Daucus carota Pentane-Based Fractions Suppress Proliferation and Induce Apoptosis in Human Colon Adenocarcinoma HT-29 Cells by Inhibiting the MAPK and PI3K Pathways

Wassim N. Shebaby,K.B. Bodman-Smith,Anthony Mansour,Mohamad Mroueh,Robin I. Taleb,Mirvat El-Sibai,Costantine F. Daher 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.7

Daucus carota L. ssp. carota (Apiacea, wild carrot, Queen Anne’s lace) has been used in folk medicine throughout the world and recently was shown to possess anticancer and antioxidant activities. This study aims to determine the anticancer activity of the pentane fraction (F1) and the 1:1 pentane:diethyl ether fraction (F2) of the Daucus Carota oil extract (DCOE) against human colon adenocarcinoma cell lines (HT-29 and Caco-2). Treatment of cells with various concentrations of F1 or F2 fractions produced a dose-dependent inhibition of cell proliferation. Flow cytometric analysis indicated that both fractions induced sub-G1 phase accumulation and increased apoptotic cell death. Western blot revealed the activation of caspase-3, PARP cleavage, and a considerable increase in Bax and p53 levels, and a decrease in Bcl-2 level. Treatment of HT-29 cells with either fraction markedly decreased the levels of both phosphorylated Erk and Akt. Furthermore, the combined treatment of F1 or F2 with wortmannin showed no added inhibition of cell survival suggesting an effect of F1 or F2 through the phosphatidyl inositol 3-kinase (PI3K) pathway. This study proposes that DCOE fractions (F1 and F2) inhibit cell proliferation by inducing cell cycle arrest and apoptosis in HT-29 cells through the suppression of mitogen-activated protein kinase (MAPK)/Erk and PI3K/Akt pathways.

A novel barium oxide-based Iraqi sand glass to attenuate the low gamma-ray energies: Fabrication, mechanical, and radiation protection capacity evaluation

Al-Saeedi F.H.F.,Sayyed M.I.,Kapustin F.L.,Al-Ghamdi Hanan,Kolobkova E.V.,Tashlykov O.L.,Almuqrin Aljawhara H.,Mahmoud K.A. 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.8

In the present work, untreated Iraqi sand with grain sizes varied between 100 and 200 mm was used to produce a colored glass sample that has shielding features against the low gamma-ray energy. Therefore, a weight of 70e60 wt % sand was mixed with 9e14 wt% B2O3, 8e10 wt% Na2O, 4e6 wt% of CaO, 3e6 wt% Al2O3, in addition to 0.3% of Co2O3. After melting and annealing the glass sample, the X-ray diffraction spectrometry was applied to affirm the amorphous phase of the fabricated glass samples. Moreover, the X-ray dispersive energy spectrometry was used to measure the chemical composition, and the MH-300A densimeter was applied to measure the fabricated sample's density. The Makishima-Makinzie model was applied to predict the mechanical properties of the fabricated glass. Besides, the Monte Carlo simulation was used to estimate the fabricated glass sample's radiation shielding capacity in the low-energy region between 22.1 and 160.6 keV. Therefore, the simulated linear attenuation coefficient changed between 10.725 and 0.484 cm1 , raising the gamma-ray energy between 22.1 and 160.6 keV. Also, other shielding parameters such as a half-value layer, pure lead equivalent thickness, and buildup factors were calculated

Energy Requirements in Early Life Are Similar for Male and Female Goat Kids

Bompadre, T.F.V.,Neto, O. Boaventura,Mendonca, A.N.,Souza, S.F.,Oliveira, D.,Fernandes, M.H.M.R.,Harter, C.J.,Almeida, A.K.,Resende, K.T.,Teixeira, I.A.M.A. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.12

