중국 셰일가스 개발 문제점과 지원정책 분석

이채영(Chaeyoung Lee),윤준일(Junil Yoon),이홍(Hong Lee),이영수(Youngsoo Lee),신창훈(Changhoon Shin) 한국가스학회 2015 한국가스학회지 Vol.19 No.2

세계 최대 셰일가스 부존국가인 중국은 자국 국영기업을 중심으로 셰일가스 자원의 개발과 기술 확보에 많은 노력을 기울이고 있다. 중국의 셰일산업은 정부의 적극적인 지원과 기술적인 측면에서의 빠른 성장세를 감안할 때 향후 발전 가능성은 높은 것으로 보이나, 복잡한 지질구조와 상대적으로 심부에 위치한 셰일지층의 특성과 수자원, 인프라 부족 등과 같은 문제점으로 인해 단기적인 발전은 어렵다는 분석도 동시에 있다. 중국은 우리와의 지정학적으로 밀접한 관계는 물론 셰일가스 산업이 아직 초기단계에 있어, 최근 국내에서도 관련기업들을 중심으로 관심이 증가하고 있다. 이에 본 연구에서는, 중국 셰일자원 개발산업과 유관산업의 현황과 문제점 및 중국정부를 중심으로 한 해결 노력과 지원정책 등을 조사, 분석하여 우리기업들이 중국 셰일산업의 미래를 조망하는 데 도움을 주고자 하였다. 나아가 향후 중국 셰일산업의 발전에 따른 영향과 우리의 대처, 협력 및 참여 방안 수립에 참고가 되고자 하였다. 결과적으로, 본 연구에서는 중국의 셰일가스 개발을 위한 다양한 문제점을 검토하고, 중국정부의 정책적 지원, 국영기업의 독자적 기술 확보에 대한 노력과 해외기업과의 협력 추진 및 해외자산 인수 등 다양한 분야의 활동을 조사, 분석하여 제시하였다. China holding the world largest shale resources, has been trying to develop their domestic shale gas fields mainly with its NOCs. Chinese shale industry looks likely to have high potential to grow in the future, considering the eager support of Chinese government and the rapid development of relevant technologies by NOCs. However, there are opposite opinions as well that Chinese shale gas could not play a positive short-term results because of the complexity of structural geology, inadequacy of water resources and related infrastructure. Recently, Korean companies began to be interseted in Chinese shale gas industry, because of the special relationships with Korean industries in terms of geographic proximity and better opportunities due to the early phase of shale gas business in China. In this study, it was tried to help those companies looking out of future Chinese shale gas industry that surveying current status and problems of Chinese shale gas industry and relevant industries and investigating some trials and policies driven by China government. As a result, the various and long-term problems in Chinese shale development were reviewed and the active supports and polices of Chinese government, NOCs trials for establishments of their independent technologies and the cooperation with foreign companies or M&As were also investigated.

Composite Interval Mapping of Quantitative Trait Loci with Granddaughter Design

Lee, Chaeyoung,Lee, Kwang Jeon 한국유전학회 2003 Genes & Genomics Vol.25 No.1

For outcrossing species with limited female fertility, granddaughter design has been effective to map quantitative trait loci (QTLs). In the design, the sons of informative grandfathers who are heterozygous for the genetic markers are genotyped, and phenotypes of quantitative traits are recorded on the daughters of these sons. The composite interval mapping (CIM) with the granddaughter design to identify QTLs was empirically evaluated through the simulation in this study. The estimates of likelifhood ratios, QTL effects, and linkage positions were better in the populations with a larger family size, but the differences were not large, which implied that the QTL detection did not largely depend on the family size. On the other hand, QTL mapping significantly depended on the number of families in the population. And, the balanced design performed better than the unbalanced. One of the two QTLs simulated in the current study was not detected in the unbalanced population with the heritability of 0.3. This recommended that, in order to detect QTLs, the design should not be used in the population when the heritability was 0.3 or lower. This study also showed that a higher level of heritability led to more significant likelihood ratios and more accurate estimates of QTL effects and positions, enhancing the ability to identify QTLs. Also, precision of the estimates was improved with a large heritability.

