yields. When NH4-N was used as the nitrogen source, total lipid/TAG yields were increased by 46.67-113.33%/28.99-74.76% with carbon addition. The total lipid/TAG yields of urea-N treatment varied with C/N ratios. Overall, the highest TAG yield (TAG yield: 38.75 ± 5.21 mg/l; TAG content: 44.16 ± 4.35%) was achieved under NO2-N treatment without carbon addition (C/N = 0), the condition that had merit for biofuel production.
Effects of Nitrogen Sources and C/N Ratios on the Lipid-Producing Potential of Chlorella sp. HQ
Zhan, Jingjing,Hong, Yu,Hu, Hongying The Korean Society for Microbiology and Biotechnol 2016 Journal of microbiology and biotechnology Vol.26 No.7
Microalgae are being researched for their potential as attractive biofuel feedstock, particularly for their lipid production. For maximizing biofuel production, it is necessary to explore the effects of environmental factors on algal lipid-producing potential. In this study, the effects of nitrogen (N) sources (NO<sub>2</sub>-N, NO<sub>3</sub>-N, urea-N, NH<sub>4</sub>-N, and N-deficiency) and carbon-to-nitrogen ratios (C/N= 0, 1.0, 3.0, and 5.0) on algal lipid-producing potential of Chlorella sp. HQ were investigated. The results showed that for Chlorella growth and lipid accumulation potential, NO<sub>2</sub>-N was the best amongst the nitrogen sources, and NO<sub>3</sub>-N and urea-N also contributed to algal growth and lipid accumulation potential, but NH<sub>4</sub>-N and N-deficiency instead caused inhibitory effects. Moreover, the results indicated that algal lipid-producing potential was related to C/N ratios. With NO<sub>2</sub>-N treatment and carbon addition (C/N = 1.0, 3.0, and 5.0), total lipid yield was enhanced by 12.96-20.37%, but triacylglycerol (TAG) yields decreased by 25.52-94.31%. As for NO<sub>3</sub>-N treatment, carbon addition led to a 17.82-57.43%/25.86-82.67% reduction of total lipid/TAG yields. When NH<sub>4</sub>-N was used as the nitrogen source, total lipid/TAG yields were increased by 46.67-113.33%/28.99-74.76% with carbon addition. The total lipid/TAG yields of urea-N treatment varied with C/N ratios. Overall, the highest TAG yield (TAG yield: 38.75 ± 5.21 mg/l; TAG content: 44.16 ± 4.35%) was achieved under NO<sub>2</sub>-N treatment without carbon addition (C/N = 0), the condition that had merit for biofuel production.
Removal of Uranium from Uranium Plant Wastewater Using Zero-Valent Iron in an Ultrasonic Field
Jing Li,Libo Zhang,Jinhui Peng,Jinming Hu,Lifeng Yang,Aiyuan Ma,Hongying Xia,Wenqian Guo,Xia Yu 한국원자력학회 2016 Nuclear Engineering and Technology Vol.48 No.3
Uranium removal from uranium plant wastewater using zero-valent iron in an ultrasonicfield was investigated. Batch experiments designed by the response surface methodology(RSM) were conducted to study the effects of pH, ultrasonic reaction time, and dosage ofzero-valent iron on uranium removal efficiency. From the experimental data obtained inthis work, it was found that the ultrasonic method employing zero-valent iron powdereffectively removes uranium from uranium plant wastewater with a uranium concentrationof 2,772.23 mg/L. The pH ranges widely from 3 to 7 in the ultrasonic field, and theprediction model obtained by the RSM has good agreement with the experimental results.