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( Nayoung Kim ),( Dong-hee Lee ),( Woo Seon Choi ),( Eunbi Yi ),( Hyojeong Kim ),( Jung Min Kim ),( Hyung-seung Jin ),( Hun Sik Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2021 BMB Reports Vol.54 No.1
Natural killer (NK) cells, key antitumor effectors of the innate immune system, are endowed with the unique ability to spontaneously eliminate cells undergoing a neoplastic transformation. Given their broad reactivity against diverse types of cancer and close association with cancer prognosis, NK cells have gained considerable attention as a promising therapeutic target for cancer immunotherapy. NK cell-based therapies have demonstrated favorable clinical efficacies in several hematological malignancies but limited success in solid tumors, thus highlighting the need to develop new therapeutic strategies to restore and optimize anti-tumor activity while preventing tumor immune escape. The current therapeutic modalities yielding encouraging results in clinical trials include the blockade of immune checkpoint receptors to overcome the immune-evasion mechanism used by tumors and the incorporation of tumor-directed chimeric antigen receptors to enhance NK cell anti-tumor specificity and activity. These observations, together with recent advances in the understanding of NK cell activation within the tumor microenvironment, will facilitate the optimal design of NK cell-based therapy against a broad range of cancers and, more desirably, refractory cancers. [BMB Reports 2021; 54(1): 44-58]
Expression of Ion Channels in Perivascular Stem Cells derived from Human Umbilical Cords
Kim, Eunbi,Park, Won Sun,Hong, Seok-Ho The Korean Society of Developmental Biology 2017 발생과 생식 Vol.21 No.1
Potassium channels, the largest group of pore proteins, selectively regulate the flow of potassium ($K^+$) ions across cell membranes. The activity and expression of $K^+$ channels are critical for the maintenance of normal functions in vessels and neurons, and for the regulation of cell differentiation and maturation. However, their role and expression in stem cells have been poorly understood. In this study, we isolated perivascular stem cells (PVCs) from human umbilical cords and investigated the expression patterns of big-conductance $Ca^{2+}$-activated $K^+$ ($BK_{Ca}$) and voltage-dependent $K^+$ ($K_v$) channels using the reverse transcription polymerase chain reaction. We also examined the effect of high glucose (HG, 25 mM) on expression levels of $BK_{Ca}$ and $K_v$ channels in PVCs. $K_{Ca}1.1$, $K_{Ca}{\beta}_3$, $K_v1.3$, $K_v3.2$, and $K_v6.1$ were detected in undifferentiated PVCs. In addition, HG treatment increased the amounts of $BK_{Ca}{\beta}_{3a}$, $BK_{Ca}{\beta}_4$, $K_v1.3$, $K_v1.6$, and $K_v6.1$ transcripts. These results suggested that ion channels may have important functions in the growth and differentiation of PVCs, which could be influenced by HG exposure.
( Eunbi Ko ),( Kyoungmin Cho ),( Ji Hye Kim ),( Hyun-jung Shin ),( Byung-fhy Suh ),( Hye One Kim ),( Taeyoung Park ) 한국피부장벽학회 2023 한국피부장벽학회지 Vol.25 No.2
Customers have used various skin care and functional cosmetics to prevent their skin aging as well as improve current condition of the skin. It is important to analyzing and understanding their skin to select optimized skin care solutions. The aim of this study is to quantitatively analyze the effect of environment, lifestyle, and innate genes on the current skin condition. For this purpose, degree of hydration, sebum, wrinkles, melanin, dullness and redness in a highly controlled condition were collected in conjunction with a questionnaire survey analyzing their lifestyles and genetic data from about 3000 women. We classified participants based on the types of skin, using 6 kinds of index representing skin properties, questionnaire survey on lifestyle and genetic data with Gaussian Mixture Model and Decision Tree for classification model. Through this study, it was possible to divide the skin of Korean women into 12 clusters according to wrinkles, melanin, redness, dullness and oil/moisture balance with Gaussian Mixture Model. Also, we were able to identify a pattern in which each factor was correlated. Next, we tried to discover factors for predicting skin condition changes through correlation analysis with lifestyle, climate/environment, and innate genes that affect the current skin condition. Ultimate purpose of this study is to predict future skin condition using big data and AI technology. This knowledge will enable us to provide more proactive and personalized solutions not only for cosmetics but also for life care such as lifestyle, eating habits, and environmental response.
Kim, Eunbi,Na, Sunghun,An, Borim,Yang, Se-Ran,Kim, Woo Jin,Ha, Kwon-Soo,Han, Eun-Taek,Park, Won Sun,Lee, Chang-Min,Lee, Ji Yoon,Lee, Seung-Joon,Hong, Seok-Ho The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.2
Understanding the crosstalk mechanisms between perivascular cells (PVCs) and cancer cells might be beneficial in preventing cancer development and metastasis. In this study, we investigated the paracrine influence of PVCs derived from human umbilical cords on the proliferation of lung adenocarcinoma epithelial cells (A549) and erythroleukemia cells (TF-$1{\alpha}$ and K562) in vitro using $Transwell^{(R)}$ co-culture systems. PVCs promoted the proliferation of A549 cells without inducing morphological changes, but had no effect on the proliferation of TF-$1{\alpha}$ and K562 cells. To identify the factors secreted from PVCs, conditioned media harvested from PVC cultures were analyzed by antibody arrays. We identified a set of cytokines, including persephin (PSPN), a neurotrophic factor, and a key regulator of oral squamous cell carcinoma progression. Supplementation with PSPN significantly increased the proliferation of A549 cells. These results suggested that PVCs produced a differential effect on the proliferation of cancer cells in a cell-type dependent manner. Further, secretome analyses of PVCs and the elucidation of the molecular mechanisms could facilitate the discovery of therapeutic target(s) for lung cancer.