Little is known about the gender differences in energetic requirements of goats in early life. In this study, we determined the energy requirements for maintenance and gain in intact male, castrated male and female Saanen goat kids using the comparative slaughter technique and provide new data on their body composition and energy efficiency. To determine the energy requirements for maintenance, we studied 21 intact males, 15 castrated males and 18 females ($5.0{\pm}0.1kg$ initial body weight (BW) and $23{\pm}5d$ of age) using a split-plot design with the following main factors: three genders (intact males, castrated males, and females) and three dry matter intake levels (ad libitum, 75% and 50% of ad libitum intake). A slaughter group included three kids, one for each nutritional plane, of each gender, and all three animals within a group were slaughtered when the ad libitum kid reached 15 kg in BW. Net energy requirements for gain were obtained for 17 intact males, eight castrated males and 15 females ($5.1{\pm}0.4kg$ BW and $23{\pm}13d$ of age). Animals were fed ad libitum and slaughtered when they reached 5, 10, and 15 kg in BW. A digestion trial was performed with nine kids of each gender to determine digestible energy, metabolizable energy and energy metabolizability of the diet. Our results show no effect of gender on the energy requirements for maintenance and gain, and overall net energy for maintenance was $205.6kJ/kg^{0.75}$ empty body weight gain (EBW) ($170.3kJ/kg^{0.75}$ BW) from 5 to 15 kg BW. Metabolizable energy for maintenance was calculated by iteration, assuming heat production equal to metabolizable energy intake at maintenance, and the result was $294.34kJ/kg^{0.75}$ EBW and $k_m$ of 0.70. As BW increased from 5 to 15 kg for all genders, the net energy required for gain increased from 9.5 to 12.0 kJ/g EBW gain (EWG), and assuming $k_g=0.47$, metabolizable energy for gain ranged from 20.2 to 25.5 kJ/g EWG. Our results indicate that it is not necessary to formulate diets with different energetic content for intact male, castrated male and female Saanen goat kids weighing from 5 to 15 kg.

Directional Limits on Persistent Gravitational Waves from Advanced LIGO’s First Observing Run

Abbott, B. P.,Abbott, R.,Abbott, T. D.,Abernathy, M. R.,Acernese, F.,Ackley, K.,Adams, C.,Adams, T.,Addesso, P.,Adhikari, R. X.,Adya, V. B.,Affeldt, C.,Agathos, M.,Agatsuma, K.,Aggarwal, N.,Aguiar, O. American Physical Society 2017 Physical Review Letters Vol.118 No.12

<P>We employ gravitational-wave radiometry to map the stochastic gravitational wave background expected from a variety of contributing mechanisms and test the assumption of isotropy using data from the Advanced Laser Interferometer Gravitational Wave Observatory's (aLIGO) first observing run. We also search for persistent gravitational waves from point sources with only minimal assumptions over the 20-1726 Hz frequency band. Finding no evidence of gravitational waves from either point sources or a stochastic background, we set limits at 90% confidence. For broadband point sources, we report upper limits on the gravitational wave energy flux per unit frequency in the range F-a circle minus (integral) < (0.1-56) x 10(-8) erg cm(-2) s(-1) Hz(-1) (f/25 Hz(a-1) depending on the sky location Theta and the spectral power index a. For extended sources, we report upper limits on the fractional gravitational wave energy density required to close the Universe of Omega(f,Theta < (0.39-7.6) x 10(-8) sr(-1) (f/25 Hz)(a) depending on Theta and a. Directed searches for narrowband gravitational waves from astrophysically interesting objects (Scorpius X- 1, Supernova 1987 A, and the Galactic Center) yield median frequency- dependent limits on strain amplitude of h(0) < (6.7, 5.5, and 7.0) x 10(-25), respectively, at the most sensitive detector frequencies between 130- 175 Hz. This represents a mean improvement of a factor of 2 across the band compared to previous searches of this kind for these sky locations, considering the different quantities of strain constrained in each case.</P>

Final report for the APMP.T-K4: Comparison of realizations of aluminium freezing-point temperatures

Gam, K S,Joung, W,Yamazawa, K,Cheung, C P,Kho, H Y,Wang, L,Tsai, S F,Norranim, U,Hafidzah, O,Gupta, J K Springer-Verlag 2013 Metrologia Vol.50 No.-