Gene expression profiling study of grapevine in response to the Pierce’s disease and drought stress

Chaeyoung Lee,Daejin Hyung,Jinhyun Kim,Yejin Jo,Jusuk Park,Dongwoon Yu,Hongkyu Choi 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Susceptible Vitis vinifera responds to Xylella infection with a massive redirection of gene transcription. This transcriptional response is characterized by increased transcripts for phenlypropanoid and flavonoid biosynthesis, ethylene production, adaptation to oxidative stress, and homologs of pathogenesis related (PR) proteins, and decreased transcripts for genes related to photosynthesis. In addition, the results suggest that susceptible genotypes respond to Xylella infection by induction of limited, but inadequate, defense response. We also compared the transcriptional and physiological response of plants treated by pathogen infection, low or moderate water deficit, or a combination of pathogen infection and water deficit. Although the transcriptional response of plants to Xylella infection was distinct from the response of healthy plants to moderate water stress, we observed synergy between water stress and disease, such that water stressed plants exhibit a stronger transcriptional response to the pathogen. This interaction was mirrored at the physiological level for aspects of water relations and photosynthesis, and in terms of the severity of disease symptoms and pathogen colonization, providing a molecular correlation of the classical concept with the disease triangle.

Genetic map construction of model legume, Medicago truncatula, and analysis of genomic co-linearity with M. sativa

Chaeyoung Lee,Daejin Hyung,Jinhyun Kim,Dongwoon Yu,Jeonghoon Park,Meejin Kim,Hyun-A So,Eunsook Jeong,Howon Chung,Youngsoo Chung,Dohoon Kim,Jaihun Lee,Hongkyu Choi 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

A core genetic map of the legume Medicago truncatula has been established by analyzing the segregation of 288 sequence-characterized genetic markers in an F2 population composed of 93 individuals. These molecular markers correspond to 141 ESTs, 80 BAC end sequence-tags, and 67 resistance gene analogs, covering 513 cM. In the case of EST-based markers we used an intron-targeted marker strategy, with primers designed to anneal in conserved exon regions and amplify across intron regions. Polymorphisms were significantly more frequent in intron vs exon regions, thus providing an efficient mechanism to map transcribed genes. Genetic and cytogenetic analysis produced eight well-resolved linkage groups, which have been previously correlated with eight chromosomes by means of FISH with mapped BAC clones. We anticipated that mapping of conserved coding regions would have utility for comparative mapping among legumes; thus 60 of the EST-based primer pairs were designed to amplify orthologous sequences across a range of legume species. As an initial test of this strategy, we used primers designed against M. truncatula exon sequences to rapidly map genes in Medicago sativa. The resulting comparative map, which includes 68 bridging markers, indicates that the two Medicago genomes are highly similar, and establishes the basis for a “Medicago” composite map.

Cross-species translation of abiotic stress-responsive genes between Arabidopsis thaliana and Medicago truncatula.

Chaeyoung Lee,Daejin Hyung,Jinhyun Kim,Dongwoon Yu,Yejin Jo,Jusuk Park,Eunji Kwon,Hongkyu Choi 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07

Cross-species translation of genomic information may play a crucial role in applying biological knowledge gained from one species to other genomes. To screen and identify a broad range of abiotic stress-responsive genes, we employed a diverse array of resources, including Arabidopsis databases (http://www.arabidopsis.org), expression profiling data and previously reported literatures. As a result, a total of 1,377 genes were identified and classified into 18 different functional criteria based on biological processes of gene ontology. The gene set was translated into M. truncatula, which is a representative model system in the Fabaceae, by identifying orthologous genes between these two genomes with a combination of tBlastx and BlastP analyses. It is shown that approximately 82% of genes were estimated to be translated between the two genomes below the E-value of 10-30. These orthologous loci were used to construct comparative maps by developing a user-friendly analysis platform, resulting in a total of 52 synteny blocks. Furthermore, to discover central genes by which control responses to the abiotic stresses, a combination of AraNet (http://www.functionanet.org) and the Cytoscape program was used for the gene network analysis. The analysis resulted in the identification of 240 potential key genes. We anticipate that these genes may impact molecular breeding programs by discovering trait-associated SNPs followed by marker development.

What Holds the Future of Quantitative Genetics? - A Review

Lee, Chaeyoung Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.2

Genetic markers engendered by genome projects drew enormous interest in quantitative genetics, but knowledge on genetic architecture of complex traits is limited. Complexities in genetics will not allow us to easily clarify relationship between genotypes and phenotypes for quantitative traits. Quantitative genetics guides an important way in facing such challenges. It is our exciting task to find genes that affect complex traits. In this paper, landmark research and future prospects are discussed on genetic parameter estimation and quantitative trait locus (QTL) mapping as major subjects of interest.

