망막전위도의 전력 산출

원철호,조진호,김상하,임정훈 경북대학교 의학연구소 1999 경북대학교병원의학연구소논문집 Vol.3 No.1

주파수영역에서 망막전위도의 총전력을 계산하고, 시간영역에서 망막전위도의 면적을계산할수 있는 프로그램을 개발함으로써 전기생리학적 진단의 효능을 향상시키는데 목적을 두었다. 총전력은 망막전위도 신호를 Fourier 변환을 이용하여 주파수 영역의 신호로 바꾼 다음 스펙트럼의 제곱을 적분함으로써 구하였다. 우세전력은 우세주파수의 전력으로 이부분의 전압을 제곱후 적분하여 얻은 값이다. 원하는 부분은 수동입력하여 구간주파수의 제곱적분을 할 수 있게 하였다. 이 소프트웨어는 시간 영역에서 진동양소파의 진폭의 합 및 면적도 구할 수 있는 기능을 가졌으며, 사용자에게 편리하도록 그래픽 환경 하에서 마우스와 팝업 메뉴에 의하여 각 기능들이 작동되며, UTAS-E2000 시스템과도 상호 호환성을 갖고 있다(한안지 38:817∼823, 1997). The Calculation of the Electroretinographic Power Chul Ho Won*, Jin Ho Cho, Ph.D.**, Sang Ha Kim, M. D., Jung Hoon Lim, M. D. To promote the electrophysiologic diagnostic efficiency, the program for calculation of the total power in the frequency domain and of the area in the time domain of the electroretinogram was designed. The total power is calculated by integration of the squared power spectrum obtained by Fourier transform (Parseval theorem) in the time domain. The dominant power is the power of the dominant frequency in power spectrum and is calculated by integration of the squared peak area spectrum. The sectional integration of the square power spectrum can be established by manual input. The implemented software also has the functions that calculate the summed amplitude and area of the oscillatory potentials in the time domain. And it performs each functions by selection of pop-up menu with mouse to use conveniently under graphic interface, compatible with UTAS-E2000 system (J Korean Ophthalmol Soc 38:817∼823, 1997).

金元 四大家에 關한 硏究

宋哲鎬,洪元植,金光湖 慶熙大學校韓醫科大學韓醫學硏究所 1986 慶熙韓醫大論文集 Vol.9 No.-

Study of decreased melanin production through p53 by heme oxygenase-1 inhibitor in normal human melanocytes

( Hee Sun Lim ),( Sun A Jin ),( Jee Bum Lee ),( Seong Jin Kim ),( Seung Chul Lee ),( Young Ho Won ),( Sook Jung Yoon ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Heme oxygenase (HO)-1 is induced by oxidative stress and plays important roles in anti-apoptosis and the rapid growth of several solid tumors. p53 has a central role in skin pigmentation and may impact onmelanoma at all stages. However, there has been little study of HO-1 in relation with p53 on melanin production Objectives: To know the effect of HO-1 on melanogenesis through p53 in normal human melanocytes Methods: Human melanocytes (hM) were primarily cultured from foreskin. After incubation, cells were rinsed twice with PBS then transfection with p53 siRNA Results: Melanin content was detected with ELISA and Western blot analysis and RT-PCR of tyrosinase, MITF were performed after ZnPP treatment and p53 transfection. After ZnPP treatment, melanin content was decreased, and tyrosinase and MITF protein levels were decreased. Tyrosinase and MITF mRNA levels were also decreased. However, melanin content and tyrosinase and MITF protein levels were increased after CoPP treatment. After p53 transfection, HO-1 expression was decreased when HO-1 stimulator was treated. In addition, melanin content was decreased, and tyrosinase expression was decreased in Western blot analysis Conclusion: These results suggest that melanogenesis is inhibited by ZnPP by decreased melanin production, tyrosinase and MITF protein and mRNA levels. Furthermore, those inhibitory effects of ZnPP may be dependent on p53 in normal human melanocytes. Therefore, HO-1 may play an important role in melanogensis