<P>The comparison APMP.T-K4 is the regional extension of the CCT-K4: an intercomparison of the realizations of the freezing-points of Al (660.323 °C) and Ag (961.78 °C). The comparison was organized in two loops and four sub-loops with high temperature standard platinum resistance thermometers (HTSPRTs) as transfer thermometers in the freezing-point comparisons. The comparison involved eight APMP NMIs (KRISS, NMIJ, SCL, NMC, CMS, NIMT, SIRIM, NPL), and KRISS and NMIJ acted as linking laboratories to the CCT-K4.</P><P>The transfer HTSPRTs showed a strong drift during the transportation between the NMIs. In the case of the Ag freezing-point comparison, the comparison results were scattered much more than expected. In the APMP meeting held in 2009, the participants agreed that the Ag comparison results would be omitted in the report. It revealed that the measurement results at the Al freezing-point of participants were in agreement with the key comparison reference value of the CCT-K4 within 4 mK except for one laboratory. Details of the comparison results, the uncertainty evaluation and the drift of the HTSPRTs are described in this report.</P><P>Main text.To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.</P><P>The final report has been peer-reviewed and approved for publication by the CCT, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).</P>

DICKSON INVARIANTS HIT BY THE STEENROD SQUARE

Tan, K.F.,Xu, Kai Korean Mathematical Society 2000 대한수학회보 Vol.37 No.4

Let $D_3$ be the Dickson invariant algebra of $F_2[X_1,\; X_2,\; X_3] \; by \; GL(3,\; F_2)$. In this paper, we provide an elementary proof of Theorem 3.2 of [2]; each element in $D_3$ is hit by the Steenrod square in $F_2[X_1,\; X_2,\; X_3]$.

Report of the CCQM-K97: measurement of arsenobetaine standard solution and arsenobetaine content in fish tissue (tunafish)

Ma, L D,Wang, J,WEI, C,Kuroiwa, T,Narukawa, T,Ito, N,HIOKI, A,CHIBA, K,Yim, Y H,Lee, K S,Lim, Y R,Turk, G C,Davis, C W,Mester, Z,Yang, L,McCooeye, M,Maxwell, P,Cankur, O,Tokman, N,Coskun, F G BUREAU INTERNATIONAL DES POIDS ET MESURES 2017 METROLOGIA -BERLIN- Vol.54 No.-

<P></P> <P>The CCQM-K97 key comparison was organized by the inorganic analysis working group (IAWG) of CCQM as a follow-up to completed pilot study CCQM-P96 and P96.1 to test the abilities of the national metrology institutes to accurately quantitate the mass fraction of arsenobetaine (AsB) in standard solution and in fish tissue. A pilot study CCQM-P133 was parallelized with this key comparison. National Institute of Metrology (NIM), China and National Metrology Institute of Japan (NMIJ) acted as the coordinating laboratories.</P> <P>Six NMIs participated in CCQM-K97 and two institutes participated in CCQM-P133, and all of them submitted the results. Some NMIs submitted more than one results by different methods. The results were in excellent agreement with each other, and obviously better than those of previous P96 and P96.1. Therefore the calibrant which each NMI used was comparable. It shows that the capabilities of some of the participants have been improved after the previous pilot studies.</P> <H2>Main text</H2> <P> To reach the main text of this paper, click on <A HREF='http://www.bipm.org/utils/common/pdf/final_reports/QM/K97/CCQM-K97.pdf'>Final Report</A>. Note that this text is that which appears in Appendix B of the BIPM key comparison database <A HREF='http://kcdb.bipm.org/'>kcdb.bipm.org/</A>.</P> <P>The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).</P>

Ambient Vibration measurements and finite element modelling for the Hong Kong Ting Kau Bridge

Au, F.T.K.,Tham, L.G.,Lee, P.K.K.,Su, C.,Han, D.J.,Yan, Q.S.,Wong, K.Y. Techno-Press 2003 Structural Engineering and Mechanics, An Int'l Jou Vol.15 No.1

