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Lee, Eujin,Kim, Sun-Gun,Park, Na-Young,Park, Hyo-Hyun,Jeong, Kyu-Tae,Choi, Jongkeun,Lee, In-Hae,Lee, Hwadong,Lee, Eunkyung Medknow PublicationsMedia Pvt Ltd 2017 Pharmacognosy magazine Vol.13 No.50
<P><B>Background:</B></P><P>A Korean herbal medicine, KOTMIN13, composed of <I>Inula japonica</I> Thunberg, <I>Trichosanthes kirilowii Maximowicz</I> var. <I>japonica kitamura</I>, <I>Peucedanum praeruptorum</I> Dunn, and <I>Allium macrostemon</I> Bge, has been used for anti-allergic and anti-asthmatic treatment in oriental clinics, but its activity has not been investigated.</P><P><B>Materials and Methods:</B></P><P>To evaluate the anti-inflammatory activity of KOTMIN13 for <I>in vitro</I> study, LPS-stimulated RAW 264.7 cells were used to induce the production and expression of inflammatory mediators and its mechanisms. 12-<I>O</I>-Tetradecanoylphorobol-13 aceate (TPA)-induced ear edema and carrageenan-induced paw edema models were also used to evaluate the effect of KOTMIN13 on acute inflammation <I>in vivo</I>.</P><P><B>Results:</B></P><P>KOTMIN13 reduced the release of inflammatory mediators [nitric oxide, prostaglandin E2, interleukin (IL)-1β, and IL-6] and the protein expression of inducible nitric oxide synthase and cyclooxygenase-2 in LPS-stimulated RAW 264.7 cells. Mechanism studies showed the attenuation of LPS-induced NF-κB activation by KOTMIN13 via IκBα degradation abrogation and a subsequent decrease in nuclear p65 levels. Activation of mitogen-activated protein kinases (ERK, JNK, and p38) was also suppressed. Furthermore, KOTMIN13 ameliorated the development of TPA-induced ear edema and carrageenan-induced paw edema in acute inflammatory edema mouse models.</P><P><B>Conclusion:</B></P><P>Our study demonstrates that KOTMIN13 inhibits inflammatory mediators through the inhibitions of NF-κB and MAPK activities in LPS-induced RAW 264.7 cells, as well as acute inflammation in edema models, indicating that KOTMIN13 is an effective suppressor for anti-inflammatory activities.</P><P><B>SUMMARY</B></P><P><P>KOTMIN13 decrease the production of No, PGE<SUB>2</SUB>, and proinflammatory cytokine (TNF-∝, IL-1β,IL-6).</P><P>KOTMIN13 Suppressed the degradation of NF-kβ and IKβα and the phosorylation of MAP Kinases.</P><P>Topical application of KOTMIN13 reduced mouse ear edema.</P><P>Oral administration of KOTMIN13 decreased carrageenan-induced paw edema.</P></P> >[FIG OMISSION]</BR><P><B>Abbreviations used:</B> NO: nitric oxide; PGE2: prostaglandin E2; iNOS: inducible NO synthase; COX-2: cyclooxygenase-2; TNF-α: tumor necrosis factor-α; IL: interleukin; NF-κB: nuclear factor kappaB; MAPK: mitogen-activated protein kinases; ERK: extracellular signal regulated kinase; JNK: c-jun N terminal kinase; TPA: 12-O-tetradecanoylphorbol-13-acetate</P>
이은경 ( Eunkyung Lee ),서민지 ( Minji Seo ),이경상 ( Kyeong-sang Lee ),최성원 ( Sungwon Choi ),이다래 ( Darae Lee ),진동현 ( Donghyun Jin ),권채영 ( Chaeyoung Kwon ),김홍희 ( Honghee Kim ),허모랑 ( Morang Huh ),한경수 ( Kyung-soo 대한원격탐사학회 2017 大韓遠隔探査學會誌 Vol.33 No.3
본 연구에서는 산출 방법에 따른 해빙 자료 간의 차이를 조사하기 위하여 1980-2010년 북극 지역의 EUMETSAT OSI SAF, NASA Team(NT)의 해빙 농도와 해빙 면적의 차이를 비교하였다. 그 결과 두 자료의 해빙 농도와 해빙 면적이 계절별, 해역별로 다른 일관성을 보였다. 계절별로는 OSI SAF의 해빙 농도가 전체적으로 0.85 %, 봄 0.48 %, 여름 0.97 %, 가을 1.38 %, 겨울 0.66 % 높게 나타났다. 해역별로는 북극해에서 OSI SAF의 해빙 농도가 2.7 %, 해빙 면적이 19.8만 ㎢ 높았으나 링컨해 일부에서는 해빙 농도가 2.3 %, 해빙 면적이 2만 ㎢ 낮게 나타났다. We compared sea ice concentration(SIC) and sea ice extent(SIE) using EUMETSAT Ocean and Sea Ice Satellite Application Facilities(OSI SAF) and NASA Team(NT) sea ice algorithm in the Arctic during 1980-2010 to investigate the difference between sea ice data applied different algorithms. SIC and SIE of the two data showed different consistency by season and by sea area. Seasonally, SIC of OSI SAF was 0.85 % overall, 0.48 % in spring, 0.97 % in summer, 1.38 % in autumn and 0.66 % in winter higher than NT SIC. By sea area, OSI SAF SIC was 2.7 %, SIE was 198,000 ㎢ higher than NT in Arctic Ocean, but in Lincoln Sea, OSI SAF SIC was 2.3 %, SIE was 20,000 ㎢ lower than NT.
