http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Yamaji, Minoru,Hakoda, Yuuma,Horimoto, Ami,Okamoto, Hideki Korean Society of Photoscience 2014 Rapid communication in photoscience Vol.3 No.4
Novel diphenylphenanthrenes (DPPs) were prepared by a photocyclization method, and the substituent effects of the phenyl groups on the photophysical properties of the phenanthrene chromophore were investigated based on measurements of fluorescence yields, lifetimes, and transient absorption. Fluorescence activities in DPPs are increased by introducing phenyl rings that can enhance the transition moment along the short axis of the phenanthrene skeleton. Intersystem crossing from the fluorescent states to the triplet manifolds is shown to be operative through the triplet-triplet absorption spectra obtained by laser photolysis techniques.
( Shun Tamaki ),( Mitsuhiko Yagi ),( Yuki Nishihata ),( Hideki Yamaji ),( Yasushi Shigeri ),( Tomohide Uno ),( Hiromasa Imaishi ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.3
The aromatic compound p-hydroxybenzoate (PHBA) is an important material with multiple applications, including as a building block of liquid crystal polymers in chemical industries. The cytochrome P450 (CYP) enzymes are beneficial monooxygenases for the synthesis of chemicals, and CYP53A15 from fungus Cochliobolus lunatus is capable of executing the hydroxylation from benzoate to PHBA. Here, we constructed a system for the bioconversion of benzoate to PHBA in Escherichia coli cells coexpressing CYP53A15 and human NADPH-P450 oxidoreductase (CPR) genes as a redox partner. For suitable coexpression of CYP53A15 and CPR, we originally constructed five plasmids in which we replaced the N-terminal transmembrane region of CYP53A15 with a portion of the N-terminus of various mammalian P450s. PHBA productivity was the greatest when CYP53A15 expression was induced at 20°C in 2×YT medium in host E. coli strain ΔgcvR transformed with an N-terminal transmembrane region of rabbit CYP2C3. By optimizing each reaction condition (reaction temperature, substrate concentration, reaction time, and E. coli cell concentration), we achieved 90% wholecell conversion of benzoate. Our data demonstrate that the described novel E. coli bioconversion system is a more efficient tool for PHBA production from benzoate than the previously described yeast system.
Kumada, Yoichi,Sakan, Yoshinobu,Kajihara, Hideyuki,Kihara, Mana,Kikuchi, Yasufumi,Yamaji, Hideki,Seong, Gi Hun,Katoh, Shigeo Elsevier 2009 Journal of bioscience and bioengineering Vol.107 No.1
<P><B>Abstract</B></P><P>Single-chain Fv antibody (scFv) having 2 types of polypeptide linkers with or without rare codons, namely scFv (G<SUB>4</SUB>S)<SUB>3</SUB><SUP>R</SUP> and scFv No.10 (with rare codons) and scFv (G<SUB>4</SUB>S)<SUB>3</SUB> and scFv No.10<SUP>NR</SUP> (without rare codon), were expressed under controllable conditions in batch and fed-batch fermentation, in order to compare volumetric productivity and specific productivity levels of scFvs as a soluble form. In batch fermentation, volumetric productivity levels of scFv (G<SUB>4</SUB>S)<SUB>3</SUB><SUP>R</SUP> and scFv No.10, namely scFvs having the rare codon linkers were 3–5 times higher than those of scFvs that had linkers without the rare codon. In fed-batch fermentation controlled by an exponential feeding system, the cell concentrations of the transformants increased with similar specific growth rates (0.1 h<SUP>−1</SUP>), while the specific productivity levels of scFvs with the rare codon linkers were 1.6 times higher than those of scFvs without the rare codon linkers. These results indicate that the presence of several rare codons in the gene of a polypeptide linker increases soluble amount of scFvs. This might be caused by a temporary decrease in translation speed at the position of the polypeptide linker allowing time for the folding of the V<SUB>H</SUB> domain and avoiding unfavorable interactions between amino acid residues at the unfolded V<SUB>H</SUB> and V<SUB>L</SUB> domains. Higher specific productivity levels of both scFv No. 10 and scFv No. 10<SUP>NR</SUP> than those of scFv (G<SUB>4</SUB>S)<SUB>3</SUB><SUP>R</SUP> and (G<SUB>4</SUB>S)<SUB>3</SUB> might be caused by difference in stability of the polypeptide linkers on the basis of amino acid sequences. Thus, the rare codon linkers tested in this study will be considerably useful for large-scale production of soluble and active scFvs in fed-batch or continuous fermentations, in which high cell activity can be maintained.</P>
Akiyama, Yoko,Li, Suqin,Akiyama, Koshiro,Mori, Tatsuya,Okada, Hidehiko,Hirota, Noriyuki,Yamaji, Tsuyoshi,Matsuura, Hideki,Namba, Seitoku,Sekine, Tomokazu,Mishima, Fumihito,Nishijima, Shigehiro The Korean Society of Superconductivity and Cryoge 2021 한국초전도저온공학회논문지 Vol.23 No.3
The reduction of carbon dioxide emissions becomes a global issue, the main source of carbon dioxide emissions in the Asian region is the energy conversion sector, especially coal-fired power plants. We are working to develop technologies that will at least limit the increase in carbon dioxide emissions from the thermal power plants as one way to reduce carbon dioxide emissions. Our research aims to reduce carbon dioxide emissions by removing iron oxide scale from the feedwater system of thermal power plants using a superconducting high-gradient magnetic separation (HGMS) system, thereby reducing the loss of power generation efficiency. In this paper, the background of thermal power plants in Asia is outlined, followed by a case study of the introduction of a chemical cleaning line at an actual thermal power plant in Japan, and the possibility of introducing it into the thermal power plants in China based on the results.
Yoko Akiyama,Suqin Li,Koshiro Akiyama,Tatsuya Mori,Hidehiko Okada,Noriyuki Hirota,Tsuyoshi Yamaji,Hideki Matsuura,Seitoku Namba,Tomokazu Sekine,Fumihito Mishima,Shigehiro Nishijima 한국초전도저온공학회 2021 초전도와 저온공학 Vol.23 No.3
The reduction of carbon dioxide emissions becomes a global issue, the main source of carbon dioxide emissions in the Asian region is the energy conversion sector, especially coal-fired power plants. We are working to develop technologies that will at least limit the increase in carbon dioxide emissions from the thermal power plants as one way to reduce carbon dioxide emissions. Our research aims to reduce carbon dioxide emissions by removing iron oxide scale from the feedwater system of thermal power plants using a superconducting high-gradient magnetic separation (HGMS) system, thereby reducing the loss of power generation efficiency. In this paper, the background of thermal power plants in Asia is outlined, followed by a case study of the introduction of a chemical cleaning line at an actual thermal power plant in Japan, and the possibility of introducing it into the thermal power plants in China based on the results.