The Ting Kau Bridge in Hong Kong is a cable-stayed bridge comprising two main spans and two side spans. The bridge deck is supported by three towers, an end pier and an abutment. Each of the three towers consists of a single reinforced concrete mast which reduces its section in steps, and it is strengthened by transverse cables and struts in the transverse vertical plane. The bridge deck is supported by four inclined planes of cables emanating from anchorages at the tower tops. In view of the threat from typhoons, the dynamic behaviour of long-span cable-supported bridges in the region is always an important consideration in their design. This paper is devoted to the ambient vibration measurements of the bridge for evaluation of dynamic characteristics including the natural frequencies and mode shapes. It also describes the modelling of the bridge. A few finite element models are developed and calibrated to match with the field data and the results of subsequent structural health monitoring of the bridge.

Genetic polymorphism in pvmdr1 and pvcrt-o genes in relation to in vitro drug susceptibility of Plasmodium vivax isolates from malaria-endemic countries

Lu, F.,Lim, C.S.,Nam, D.H.,Kim, K.,Lin, K.,Kim, T.S.,Lee, H.W.,Chen, J.H.,Wang, Y.,Sattabongkot, J.,Han, E.T. Verlag für Recht und Gesellschaft ; Elsevier 2011 Acta Tropica Vol.117 No.2

Treatment failure of chloroquine for Plasmodium vivax infection has increased in endemic countries. However, the molecular mechanisms for resistance and in vitro susceptibility of P. vivax to chloroquine remain elusive. We investigated the prevalence of mutations in the pvmdr1 and pvcrt-o genes, and the copy number of the pvmdr1 gene in isolates from the Republic of Korea (ROK), Thailand, the Union of Myanmar (Myanmar), and Papua New Guinea (PNG). We also measured in vitro susceptibility of Korean isolates to antimalarial drugs. The pvmdr1 analysis showed that mutations at amino acid position Y976F of pvmdr1 were found in isolates from Thailand (17.9%), Myanmar (13.3%), and PNG (100%), but none from the ROK, and mutation at position F1076L was present in isolates from the ROK (100%), Thailand (60.7%), and Myanmar (46.7%). One copy of the pvmdr1 gene was observed in most isolates and double copy numbers of the gene were observed in two Thai isolates. In the exons of the pvcrt-o gene that were sequenced, a K10 insertion was present in isolates from Thailand (56.0%) and Myanmar (46.2%), and the wild type was found in all Korean isolates. The results suggest that gene polymorphisms and copy number variation was observed in isolates of P. vivax from Southeast Asian countries. In Korean isolates polymorphism as limited to the F1076L variant, and no isolates with high level of resistance were found by in vitro susceptibility determinations. Moreover, our results provide a baseline for future prospective drug studies in malaria-endemic areas.

Effects of Crystalline Lysine, Threonine and Tryptophan Supplementation of Diets Containing Reduced Protein Levels on Performance of Growing Pigs

Li, D.F.,Xiao, C.T.,Kim, J.H.,Cho, W.T.,Han, In K. Asian Australasian Association of Animal Productio 1998 Animal Bioscience Vol.11 No.1

Three hundred and fifty-two growing pigs were utilized in three growth trials to investigate the effects of crystalline amino acid supplementation on growing pigs' growth performance and feed consumption. In Exp. 1, diets were formulated on the basis of digestible amino acids. Utilization of rapeseed meal plus cottonseed meal (R + C) in diet supported similar average daily gain (ADG), feed/gain (F/G) and cost per gain (p > 0.05) to group of soybean meal (SBM). In Exp. 2, 14% CP diets containing equal amount of cottonseed meal and rapeseed meal were formulated with the addition of threonine and/or tryptophan. Supplementation with threonine improved ADG and F/G (p < 0.05). Supplementation with only tryptophan made no benefits for ADG of growing pigs. In Exp. 3, diets with different threonine level were formulated. Increasing dietary threonine to 0.54% improved (p < 0.05) ADG and F/G compared to 0.45% dietary threonine. Increase in dietary threonine did not improve ADG (p > 0.05) but decreased F/G (p < 0.05) because of a decrease in average daily feed intake. Pigs fed diet with dietary threonine/lysine ratio of 67.5% supported optimum growth performance.