Integration of comparative genetic maps for ten legume species using gene-based cross-species markers

Chaeyoung Lee,Daejin Hyung,Jinhyun Kim,Yejin Jo,Jusuk Park,Dongwoon Yu,Hongkyu Choi 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

The legume family is the third largest group, including approximately 650 genera and 18,000 species, in the flowering plants and the second important crops to the Poaceae in the agricultural economy. Comparative analysis is a useful tool to understand cross-species genomic structure and alterations during organism’s evolutionary history. In this study, we constructed a composite comparative map of ten legume species, including Medicago truncatula, Medicago sativa, Lens culinaris, Pisum sativum, Lotus japonicus, Cicer arietinum, Vicia faba L, Vigna radiata, Phaseolus vulgaris and Glycine max. Of these species, M. truncatula, which is a representative model system, played a central role to develop the cross-genome amplifiable PCR gene markers for the purpose of transferring them to other related legume species. A total of 140 cross-species core markers were employed to analyze genomic colinearity across this broad array of legume species. The comparative map demonstrates a diverse array of evolutionary events, such as duplications, inversions and reciprocal translocations. It is anticipated that resulting maps would provide a broader insights into the lineage-specific genomic organization of these glalegoid/phaseoloid legumes, which are two clades containing almost all crop legumes of economic importance, and can further used for the molecular breeding through translating genomic information into other orphan legumes.

Predicting consensus sequence of pre-mRNA splicing signals in legume family

Chaeyoung Lee,Jin-Hyun Kim,Joo-Seok Park,Hong-kyu Choi 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

For purposes of studying intron structures and predicting consensus splice motifs, a total of 102 legume species were used to isolate introns across the family. Of 196 gene-targeted PCR primer pairs, we successfully amplified 118 intron-containing genes (60.2%) and obtained a total of 1,870 introns with an average size of 143 nucleotides. Species-based compilation of 5’- and 3’-splicing motifs showed lineage-specific conservation in each splicing motif. Compilation of the entire intron set permitted prediction of the consensus sequences of splicing signal motifs in legumes, AYGWGTABABGH and TVNC/TAGGHTV for the 5’- and 3’-splicing motifs, respectively. Interestingly, these consensus motifs are very similar to the corresponding splicing signals of two model systems, Arabidopsis and rice. This result is suggestive of conservation of pre-mRNA splicing mechanisms in higher plants. Multiple alignments of CALTL introns demonstrated that the region from the branch point to 3’ splice site was relatively more conserved than the region from5’ splice site to the branch point. Phylogenetic analysis demonstrated that each of three splicing motifs, 5’-splice sites, 3’-splice sits, and branch site, was relevant to evolutionary divergence of species and phylogenetically informative, suggesting that splice signal sequences would be useful as a potential tool for the molecular phylogenetic analysis.

Comprehensive studies of Grassmann manifold optimization and sequential candidate set algorithm in a principal fitted component model

Chaeyoung, Lee,Jae Keun, Yoo The Korean Statistical Society 2022 Communications for statistical applications and me Vol.29 No.6

In this paper we compare parameter estimation by Grassmann manifold optimization and sequential candidate set algorithm in a structured principal fitted component (PFC) model. The structured PFC model extends the form of the covariance matrix of a random error to relieve the limits that occur due to too simple form of the matrix. However, unlike other PFC models, structured PFC model does not have a closed form for parameter estimation in dimension reduction which signals the need of numerical computation. The numerical computation can be done through Grassmann manifold optimization and sequential candidate set algorithm. We conducted numerical studies to compare the two methods by computing the results of sequential dimension testing and trace correlation values where we can compare the performance in determining dimension and estimating the basis. We could conclude that Grassmann manifold optimization outperforms sequential candidate set algorithm in dimension determination, while sequential candidate set algorithm is better in basis estimation when conducting dimension reduction. We also applied the methods in real data which derived the same result.

Estimation of Interaction Effects among Nucleotide Sequence Variants in Animal Genomes

Lee, Chaeyoung,Kim, Younyoung Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.1

Estimating genetic interaction effects in animal genomics would be one of the most challenging studies because the phenotypic variation for economically important traits might be largely explained by interaction effects among multiple nucleotide sequence variants under various environmental exposures. Genetic improvement of economic animals would be expected by understanding multi-locus genetic interaction effects associated with economic traits. Most analyses in animal breeding and genetics, however, have excluded the possibility of genetic interaction effects in their analytical models. This review discusses a historical estimation of the genetic interaction and difficulties in analyzing the interaction effects. Furthermore, two recently developed methods for assessing genetic interactions are introduced to animal genomics. One is the restricted partition method, as a nonparametric grouping-based approach, that iteratively utilizes grouping of genotypes with the smallest difference into a new group, and the other is the Bayesian method that draws inferences about the genetic interaction effects based on their marginal posterior distributions and attains the marginalization of the joint posterior distribution through Gibbs sampling as a Markov chain Monte Carlo. Further developing appropriate and efficient methods for assessing genetic interactions would be urgent to achieve accurate understanding of genetic architecture for complex traits of economic animals.