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- 저자 : ( Daichi Nagaya ) (검색결과 1 건)
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Change of Phospholipid Species in Japanese Mustard Spinach Leaves during Senescence
( Daichi Nagaya ),( Daimon Syukri ),( Manasikan Thammawong ),( Kohei Nakano ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
In this study, a wide target analysis of phospholipid species in Japanese mustard spinach leaves stored for different durations was conducted by mass spectrometry, and phospholipid class distribution and fatty acid composition were compared for the purpose of identifying the freshness maker metabolites. Self-cultivated Japanese mustard spinach (Brassica rapa var. perviridis) leaves were harvested and stored for 4 and 7days at 20°C. Lipid in the leaves was extracted by a MTBE method and brought to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The multiple reaction monitoring (MRM) program for a total 141 phospholipids was prepared and used for a highly sensitive detection. Peak area of MRM chromatogram for each phospholipid molecule were calculated and compared using t-statistics. The highest number of phospholipids (43) were detected on day 7 whereas 41 was found on day 0. Almost all the phosphatidylcolines (PC) and phosphatidylinositols (PI) increased during senescence, but the other phospholipid classes were found unchanged. Especially, PI (14:1/16:1) and PC (32:1) increased significantly and were suggested to be a potential freshness marker metabolite. Further study is needed to confirm these findings through the quantitative determination.
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