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RISS 인기검색어
- 검색키워드
- 저자 : ( Fatih Akdogan ) (검색결과 2 건)
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Hussein Abdullah Ahmed Ahmed,Selma Onarıcı,Allah Bakhsh,Guray Akdogan,Omer Cem Karakoc,Sancar Fatih Ozcan,Gulsum Aydın,Muhammad Aasım,Levent Unlu,Cengiz Sancak,Samir Naimov,Sebahattin Ozcan 한국식물생명공학회 2017 Plant biotechnology reports Vol.11 No.5
The expression of insecticidal genes must be induced at appropriate time and in sufficient amount to confer protection against targeted pests. However, the increased scientific reports of resistance development in insect pest against insecticidal delta-endotoxins, produced by Bacillus thuringiensis, provide impetus for the development of alternative insect management strategies. The present study was conducted to investigate the importance of targeted expression of a hybrid insecticidal gene (SN19) in potatoes. For this purpose, two plant expression vectors were constructed by cloning hybrid SN19 gene (cry1Badomain I–III and cry1Ia-domain II) under the control of a wound-inducible promoter isolated from Asparagus officinalis (AoPR1) and CaMV 35S promoter, and were transferred to Agrobacterium tumefaciens strain EHA 105. Four potato genotypes (Marabel, Innovator, Tokat 10/1 and Tokat 6/24) were transformed with EHA 105 strain harboring pTF101.1 35S–SN19 and pTF101.1 AoPR1–SN19 constructs. Phosphinothricin (PPT) was used at concentration of 1 mg/l for selection of primary transformants. PCR results showed the presence of both introduced SN19 and bar genes in 43 plants out of total 154 putative transgenics. Expression of SN19 protein in primary transformants was confirmed by Western blot assays. The mechanical wounding of transgenic plants exhibited more accumulated levels of SN19 proteins during post wounding period. Leaf biotoxicity assays with Colorado potato beetle (Coleoptera) and tomato leafminer (Lepidoptera) exhibited 100% mortality of the pests in primary transformants. Based on our mortality results with both constructs, we concluded that the potato transgenic lines exhibited targeted expression of insecticidal gene under the control of AoPR1 promoter upon insect wounding with eliminated toxicity of Cry protein and hence can be further used effectively in potato breeding programme.
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( Gulcin Gungor Olcum ),( Guven Koc ),( Fidan Canan Celik Yagan ),( Ece Yigit Taskin ),( Senem Ertilav ),( Hanife Serife Aktas ),( Sati Sena Yildiz ),( Demet Ataman Tasan ),( Fatih Akdogan ),( Sema Ba 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: RA is a chronic systemic disease manifest with multiple joint infi ammation. Vasculitis, heart, lung disease, extra-articular symptoms can also be present. TNF-a,IL-6 and mediators such as cytokines, have been shown to have an important role in this infi ammatory process. Anti-TNF drugs considered to be effective in preventing; RA,disability and joint destruction. CRP is one of the best indicators of infi ammation though, ESR is an indirect indicator of infi ammation and ESR levels affected by age, sex, status and anemia. ESR and CRP levels in patients with RA, disease activity and has been shown to correlate with radiographic fi ndings In this study, we aim to to show the correlation between ESR, CRP levels with disease activity in patients receiving anti-TNF alpha therapy. Methods: In this Retrospective study between January 2006 to March 2010 patients diagnosed with RA were evaluated in two groups. In the study group patients receiving at least one year of TNF-alpha were included where as in the control group patients only receiving DMARD were include. Only female patients were involved in both groups. There were 46 women in the study and 47 women in the control group. Disease severity and DAS 28 score was used to determine the disease activity. For statistical analysis Number Cruncher Statistical System 2007 & PASS 2008 Statistical Software (Utah,USA) was used. Results: In patients receiving anti-TNF alpha therapy DAS 28 scores showed statisticallysignifi cant correlation with the ESR. A statistically signifi cance between DAS 28 and CRP were not found. In control group with only DMARD treatment, the DAS 28.
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