Upregulation of heme oxygenase-1 by ginsenoside Ro attenuates lipopolysaccharide-induced inflammation in macrophage cells

Sokho Kim,Myung-Hoon Oh,Bum-Seok Kim,Won-Il Kim,Ho-Seong Cho,Byoung-Yong Park,Chul Park,Gee-Wook Shin,Jungkee Kwon 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.4

Background: The beneficial effects of ginsenoside species have been well demonstrated in a number of studies. However, the function of ginsenoside Ro (GRo), an oleanane-type saponin, has not been suffi- ciently investigated. Thus, the aim of the present study was to investigate the anti-inflammatory effects of GRo in vitro using the Raw 264.7 mouse macrophage cell line treated with lipopolysaccharide (LPS), and to clarify the possible mechanism of GRo involving heme oxygenase-1 (HO-1), which itself plays a critical role in self-defense in the presence of inflammatory stress. Methods: Raw 264.7 cells were pretreated with GRo (up to 200mM) for 1 h before treatment with 1 mg/ mL LPS, and both cell viability and inflammatory markers involving HO-1 were evaluated. Results: GRo significantly increased cell viability in a dose dependent manner following treatment with LPS, and decreased levels of reactive oxygen species and nitric oxide. GRo decreased inflammatory cytokines such as nitric oxide synthase and cyclooxygenase-2 induced by LPS. Moreover, GRo increased the expression of HO-1 in a dose dependent manner. Cotreatment of GRo with tin protoporphyrin IX, a selective inhibitor of HO-1, not only inhibited upregulation of HO-1 induced by GRo, but also reversed the anti-inflammatory effect of GRo in LPS treated Raw 264.7 cells. Conclusion: GRo induces anti-inflammatory effects following treatment with LPS via upregulation of HO-1.

Upregulation of heme oxygenase-1 by ginsenoside Ro attenuates lipopolysaccharide-induced inflammation in macrophage cells

Kim, Sokho,Oh, Myung-Hoon,Kim, Bum-Seok,Kim, Won-Il,Cho, Ho-Seong,Park, Byoung-Yong,Park, Chul,Shin, Gee-Wook,Kwon, Jungkee The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.4

Background: The beneficial effects of ginsenoside species have been well demonstrated in a number of studies. However, the function of ginsenoside Ro (GRo), an oleanane-type saponin, has not been sufficiently investigated. Thus, the aim of the present study was to investigate the anti-inflammatory effects of GRo in vitro using the Raw 264.7 mouse macrophage cell line treated with lipopolysaccharide (LPS), and to clarify the possible mechanism of GRo involving heme oxygenase-1 (HO-1), which itself plays a critical role in self-defense in the presence of inflammatory stress. Methods: Raw 264.7 cells were pretreated with GRo (up to $200{\mu}M$) for 1 h before treatment with 1 mg/mL LPS, and both cell viability and inflammatory markers involving HO-1 were evaluated. Results: GRo significantly increased cell viability in a dose dependent manner following treatment with LPS, and decreased levels of reactive oxygen species and nitric oxide. GRo decreased inflammatory cytokines such as nitric oxide synthase and cyclooxygenase-2 induced by LPS. Moreover, GRo increased the expression of HO-1 in a dose dependent manner. Cotreatment of GRo with tin protoporphyrin IX, a selective inhibitor of HO-1, not only inhibited upregulation of HO-1 induced by GRo, but also reversed the anti-inflammatory effect of GRo in LPS treated Raw 264.7 cells. Conclusion: GRo induces anti-inflammatory effects following treatment with LPS via upregulation of HO-1.