Identification of Inducible Genes during Mast Cell Differentiation
Lee Eunkyung,Kang Sang-gu,Chang Hyeun Wook The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.2
Mast cells play an important role in allergic inflammation by releasing their bioactive mediators. The function of mast cells is enhanced by stimulation because of the induction of specific genes and their products. While many inducible genes have been elucidated, we speculated that a significant number of genes remain to be identified. Thus, we applied differential display (dd) PCR to establish a profile of the induced genes in bone marrow-derived mast cells (BMMCs) after they were co-cultured with 3T3 fibroblasts. To date, 150 cDNA fragments from the connective-type mast cells (CTMCs) were amplified. Among them, thirty cDNA fragments were reamplified for cloning and sequencing. The ddPCR strategy revealed that serine proteases were the most abundant genes among the sequenced clones induced during the maturation. Additionally, unknown genes from the co-culture of BMMCs with 3T3 fibroblasts were identified. We confirmed their induction in the CTMCs by Northern blot analysis and RT-PCR. Characterization of these induced genes during the maturation processes will provide insight into the functions of mast cells.
Lee, Hansol,Lee, Joonsung,Joe, Eunhae,Yang, Seungwook,Choi, Young-suk,Wang, Eunkyung,Song, Ho-Taek,Kim, Dong-Hyun Korean Society of Magnetic Resonance in Medicine 2015 Investigative Magnetic Resonance Imaging Vol.19 No.4
Purpose: For a single time-point hyperpolarized $^{13}C$ magnetic resonance spectroscopy imaging (MRSI) of animal models, scan-time window after injecting substrates is critical in terms of signal-to-noise ratio (SNR) of downstream metabolites. Pre-scans of time-resolved magnetic resonance spectroscopy (MRS) can be performed to determine the scan-time window. In this study, based on two-site exchange model, protocol-specific simulation approaches were developed for $^{13}C$ MRSI and the optimal scan-time window was determined to maximize the SNR of downstream metabolites. Materials and Methods: The arterial input function and conversion rate constant from injected substrates (pyruvate) to downstream metabolite (lactate) were precalibrated, based on pre-scans of time-resolved MRS. MRSI was simulated using two-site exchange model with considerations of scan parameters of MRSI. Optimal scan-time window for mapping lactate was chosen from simulated lactate intensity maps. The performance was validated by multiple in vivo experiments of BALB/C nude mice with MDA-MB-231 breast tumor cells. As a comparison, MRSI were performed with other scan-time windows simply chosen from the lactate signal intensities of pre-scan time-resolved MRS. Results: The optimal scan timing for our animal models was determined by simulation, and was found to be 15 s after injection of the pyruvate. Compared to the simple approach, we observed that the lactate peak signal to noise ratio (PSNR) was increased by 230%. Conclusion: Optimal scan timing to measure downstream metabolites using hyperpolarized $^{13}C$ MRSI can be determined by the proposed protocol-specific simulation approaches.