Sulforaphane protects against acetaminophen-induced hepatotoxicity

Noh, Jung-Ran,Kim, Yong-Hoon,Hwang, Jung Hwan,Choi, Dong-Hee,Kim, Kyoung-Shim,Oh, Won-Keun,Lee, Chul-Ho Elsevier 2015 Food and chemical toxicology Vol.80 No.-

<P><B>Abstract</B></P> <P>Oxidative stress is closely associated with acetaminophen (APAP)-induced toxicity. Heme oxygenase-1 (HO-1), an antioxidant defense enzyme, has been shown to protect against oxidant-induced tissue injury. This study investigated whether sulforaphane (SFN), as a HO-1 inducer, plays a protective role against APAP hepatotoxicity <I>in vitro</I> and <I>in vivo</I>. Pretreatment of primary hepatocyte with SFN induced nuclear factor E2-factor related factor (Nrf2) target gene expression, especially HO-1 mRNA and protein expression, and suppressed APAP-induced glutathione (GSH) depletion and lipid peroxidation, which eventually leads to hepatocyte cell death. A comparable effect was observed in mice treated with APAP. Mice were treated with 300 mg/kg APAP 30 min after SFN (5 mg/kg) administration and were then sacrificed after 6 h. APAP alone caused severe liver injuries as characterized by increased plasma AST and ALT levels, GSH depletion, apoptosis, and 4-hydroxynonenal (4-HNE) formations. This APAP-induced liver damage was significantly attenuated by pretreatment with SFN. Furthermore, while hepatic reactive oxygen species (ROS) levels were increased by APAP exposure, pretreatment with SFN completely blocked ROS formation. These results suggest that SFN plays a protective role against APAP-mediated hepatotoxicity through antioxidant effects mediated by HO-1 induction. SFN has preventive action in oxidative stress-mediated liver injury.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SFN pretreatment increases the cell viability against APAP-induced toxicity. </LI> <LI> SFN pretreatment protects depletion of cellular GSH after APAP treatment. </LI> <LI> SFN pretreatment enhances Nrf2 target gene expression, especially HO-1 after APAP treatment. </LI> <LI> SFN has protective effect against APAP overdose-induced liver injury <I>in vivo</I> model as well. </LI> </UL> </P>

UASB 공정에 의한 고농도 축산폐수 처리시 유기물 제거와 메탄생성에 관한 연구

원철희,김승호,박은영,임재명 江原大學校 産業技術硏究所 2002 産業技術硏究 Vol.22 No.B

This research was performed to investigate the COD removal efficiency and methane production in slurry-typed swine wastes using UASB(upflow anaerobic sludge blanket)reactor. The USAB reactor was operated from 0.8 through 3.3days of HRT in a range of 3 to 15 kg TCOD/m³/day of volumetric organic loading rate. The removal rate of TCOD was increased with the increase of the HRT. The removal rate of TCOD at an HRT over 2days, became greater than 68% with the methane contents being from 70 to 80%. Methane production rates were increased from 0.27 to 0.36 m³CH₄/kg CODrem. as HRTs were increased from 0.8 to 3.3days.

X선 CT 영상으로부터 인체의 기관지 모델의 개발

원철호,노철균 한국센서학회 2002 센서학회지 Vol.11 No.5

본 논문에서는 X선 컴퓨터 단층 촬영으로부터 획득된 기관지의 3차원 영상으로부터 기관지의 구조 및 형태 정보를 추출할 수 있는 가능성을 조사하고, 분진 침착 모델의 수학적인 개발과 실험적인 검증에 사용될 수 있는 물리적인 공동 주형 모델을 개발한다. CT로부터 획득된 영상으로부터 수학적 모폴로지 기법에 의하여 폐의 기관지 영역을 검출한다. 검출된 폐의 기관지 영역의 체적 데이터를 3차원 공간상에서 나타낼 수 있는 표면 데이터를 STL(streolithography) 파일로 변환한 후에, 고속 프로토타입 기기에 입력하여 3차원 입체 형상을 제작한다. 이때 기존의 기관지 팬텀과 실제 정상인으로부터 획득된 CT 영상으로부터 각각의 공동 주형 모델을 제작하게 된다. CT 스캔하여 얻어진 원래의 영상과 제작된 공동 주형 모델을 CT 스캔하여 얻어진 영상에서의 기관지 영역의 단면 직경을 비교하여 생성된 폐 기관지 모델의 유용성을 검증하였다. In this paper, we investigate the potential for retrieval of morphometric data from three dimensional images of conducting bronchus obtained by X-ray Computerized Tomography (CT) and to explore the potential for the use of rapid prototype machine to produce physical hollow bronchus casts for mathematical modeling and experimental verification of particle deposition models. We segment the bronchus of lung by mathematical morphology method from obtained images by CT. The surface data representing volumetric bronchus data in three dimensions are converted to STL(streolithography) file and three dimensional solid model is created by using input STL file and rapid prototype machine. Two physical hollow cast models are created from the CT images of bronchial tree phantom and living human bronchus. We evaluate the usefulness of the rapid prototype model of bronchial tree by comparing diameters of the cross sectional area bronchus segments of the original CT images and the rapid prototyping-derived models imaged by X-ray CT.

치과기공사 국가시험 과목 타당성 연구

김웅철,이원철,손영석,오세윤,김부섭,유진호,김지환 한국보건의료인국가시험원 2005 보건의료교육평가 Vol.2 No.1

This study prepared a basic framework for the development and improvement of Korean Dental Technicians' Licensing Examination, based on actual test questions. A peer review was conducted to ensure relevance to current practices in dental technology. For the statistical analysis, 1000 dental laboratory technicians were selected; specialists in dental laboratory technology (laboratory owners, educators, etc.) were involved in creating valid and reliable questions. Results indicated that examination subjects should be divided into three categories: basic dental laboratory theory, dental laboratory specialties, and a practical examination. To ensure relevance to current practice, there should be less emphasis on basic dental laboratory theory, including health-related laws, and more emphasis on dental laboratory specialties. Introduction to dental anatomy should be separated from oral anatomy and tooth morphology; and fixed prosthodontics should be separated from crown and bridge technology and dental ceramics technology. Removable orthodontic appliance technology should be renamed 'orthodontic laboratory technology'. There should be less questions related to health related law, oral anatomy, dental hygiene, dental materials science and inlay, while the distribution ratio of questions related to tooth morphology should be maintained. There should be a decrease in the distribution ratio of questions related to crown and bridge technology, dental ceramics technology, complete dentures and removable partial dentures technology, and orthodontic laboratory technology. In the practical examination, the current multiple choice test should be replaced with tooth carving using wax or plaster. In dental laboratory specialties, subjects related to contemporary dental laboratory technology should be included in the test items.

MSR 공정에서 CaO를 이용한 CO_(2)의 탄산화·탈 탄산화 반응 특성연구

류완호,이원묵,이철우 한밭대학교 에너지청정기술연구소 2004 에너지청정기술논문집 Vol.4 No.1

MSR(Methane steam reforming)공정 중 발생되는 CO_(2)를 제거하기 위하여 CaCO_(3)에 무기바인더를 이용하여 구형의 CaO 반응물질을 제조하였다. 반응물질의 특성을 알아보기 위하여 반응온도, 농도, 접촉시간 등의 운전조건에 대한 변화와 CO_(2)와의 반응성에 대하여 연속 흐름식 반응장치를 이용하여 실험하였다. 반응물질의 소성온도는 850℃에서 반응성이 가장 좋았지만 탈 탄산화 공정을 고려하여 950℃로 결정하였다. GHSV를 3000hr^(-1)으로 고정시켜 놓고 실험한 결과, 반응기의 온도는 800℃, 유입되는 CO_(2)의 농도는 10%에서 반응성이 가장 좋았다. The adsorbent was prepared to separate CO_(2) gas generated from MSR process. Characteristies of carbonation and decarbonation of the adsorbent have been examine continuous reactor system in respect to temperature, concentrations at contact time. The decarbonation of CO_(2) onto adsorbents slowly occurred in this the temperature range from 850℃ to 950℃. However, the maximum carbonation ability was obtained at GHSV of 3000hr^(-1), temperature 800℃ and CO_(2) concentration of 10%(N_(2) balance).