Effects of Cooling Rate on Precipitate Evolution and Residual Stresses in Al–Si–Mn–Mg Casting Alloy
Eunkyung Lee,Caitlin Walde,Brajendra Mishra 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.4
The residual stresses with Different heat treatment conditions have been measured and correlated with the microstructuralbehavior of AA365. 30 and 100 K/min cooling of AA365 inhibited the transformation of precipitates under 773 K, respectively. The alloy cooled at 30 and 100 K/min exhibited tensile residual stresses of 6.2 and 5.4 MPa, respectively, while thealloy cooled at 1 and 10 K/min showed compressive stresses of − 12.8 and − 10.3 MPa, respectively. The formation β′, β″,and other intermetallic compounds aff ected the compressive residual stresses, and that the fracture of the brittle intermetallicphases could reduce the extent of residual stresses in the lattice through plastic deformation.
Lee, Eunkyung,Choi, So-Young,Yang, Jae-Ho,Lee, Youn Ju The Korean Society of Pharmacology 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.4
Early life neuronal exposure to environmental toxicants has been suggested to be an important etiology of neurodegenerative disease development. Perfluorohexanesulfonate (PFHxS), one of the major perfluoroalkyl compounds, is widely distributed environmental contaminants. We have reported that PFHxS induces neuronal apoptosis via ERK-mediated pathway. Imperatorin is a furanocoumarin found in various edible plants and has a wide range of pharmacological effects including neuroprotection. In this study, the effects of imperatorin on PFHxS-induced neuronal apoptosis and the underlying mechanisms are examined using cerebellar granule cells (CGC). CGC were isolated from seven-day old rats and were grown in culture for seven days. Caspase-3 activity and TUNEL staining were used to determine neuronal apoptosis. PFHxS-induced apoptosis of CGC was significantly reduced by imperatorin and PD98059, an ERK pathway inhibitor. PFHxS induced a persistent increase in intracellular calcium, which was significantly blocked by imperatorin, NMDA receptor antagonist, MK801 and the L-type voltage-dependent calcium channel blockers, diltiazem and nifedipine. The activation of caspase-3 by PFHxS was also inhibited by MK801, diltiazem and nifedipine. PFHxS-increased ERK activation was inhibited by imperatorin, MK801, diltiazem and nifedipine. Taken together, imperatorin protects CGC against PFHxS-induced apoptosis via inhibition of NMDA receptor/intracellular calcium-mediated ERK pathway.
Lee, Min Kyung,Clay, Nicholas E.,Ko, Eunkyung,Smith, Cartney E.,Chen, Lin,Cho, Nicholas,Sung, Hak-Joon,DiPietro, Luisa,Lee, Jonghwi,Kong, Hyunjoon American Chemical Society 2018 Langmuir Vol.34 No.50
<P>Superparamagnetic iron oxide nanoparticles (SPIONs) are often encapsulated into drug-carrying nano/microsized particles for simultaneous magnetic resonance (MR) imaging and treatment of diseased tissues. Unfortunately, encapsulated SPIONs may have a limited ability to modulate the <I>T</I><SUB>2</SUB>-weighted relaxation of water protons, but this insight has not been examined systematically. This study demonstrates that SPIONs immobilized on 200 nm diameter poly(lactic-<I>co</I>-glycolic acid) (PLGA) nanoparticles using Pickering emulsification present 18-fold higher relaxivity than encapsulated SPIONs and 1.5-fold higher relaxivity than free SPIONs. In contrast, the SPIONs immobilized on 10 μm diameter PLGA particles exhibit a minor increase in MR relaxivity. This interesting finding will significantly impact current efforts to synthesize and assemble advanced MR contrast agents.</P> [FIG OMISSION]</BR>
Eunkyung Kang,Kyung Young Lee,Minwoo Lee,Sung-Byung Yang,Hwaran Lee Smart Tourism Research Center 2023 Journal of smart tourism Vol.3 No.2
Due to the phenomenon of aging, a new consumer segment known as the "new-silver generation" is emerging. Unlike the previous silver generation, this generation possesses significant economic power and consuming willingness, attracting attention from consumer goods companies. However, both the new-silver generation and the elderly face challenges in adopting contactless or self-service technologies such as self-order kiosks, resulting in negative reactions. Therefore, this study aims to investigate the attitude and response of the newsilver generation towards kiosks, as well as the factors influencing their resistance to such technology. By applying theoretical perspectives from the innovation resistance model, technostress theory, and the value-based model, this study identifies influencing factors for innovation resistance among the new-silver generation when using contactless technologies implemented in fast-food restaurants. The findings indicate that a lower awareness of new technologies and services corresponds to decreased adoption resistance, while a higher perceived value leads to more positive behaviors and attitudes among the new-silver generation utilizing kiosks at fast-food restaurants.